ZA200908635B - Nanocarrier based plant transfection and transduction - Google Patents

Nanocarrier based plant transfection and transduction

Info

Publication number
ZA200908635B
ZA200908635B ZA200908635A ZA200908635A ZA200908635B ZA 200908635 B ZA200908635 B ZA 200908635B ZA 200908635 A ZA200908635 A ZA 200908635A ZA 200908635 A ZA200908635 A ZA 200908635A ZA 200908635 B ZA200908635 B ZA 200908635B
Authority
ZA
South Africa
Prior art keywords
transduction
based plant
plant transfection
nanocarrier based
nanocarrier
Prior art date
Application number
ZA200908635A
Other languages
English (en)
Inventor
Francois Eudes
Archana Chugh
Original Assignee
Ca Minister Agriculture & Food
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Minister Agriculture & Food filed Critical Ca Minister Agriculture & Food
Publication of ZA200908635B publication Critical patent/ZA200908635B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ZA200908635A 2007-06-07 2009-12-07 Nanocarrier based plant transfection and transduction ZA200908635B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92900607P 2007-06-07 2007-06-07

Publications (1)

Publication Number Publication Date
ZA200908635B true ZA200908635B (en) 2010-08-25

Family

ID=40093127

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200908635A ZA200908635B (en) 2007-06-07 2009-12-07 Nanocarrier based plant transfection and transduction

Country Status (11)

Country Link
US (2) US8680366B2 (xx)
EP (1) EP2181193B1 (xx)
JP (1) JP2010532159A (xx)
CN (1) CN101889091B (xx)
AU (1) AU2008258254B2 (xx)
BR (1) BRPI0814244B1 (xx)
CA (1) CA2693525C (xx)
MX (1) MX2009013325A (xx)
RU (1) RU2485180C2 (xx)
WO (1) WO2008148223A1 (xx)
ZA (1) ZA200908635B (xx)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US8735650B2 (en) * 2007-11-13 2014-05-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of modulating production of phenylpropanoid compounds in plants
US8923125B2 (en) * 2008-10-24 2014-12-30 Qualcomm Incorporated Wireless network resource adaptation
JP2011067192A (ja) * 2009-08-25 2011-04-07 Sumitomo Chemical Co Ltd 植物の開花時期を制御する遺伝子、タンパク質およびその利用
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
IN2012DN02333A (xx) * 2009-10-16 2015-08-21 Dow Agrosciences Llc
PE20130649A1 (es) 2010-03-08 2013-07-13 Monsanto Technology Llc Moleculas polinucleotidicas para regulacion genetica en plantas
JP6022470B2 (ja) 2010-11-24 2016-11-09 タカラ バイオ ユーエスエー， インコーポレイテッド 分散型タンパク質形質導入ドメインを含む誘導性発現系転写モジュレータおよびその使用方法
WO2012075562A1 (en) * 2010-12-08 2012-06-14 National Research Council Of Canada Microspore transformation methodology by macroinjection
US20120190107A1 (en) * 2011-01-26 2012-07-26 Dwayne Bisgrove Enhanced protein transduction
CA2830531C (en) 2011-03-23 2019-10-15 Dow Agrosciences Llc Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants
CA2842722C (en) * 2011-08-04 2020-06-09 Francois Eudes Organelle targeting nanocarriers
EP2756084B1 (en) * 2011-09-13 2020-06-03 Monsanto Technology LLC Methods and compositions for weed control
BR112014005951A2 (pt) * 2011-09-13 2017-04-04 Monsanto Technology Llc métodos e composições para controle de erva daninha
CA2848695A1 (en) * 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and composition for weed control comprising inhibiting ppg oxidase
WO2013040057A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
BR112014005795A2 (pt) * 2011-09-13 2020-12-08 Monsanto Technology Llc métodos de controle de plantas, de redução da expressão de um gene de hppd de uma planta, de preparação de um nucleotídeo, e de identificação de polinucleotídeos úteis na modulação da expressão do gene de hppd no tratamento externo de uma planta, composições e cassete de expressão microbiana
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
CN107739737A (zh) * 2011-09-13 2018-02-27 孟山都技术公司 用于杂草控制的方法和组合物
MX348495B (es) * 2011-09-13 2017-06-14 Monsanto Technology Llc Metodos y composiciones para el control de malezas.
WO2013040033A1 (en) * 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
CN102441177A (zh) * 2011-12-05 2012-05-09 中国人民解放军军事医学科学院放射与辐射医学研究所 用于siRNA传递的纳米泡载体
RU2478711C1 (ru) * 2011-12-19 2013-04-10 Открытое акционерное общество "Институт стволовых клеток человека" Способ повышения эффективности вирусной трансдукции
JP6074771B2 (ja) * 2012-02-08 2017-02-08 国立大学法人 奈良先端科学技術大学院大学 フロリゲンの導入方法
US10723806B2 (en) 2012-02-27 2020-07-28 Riken Method of introducing nucleic acid into plant cells
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
CA2873828A1 (en) * 2012-05-24 2013-11-28 A.B. Seeds Ltd. Naked dsrna for silencing target molecules in plant seeds
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
IN2015DN03218A (xx) 2012-10-05 2015-10-02 Dow Agrosciences Llc
CA2888264A1 (en) 2012-10-18 2014-04-24 Monsanto Technology Llc Methods and compositions for plant pest control
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
EA032406B1 (ru) * 2013-01-01 2019-05-31 Эй.Би. СИДЗ ЛТД. СПОСОБЫ ВВЕДЕНИЯ дсРНК В СЕМЕНА РАСТЕНИЙ ДЛЯ МОДУЛЯЦИИ ЭКСПРЕССИИ ГЕНОВ
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
AR095232A1 (es) 2013-03-13 2015-09-30 Monsanto Technology Llc Métodos y composiciones para el control de malezas
UY35385A (es) 2013-03-13 2014-09-30 Monsanto Technology Llc ?métodos y composiciones para el control de malezas?.
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
IL307456A (en) 2013-03-15 2023-12-01 Cibus Us Llc Methods and compositions for increasing the efficiency of targeted gene modification using oligonucleotide-mediated gene repair
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
CN104073519A (zh) * 2013-03-27 2014-10-01 北京市农林科学院 细胞穿透肽介导外源基因转化芸薹属植物小孢子的方法
UA121197C2 (uk) 2013-04-05 2020-04-27 Доу Агросайенсіс Ллс Нуклеаза "цинкові пальці" для модифікацїї гена ahas та спосіб її використання
EP3008186B1 (en) 2013-06-14 2018-11-28 Cellectis Methods for non-transgenic genome editing in plants
BR112016000555B1 (pt) 2013-07-19 2022-12-27 Monsanto Technology Llc Método para controlar uma infestação da espécie de leptinotarsa em uma planta, composição inseticida e construção de dna recombinante
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US11773400B2 (en) 2013-08-22 2023-10-03 E.I. Du Pont De Nemours And Company Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof
GB201315321D0 (en) 2013-08-28 2013-10-09 Koninklijke Nederlandse Akademie Van Wetenschappen Transduction Buffer
AR098295A1 (es) 2013-11-04 2016-05-26 Monsanto Technology Llc Composiciones y métodos para controlar infestaciones de plagas y parásitos de los artrópodos
EP3066202B1 (en) 2013-11-04 2021-03-03 Dow AgroSciences LLC Optimal soybean loci
RU2019128647A (ru) 2013-11-04 2019-11-05 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Оптимальные локусы кукурузы
UA120502C2 (uk) 2013-11-04 2019-12-26 Дау Агросайєнсиз Елелсі Спосіб отримання трансгенної рослини маїсу
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
MX368629B (es) 2014-01-15 2019-10-08 Monsanto Technology Llc Metodos y composiciones para el control de malezas utilizando polinucleotidos de 5-enolpiruvilshikimato-3-fosfato sintasa (epsps).
EP3115456A4 (en) * 2014-03-06 2017-10-25 Riken Plant transformation method
FI3116305T3 (fi) 2014-03-14 2024-02-08 Cibus Us Llc Menetelmät ja koostumukset kohdennetun geenimuunnoksen tehokkuuden lisäämiseksi käyttämällä oligonukleotidivälitteistä geeninkorjausta
BR112016022711A2 (pt) 2014-04-01 2017-10-31 Monsanto Technology Llc composições e métodos para controle de pragas de inseto
AU2015280252A1 (en) 2014-06-23 2017-01-12 Monsanto Technology Llc Compositions and methods for regulating gene expression via RNA interference
WO2015200539A1 (en) 2014-06-25 2015-12-30 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
US10378012B2 (en) 2014-07-29 2019-08-13 Monsanto Technology Llc Compositions and methods for controlling insect pests
WO2016040030A1 (en) 2014-09-12 2016-03-17 E. I. Du Pont De Nemours And Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
PL3256589T3 (pl) 2015-01-22 2022-02-21 Monsanto Technology Llc Kompozycje i sposoby kontrolowania leptinotarsa
WO2016160389A1 (en) 2015-03-27 2016-10-06 E I Du Pont De Nemours And Company Soybean u6 small nuclear rna gene promoters and their use in constitutive expression of small rna genes in plants
US10883103B2 (en) 2015-06-02 2021-01-05 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
CN108024517A (zh) 2015-06-03 2018-05-11 孟山都技术公司 用于将核酸引入到植物中的方法和组合物
AU2016338785B2 (en) 2015-10-12 2022-07-14 E. I. Du Pont De Nemours And Company Protected DNA templates for gene modification and increased homologous recombination in cells and methods of use
US20180282763A1 (en) 2015-10-20 2018-10-04 Pioneer Hi-Bred International, Inc. Restoring function to a non-functional gene product via guided cas systems and methods of use
WO2017100158A1 (en) 2015-12-11 2017-06-15 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
EP3390631B1 (en) 2015-12-18 2020-04-08 Danisco US Inc. Methods and compositions for t-rna based guide rna expression
EA201891629A1 (ru) 2016-02-09 2019-03-29 Сибас Юс Ллс Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
BR112018076027A2 (pt) 2016-06-14 2019-03-26 Pioneer Hi-Bred International, Inc. método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
CA3018430A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
EP3601610A1 (en) 2017-03-30 2020-02-05 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
WO2018197520A1 (en) 2017-04-24 2018-11-01 Dupont Nutrition Biosciences Aps Methods and compositions of anti-crispr proteins for use in plants
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
US20200109408A1 (en) 2017-05-31 2020-04-09 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
US11555199B2 (en) 2017-09-19 2023-01-17 Tropic Biosciences UK Limited Modifying the specificity of plant non-coding RNA molecules for silencing gene expression
US20210206814A1 (en) * 2017-10-12 2021-07-08 Mayo Foundation For Medical Education And Research Methods and materials for gene editing
WO2019118463A1 (en) 2017-12-15 2019-06-20 Danisco Us Inc Cas9 variants and methods of use
CN118834883A (zh) 2018-01-09 2024-10-25 希博斯美国有限公司 防碎基因和突变
BR112020014808A2 (pt) * 2018-01-22 2020-12-08 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Método para transformar uma mitocôndria de uma célula vegetal para expressar uma enzima nitrogenase, célula vegetal geneticamente modificada, planta geneticamente modificada e método para gerar uma planta geneticamente modificada
CN112020554B (zh) 2018-02-23 2024-10-22 先锋国际良种公司 新颖cas9直系同源物
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
US20210337753A1 (en) 2018-06-07 2021-11-04 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization Methods of regenerating and transforming cannabis
CN112424365A (zh) 2018-06-07 2021-02-26 以色列国家农业部、农村发展农业研究组织·沃尔卡尼中心 核酸构建体及其使用方法
EP3894550A4 (en) 2018-12-14 2023-01-04 Pioneer Hi-Bred International, Inc. NEW CRISPR-CAS SYSTEMS FOR GENOME EDITING
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
GB202109586D0 (en) 2021-07-02 2021-08-18 Tropic Biosciences Uk Ltd Method for editing banana genes
JP2024524924A (ja) 2021-07-02 2024-07-09 トロピック バイオサイエンシーズ ユーケー リミテッド バナナ果実における褐変の遅延又は防止
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
EP4441208A1 (en) 2021-11-30 2024-10-09 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
WO2024036190A2 (en) 2022-08-09 2024-02-15 Pioneer Hi-Bred International, Inc. Guide polynucleotide multiplexing
WO2024123786A1 (en) 2022-12-06 2024-06-13 Pioneer Hi-Bred International, Inc. Methods and compositions for co-delivery of t-dnas expressing multiple guide polynucleotides into plants
WO2024196921A1 (en) 2023-03-20 2024-09-26 Pioneer Hi-Bred International, Inc. Cas polypeptides with altered pam recognition
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0001609B1 (de) 1977-10-20 1982-08-04 Skf Kugellagerfabriken Gmbh Verfahren zur Herstellung eines Wälzlagers mit einem in einer Aussenhülse angeordnetem Käfig und Käfig zur Durchführung des Verfahrens
DE19618797C2 (de) 1996-05-10 2000-03-23 Bertling Wolf Vehikel zum Transport molekularer Substanz
AT409381B (de) 2000-03-03 2002-07-25 Gloessl Josef Dr Xylosyltransferase-gen
KR20040053645A (ko) 2002-12-17 2004-06-24 학교법인 성균관대학 양이온성 올리고펩타이드를 이용한 식물 형질전환법
US20040147027A1 (en) * 2003-01-28 2004-07-29 Troy Carol M. Complex for facilitating delivery of dsRNA into a cell and uses thereof
KR100568457B1 (ko) 2003-07-22 2006-04-07 학교법인 성균관대학 양이온성 올리고펩타이드를 이용한 식물체로의 ｒｎａ전달 기법
US8507277B2 (en) * 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US20060014712A1 (en) 2004-05-30 2006-01-19 Cemines, Inc. Controlled delivery of therapeutic compounds

Also Published As

Publication number Publication date
US9382548B2 (en) 2016-07-05
CA2693525A1 (en) 2008-12-11
MX2009013325A (es) 2010-05-20
EP2181193A1 (en) 2010-05-05
CN101889091A (zh) 2010-11-17
AU2008258254B2 (en) 2014-07-03
RU2009148877A (ru) 2011-07-20
BRPI0814244B1 (pt) 2018-10-09
CA2693525C (en) 2020-09-29
WO2008148223A1 (en) 2008-12-11
US20110035836A1 (en) 2011-02-10
US20140182012A1 (en) 2014-06-26
AU2008258254A1 (en) 2008-12-11
JP2010532159A (ja) 2010-10-07
CN101889091B (zh) 2014-02-19
EP2181193A4 (en) 2010-08-04
EP2181193B1 (en) 2017-10-25
RU2485180C2 (ru) 2013-06-20
BRPI0814244A2 (pt) 2014-10-21
US8680366B2 (en) 2014-03-25

Similar Documents

Publication Publication Date Title
ZA200908635B (en) Nanocarrier based plant transfection and transduction
HK1214943A1 (zh) 人工淚液及治療用途
EP2069496A4 (en) SELEX AND PHOTOSELEX ENHANCED
IL205571A0 (en) 4-pyrazolyl-n-arylpyrimidin-2-amines and 4-pyrazolyl-n-heteroarylpyrimidin
EP2152891A4 (en) GENES AND THEIR USES FOR PLANT ENHANCEMENT
PL2150617T3 (pl) Wzmocniona ekspresja i regiony stabilności
EP2185692A4 (en) HCO32 AND HCO27 AND RELATED EXAMPLES
EP2515946A4 (en) NANOCONJUGATES AND COMPOSITIONS OF NANOCONJUGATES
HK1148915A1 (en) Hair-supplementing and styling hairpiece
EP2467162A4 (en) THERAPEUTIC PROCESSES AND RELATED COMPOSITIONS
ZA201007138B (en) Plant regulatory elements and uses thereof
EP2200638A4 (en) TRANSFECTION PROCEDURES AND COMPOSITIONS FOR THIS
EG25788A (en) Artificial teethridge and fang
HK1161720A1 (en) Dihydroetorphines and their preparation
EP2269612A4 (en) EYE DROP PREPARATION AND ITS USE
GB0724489D0 (en) Artificial muscles
IL202722A0 (en) 4-imidazolines and their use as antidepressants
HK1148937A1 (en) Improved liposomes and uses thereof
EP2118321A4 (en) GENCARTS OF HUMAN GENES ASSOCIATED WITH ENDOMETRIOSIS
GB2450540B (en) Spine twister
IL214850A0 (en) Low-maintenance lawn seed mixtures and use thereof
IL209220A0 (en) Plant extract and its therapeutic use
EP2461870A4 (en) THERAPEUTIC COMPOSITIONS AND METHOD
ZA201000357B (en) 2-Azetidinemethaneamines and 2-pyrrolidinemethaneamines as Taar-Ligands
EP2273266A4 (en) PARASPORIN-1 RECEPTOR AND USE THEREOF