ES2114066T3 - Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico. - Google Patents
Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico.Info
- Publication number
- ES2114066T3 ES2114066T3 ES93919132T ES93919132T ES2114066T3 ES 2114066 T3 ES2114066 T3 ES 2114066T3 ES 93919132 T ES93919132 T ES 93919132T ES 93919132 T ES93919132 T ES 93919132T ES 2114066 T3 ES2114066 T3 ES 2114066T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- pct
- elimination
- test results
- analyte nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Saccharide Compounds (AREA)
Abstract
EL PRESENTE INVENTO SE REFIERE A UN METODO PARA LA ELIMINACION DE PRUEBAS FALSAS NEGATIVAS EN ENSAYOS PARA LA DETECCION DE ACIDO NUCLEICO ANALITO AMPLIFICADO EN UNA MUESTRA. ANTES DE LA AMPLIFICACION, SE AÑADE UN CONTROL INTERNO A LA MUESTRA QUE, COMPRENDE UN ACIDO NUCLEICO DISTINGUIBLE DEL ACIDO NUCLEICO ANALITO, QUE SE PUEDE AMPLIFICAR CON LOS MISMOS REACTIVOS DE AMPLIFICACION QUE EL ACIDO NUCLEICO ANALITO. PREFERIBLEMENTE EL CONTROL INTERNO COMPRENDE UNA SECUENCIA DE ACIDO NUCLEICO QUE CORRESPONDE AL ACIDO NUCLEICO ANALITO QUE HA SIDO CAMBIADO PARA DISTINGUIRLO DEL ACIDO NUCLEICO ANALITO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP92202563 | 1992-08-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2114066T3 true ES2114066T3 (es) | 1998-05-16 |
Family
ID=8210876
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93919132T Expired - Lifetime ES2114066T3 (es) | 1992-08-24 | 1993-08-20 | Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico. |
Country Status (14)
Country | Link |
---|---|
US (1) | US5770360A (es) |
EP (1) | EP0656955B1 (es) |
JP (1) | JP3560974B2 (es) |
KR (1) | KR100292925B1 (es) |
AT (1) | ATE162226T1 (es) |
AU (1) | AU685396B2 (es) |
CA (1) | CA2143202C (es) |
DE (1) | DE69316372T2 (es) |
DK (1) | DK0656955T3 (es) |
ES (1) | ES2114066T3 (es) |
FI (1) | FI111739B (es) |
GR (1) | GR3026631T3 (es) |
WO (1) | WO1994004706A1 (es) |
ZA (1) | ZA936015B (es) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AT401270B (de) * | 1994-09-26 | 1996-07-25 | Immuno Ag | Verfahren zur quantifizierung von genomischer dna |
ES2093554B1 (es) * | 1995-02-17 | 1997-07-01 | Inst De Salud Carlos Iii | Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de agentes infecciosos relacionados. |
EP0851941B1 (en) * | 1995-09-20 | 1999-11-17 | E.I. Du Pont De Nemours And Company | Control for pcr |
US7070962B1 (en) | 1996-02-01 | 2006-07-04 | Aventis Pharmaceuticals Inc. | Positive controls in polynucleotide amplification |
EP0819181B1 (en) * | 1996-02-01 | 2002-08-07 | Aventis Pharmaceuticals, Inc. | Positive controls for use in polynucleotide amplification |
BE1010608A3 (fr) * | 1996-09-09 | 1998-11-03 | Jose Remacle | Procede de quantification d'une sequence d'acides nucleiques. |
WO1998011253A2 (fr) * | 1996-09-09 | 1998-03-19 | Remacle Jose | Procede et trousse de diagnostic et/ou de quantification par hybridation de type sandwich de sequences d'acides nucleiques sur support solide |
US5888740A (en) * | 1997-09-19 | 1999-03-30 | Genaco Biomedical Products, Inc. | Detection of aneuploidy and gene deletion by PCR-based gene- dose co-amplification of chromosome specific sequences with synthetic sequences with synthetic internal controls |
US6365346B1 (en) * | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
AT407160B (de) * | 1998-06-04 | 2001-01-25 | Immuno Ag | Verfahren zur bestimmung von antigenen |
GB9902422D0 (en) * | 1999-02-03 | 1999-03-24 | Lgc Teddington Limited | Reference material for nucleic acid amplification |
AT409383B (de) * | 1999-12-22 | 2002-07-25 | Baxter Ag | Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe |
EP1236805B1 (en) * | 2001-03-02 | 2008-05-07 | Roche Diagnostics GmbH | A method for the determination of a nucleic acid using a control |
EP1236804A1 (en) * | 2001-03-02 | 2002-09-04 | Boehringer Mannheim Gmbh | A method for determination of a nucleic acid using a control |
US20050147984A1 (en) * | 2001-08-31 | 2005-07-07 | Clondiag Chip Technologies Gmbh | Interaction detection on several probe arrays |
US6686162B2 (en) * | 2001-12-04 | 2004-02-03 | Quest Diagnostics Investments, Incorporated | Oligonucleotides and methods for detecting Borrelia burgdorferi |
US20040023220A1 (en) * | 2002-07-23 | 2004-02-05 | Lawrence Greenfield | Integrated method for PCR cleanup and oligonucleotide removal |
US20040096829A1 (en) * | 2002-11-14 | 2004-05-20 | Allaire Normand E. | Absolute quantitation of nucleic acids by RT-PCR |
FR2921064A1 (fr) * | 2007-09-14 | 2009-03-20 | Biomerieux Sa | Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence du gene e1 du virus du chikungunya |
JP5822824B2 (ja) * | 2009-06-04 | 2015-11-24 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 複合核酸の増幅 |
CN102625851A (zh) * | 2009-07-07 | 2012-08-01 | 新加坡科技研究局 | 人免疫缺陷病毒1型(hiv)检测方法及其试剂盒 |
JP5999817B2 (ja) * | 2011-02-25 | 2016-10-05 | ノバルティス アーゲー | 外因性の内部陽性対照 |
EP3492604B1 (en) * | 2011-11-04 | 2021-01-06 | Gen-Probe Incorporated | Molecular assay reagents and methods |
EP2852690B1 (en) * | 2012-05-22 | 2020-08-26 | The Johns Hopkins University | A QUANTITATIVE MULTIPLEX METHYLATION SPECIFIC PCR METHOD- cMethDNA, REAGENTS, AND ITS USE |
CN104560982B (zh) * | 2015-01-28 | 2018-05-18 | 中国医科大学附属第一医院 | 不同种属微生物间种类和丰度比较的人工外源性参照分子 |
CN111479929A (zh) * | 2017-11-24 | 2020-07-31 | 株式会社理光 | 检测判断方法、检测判断装置、检测判断程序和装置 |
JP7322386B2 (ja) * | 2017-11-24 | 2023-08-08 | 株式会社リコー | 検出判定方法、検出判定装置、検出判定プログラム、及びデバイス |
WO2019103122A1 (en) * | 2017-11-24 | 2019-05-31 | Ricoh Company, Ltd. | Detection determining method, detection determining device, detection determining program, and device |
KR102575756B1 (ko) * | 2018-05-10 | 2023-09-07 | 주식회사 씨젠 | 내부 대조군으로 장내 정상세균총을 이용하여 시료로부터 장내 미생물을 검출하는 방법 |
US20230332217A1 (en) * | 2020-08-28 | 2023-10-19 | Seegene, Inc. | Method for positive control reaction using pre-positive control composition |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4626501A (en) * | 1983-09-02 | 1986-12-02 | Integrated Genetics, Inc. | Labeled DNA |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US5356774A (en) * | 1986-04-16 | 1994-10-18 | The Trustees Of Columbia University In The City Of New York | Replicative RNA-based amplification/detection systems |
EP0397753B1 (en) * | 1988-01-29 | 1996-06-12 | Advanced Riverina Holdings Limited | Determination of genetic sex in ruminants using y-chromosome-specific polynucleotides |
CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
ES2092527T3 (es) * | 1990-06-08 | 1996-12-01 | Behringwerke Ag | Metodo para la determinacion cuantitativa de secuencias de adn. |
GB9016163D0 (en) * | 1990-07-24 | 1990-09-05 | Cemu Bioteknik | Chemical method |
US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
ES2214472T3 (es) * | 1991-08-02 | 2004-09-16 | Biomerieux B.V. | Cuantificacion de acidos nucleicos. |
FR2683827B1 (fr) * | 1991-11-15 | 1994-03-04 | Institut Nal Sante Recherc Medic | Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique. |
US5457027A (en) * | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
-
1993
- 1993-08-17 ZA ZA936015A patent/ZA936015B/xx unknown
- 1993-08-20 DK DK93919132T patent/DK0656955T3/da active
- 1993-08-20 AU AU49513/93A patent/AU685396B2/en not_active Expired
- 1993-08-20 EP EP93919132A patent/EP0656955B1/en not_active Expired - Lifetime
- 1993-08-20 DE DE69316372T patent/DE69316372T2/de not_active Expired - Lifetime
- 1993-08-20 AT AT93919132T patent/ATE162226T1/de active
- 1993-08-20 WO PCT/EP1993/002248 patent/WO1994004706A1/en active IP Right Grant
- 1993-08-20 CA CA002143202A patent/CA2143202C/en not_active Expired - Lifetime
- 1993-08-20 US US08/392,932 patent/US5770360A/en not_active Expired - Lifetime
- 1993-08-20 KR KR1019950700692A patent/KR100292925B1/ko not_active IP Right Cessation
- 1993-08-20 ES ES93919132T patent/ES2114066T3/es not_active Expired - Lifetime
- 1993-08-20 JP JP50591594A patent/JP3560974B2/ja not_active Expired - Lifetime
-
1995
- 1995-02-23 FI FI950836A patent/FI111739B/fi not_active IP Right Cessation
-
1998
- 1998-04-14 GR GR980400827T patent/GR3026631T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
ATE162226T1 (de) | 1998-01-15 |
ZA936015B (en) | 1994-03-10 |
WO1994004706A1 (en) | 1994-03-03 |
EP0656955B1 (en) | 1998-01-14 |
AU4951393A (en) | 1994-03-15 |
JP3560974B2 (ja) | 2004-09-02 |
JPH08500732A (ja) | 1996-01-30 |
CA2143202A1 (en) | 1994-03-03 |
FI111739B (fi) | 2003-09-15 |
EP0656955A1 (en) | 1995-06-14 |
DE69316372D1 (de) | 1998-02-19 |
DK0656955T3 (da) | 1998-09-14 |
KR950703065A (ko) | 1995-08-23 |
KR100292925B1 (ko) | 2006-03-31 |
FI950836A0 (fi) | 1995-02-23 |
DE69316372T2 (de) | 1998-08-27 |
US5770360A (en) | 1998-06-23 |
AU685396B2 (en) | 1998-01-22 |
GR3026631T3 (en) | 1998-07-31 |
CA2143202C (en) | 2007-06-12 |
FI950836A (fi) | 1995-02-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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