KR970021075A - 신규화합물과 그 용도 - Google Patents
신규화합물과 그 용도 Download PDFInfo
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- KR970021075A KR970021075A KR1019960043840A KR19960043840A KR970021075A KR 970021075 A KR970021075 A KR 970021075A KR 1019960043840 A KR1019960043840 A KR 1019960043840A KR 19960043840 A KR19960043840 A KR 19960043840A KR 970021075 A KR970021075 A KR 970021075A
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- 150000001875 compounds Chemical class 0.000 title claims abstract 9
- 210000001995 reticulocyte Anatomy 0.000 claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 5
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims abstract 27
- 125000000217 alkyl group Chemical group 0.000 claims abstract 24
- 229910052717 sulfur Inorganic materials 0.000 claims abstract 8
- 125000004434 sulfur atom Chemical group 0.000 claims abstract 8
- 125000002252 acyl group Chemical group 0.000 claims abstract 5
- 125000004432 carbon atom Chemical group C* 0.000 claims abstract 5
- 125000003545 alkoxy group Chemical group 0.000 claims abstract 4
- 150000001450 anions Chemical class 0.000 claims abstract 4
- 229910052799 carbon Chemical group 0.000 claims abstract 4
- 125000004430 oxygen atom Chemical group O* 0.000 claims abstract 4
- 230000002489 hematologic effect Effects 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 5
- 239000003093 cationic surfactant Substances 0.000 claims description 4
- 238000010186 staining Methods 0.000 claims 6
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 3
- 210000003743 erythrocyte Anatomy 0.000 claims 3
- 229920000447 polyanionic polymer Polymers 0.000 claims 3
- 210000001772 blood platelet Anatomy 0.000 claims 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- PLMFYJJFUUUCRZ-UHFFFAOYSA-M decyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)C PLMFYJJFUUUCRZ-UHFFFAOYSA-M 0.000 claims 1
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 claims 1
- 238000004043 dyeing Methods 0.000 claims 1
- 210000000267 erythroid cell Anatomy 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 229910052736 halogen Inorganic materials 0.000 claims 1
- 150000002367 halogens Chemical class 0.000 claims 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 230000001678 irradiating effect Effects 0.000 claims 1
- 210000000265 leukocyte Anatomy 0.000 claims 1
- 238000000691 measurement method Methods 0.000 claims 1
- XCOHAFVJQZPUKF-UHFFFAOYSA-M octyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCC[N+](C)(C)C XCOHAFVJQZPUKF-UHFFFAOYSA-M 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 239000004065 semiconductor Substances 0.000 claims 1
- 239000012128 staining reagent Substances 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 5
- 229940126062 Compound A Drugs 0.000 description 2
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
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Abstract
1. 청구범위에 기재된 발명이 속한 기술분야
본 발명은 신규화합물과 그 용도에 관한 것이다.
2. 발명이 해결하려고 하는 기술적과제
본 발명은 적색파장을 사용해서 망상적혈구를 정밀도가 양호하게 측정할 수 있는 신규의 화합물과 그 용도인 망상적혈구측정용의 시약 및 그 측정방법을 제공하는 것을 목적으로 한다.
3. 발명의 해결방법의 요지
식(Ⅰ)
(식중 R1은 수소원자, 또는 저급알킬기; R2및 R3은 수소원자 저급알킬기, 또는 저급알콕시기; R4는 수소원자, 아실기, 또는 저급알킬기; R5는 수소원자, 치환되어 있어도 되는 저급알킬기; Z는 유황원자, 산소원자, 또는 저급알킬기로 치환된 탄소원자; n은 1 또는 2; X-는 음이온이다)로 표시되는 화합물.
4. 발명의 중요한 용도
본 발명은 임상검사분야에 있어서의 망상적혈구 및 망상적혈구 성숙지수측정용을 위한 형광색소로서 사용가능한 신규화합물, 그 화합물을 포함하는 시약 및 망상적혈구의 측정방법에 관한 것이다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 색소화합물A를 포함하는 망상적혈구측정용시약을 사용해서 혈액학적시료의 전방산란광강도 및 형광강도를 측정한 경우의 산포도,
제2도는 본 발명의 색소화합물A를 포함하는 망상적혈구측정용시약을 다시또 양이온성계면활성제를 포함하는 시약을 사용해서 혈액학적시료의 전방산란광강도 및 형광강도를 측정한 경우의 산포도,
제3도는 본 발명의 색소를 포함하는 망상적혈구측정용시약을 사용해서 혈액학적시료의 전방산란광강도 및 형광강도를 측정한 경우의 산포도,
제4도는 본 발명의 색소를 포함하는 망상적혈구측정용시약을 사용해서 혈액학적시료의 전방산란광강도 및 형광강도를 측정한 경우의 산포도,
제5도는 본 발명의 색소를 포함하는 망상적혈구측정용시약을 사용해서 본 발명의 방법 및 종래의 R-2000을 사용한 방법에서 RET를 측정한 경우의 상관도.
Claims (14)
- 식(I)(식중 R1은 수소원자, 또는 저급알킬기; R2및 R3은 수소원자 저급알킬기, 또는 저급알콕시기; R4는 수소원자, 아실기, 또는 저급알킬기; R5는 수소원자, 치환되어 있어도 되는 저급알킬기; Z는 유황원자, 산소원자, 또는 저급알킬기로 치환된 탄소원자; n은 1 또는 2; X-는 음이온이다)로 표시되는 것을 특징으로 하는 화합물.
- 제1항에 있어서, 식(I)의 화합물에 있어서, R2및 R3는 수소원자; R4는 수소원자, 아실기, 또는 저급알킬기; R5는 수소원자, 치환되어 있어도 되는 저급알킬기인 것을 특징으로 하는 화합물.
- 제1항에 있어서, 식 (I)의 화합물에 있어서, R1은 메틸기; R2및 R3은 수소원자; R4및 R5는 수소원자; n=1; Z는 유황원자, R1은 메틸기; R2및 R3은 수소원자; R4는 수소원자; R5는 히드록시메틸기; n=1; Z는 유황원자, R1은 메틸기; R2및 R3은 수소원자; R4는 COCH3기; R5는 수소원자; n=1; Z는 유황원자, R1은 메틸기; R2및 R3은 수소원자; R4및 R5는 수소원자; n=2; Z는 유황원자인 것을 특징으로 하는 화합물.
- 식(I)(식중 R1은 수소원자 또는 저급알킬기; R2및 R3은 수소원자 저급알킬기, 또는 저급알콕시기; R4는 수소원자, 아실기 또는 저급알킬기; R5는 수소원자, 치환되어 있어도 되는 저급알킬기; Z는 유황원자, 산소원자, 또는 저급알킬기로 치환된 탄소원자; n은 1 또는 2; X-는 음이온이다)로 표시되는 화합물을 포함하는 것을 특징으로 하는 망상적혈구염색용시약.
- 제4항에 있어서, 적혈구의 비특이염색을 억제하기 위한 다가음이온과 완충제와를 포함하는 것을 특징으로 하는 망상적혈구염색용시약.
- 제5항에 있어서, 다가음이온이 황산이온, 인산이온, 다가카르복시산이온으로부터 선택된 적어도 1종인 것을 특징으로 하는 망상적헐구염색용시약.
- 제4항 내지 제6항중의 어느 한항에 있어서, pH가 6.0∼11.0인 것을 특징으로 하는 망상적혈구염색용시약.
- 제4항 내지 제7항중의 어느 한항에 있어서, 염색촉진제로서 양이온성계면활성제를 포함하는 것을 특징으로 하는 망상적혈구염색용시약.
- 양이온성계면활성제가(식중 R6은 탄소수 8∼12의 알킬기, R7, R8, 및 R9는 저급알킬기, X는 할로겐으로부터 선택된 적어도 하나이다)인 것을 특징으로 하는 망상적혈구염색용시약.
- 제8항에 있어서, 양이온성계면활성제가 라우릴트리메틸암모늄클로라이드, 데실트리메틸암모늄브로마이드, 옥틸트리메틸암모늄브로마이드의 적어도 1종인 것을 특징으로 하는 망상적혈구염색용시약.
- 식(I)(식중 R1은 수소원자, 또는 저급알킬기; R2및 R3은 수소원자 저급알킬기, 또는 저급알콕시기; R4는 수소원자, 아실기, 또는 저급알킬기; R5는 수소원자, 치환되어 있어도 되는 저급알킬기; Z는 유황원자, 산소원자, 또는 저급알킬기로 치환된 탄소원자; n은 1 또는 2; X-는 음이온이다)로 표시되는 화합물을 포함하는 시약을 사용하는 것을 특징으로 하는 망상적혈구측정방법.
- 제11항에 있어서, (i) 식 (I)로 표시되는 화합물과 적혈구의 비특이염색을 억제하기 위한 다가음이온과 완충제로 된 망상적혈구염색용시약과 혈액학적시료와를 혼합하므로서 측정시료를 조제하고 (ⅱ) 상기한 측정시료를 적색파장의 광원을 갖는 플로우사이트미터의 유체계에 도입하고 (ⅲ) 시즈플로우중을 흐르는 혈액학적시료의 세포에 적색파장의 광을 조사하고 (ⅳ) 세포로부터 발하는 산란광과 형광을 측정하고 (ⅴ) 산란광강도 혹은 산란광강도와 형광강도에 의해 소혈판과 적혈구계세포와를 판별하고 (ⅵ) 형광강도 혹은 형광강도와 산란광강도에 의해 적혈구, 망상적혈구 및 백혈구를 판별하고 (ⅶ) 혈소판, 적혈구, 망상적혈구 및 백혈구를 분류계수해서 세포수, 및 세포비율을 산출하는 것을 특징으로 하는 망상적혈구측정방법.
- 제12항에 있어서, 적색파장의 광원이 He-Ne 레이저 또는 적색반도체레이저인 것을 특징으로 하는 망상적혈구측정방법.
- 제12항에 있어서, 적어도 하나의 특정범위의 형광강도를 갖는 망상적혈구의 전체망상적혈구가 점하는 비율을 구하는 것으로 이루어진 것을 특징으로 하는 망상적혈구의 측정방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
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| JP95-260346 | 1995-10-06 | ||
| JP26034695A JP3425830B2 (ja) | 1995-10-06 | 1995-10-06 | 新規化合物とその用途 |
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| EP (1) | EP0767382B1 (ko) |
| JP (1) | JP3425830B2 (ko) |
| KR (1) | KR100463129B1 (ko) |
| CN (1) | CN1083839C (ko) |
| DE (1) | DE69628257T2 (ko) |
| TW (1) | TW438795B (ko) |
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| JP2001238216A (ja) * | 2000-02-23 | 2001-08-31 | Matsushita Electric Ind Co Ltd | 動画像圧縮装置および動画像圧縮方法 |
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| US7405082B2 (en) * | 2002-09-10 | 2008-07-29 | Sysmex Corporation | Methods and devices for measuring reticulocytes |
| JP4484442B2 (ja) | 2003-04-10 | 2010-06-16 | シスメックス株式会社 | 細菌測定方法と装置とプログラム |
| JP2005024472A (ja) * | 2003-07-04 | 2005-01-27 | Sysmex Corp | 幼若血小板測定装置 |
| JP4417143B2 (ja) * | 2004-03-11 | 2010-02-17 | シスメックス株式会社 | 試料分析装置、プログラムおよびそのプログラムを記録した記録媒体 |
| JP4806330B2 (ja) * | 2006-10-30 | 2011-11-02 | シスメックス株式会社 | 網状赤血球及び血小板測定用試薬、網状赤血球及び血小板測定用試薬キット並びに網状赤血球及び血小板測定方法 |
| JP4794414B2 (ja) * | 2006-10-30 | 2011-10-19 | シスメックス株式会社 | 血小板測定用試薬、血小板測定用試薬キット並びに血小板測定方法 |
| CN101231243B (zh) * | 2007-01-24 | 2011-11-16 | 深圳迈瑞生物医疗电子股份有限公司 | 网织红细胞检测试剂及检测方法 |
| CN101236158B (zh) * | 2007-01-29 | 2011-11-16 | 深圳迈瑞生物医疗电子股份有限公司 | 网织红细胞检测方法及检测装置 |
| CN101349644B (zh) | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
| CN105440725A (zh) | 2008-01-04 | 2016-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料，组合物及在生物样品染色中的用途 |
| BRPI0908250B1 (pt) * | 2008-02-18 | 2021-11-09 | Sysmex Corporation | Conjunto de reagentes para medição de plaquetas sanguíneas e seus métodos |
| CN101602762B (zh) | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
| CN101726579B (zh) | 2008-10-17 | 2014-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液检测试剂和方法 |
| US8367358B2 (en) | 2008-12-17 | 2013-02-05 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Reagent, kit and method for differentiating and counting leukocytes |
| JP5355145B2 (ja) * | 2009-02-26 | 2013-11-27 | シスメックス株式会社 | 試薬調製装置、検体測定装置および試薬調製方法 |
| CN101988082B (zh) | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
| JP5773644B2 (ja) * | 2010-12-28 | 2015-09-02 | 日揮触媒化成株式会社 | 水素化処理触媒の再生方法 |
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| CN104749144A (zh) * | 2013-12-31 | 2015-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | 血细胞检测试剂及血细胞处理方法和识别方法 |
| WO2016106688A1 (zh) * | 2014-12-31 | 2016-07-07 | 深圳迈瑞生物医疗电子股份有限公司 | 一种有核红细胞报警方法、装置及流式细胞分析仪 |
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- 1996-09-21 TW TW085111590A patent/TW438795B/zh not_active IP Right Cessation
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- 1996-10-04 EP EP96610036A patent/EP0767382B1/en not_active Expired - Lifetime
- 1996-10-04 CN CN96122614A patent/CN1083839C/zh not_active Expired - Lifetime
- 1996-10-04 KR KR1019960043840A patent/KR100463129B1/ko not_active IP Right Cessation
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Also Published As
| Publication number | Publication date |
|---|---|
| EP0767382B1 (en) | 2003-05-21 |
| US5821127A (en) | 1998-10-13 |
| DE69628257D1 (de) | 2003-06-26 |
| EP0767382A2 (en) | 1997-04-09 |
| EP0767382A3 (en) | 1998-02-25 |
| CN1154966A (zh) | 1997-07-23 |
| DE69628257T2 (de) | 2004-01-29 |
| JPH09104683A (ja) | 1997-04-22 |
| TW438795B (en) | 2001-06-07 |
| JP3425830B2 (ja) | 2003-07-14 |
| KR100463129B1 (ko) | 2005-07-01 |
| CN1083839C (zh) | 2002-05-01 |
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