DE60109733D1 - Verfahren zur immobilisierung von affinen reagentien an hydrophoben festphasen - Google Patents
Verfahren zur immobilisierung von affinen reagentien an hydrophoben festphasenInfo
- Publication number
- DE60109733D1 DE60109733D1 DE60109733T DE60109733T DE60109733D1 DE 60109733 D1 DE60109733 D1 DE 60109733D1 DE 60109733 T DE60109733 T DE 60109733T DE 60109733 T DE60109733 T DE 60109733T DE 60109733 D1 DE60109733 D1 DE 60109733D1
- Authority
- DE
- Germany
- Prior art keywords
- solid phase
- immobilizing
- reagents
- hydrophobic solid
- affine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007790 solid phase Substances 0.000 title abstract 6
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 5
- 230000002209 hydrophobic effect Effects 0.000 title abstract 3
- 230000003100 immobilizing effect Effects 0.000 title abstract 3
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 title 1
- 230000004913 activation Effects 0.000 abstract 2
- 230000008878 coupling Effects 0.000 abstract 2
- 238000010168 coupling process Methods 0.000 abstract 2
- 238000005859 coupling reaction Methods 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 239000012491 analyte Substances 0.000 abstract 1
- 238000004166 bioassay Methods 0.000 abstract 1
- 150000001718 carbodiimides Chemical class 0.000 abstract 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000007824 enzymatic assay Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0000376 | 2000-01-13 | ||
| FR0000376A FR2803913B1 (fr) | 2000-01-13 | 2000-01-13 | Procede d'immobilisation de reactif(s) affin(s) sur phase solide hydrophobe |
| PCT/FR2001/000095 WO2001051927A1 (fr) | 2000-01-13 | 2001-01-12 | Procede d'immobilisation de reactif(s) affin(s) sur phase solide hydrophobe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60109733D1 true DE60109733D1 (de) | 2005-05-04 |
| DE60109733T2 DE60109733T2 (de) | 2006-02-09 |
Family
ID=8845855
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60109733T Expired - Lifetime DE60109733T2 (de) | 2000-01-13 | 2001-01-12 | Verfahren zur immobilisierung von affinen reagentien an hydrophoben festphasen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7052887B2 (de) |
| EP (1) | EP1247096B1 (de) |
| JP (1) | JP4746810B2 (de) |
| AT (1) | ATE292283T1 (de) |
| AU (1) | AU777713B2 (de) |
| CA (1) | CA2397500C (de) |
| DE (1) | DE60109733T2 (de) |
| ES (1) | ES2239121T3 (de) |
| FR (1) | FR2803913B1 (de) |
| WO (1) | WO2001051927A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1310793A4 (de) * | 2000-08-08 | 2005-11-16 | Toyo Kohan Co Ltd | Substrataktivierungskit und verfahren zum nachweis von dna oder so ähnlich |
| EP1343010A1 (de) * | 2002-03-04 | 2003-09-10 | Glaucus Proteomics B.V. | Matrix zur Immobilisierung von biologischen Substanzen, sowie Verfahren zur Herstellung und Verwending derartige Matrixen |
| WO2003075012A1 (en) * | 2002-03-04 | 2003-09-12 | Steinberg Holding B.V. | Matrix for coupling of a biological molecule, method for producing and use of said matrix |
| KR100601983B1 (ko) * | 2005-01-20 | 2006-07-18 | 삼성전자주식회사 | 시료 내 핵산 증폭 저해 물질의 제거 방법 |
| WO2016073228A1 (en) * | 2014-11-03 | 2016-05-12 | Merck Patent Gmbh | Soluble intein fusion proteins and methods for purifying biomolecules |
| CN111060686A (zh) * | 2019-11-22 | 2020-04-24 | 苏州普瑞斯生物科技有限公司 | 一种纤溶酶原激活物的检测试剂盒及制备方法 |
| CN112881680B (zh) * | 2021-01-19 | 2022-11-15 | 深圳市卓润生物科技有限公司 | 连接有dsDNA的磁性微粒的制备方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| FI63596C (fi) | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| JP2641880B2 (ja) | 1986-08-11 | 1997-08-20 | シスカ・ダイアグノスティックス・インコーポレーテッド | 核酸プローブアッセイ法および組成物 |
| EP0446260B1 (de) | 1988-11-21 | 1994-03-02 | Dynal A.S. | Sonden aus nukleinsäuren |
| DE69032383T2 (de) * | 1989-08-04 | 1998-12-10 | Behringwerke Ag | Heterogene Bindungstests |
| US5232829A (en) | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
| US6299881B1 (en) * | 1997-03-24 | 2001-10-09 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Uronium salts for activating hydroxyls, carboxyls, and polysaccharides, and conjugate vaccines, immunogens, and other useful immunological reagents produced using uronium salts |
-
2000
- 2000-01-13 FR FR0000376A patent/FR2803913B1/fr not_active Expired - Fee Related
-
2001
- 2001-01-12 DE DE60109733T patent/DE60109733T2/de not_active Expired - Lifetime
- 2001-01-12 AU AU31885/01A patent/AU777713B2/en not_active Ceased
- 2001-01-12 WO PCT/FR2001/000095 patent/WO2001051927A1/fr active IP Right Grant
- 2001-01-12 CA CA2397500A patent/CA2397500C/en not_active Expired - Fee Related
- 2001-01-12 EP EP01903931A patent/EP1247096B1/de not_active Expired - Lifetime
- 2001-01-12 US US10/181,090 patent/US7052887B2/en not_active Expired - Lifetime
- 2001-01-12 JP JP2001552089A patent/JP4746810B2/ja not_active Expired - Lifetime
- 2001-01-12 ES ES01903931T patent/ES2239121T3/es not_active Expired - Lifetime
- 2001-01-12 AT AT01903931T patent/ATE292283T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE292283T1 (de) | 2005-04-15 |
| CA2397500C (en) | 2011-08-02 |
| US20040052797A1 (en) | 2004-03-18 |
| EP1247096B1 (de) | 2005-03-30 |
| DE60109733T2 (de) | 2006-02-09 |
| AU3188501A (en) | 2001-07-24 |
| ES2239121T3 (es) | 2005-09-16 |
| CA2397500A1 (en) | 2001-07-19 |
| US7052887B2 (en) | 2006-05-30 |
| WO2001051927A1 (fr) | 2001-07-19 |
| FR2803913B1 (fr) | 2002-08-16 |
| FR2803913A1 (fr) | 2001-07-20 |
| EP1247096A1 (de) | 2002-10-09 |
| JP2003523503A (ja) | 2003-08-05 |
| AU777713B2 (en) | 2004-10-28 |
| JP4746810B2 (ja) | 2011-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR950703065A (ko) | 핵산 검출시에 허위 음성 시험 결과를 제거하는 방법(elimination of false negatives in nucleic acid detection) | |
| ATE322558T1 (de) | Sensible und multiplexe diagnostische tests zur proteinanalyse | |
| ATE549414T1 (de) | Verfahren zur isolierung von zellen | |
| ATE281648T1 (de) | Verfahren zum assay von troponin i und t und komplexen von troponin i und t und auswahl von antikörpern zur verwendung in immunoassays | |
| AU582293B2 (en) | Heterogeneous specific binding assay employing an aggregatable binding reagent | |
| DE69521104T2 (de) | Spezifische Bindungstests und Reagenzien dafür | |
| CY1107621T1 (el) | Μεθοδος ανιχνευσης αντισωματων σε ενα δειγμα | |
| ATE360708T1 (de) | Test-streife-probesystem und verfahren zur nachweis von spezifischen nukleinsäuresequenzen | |
| DE60109733D1 (de) | Verfahren zur immobilisierung von affinen reagentien an hydrophoben festphasen | |
| ATE319735T1 (de) | Aufreinigung von substanzen aus einer biologischen probe | |
| ATE470148T1 (de) | Verfahren zum vorbehandeln einer probe | |
| DE69924128D1 (de) | Verfahren zur erkennung von verbindungen,die die wechselwirkung von rna und rna-bindungsproteinen beinflussen | |
| DE69935850D1 (de) | Verfahren zur messung der gesamkonzentration eines analytes | |
| DE60012723D1 (de) | Verfahren zur bestimmung von disaccharidasen | |
| ES2179214T3 (es) | Metodos de captura y procedimientos de ensayos. | |
| WO2002064823A3 (de) | Verfahren zum nachweis und/oder zur quantifizierung eines analyten | |
| NO20012304L (no) | Testsett for fosfatase-målsökende toksiner | |
| WO2003048388A3 (en) | Immuno polymerase chain reaction assay | |
| HUT67677A (en) | Label trapping assay - nonseparation binding assay method | |
| FR2840687B1 (fr) | Procede de detection simultanee de reactions d'hybridation et immunologiques et ses utilisations en diagnostic | |
| ATE201453T1 (de) | Modulation von bindungseigenschaften der nukleinsäure bindungspartner | |
| DE69920040D1 (de) | Verfahren, reagentiensätze und zusammenstellungen zur erkennung von elektrostatisch an matrizen gebundene nukleinsäuren |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| R082 | Change of representative |
Ref document number: 1247096 Country of ref document: EP Representative=s name: BEETZ & PARTNER PATENT- UND RECHTSANWAELTE, 80538 |