MX2009013325A - Transfeccion y transduccion de plantas con base en nano acarreadores. - Google Patents

Transfeccion y transduccion de plantas con base en nano acarreadores.

Info

Publication number
MX2009013325A
MX2009013325A MX2009013325A MX2009013325A MX2009013325A MX 2009013325 A MX2009013325 A MX 2009013325A MX 2009013325 A MX2009013325 A MX 2009013325A MX 2009013325 A MX2009013325 A MX 2009013325A MX 2009013325 A MX2009013325 A MX 2009013325A
Authority
MX
Mexico
Prior art keywords
transduction
based plant
transfection
zygotic embryos
plant transfection
Prior art date
Application number
MX2009013325A
Other languages
English (en)
Inventor
Francois Eudes
Archana Chugh
Original Assignee
Ca Minister Agriculture & Food
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Minister Agriculture & Food filed Critical Ca Minister Agriculture & Food
Publication of MX2009013325A publication Critical patent/MX2009013325A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22

Abstract

La presente invención proporciona un nuevo método para la transducción y/o transfección de las células vegetales. Los péptidos que penetran la célula (CPPs) han sido empleadas de forma exitosa como nanoportadores para la entrega de proteínas y oligonucleótidos para microsporas de célula vegetal individual así como también embriones cigóticos multi-celulares. La eficiencia de la internalización de CPP y además la entrega de una carga macromolecular que se compone de una proteína y/o un oligonucleótido se puede mejorar mediante la permeabilización de los embriones cigóticos.
MX2009013325A 2007-06-07 2008-06-09 Transfeccion y transduccion de plantas con base en nano acarreadores. MX2009013325A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92900607P 2007-06-07 2007-06-07
PCT/CA2008/001112 WO2008148223A1 (en) 2007-06-07 2008-06-09 Nanocarrier based plant transfection and transduction

Publications (1)

Publication Number Publication Date
MX2009013325A true MX2009013325A (es) 2010-05-20

Family

ID=40093127

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009013325A MX2009013325A (es) 2007-06-07 2008-06-09 Transfeccion y transduccion de plantas con base en nano acarreadores.

Country Status (11)

Country Link
US (2) US8680366B2 (es)
EP (1) EP2181193B1 (es)
JP (1) JP2010532159A (es)
CN (1) CN101889091B (es)
AU (1) AU2008258254B2 (es)
BR (1) BRPI0814244B1 (es)
CA (1) CA2693525C (es)
MX (1) MX2009013325A (es)
RU (1) RU2485180C2 (es)
WO (1) WO2008148223A1 (es)
ZA (1) ZA200908635B (es)

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EP2181193A4 (en) 2010-08-04
AU2008258254B2 (en) 2014-07-03
JP2010532159A (ja) 2010-10-07
ZA200908635B (en) 2010-08-25
BRPI0814244B1 (pt) 2018-10-09
AU2008258254A1 (en) 2008-12-11
US20140182012A1 (en) 2014-06-26
RU2485180C2 (ru) 2013-06-20
WO2008148223A1 (en) 2008-12-11
CN101889091A (zh) 2010-11-17
CA2693525C (en) 2020-09-29
EP2181193B1 (en) 2017-10-25
BRPI0814244A2 (pt) 2014-10-21
US20110035836A1 (en) 2011-02-10
EP2181193A1 (en) 2010-05-05
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