ATE102994T1 - Verfahren zur herstellung von proteinen. - Google Patents

Verfahren zur herstellung von proteinen.

Info

Publication number
ATE102994T1
ATE102994T1 AT88810234T AT88810234T ATE102994T1 AT E102994 T1 ATE102994 T1 AT E102994T1 AT 88810234 T AT88810234 T AT 88810234T AT 88810234 T AT88810234 T AT 88810234T AT E102994 T1 ATE102994 T1 AT E102994T1
Authority
AT
Austria
Prior art keywords
production
proteins
methods
human plasminogen
novel
Prior art date
Application number
AT88810234T
Other languages
English (en)
Inventor
Bernd Dr Meyhack
Jutta Dr Heim
Rolf Dr Buergi
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878709081A external-priority patent/GB8709081D0/en
Priority claimed from IE329987A external-priority patent/IE81116B1/en
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Application granted granted Critical
Publication of ATE102994T1 publication Critical patent/ATE102994T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT88810234T 1987-04-15 1988-04-11 Verfahren zur herstellung von proteinen. ATE102994T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB878709081A GB8709081D0 (en) 1987-04-15 1987-04-15 Production of proteins
GB878714059A GB8714059D0 (en) 1987-04-15 1987-06-16 Production of proteins
IE329987A IE81116B1 (en) 1986-12-05 1987-12-04 Hybrid plasminogen activators
EP88810234A EP0288435B1 (de) 1987-04-15 1988-04-11 Verfahren zur Herstellung von Proteinen

Publications (1)

Publication Number Publication Date
ATE102994T1 true ATE102994T1 (de) 1994-04-15

Family

ID=27263391

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88810234T ATE102994T1 (de) 1987-04-15 1988-04-11 Verfahren zur herstellung von proteinen.

Country Status (18)

Country Link
US (1) US5175105A (de)
EP (1) EP0288435B1 (de)
JP (1) JP2894696B2 (de)
AT (1) ATE102994T1 (de)
AU (1) AU604305B2 (de)
CA (1) CA1341306C (de)
DD (1) DD274053A5 (de)
DE (1) DE3888386T2 (de)
DK (1) DK203588A (de)
ES (1) ES2061719T3 (de)
FI (1) FI98219C (de)
HU (1) HU204557B (de)
IL (1) IL86058A (de)
MX (1) MX168507B (de)
MY (1) MY103358A (de)
NO (1) NO179046C (de)
NZ (1) NZ224243A (de)
PT (1) PT87230B (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2634495B1 (fr) * 1988-07-01 1990-10-26 Chambon Pierre Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes
ES2185529T3 (es) * 1989-03-06 2003-05-01 Univ Texas T-pa resistente a la serpina; mutante; genes.
US5866413A (en) * 1989-03-06 1999-02-02 Board Of Regents Of The University Of Texas System Pai-1 mutants
EP0387380A1 (de) * 1989-03-17 1990-09-19 BEHRINGWERKE Aktiengesellschaft Mutanten von Plasminogenaktivator aus Urin, ihre Herstellung und Verwendung
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
SE9201544L (sv) * 1991-05-31 1992-12-01 Ciba Geigy Ag Saett att framstaella glykosyltransferaser
GB2256197B (en) * 1991-05-31 1995-11-22 Ciba Geigy Ag Yeast as host for expression of heterologous glycosyl transferase enzymes
JPH0823995A (ja) * 1994-07-14 1996-01-30 Green Cross Corp:The 宿主細胞由来のプロテアーゼで低分子化され得る蛋白質の製造方法
US20030113271A1 (en) * 1997-01-29 2003-06-19 University Technology Corporation Formulations for pulmonary delivery
US6207442B1 (en) * 1997-10-16 2001-03-27 Zymogenetics, Inc. Plasmid construction by homologous recombination

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4245051A (en) * 1978-03-30 1981-01-13 Rockefeller University Human serum plasminogen activator
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
NL8003402A (nl) * 1980-06-11 1982-01-04 Leuven Res & Dev Vzw Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking.
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US5010003A (en) * 1983-04-25 1991-04-23 Genentech, Inc. Use of yeast homologous signals to secrete heterologous proteins
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
DE3585097D1 (de) * 1984-02-27 1992-02-20 Green Cross Corp Herstellung menschlicher urokinase.
JPS60256385A (ja) * 1984-05-18 1985-12-18 イーライ・リリー・アンド・カンパニー 酵母においてヘテロローガスポリペプチドを効率よく発現するための方法、ベクターおよび形質転換体
JPS60248181A (ja) * 1984-05-23 1985-12-07 Shiseido Co Ltd 酵母発現ベクタ−
EP0174835A1 (de) * 1984-09-11 1986-03-19 The Upjohn Company Menschlicher Gewebeplasminogenaktivator und rekombinante DNA-Zusammensetzungen
DE3680838D1 (de) * 1985-01-25 1991-09-19 Sagami Chem Res Stabilisierte menschliche prourokinase.
IL78571A (en) * 1985-04-22 1991-06-30 Genentech Inc Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form
US5219569A (en) * 1985-04-22 1993-06-15 Genentech, Inc. Protease resistant urokinase
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
DE19736137C1 (de) * 1997-08-20 1998-10-01 Daimler Benz Ag Verfahren zum Starten eines Verbrennungsmotors

Also Published As

Publication number Publication date
EP0288435B1 (de) 1994-03-16
AU1462688A (en) 1988-10-20
US5175105A (en) 1992-12-29
EP0288435A1 (de) 1988-10-26
DK203588D0 (da) 1988-04-14
FI98219B (fi) 1997-01-31
HU204557B (en) 1992-01-28
JPS63296689A (ja) 1988-12-02
JP2894696B2 (ja) 1999-05-24
FI881713A0 (fi) 1988-04-13
PT87230B (pt) 1992-08-31
NO179046B (no) 1996-04-15
DK203588A (da) 1988-10-16
MX168507B (es) 1993-05-27
CA1341306C (en) 2001-10-23
AU604305B2 (en) 1990-12-13
NZ224243A (en) 1991-02-26
DD274053A5 (de) 1989-12-06
ES2061719T3 (es) 1994-12-16
DE3888386T2 (de) 1994-07-14
NO179046C (no) 1996-07-24
NO881615L (no) 1988-10-17
HUT46360A (en) 1988-10-28
MY103358A (en) 1993-06-30
FI881713A (fi) 1988-10-16
IL86058A0 (en) 1988-09-30
NO881615D0 (no) 1988-04-14
IL86058A (en) 1993-07-08
DE3888386D1 (de) 1994-04-21
FI98219C (fi) 1997-05-12
PT87230A (pt) 1988-05-01

Similar Documents

Publication Publication Date Title
DE3382837D1 (de) Herstellung von funktionellen, menschlichen Urokinaseproteinen
ATE91714T1 (de) Transformation von trichoderma.
ATE199261T1 (de) Hybride immunglobuline
ES2142363T3 (es) Materiales y metodos para la hipersecrecion de aminoacidos.
DE3851035D1 (de) Interleukin-7.
AU3329989A (en) Viper venom polypeptides and genetic expression
FI90667C (fi) Menetelmä valmistaa uutta ihmisen tyypin I interferoniproteiinia
ATE102994T1 (de) Verfahren zur herstellung von proteinen.
DE3752298D1 (de) Gentechnische Herstellung von Faktor XIIIa
DE69430975D1 (de) Verfahren zur Herstellung von Proteinen
ATE196165T1 (de) Methoden zur herstellung von protein c
ATE110112T1 (de) Verfahren und systeme zur herstellung von hiv- antigenen.
EP0165759A3 (de) Säugetierpromotoren verwendbar in Hefeexpression