MX168507B - Procedimiento para la produccion de proteinas - Google Patents

Procedimiento para la produccion de proteinas

Info

Publication number
MX168507B
MX168507B MX011116A MX1111688A MX168507B MX 168507 B MX168507 B MX 168507B MX 011116 A MX011116 A MX 011116A MX 1111688 A MX1111688 A MX 1111688A MX 168507 B MX168507 B MX 168507B
Authority
MX
Mexico
Prior art keywords
production
proteins
procedure
human plasminogen
novel
Prior art date
Application number
MX011116A
Other languages
English (en)
Inventor
Bernd Meyhack
Jutta Heim
Rolf Burgi
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878709081A external-priority patent/GB8709081D0/en
Priority claimed from IE329987A external-priority patent/IE81116B1/en
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of MX168507B publication Critical patent/MX168507B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere a un metodo para la producción de un activador del plasminógeno humano, de tipo uroquinasa, de una sola cadena, o uno de sus mutantes; caracterizado porque comprende cultivar, con condiciones apropiadas de nutrientes, una cepa de levadura, transformada con un vector híbrido que incluye una secuencia del control de expresión de la levadura, un segmento de ADN que consta de una primera secuencia de ADN que codifica un peptido de señal, corriente arriba de y en un marco de lectura con una segunda secuencia de ADN, que codifica para el activador madura de plasminógeno, de tipo uroquinasa, o uno de sus mutantes, dicho segmento de aDN está bajo el control transcripcional de la secuencia de control de expresión, y una secuencia de ADN que comprende señales de terminación de transcripción de un gene de levadura y aislar dicho activador de plasminógeno, de tipo uroquinasa o sus mutantes.
MX011116A 1987-04-15 1988-04-14 Procedimiento para la produccion de proteinas MX168507B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB878709081A GB8709081D0 (en) 1987-04-15 1987-04-15 Production of proteins
GB878714059A GB8714059D0 (en) 1987-04-15 1987-06-16 Production of proteins
IE329987A IE81116B1 (en) 1986-12-05 1987-12-04 Hybrid plasminogen activators

Publications (1)

Publication Number Publication Date
MX168507B true MX168507B (es) 1993-05-27

Family

ID=27263391

Family Applications (1)

Application Number Title Priority Date Filing Date
MX011116A MX168507B (es) 1987-04-15 1988-04-14 Procedimiento para la produccion de proteinas

Country Status (18)

Country Link
US (1) US5175105A (es)
EP (1) EP0288435B1 (es)
JP (1) JP2894696B2 (es)
AT (1) ATE102994T1 (es)
AU (1) AU604305B2 (es)
CA (1) CA1341306C (es)
DD (1) DD274053A5 (es)
DE (1) DE3888386T2 (es)
DK (1) DK203588A (es)
ES (1) ES2061719T3 (es)
FI (1) FI98219C (es)
HU (1) HU204557B (es)
IL (1) IL86058A (es)
MX (1) MX168507B (es)
MY (1) MY103358A (es)
NO (1) NO179046C (es)
NZ (1) NZ224243A (es)
PT (1) PT87230B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2634495B1 (fr) * 1988-07-01 1990-10-26 Chambon Pierre Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes
ES2185529T3 (es) * 1989-03-06 2003-05-01 Univ Texas T-pa resistente a la serpina; mutante; genes.
US5866413A (en) * 1989-03-06 1999-02-02 Board Of Regents Of The University Of Texas System Pai-1 mutants
EP0387380A1 (en) * 1989-03-17 1990-09-19 BEHRINGWERKE Aktiengesellschaft Mutants of urinary plasminogen activator, their production and use
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
SE9201544L (sv) * 1991-05-31 1992-12-01 Ciba Geigy Ag Saett att framstaella glykosyltransferaser
GB2256197B (en) * 1991-05-31 1995-11-22 Ciba Geigy Ag Yeast as host for expression of heterologous glycosyl transferase enzymes
JPH0823995A (ja) * 1994-07-14 1996-01-30 Green Cross Corp:The 宿主細胞由来のプロテアーゼで低分子化され得る蛋白質の製造方法
US20030113271A1 (en) * 1997-01-29 2003-06-19 University Technology Corporation Formulations for pulmonary delivery
US6207442B1 (en) * 1997-10-16 2001-03-27 Zymogenetics, Inc. Plasmid construction by homologous recombination

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4245051A (en) * 1978-03-30 1981-01-13 Rockefeller University Human serum plasminogen activator
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
NL8003402A (nl) * 1980-06-11 1982-01-04 Leuven Res & Dev Vzw Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking.
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US5010003A (en) * 1983-04-25 1991-04-23 Genentech, Inc. Use of yeast homologous signals to secrete heterologous proteins
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
DE3585097D1 (de) * 1984-02-27 1992-02-20 Green Cross Corp Herstellung menschlicher urokinase.
JPS60256385A (ja) * 1984-05-18 1985-12-18 イーライ・リリー・アンド・カンパニー 酵母においてヘテロローガスポリペプチドを効率よく発現するための方法、ベクターおよび形質転換体
JPS60248181A (ja) * 1984-05-23 1985-12-07 Shiseido Co Ltd 酵母発現ベクタ−
EP0174835A1 (en) * 1984-09-11 1986-03-19 The Upjohn Company Human tissue plasminogen activator and recombinant DNA compounds
DE3680838D1 (de) * 1985-01-25 1991-09-19 Sagami Chem Res Stabilisierte menschliche prourokinase.
IL78571A (en) * 1985-04-22 1991-06-30 Genentech Inc Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form
US5219569A (en) * 1985-04-22 1993-06-15 Genentech, Inc. Protease resistant urokinase
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
DE19736137C1 (de) * 1997-08-20 1998-10-01 Daimler Benz Ag Verfahren zum Starten eines Verbrennungsmotors

Also Published As

Publication number Publication date
EP0288435B1 (en) 1994-03-16
AU1462688A (en) 1988-10-20
US5175105A (en) 1992-12-29
EP0288435A1 (en) 1988-10-26
DK203588D0 (da) 1988-04-14
FI98219B (fi) 1997-01-31
HU204557B (en) 1992-01-28
JPS63296689A (ja) 1988-12-02
JP2894696B2 (ja) 1999-05-24
FI881713A0 (fi) 1988-04-13
PT87230B (pt) 1992-08-31
NO179046B (no) 1996-04-15
DK203588A (da) 1988-10-16
CA1341306C (en) 2001-10-23
AU604305B2 (en) 1990-12-13
NZ224243A (en) 1991-02-26
DD274053A5 (de) 1989-12-06
ES2061719T3 (es) 1994-12-16
DE3888386T2 (de) 1994-07-14
NO179046C (no) 1996-07-24
NO881615L (no) 1988-10-17
HUT46360A (en) 1988-10-28
MY103358A (en) 1993-06-30
FI881713A (fi) 1988-10-16
IL86058A0 (en) 1988-09-30
NO881615D0 (no) 1988-04-14
IL86058A (en) 1993-07-08
DE3888386D1 (de) 1994-04-21
FI98219C (fi) 1997-05-12
PT87230A (pt) 1988-05-01
ATE102994T1 (de) 1994-04-15

Similar Documents

Publication Publication Date Title
ATE232237T1 (de) Herstellung von funktionellen, menschlichen urokinaseproteinen
FI103987B1 (fi) Interleukiini-7
MX168507B (es) Procedimiento para la produccion de proteinas
AU3329989A (en) Viper venom polypeptides and genetic expression
NO853012L (no) Nye genetiske sekvenser, interfenonpeptider av type i uttrykt av disse og organismer som produserer dem.
NZ222793A (en) Hybrid t-pa, u-pa plasminogen activator with non natural glycosylation; vectors and transformed hosts
DK260188A (da) Enzymproduktion
ATE110112T1 (de) Verfahren und systeme zur herstellung von hiv- antigenen.