WO2014006452A3 - Rapid alkalinization factor peptides for delivery of nucleic acid molecules into cells - Google Patents

Rapid alkalinization factor peptides for delivery of nucleic acid molecules into cells Download PDF

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Publication number
WO2014006452A3
WO2014006452A3 PCT/IB2012/053416 IB2012053416W WO2014006452A3 WO 2014006452 A3 WO2014006452 A3 WO 2014006452A3 IB 2012053416 W IB2012053416 W IB 2012053416W WO 2014006452 A3 WO2014006452 A3 WO 2014006452A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptides
cells
delivery
nucleic acid
acid molecules
Prior art date
Application number
PCT/IB2012/053416
Other languages
French (fr)
Other versions
WO2014006452A2 (en
Inventor
Daniel Scherer DE MOURA
Márcio de Castro SILVA-FILHO
Juliano Rodrigo GUERREIRO
Juan Carlos Guerrero ABAD
Original Assignee
Universidade De São Paulo - Usp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidade De São Paulo - Usp filed Critical Universidade De São Paulo - Usp
Priority to PCT/IB2012/053416 priority Critical patent/WO2014006452A2/en
Publication of WO2014006452A2 publication Critical patent/WO2014006452A2/en
Publication of WO2014006452A3 publication Critical patent/WO2014006452A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention concerns rapid alkalization factors peptides (RALF peptides) that can be used for delivering nucleic acids to both plant and animal cells, in vivo or in vitro. Also provided are methods for introducing a molecule of interest into a plant or animal cell using RALF peptides for genetically modifying them, and kit s comprising said RALF peptides.
PCT/IB2012/053416 2012-07-04 2012-07-04 Rapid alkalinization factor peptides for delivery of nucleic acid molecules into cells WO2014006452A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2012/053416 WO2014006452A2 (en) 2012-07-04 2012-07-04 Rapid alkalinization factor peptides for delivery of nucleic acid molecules into cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2012/053416 WO2014006452A2 (en) 2012-07-04 2012-07-04 Rapid alkalinization factor peptides for delivery of nucleic acid molecules into cells

Publications (2)

Publication Number Publication Date
WO2014006452A2 WO2014006452A2 (en) 2014-01-09
WO2014006452A3 true WO2014006452A3 (en) 2014-03-13

Family

ID=49882530

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2012/053416 WO2014006452A2 (en) 2012-07-04 2012-07-04 Rapid alkalinization factor peptides for delivery of nucleic acid molecules into cells

Country Status (1)

Country Link
WO (1) WO2014006452A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2015133652A1 (en) * 2014-03-06 2017-04-06 国立研究開発法人理化学研究所 Plant transformation methods
CN114395020B (en) * 2021-12-31 2023-08-08 华南农业大学 Application of GmRALF1 protein in promoting phosphorus element absorption of plants
CN116515856A (en) * 2023-04-26 2023-08-01 华南农业大学 Application of GmRALF2 protein and encoding gene thereof in relieving toxicity of heavy metal cadmium to plants

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0704221A2 (en) * 1994-09-29 1996-04-03 Ajinomoto Co., Inc. A conjugate useful in gene transfer and a method of producing the same
WO2001060972A2 (en) * 2000-02-15 2001-08-23 Washington State University Research Foundation Novel peptides and methods of use
US20030217389A1 (en) * 2001-02-15 2003-11-20 Ryan Clarence A Novel peptides and methods of use
WO2006004955A2 (en) * 2004-06-30 2006-01-12 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics and phenotypes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0704221A2 (en) * 1994-09-29 1996-04-03 Ajinomoto Co., Inc. A conjugate useful in gene transfer and a method of producing the same
WO2001060972A2 (en) * 2000-02-15 2001-08-23 Washington State University Research Foundation Novel peptides and methods of use
US20030217389A1 (en) * 2001-02-15 2003-11-20 Ryan Clarence A Novel peptides and methods of use
WO2006004955A2 (en) * 2004-06-30 2006-01-12 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics and phenotypes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PEARCE G ET AL.: "Structure-activity studies of RALF, Rapid Alkalinization Factor, reveal an essential-YISY--motif.", PEPTIDES., vol. 31, no. 11, 2010, pages 1973 - 7., XP027408844 *
TABATA BERGONCI: "Anàlise funcional dos peptídeos RALF em Arabidopsis: avaliaçâo do efeito do hormônio brassinolide em plantas superexpressoras e silenciadas para os genes AtRALFl e AtRALF34. 2012.", DISSERTAÇÂO DE MESTRADO, 2012 *

Also Published As

Publication number Publication date
WO2014006452A2 (en) 2014-01-09

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