KR950703065A - 핵산 검출시에 허위 음성 시험 결과를 제거하는 방법(elimination of false negatives in nucleic acid detection) - Google Patents

핵산 검출시에 허위 음성 시험 결과를 제거하는 방법(elimination of false negatives in nucleic acid detection) Download PDF

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KR950703065A
KR950703065A KR1019950700692A KR19950700692A KR950703065A KR 950703065 A KR950703065 A KR 950703065A KR 1019950700692 A KR1019950700692 A KR 1019950700692A KR 19950700692 A KR19950700692 A KR 19950700692A KR 950703065 A KR950703065 A KR 950703065A
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nucleic acid
internal control
acid analyte
analyte
amplified
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KR1019950700692A
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KR100292925B1 (ko
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팀 키비츠
페터 프랭클린 렌스
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에프.쥐.엠.헤르만스, 피.케이.브론스
악조 노벨 엔.브이. (Akzo Nobel N.V.)
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract

본 발명은 샘플내 증폭된 핵산 피분석물을 검출하기 위한 분석에서 허위음성 결과를 제거하는 방법에 관한 것이다. 본 방법에서는 증폭시키기 전에 내부 대조군을 샘플에 첨가하는데, 이 내부 대조군은 핵산 피분석물과 동일한 증폭 시약으로 증폭될 수 있고 핵산 피분석물과 구별될 수 있는 핵산을 포함한다. 내부 대조군은 핵산 피분석물과 구별되도록 돌연변이된, 핵산 피분석물에 상응하는 핵산 서열을 포함하는 것이 바람직하다.

Description

핵산 검출시에 허위 음성 시험 결과를 제거하는 방법(ELIMINATION OF FALSE NEGATIVES IN NUCLEIC ACID DETECTION)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 샘플내 증폭된 핵산 피분석물을 검출하기 위한 분석에서 허위 음성(false negative)결과를 제거하는 방법으로서, 증폭시키기 전에, 핵산 피분석물과 동일한 증폭 시약으로 증폭될 수 있고 핵산 피분석물과 구별될 수 있는 핵산을 포함하는 내부 대조군(internal control)을 첨부하는 것을 특징으로 하는 방법.
  2. 제1항에 있어서, 내부 대조군이 핵산 피분석물과 구별되도록 돌연변이된, 핵산 피분석물에 상응하는 핵산 서열을 포함하는 것을 특징으로 하는 방법.
  3. 제1항 또는 제2항에 있어서, 내부 대조군이 핵산 피분석물보다 더 적은 효율로 증폭되는 것을 특징으로 하는 방법.
  4. 제1항 내지 제3항중 어느 한 항에 있어서, 내부 대조군 및 핵산 피분석물이 둘다 하나의 올리고뉴클레오티드 및 동일한 상보적 올리고뉴클레오티드와 검출 가능한 복합체를 형성할 수 있는 것을 특징으로 하는 방법.
  5. 제4항에 있어서, 핵산 피분석물 및 내부 대조군이 길이가 상이하며, 둘다 동일한 상보적 올리고뉴클레오티드와 검출 가능한 복합체를 형성할 수 있는 것을 특징으로 하는 방법.
  6. 제4항에 있어서, 상보적 올리고뉴클레오티드가 고체상상에 고정화되며, 검출 가능한 복합체가 각각 상이하게 표지한 반응물과 반응할 수 있는 것을 특징으로 하는 방법.
  7. 제1항 내지 제3항중 어느 한 항에 있어서, 핵산 피분석물 및 내부 대조군 핵산이 각각 상이한 상보적 올리고 뉴클레오티드와 검출가능한 복합체를 형성할 수 있는 것을 특징으로 하는 방법.
  8. 제7항에 있어서, 상기 올리고뉴클레오티드가 각각 고체상상의 상이한 지점에 고정화된 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950700692A 1992-08-24 1993-08-20 핵산검출기에허위음성시험결과를제거하는방법 KR100292925B1 (ko)

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NL92202563.0 1992-08-24
EP92202563.0 1992-08-24
EP92202563 1992-08-24
PCT/EP1993/002248 WO1994004706A1 (en) 1992-08-24 1993-08-20 Elimination of false negatives in nucleic acid detection

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US (1) US5770360A (ko)
EP (1) EP0656955B1 (ko)
JP (1) JP3560974B2 (ko)
KR (1) KR100292925B1 (ko)
AT (1) ATE162226T1 (ko)
AU (1) AU685396B2 (ko)
CA (1) CA2143202C (ko)
DE (1) DE69316372T2 (ko)
DK (1) DK0656955T3 (ko)
ES (1) ES2114066T3 (ko)
FI (1) FI111739B (ko)
GR (1) GR3026631T3 (ko)
WO (1) WO1994004706A1 (ko)
ZA (1) ZA936015B (ko)

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ES2114066T3 (es) 1998-05-16
DK0656955T3 (da) 1998-09-14
GR3026631T3 (en) 1998-07-31
CA2143202A1 (en) 1994-03-03
ATE162226T1 (de) 1998-01-15
ZA936015B (en) 1994-03-10
FI950836A (fi) 1995-02-23
US5770360A (en) 1998-06-23
FI111739B (fi) 2003-09-15
EP0656955B1 (en) 1998-01-14
JP3560974B2 (ja) 2004-09-02
JPH08500732A (ja) 1996-01-30
EP0656955A1 (en) 1995-06-14
DE69316372T2 (de) 1998-08-27
DE69316372D1 (de) 1998-02-19
WO1994004706A1 (en) 1994-03-03
CA2143202C (en) 2007-06-12
FI950836A0 (fi) 1995-02-23
KR100292925B1 (ko) 2006-03-31
AU4951393A (en) 1994-03-15
AU685396B2 (en) 1998-01-22

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