KR960008309A - 백혈구 분류방법 - Google Patents

백혈구 분류방법 Download PDF

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KR960008309A
KR960008309A KR1019950022191A KR19950022191A KR960008309A KR 960008309 A KR960008309 A KR 960008309A KR 1019950022191 A KR1019950022191 A KR 1019950022191A KR 19950022191 A KR19950022191 A KR 19950022191A KR 960008309 A KR960008309 A KR 960008309A
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leukocytes
leukocyte
classification method
angle
scattered light
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가오루 다까라다
지히로 고우쯔끼
요시히로 효사
다까시 사까다
야스마사 아까이
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하시모도 레이죠
도아 이요우 덴시 가부시끼가이샤
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    • G01—MEASURING; TESTING
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    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
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Abstract

1. 청구범위에 기재된 발명이 속한 기술분야
본 발명은 백혈구 분류방법에 관한 것이다.
2. 발명이 해결하려고 하는 기술적 과제
본 발명은 값싸고 간단한 구성의 장치로 백혈구를 적어도 5분류하는 방법을 제공하는 것을 목적으로 하고 있다.
3. 발명의 해결방법의 요지
(ⅰ), (a)적혈구를 용해시킬 수 있으나 백혈구의 세포막의 일부에 손상을 주는데 충분한 양의 적어도 1종류의 이온성계면활성제와, (b)백혈구내의 양이온 하전성분과 결합해서 백혈구간에 형태적 차이를 부여하는데 충분한 양의 적어도 1종류의 음이온기를 갖는 유기화합물과, (c)비이온계면활성제와, (d)pH조정용의 완충제로된 백혈구 4분류용 시약을 전체혈액시료의 일부에 첨가하고, 세포의 크기정보와 형태정보를 측정해서 백혈구를 적어도 림프구, 단구, 및 호산구에 각각 대응하는 3집단과 호중구 및 호염기구에 대응하는 1집단과의 4집단으로 분류계수하고, (ⅱ)호염기구 측정용 시약을 전체혈액시료의 다른 일부에 첨가하여 적어도 세포의 크기정보를 측정해서 호염기구를 분류계수하고, (ⅲ)각각 얻어진 계수치에 기초해서 백혈구를 적어도 5분류하는 것을 특징으로 하는 백혈구 분류방법.
4. 발명의 중요한 용도
본 발명은 다른 시약에 의해 처리한 백혈구를 따로따로 측정하므로서 백혈구를 5분류하는 백혈구 분류방법에 관한 것이다.

Description

백혈구 분류방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제3도는 본 발명의 백혈구 분류방법에 있어서 아주 적당히 사용되는 측정장치의 구성을 나타내는 개략도.
제4도는 제3도의 측정장치에 사용되는 포트다이오드를 나타내는 사시도.
제5도는 포트다이오드의 수광센서의 배치를 나타내는 개략도.

Claims (9)

  1. (ⅰ)(a)적혈구를 용해시킬 수 있으나 백혈구의 세포막의 일부에 손상을 주는데 충분한 양의 적어도 1종류의 이온성계면활성제와, (b)백혈구내의 양이온 하전성분과 결합해서 백혈구간에 헝태적 차이를 부여하는데 충분한 양의 적어도 1종류의 음이온기를 갖는 유기화합물과, (c)비이온계면활성제와, (d)pH조정용의 완충제로된 백혈구 4분류용 시약을 전체혈액시료의 일부에 첨가하고, 세포의 크기정보와 형태정보를 측정해서 백혈구를 적어도 림프구(Lym), 단구(Mono), 및 호산구(Eo)에 각각 대응하는 3집단과 호중구 및 호염기구(GRAN)에 대응하는 1집단과의 4집단으로 분류계수하고, (ⅱ)호염기구 측정용 시약을 전체혈액시료의 다른 일부에 첨가하여 적어도 세포의 크기정보를 측정해서 호염기구(Baso)를 분류계수하고, (ⅲ)각각 얻어진 계수치에 기초해서 백혈구를 적어도 5분류하는 것을 특징으르 하는 백혈구 분류방법.
  2. 제1항에 있어서, 세포의 크기정보를 측정하는 공정이 저각산란광을 측정하는 것을 특징으로 하는 백혈구 분류방법.
  3. 제2항에 있어서, 저각산란광의 측정각도가 1∼5°인 것을 특징으로 하는 백혈구 분류방법.
  4. 제1항에 있어서, 형태정보를 측정하는 공정이 광각산란광을 측정하는 것을 특징으로 하는 백혈구 분류방법.
  5. 제4항에 있어서, 광각산란광의 측정각도가 6-20°인 것을 특징으로 하는 백혈구 분류방법.
  6. 제1항에 있어서, 세포의 크기정보와 형태정보를 측정하는 공정이 저각과 광각의 2가지의 산란광을 측정하는 것을 특징으로 하는 백혈구 분류방법.
  7. 제6항에 있어서, 측정하는 저각과 광각의 2가지의 산란광의 각도가 1∼5°및 6∼20°인 것을 특징으로 하는 백혈구 분류방법.
  8. 제6항 또는 제7항에 있어서, 2가지의 각도의 산란광의 검출을 1개의 포트다이오드(PD)센서로 행하는 것을 특징으로 히는 백현구 분류방법.
  9. 제1항에 있어서, 공정(ⅰ)의 적어도 4집단의 분류계수와 공정(ⅱ)의 호염기구(Baso)의 분류계수를 동일한 검출기를 사용해서 행하는 것을 특징으로 하는 백혈구 분류방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950022191A 1994-08-03 1995-07-26 백혈구분류방법 KR100354048B1 (ko)

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JP18263394A JP3355038B2 (ja) 1994-08-03 1994-08-03 白血球分類方法
JP94-182633 1994-08-03

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KR960008309A true KR960008309A (ko) 1996-03-22
KR100354048B1 KR100354048B1 (ko) 2003-01-29

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US (1) US5677183A (ko)
EP (1) EP0695936B1 (ko)
JP (1) JP3355038B2 (ko)
KR (1) KR100354048B1 (ko)
CN (1) CN1113241C (ko)
AU (1) AU695767B2 (ko)
CA (1) CA2151667A1 (ko)
DE (1) DE69528022T2 (ko)
TW (1) TW325521B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
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KR20210000215A (ko) 2019-06-24 2021-01-04 국민대학교산학협력단 혈액영상을 이용한 백혈구 감별방법

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843608A (en) * 1995-06-08 1998-12-01 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
US5786224A (en) * 1995-06-29 1998-07-28 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
JP4042925B2 (ja) * 1996-11-20 2008-02-06 シスメックス株式会社 幼若白血球の分類計数法
US5830701A (en) * 1997-03-28 1998-11-03 Tao Medical Electronics Co., Ltd. Method of detecting hematopoietic progenitor cells
WO1999024831A1 (fr) * 1997-11-11 1999-05-20 Kowa Company, Ltd. Procede et dispositif de numeration de globules blancs
US5952192A (en) * 1998-01-07 1999-09-14 Western Michigan University Method of fluorescent analysis of biological sample utilizing biebrich scarlet
FR2791138B1 (fr) * 1999-03-19 2001-04-27 Abx Sa Reactif pour la determination des leucocytes et la mesure de l'hemoglobine dans un echantillon de sang
US6214625B1 (en) 1999-04-28 2001-04-10 Coulter International Corp. Composition and method for differentiation of basophils and eosinophils in blood
US6210969B1 (en) 1999-04-28 2001-04-03 Coulter International Corp. Composition and method for differentiation of basophil and eosinophil subpopulations of leukocytes in blood
US6485923B1 (en) * 2000-02-02 2002-11-26 Lifescan, Inc. Reagent test strip for analyte determination having hemolyzing agent
DE60218074T2 (de) * 2001-03-29 2008-02-07 Sysmex Corp. Durchflusszytometer
US6979570B2 (en) * 2001-07-26 2005-12-27 Sysmex Corporation Particle analyzer and particle analyzing method
JP3600231B1 (ja) * 2003-09-30 2004-12-15 森永製菓株式会社 イムノアッセイ
US7580120B2 (en) * 2005-04-07 2009-08-25 Sysmex Corporation Blood analyzer, sample analyzer, and flow cytometer
JP4964446B2 (ja) * 2005-09-14 2012-06-27 シスメックス株式会社 分析装置及び検体情報処理プログラム
CN101078720B (zh) * 2006-05-22 2010-12-01 深圳迈瑞生物医疗电子股份有限公司 一种改进的用于对白细胞进行分类的试剂及方法
CN101078721B (zh) * 2006-05-23 2010-12-22 深圳迈瑞生物医疗电子股份有限公司 对白细胞进行分类的试剂及方法
JP4796443B2 (ja) * 2006-06-08 2011-10-19 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
JP4914656B2 (ja) * 2006-06-26 2012-04-11 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
CN101236192B (zh) * 2007-01-29 2015-05-13 深圳迈瑞生物医疗电子股份有限公司 溶血剂、白细胞分类试剂体系及分类方法
CN101349644B (zh) 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
ES2490865T3 (es) * 2007-09-27 2014-09-04 Sysmex Corporation Kit de reactivos para análisis de muestras y método de análisis de muestras
CN101424614B (zh) * 2007-10-29 2011-04-13 深圳迈瑞生物医疗电子股份有限公司 嗜碱性粒细胞分析试剂及测定方法
CN101464454B (zh) * 2007-12-18 2016-08-03 深圳迈瑞生物医疗电子股份有限公司 白细胞分类试剂及方法
CN101470108B (zh) * 2007-12-24 2013-11-27 深圳迈瑞生物医疗电子股份有限公司 一种对白细胞进行分类的试剂和方法
CN101475754A (zh) 2008-01-04 2009-07-08 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
EP2280278A4 (en) * 2008-05-09 2015-04-08 Sysmex Corp BLOOD ANALYSIS DEVICE, BLOOD ANALYSIS METHOD, AND HEMOLYTIC AGENT
CN101602762B (zh) 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
CN101726579B (zh) 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 血液检测试剂和方法
US8842900B2 (en) * 2008-10-28 2014-09-23 Sysmex Corporation Specimen processing system and blood cell image classifying apparatus
JP5557994B2 (ja) * 2008-10-28 2014-07-23 シスメックス株式会社 検体処理システムおよび血球画像分類装置
CN101723874B (zh) * 2008-10-31 2013-09-11 深圳迈瑞生物医疗电子股份有限公司 花菁类化合物及其在生物样品染色中的用途
CN101750476B (zh) * 2008-12-08 2015-06-03 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
CN101750274B (zh) 2008-12-17 2014-06-25 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法
JP5452058B2 (ja) * 2009-03-31 2014-03-26 シスメックス株式会社 血液分析装置
CN101988082B (zh) 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
CN102115456B (zh) * 2009-12-30 2014-08-20 深圳迈瑞生物医疗电子股份有限公司 花菁类化合物，包含所述化合物的组合物及其在细胞检测中的用途
US9658215B2 (en) * 2011-04-07 2017-05-23 Beckman Coulter, Inc. Identifying and enumerating early granulated cells (EGCs)
EP2520926B1 (en) * 2011-05-05 2022-06-15 Sysmex Corporation Blood analyzer, blood analysis method, and computer program product
US8445284B2 (en) 2011-06-17 2013-05-21 Constitution Medical, Inc. Fixative and staining solutions
KR20180023061A (ko) * 2014-02-28 2018-03-06 시스멕스 가부시키가이샤 소변 시료 분석 방법, 소변 시료 분석용 시약 및 소변 시료 분석용 시약 키트
CN109991052A (zh) * 2019-03-12 2019-07-09 迪瑞医疗科技股份有限公司 一种用于白细胞、嗜碱性粒细胞计数和血红蛋白测定的溶血剂
CN110031383A (zh) * 2019-04-22 2019-07-19 深圳开立生物医疗科技股份有限公司 一种白细胞分类试剂及方法
CN111006990A (zh) * 2019-12-31 2020-04-14 山东博科生物产业有限公司 血细胞分析用试剂及其应用
CN114047109B (zh) * 2022-01-11 2022-06-21 深圳市帝迈生物技术有限公司 一种样本分析仪及其计数方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1255197A (en) 1984-09-24 1989-06-06 Joseph L. Orlik Leukocyte differentiation composition and method
JPH06100596B2 (ja) * 1986-09-10 1994-12-12 東亜医用電子株式会社 フロ−サイトメトリ−による白血球の分類方法
KR970007077B1 (ko) * 1987-03-13 1997-05-02 코울터 일렉트로닉스 인커퍼레이티드 광산란 기술을 이용한 다중-부분식별 분석 방법
US5155044A (en) * 1987-03-13 1992-10-13 Coulter Electronics, Inc. Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
US5389549A (en) * 1987-05-29 1995-02-14 Toa Medical Electronics Co., Ltd. Method for classifying leukocytes and a reagent used therefor
JP2619900B2 (ja) * 1988-01-27 1997-06-11 東亜医用電子株式会社 血液中の白血球およびヘモグロビンの測定用試薬および方法
CA2016699C (en) * 1989-05-15 2003-11-18 Paul N. Marshall Lytic agents and uses thereof
JP2778745B2 (ja) * 1989-06-19 1998-07-23 東亜医用電子株式会社 血液中の白血球弁別測定用試薬
JP2836865B2 (ja) * 1989-10-23 1998-12-14 東亜医用電子株式会社 血液中の白血球およびヘモグロビンの測定用試薬
FR2654744B1 (fr) * 1989-11-20 1992-03-13 Abx Sa Reactif et methode d'utilisation de celui-ci pour la determination automatique en cytometrie de flux d'au moins une sous-population leucocytaire a partir du sang total.
US5242832A (en) * 1990-03-01 1993-09-07 Toa Medical Electronics Co., Ltd. Reagent for measurement of leukocytes and hemoglobin in blood
US5316725A (en) * 1991-06-07 1994-05-31 Edward Lawrence Carver, Jr. Reagent system for the improved determination of white blood cell subpopulations
EP0598663B1 (en) * 1992-11-19 2000-02-02 Sysmex Corporation A pretreatment method for blood analysis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210000215A (ko) 2019-06-24 2021-01-04 국민대학교산학협력단 혈액영상을 이용한 백혈구 감별방법

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CA2151667A1 (en) 1996-02-04
DE69528022D1 (de) 2002-10-10
AU695767B2 (en) 1998-08-20
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