SG10201811729PA - Membrane-penetrating peptides to enhance transfection and compositions and methods for using same - Google Patents
Membrane-penetrating peptides to enhance transfection and compositions and methods for using sameInfo
- Publication number
- SG10201811729PA SG10201811729PA SG10201811729PA SG10201811729PA SG10201811729PA SG 10201811729P A SG10201811729P A SG 10201811729PA SG 10201811729P A SG10201811729P A SG 10201811729PA SG 10201811729P A SG10201811729P A SG 10201811729PA SG 10201811729P A SG10201811729P A SG 10201811729PA
- Authority
- SG
- Singapore
- Prior art keywords
- cargo
- membrane
- compositions
- methods
- peptides
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
- A61K47/544—Phospholipids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
- A61K47/6455—Polycationic oligopeptides, polypeptides or polyamino acids, e.g. for complexing nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Abstract
MEMBRANE-PENETRATING PEPTIDES TO ENHANCE TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suit - able cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells. [Fig. 9B]
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361915429P | 2013-12-12 | 2013-12-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201811729PA true SG10201811729PA (en) | 2019-02-27 |
Family
ID=52273587
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201811729PA SG10201811729PA (en) | 2013-12-12 | 2014-12-12 | Membrane-penetrating peptides to enhance transfection and compositions and methods for using same |
SG11201604697TA SG11201604697TA (en) | 2013-12-12 | 2014-12-12 | Membrane-penetrating peptides to enhance transfection and compositions and methods for using same |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201604697TA SG11201604697TA (en) | 2013-12-12 | 2014-12-12 | Membrane-penetrating peptides to enhance transfection and compositions and methods for using same |
Country Status (11)
Country | Link |
---|---|
US (3) | US9856496B2 (en) |
EP (2) | EP3079723B1 (en) |
JP (2) | JP6912887B2 (en) |
KR (1) | KR102532559B1 (en) |
CN (2) | CN106456793B (en) |
AU (2) | AU2014361806B2 (en) |
BR (1) | BR112016013516A8 (en) |
CA (1) | CA2933561A1 (en) |
ES (1) | ES2808866T3 (en) |
SG (2) | SG10201811729PA (en) |
WO (1) | WO2015089487A1 (en) |
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EP2862084A4 (en) | 2012-06-15 | 2016-11-30 | Soft Machines Inc | A method and system for implementing recovery from speculative forwarding miss-predictions/errors resulting from load store reordering and optimization |
WO2013188754A1 (en) | 2012-06-15 | 2013-12-19 | Soft Machines, Inc. | A disambiguation-free out of order load store queue |
KR101826399B1 (en) | 2012-06-15 | 2018-02-06 | 인텔 코포레이션 | An instruction definition to implement load store reordering and optimization |
KR101996351B1 (en) | 2012-06-15 | 2019-07-05 | 인텔 코포레이션 | A virtual load store queue having a dynamic dispatch window with a unified structure |
US9856496B2 (en) | 2013-12-12 | 2018-01-02 | Life Technologies Corporation | Membrane-penetrating peptides to enhance transfection and compositions and methods for using same |
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JP6865207B2 (en) | 2015-07-13 | 2021-04-28 | ライフ テクノロジーズ コーポレーション | Systems and methods for improved transient protein expression in CHO cells |
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-
2014
- 2014-12-12 US US14/569,583 patent/US9856496B2/en active Active
- 2014-12-12 KR KR1020167018604A patent/KR102532559B1/en active IP Right Grant
- 2014-12-12 JP JP2016538724A patent/JP6912887B2/en active Active
- 2014-12-12 EP EP14821440.6A patent/EP3079723B1/en active Active
- 2014-12-12 SG SG10201811729PA patent/SG10201811729PA/en unknown
- 2014-12-12 AU AU2014361806A patent/AU2014361806B2/en active Active
- 2014-12-12 CA CA2933561A patent/CA2933561A1/en active Pending
- 2014-12-12 CN CN201480074785.5A patent/CN106456793B/en active Active
- 2014-12-12 EP EP20176262.2A patent/EP3756690A3/en active Pending
- 2014-12-12 CN CN202110386654.2A patent/CN113105559A/en active Pending
- 2014-12-12 ES ES14821440T patent/ES2808866T3/en active Active
- 2014-12-12 SG SG11201604697TA patent/SG11201604697TA/en unknown
- 2014-12-12 WO PCT/US2014/070176 patent/WO2015089487A1/en active Application Filing
- 2014-12-12 BR BR112016013516A patent/BR112016013516A8/en not_active Application Discontinuation
-
2017
- 2017-11-13 US US15/811,423 patent/US10760098B2/en active Active
-
2019
- 2019-09-17 JP JP2019168228A patent/JP6985347B2/en active Active
-
2020
- 2020-03-19 AU AU2020202002A patent/AU2020202002B2/en active Active
- 2020-08-28 US US17/006,629 patent/US20200392537A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
BR112016013516A8 (en) | 2020-05-19 |
KR102532559B1 (en) | 2023-05-16 |
JP2020015747A (en) | 2020-01-30 |
AU2020202002A1 (en) | 2020-04-09 |
WO2015089487A1 (en) | 2015-06-18 |
CN106456793B (en) | 2021-04-30 |
JP2017500856A (en) | 2017-01-12 |
US20150211021A1 (en) | 2015-07-30 |
AU2014361806A1 (en) | 2016-07-28 |
CN106456793A (en) | 2017-02-22 |
BR112016013516A2 (en) | 2017-08-08 |
AU2014361806B2 (en) | 2019-12-19 |
JP6985347B2 (en) | 2021-12-22 |
US20180163231A1 (en) | 2018-06-14 |
KR20160103024A (en) | 2016-08-31 |
CN113105559A (en) | 2021-07-13 |
US10760098B2 (en) | 2020-09-01 |
EP3079723B1 (en) | 2020-05-27 |
EP3756690A2 (en) | 2020-12-30 |
ES2808866T3 (en) | 2021-03-02 |
EP3079723A1 (en) | 2016-10-19 |
EP3756690A3 (en) | 2021-03-17 |
US9856496B2 (en) | 2018-01-02 |
AU2020202002B2 (en) | 2021-08-05 |
US20200392537A1 (en) | 2020-12-17 |
CA2933561A1 (en) | 2015-06-18 |
SG11201604697TA (en) | 2016-07-28 |
JP6912887B2 (en) | 2021-08-04 |
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