WO2002064823A3 - Verfahren zum nachweis und/oder zur quantifizierung eines analyten - Google Patents

Verfahren zum nachweis und/oder zur quantifizierung eines analyten Download PDF

Info

Publication number
WO2002064823A3
WO2002064823A3 PCT/EP2002/001324 EP0201324W WO02064823A3 WO 2002064823 A3 WO2002064823 A3 WO 2002064823A3 EP 0201324 W EP0201324 W EP 0201324W WO 02064823 A3 WO02064823 A3 WO 02064823A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
microparticles
probe
quantifying
detecting
Prior art date
Application number
PCT/EP2002/001324
Other languages
English (en)
French (fr)
Other versions
WO2002064823A2 (de
Inventor
Emil Palecek
Joerg Hassmann
Original Assignee
November Ag Molekulare Medizin
Emil Palecek
Joerg Hassmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November Ag Molekulare Medizin, Emil Palecek, Joerg Hassmann filed Critical November Ag Molekulare Medizin
Priority to US10/467,884 priority Critical patent/US20040096859A1/en
Priority to DE10290517T priority patent/DE10290517D2/de
Publication of WO2002064823A2 publication Critical patent/WO2002064823A2/de
Publication of WO2002064823A3 publication Critical patent/WO2002064823A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum Nachweis und/oder Quantifizierung eines Analyten in einer Flüssigkeit, insbesondere von Nukleinsäuren, mit folgenden Schritten:a) Bereitstellen erster Mikropartikel und einer Sonde, welche eine spezifische Affinität für den Analyten und für die ersten Mikropartikel aufweist, oder von zweiten Mikropartikeln mit der an deren Oberfläche gebunden Sonde,b) Herstellen einer den Analyten, die Sonde und die ersten Mikropartikel enthaltenden ersten Lösung unter Bedingungen, unter denen die Sonde an den Analyten und die ersten Mikropartikel bindet oder einer den Analyten und die zweiten Mikropartikel enthaltenden ersten Lösung unter Bedingungen, unter denen der Analyt an die Sonde bindet, c) Trennen der ersten oder zweiten Mikropartikel von der ersten Lösung,d) Nachweis des Analyten mittels eines elektrochemischen Verfahrens, wobei die ersten oder zweiten Mikropartikel zum Nachweis des Analyten in eine zweite Lösung überführt werden.
PCT/EP2002/001324 2001-02-12 2002-02-08 Verfahren zum nachweis und/oder zur quantifizierung eines analyten WO2002064823A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/467,884 US20040096859A1 (en) 2001-02-12 2002-02-08 Method for detecting and/or quantifying an analyte
DE10290517T DE10290517D2 (de) 2001-02-12 2002-02-08 Verfahren zum Nachweis und/oder zur Quantifizierung eines Analyten

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10106654A DE10106654A1 (de) 2001-02-12 2001-02-12 Verfahren zum Nachweis und/oder zur Quantifizierung eines Analyten
DE10106654.6 2001-02-12

Publications (2)

Publication Number Publication Date
WO2002064823A2 WO2002064823A2 (de) 2002-08-22
WO2002064823A3 true WO2002064823A3 (de) 2003-04-17

Family

ID=7673892

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/001324 WO2002064823A2 (de) 2001-02-12 2002-02-08 Verfahren zum nachweis und/oder zur quantifizierung eines analyten

Country Status (3)

Country Link
US (1) US20040096859A1 (de)
DE (2) DE10106654A1 (de)
WO (1) WO2002064823A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003210410A1 (en) * 2002-03-08 2003-09-22 November Aktiengesellschaft Gesellschaft Fur Molekulare Medizin Method for identifying, quantifying and/or characterizing an analyte
DE102005039726B3 (de) 2005-08-19 2007-01-11 Universität Rostock Verfahren zur Markierung und Analyse von Nukleinsäuren
CN110702756A (zh) * 2019-09-02 2020-01-17 寅木医疗科技(杭州)有限公司 一种检测核酸的电化学方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999067425A2 (en) * 1998-06-23 1999-12-29 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes
US6078782A (en) * 1988-11-03 2000-06-20 Igen International Inc. Methods for improved particle electrochemiluminescence assays

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4216696C2 (de) * 1992-04-10 1995-01-26 Deutsche Aerospace Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5871918A (en) * 1996-06-20 1999-02-16 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
DE4430973A1 (de) * 1994-07-25 1996-02-01 Boehringer Mannheim Gmbh Hapten-markierte Peptide
US6346387B1 (en) * 1995-06-27 2002-02-12 Xanthon, Inc. Detection of binding reactions using labels detected by mediated catalytic electrochemistry
DE19602078A1 (de) * 1996-01-20 1997-07-24 Bst Bio Sensor Tech Gmbh Vorrichtung für amperometrische kompetitive homogene Enzymimmunotests
EP0827960A1 (de) * 1996-09-10 1998-03-11 Ajinomoto Co., Inc. Verfahren zur Herstellung von Purinderivaten
US6117631A (en) * 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
AU5301598A (en) * 1997-02-10 1998-08-13 Gist-Brocades B.V. Detection of analytes using electrochemistry
DE19730497C2 (de) * 1997-07-16 2000-02-10 Heermann Klaus Hinrich Verfahren zum Waschen, zur Separierung und Konzentrierung von Biomolekülen unter Verwendung eines Magnetstifts
DE19823719B4 (de) * 1998-05-27 2011-12-15 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zum Aufkonzentrieren von Substanzen
DE19828441A1 (de) * 1998-06-25 1999-12-30 Roche Diagnostics Gmbh Verwendung derivatisierter Reagenzien in Chemilumineszenz- und Elektrochemilumineszenz-Nachweisverfahren

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6078782A (en) * 1988-11-03 2000-06-20 Igen International Inc. Methods for improved particle electrochemiluminescence assays
WO1999067425A2 (en) * 1998-06-23 1999-12-29 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CHANEY R ET AL: "Direct detection of bacteria in cellular blood products using bacterial ribosomal RNA-directed probes coupled to electrochemiluminescence.", TRANSFUSION MEDICINE (OXFORD, ENGLAND) ENGLAND SEP 1999, vol. 9, no. 3, September 1999 (1999-09-01), pages 177 - 188, XP002228418, ISSN: 0958-7578 *
GEHRING A G ET AL: "Enzyme-linked immunomagnetic electrochemical detection of Salmonella typhimurium", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 195, no. 1, 9 September 1996 (1996-09-09), pages 15 - 25, XP004021249, ISSN: 0022-1759 *
HAUKANES B-I ET AL: "Application of magnetic beads in bioassays", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 11, no. 1, 1993, pages 60 - 63, XP000609039, ISSN: 0733-222X *
PALECEK E ET AL: "Determination of nanogram quantities of osmium-labeled nucleic acids by stripping (inverse) voltammetry", ANALYTICAL BIOCHEMISTRY, vol. 132, no. 2, 1983, pages 236 - 242, XP009004620, ISSN: 0003-2697 *
PALECEK E ET AL: "DNA hybridization at microbeads with cathodic stripping voltammetric detection.", TALANTA, vol. 56, no. 5, 1 April 2002 (2002-04-01), 1 April, 2002, pages 919 - 930, XP001122239, ISSN: 0039-9140 *
PALECEK E ET AL: "Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor.", ANALYTICA CHIMICA ACTA, vol. 469, no. 1, 26 September 2002 (2002-09-26), 26 September, 2002, pages 73 - 83, XP002228419, ISSN: 0003-2670 *
PALECEK E ET AL: "New approaches in the development of DNA sensors: hybridization and electrochemical detection of DNA and RNA at two different surfaces.", BIOELECTROCHEMISTRY (AMSTERDAM, NETHERLANDS) NETHERLANDS MAY 2002, vol. 56, no. 1-2, May 2002 (2002-05-01), pages 85 - 90, XP002228420, ISSN: 1567-5394 *
PALECEK E: "Reaction of nucleic acid bases with the mercury electrode: determination of purine derivatives at submicromolar concentrations by means of cathodic stripping voltammetry", ANALYTICAL BIOCHEMISTRY, vol. 108, no. 1, 1980, pages 129 - 138, XP009004389, ISSN: 0003-2697 *
YU H ET AL: "A comparative study of PCR product detection and quantitation by electrochemiluminescence and fluorescence", JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, WILEY AND SONS, CHICHESTER, GB, vol. 10, no. 4, 1995, pages 239 - 245, XP001010086, ISSN: 0884-3996 *

Also Published As

Publication number Publication date
US20040096859A1 (en) 2004-05-20
DE10290517D2 (de) 2003-12-11
DE10106654A1 (de) 2002-09-05
WO2002064823A2 (de) 2002-08-22

Similar Documents

Publication Publication Date Title
WO2006117161A3 (en) New labelling strategies for the sensitive detection of analytes
WO2001042502A3 (en) Methods for disease detection
WO2004094986A3 (en) System and method for electrochemical detection of biological compounds
WO2003043487A3 (en) Method of using a non-antibody protein to detect and measure an analyte
WO2001050129A3 (en) Compensation for non-specific signals in quantitative immunoassays
CA2331688A1 (en) Detection of a target in a sample
EP1156329A3 (de) Molekular-Modifikations-Assays
WO2004074310A8 (en) Mycobacterial diagnostics
WO2003036256A3 (en) Method and apparatus for increasing the dynamic range and accuracy of binding assays
WO2006026601A3 (en) Homogeneous enzyme immunoassay for oral fluid
AU6104598A (en) Method for isolating, in particular for detecting or quantifying an analyte in amedium
WO1999016781A3 (de) Automatisierbare universell anwendbare probenvorbereitungsmethode
ATE403153T1 (de) Test zum direkten nachweisen einer rs virus bezogenen biologischen zelle in einer körperflüssigkeitsprobe
WO2005003394A3 (en) Bio-barcode based detection of target analytes
WO2003023051A3 (en) Lateral flow test format for enzyme assays
CA2289043A1 (en) Method for detecting or predicting ischemic disorders
WO2006071970A3 (en) Methods for the detection of analytes in a sample
WO2003054225A3 (de) Verfahren zur bestimmung von nukleinsäureanalyten
WO2002035228A3 (de) Verfahren und testkit zum nachweis von analyten in einer probe
WO2002064829A3 (en) Biochemical method and apparatus for detecting genetic characteristics
WO2002065123A3 (en) Biochemical method and apparatus for detecting protein characteristics
WO2002064823A3 (de) Verfahren zum nachweis und/oder zur quantifizierung eines analyten
EP1249503A3 (de) Multiplex Gen Analyse auf einem mobilen festen Träger
WO1996019731A3 (en) Differential timing method for detecting multiple analytes in a test sample
WO2002076608A3 (de) Verfahren zur herstellung eines arrays zur detektion von komponenten aus einer biologischen probe

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
REF Corresponds to

Ref document number: 10290517

Country of ref document: DE

Date of ref document: 20031211

Kind code of ref document: P

WWE Wipo information: entry into national phase

Ref document number: 10290517

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 10467884

Country of ref document: US

122 Ep: pct application non-entry in european phase
WWE Wipo information: entry into national phase

Ref document number: 200405453

Country of ref document: ZA

REG Reference to national code

Ref country code: DE

Ref legal event code: 8607

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: JP