ES2114066T3 - Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico. - Google Patents

Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico.

Info

Publication number
ES2114066T3
ES2114066T3 ES93919132T ES93919132T ES2114066T3 ES 2114066 T3 ES2114066 T3 ES 2114066T3 ES 93919132 T ES93919132 T ES 93919132T ES 93919132 T ES93919132 T ES 93919132T ES 2114066 T3 ES2114066 T3 ES 2114066T3
Authority
ES
Spain
Prior art keywords
nucleic acid
pct
elimination
test results
analyte nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93919132T
Other languages
English (en)
Inventor
Tim Kievits
Peter Franklin Lens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo Nobel NV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Application granted granted Critical
Publication of ES2114066T3 publication Critical patent/ES2114066T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)

Abstract

EL PRESENTE INVENTO SE REFIERE A UN METODO PARA LA ELIMINACION DE PRUEBAS FALSAS NEGATIVAS EN ENSAYOS PARA LA DETECCION DE ACIDO NUCLEICO ANALITO AMPLIFICADO EN UNA MUESTRA. ANTES DE LA AMPLIFICACION, SE AÑADE UN CONTROL INTERNO A LA MUESTRA QUE, COMPRENDE UN ACIDO NUCLEICO DISTINGUIBLE DEL ACIDO NUCLEICO ANALITO, QUE SE PUEDE AMPLIFICAR CON LOS MISMOS REACTIVOS DE AMPLIFICACION QUE EL ACIDO NUCLEICO ANALITO. PREFERIBLEMENTE EL CONTROL INTERNO COMPRENDE UNA SECUENCIA DE ACIDO NUCLEICO QUE CORRESPONDE AL ACIDO NUCLEICO ANALITO QUE HA SIDO CAMBIADO PARA DISTINGUIRLO DEL ACIDO NUCLEICO ANALITO.
ES93919132T 1992-08-24 1993-08-20 Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico. Expired - Lifetime ES2114066T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP92202563 1992-08-24

Publications (1)

Publication Number Publication Date
ES2114066T3 true ES2114066T3 (es) 1998-05-16

Family

ID=8210876

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93919132T Expired - Lifetime ES2114066T3 (es) 1992-08-24 1993-08-20 Eliminacion de resultados de ensayo falso negativos en la deteccion de acido nucleico.

Country Status (14)

Country Link
US (1) US5770360A (es)
EP (1) EP0656955B1 (es)
JP (1) JP3560974B2 (es)
KR (1) KR100292925B1 (es)
AT (1) ATE162226T1 (es)
AU (1) AU685396B2 (es)
CA (1) CA2143202C (es)
DE (1) DE69316372T2 (es)
DK (1) DK0656955T3 (es)
ES (1) ES2114066T3 (es)
FI (1) FI111739B (es)
GR (1) GR3026631T3 (es)
WO (1) WO1994004706A1 (es)
ZA (1) ZA936015B (es)

Families Citing this family (30)

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Publication number Priority date Publication date Assignee Title
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
ES2093554B1 (es) * 1995-02-17 1997-07-01 Inst De Salud Carlos Iii Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de agentes infecciosos relacionados.
EP0851941B1 (en) * 1995-09-20 1999-11-17 E.I. Du Pont De Nemours And Company Control for pcr
US7070962B1 (en) 1996-02-01 2006-07-04 Aventis Pharmaceuticals Inc. Positive controls in polynucleotide amplification
EP0819181B1 (en) * 1996-02-01 2002-08-07 Aventis Pharmaceuticals, Inc. Positive controls for use in polynucleotide amplification
BE1010608A3 (fr) * 1996-09-09 1998-11-03 Jose Remacle Procede de quantification d'une sequence d'acides nucleiques.
WO1998011253A2 (fr) * 1996-09-09 1998-03-19 Remacle Jose Procede et trousse de diagnostic et/ou de quantification par hybridation de type sandwich de sequences d'acides nucleiques sur support solide
US5888740A (en) * 1997-09-19 1999-03-30 Genaco Biomedical Products, Inc. Detection of aneuploidy and gene deletion by PCR-based gene- dose co-amplification of chromosome specific sequences with synthetic sequences with synthetic internal controls
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
AT407160B (de) * 1998-06-04 2001-01-25 Immuno Ag Verfahren zur bestimmung von antigenen
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
AT409383B (de) * 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
EP1236805B1 (en) * 2001-03-02 2008-05-07 Roche Diagnostics GmbH A method for the determination of a nucleic acid using a control
EP1236804A1 (en) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh A method for determination of a nucleic acid using a control
US20050147984A1 (en) * 2001-08-31 2005-07-07 Clondiag Chip Technologies Gmbh Interaction detection on several probe arrays
US6686162B2 (en) * 2001-12-04 2004-02-03 Quest Diagnostics Investments, Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
US20040023220A1 (en) * 2002-07-23 2004-02-05 Lawrence Greenfield Integrated method for PCR cleanup and oligonucleotide removal
US20040096829A1 (en) * 2002-11-14 2004-05-20 Allaire Normand E. Absolute quantitation of nucleic acids by RT-PCR
FR2921064A1 (fr) * 2007-09-14 2009-03-20 Biomerieux Sa Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence du gene e1 du virus du chikungunya
JP5822824B2 (ja) * 2009-06-04 2015-11-24 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 複合核酸の増幅
CN102625851A (zh) * 2009-07-07 2012-08-01 新加坡科技研究局 人免疫缺陷病毒1型(hiv)检测方法及其试剂盒
JP5999817B2 (ja) * 2011-02-25 2016-10-05 ノバルティス アーゲー 外因性の内部陽性対照
EP3492604B1 (en) * 2011-11-04 2021-01-06 Gen-Probe Incorporated Molecular assay reagents and methods
EP2852690B1 (en) * 2012-05-22 2020-08-26 The Johns Hopkins University A QUANTITATIVE MULTIPLEX METHYLATION SPECIFIC PCR METHOD- cMethDNA, REAGENTS, AND ITS USE
CN104560982B (zh) * 2015-01-28 2018-05-18 中国医科大学附属第一医院 不同种属微生物间种类和丰度比较的人工外源性参照分子
CN111479929A (zh) * 2017-11-24 2020-07-31 株式会社理光 检测判断方法、检测判断装置、检测判断程序和装置
JP7322386B2 (ja) * 2017-11-24 2023-08-08 株式会社リコー 検出判定方法、検出判定装置、検出判定プログラム、及びデバイス
WO2019103122A1 (en) * 2017-11-24 2019-05-31 Ricoh Company, Ltd. Detection determining method, detection determining device, detection determining program, and device
KR102575756B1 (ko) * 2018-05-10 2023-09-07 주식회사 씨젠 내부 대조군으로 장내 정상세균총을 이용하여 시료로부터 장내 미생물을 검출하는 방법
US20230332217A1 (en) * 2020-08-28 2023-10-19 Seegene, Inc. Method for positive control reaction using pre-positive control composition

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4626501A (en) * 1983-09-02 1986-12-02 Integrated Genetics, Inc. Labeled DNA
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5356774A (en) * 1986-04-16 1994-10-18 The Trustees Of Columbia University In The City Of New York Replicative RNA-based amplification/detection systems
EP0397753B1 (en) * 1988-01-29 1996-06-12 Advanced Riverina Holdings Limited Determination of genetic sex in ruminants using y-chromosome-specific polynucleotides
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
ES2092527T3 (es) * 1990-06-08 1996-12-01 Behringwerke Ag Metodo para la determinacion cuantitativa de secuencias de adn.
GB9016163D0 (en) * 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ES2214472T3 (es) * 1991-08-02 2004-09-16 Biomerieux B.V. Cuantificacion de acidos nucleicos.
FR2683827B1 (fr) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions

Also Published As

Publication number Publication date
ATE162226T1 (de) 1998-01-15
ZA936015B (en) 1994-03-10
WO1994004706A1 (en) 1994-03-03
EP0656955B1 (en) 1998-01-14
AU4951393A (en) 1994-03-15
JP3560974B2 (ja) 2004-09-02
JPH08500732A (ja) 1996-01-30
CA2143202A1 (en) 1994-03-03
FI111739B (fi) 2003-09-15
EP0656955A1 (en) 1995-06-14
DE69316372D1 (de) 1998-02-19
DK0656955T3 (da) 1998-09-14
KR950703065A (ko) 1995-08-23
KR100292925B1 (ko) 2006-03-31
FI950836A0 (fi) 1995-02-23
DE69316372T2 (de) 1998-08-27
US5770360A (en) 1998-06-23
AU685396B2 (en) 1998-01-22
GR3026631T3 (en) 1998-07-31
CA2143202C (en) 2007-06-12
FI950836A (fi) 1995-02-23

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