KR910016770A - 글리코사미노글리칸-변형된 단백질 - Google Patents

글리코사미노글리칸-변형된 단백질 Download PDF

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KR910016770A
KR910016770A KR1019900016160A KR900016160A KR910016770A KR 910016770 A KR910016770 A KR 910016770A KR 1019900016160 A KR1019900016160 A KR 1019900016160A KR 900016160 A KR900016160 A KR 900016160A KR 910016770 A KR910016770 A KR 910016770A
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glycosaminoglycan
protein
modified protein
residue
excluding
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KR1019900016160A
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KR100188382B1 (ko
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가츄키요 사쿠라이
키요슈제 미야자키
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야마디니 와다루
세이카가쿠고교 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

글리코사미노글리칸-변형된 단백질
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 내지 3도는 실시예에서 수득된 글리코 사미노글리칸-변형된 단백질 및 글리코사미노글리칸과 단백질의 혼합물의 전기영동 패턴을 나타낸다.

Claims (9)

  1. 글리코사미노글리칸의 환원성 말단 당 잔기를 환원시키고 부분적으로 산화시켜 형성된 알데하이드 그룹에 단백질의 아미노 그룹이 결합된 글리코사미노글리칸-변형된 단백질.
  2. 일반식(Ⅰ)의 글리코사미노글리칸-변형된 단백질.
    상기식에서, P는 단백질로부터 n개의 아미노 그룹을 제외한 단백질 잔기이고, n은 1 내지 100의 정수이며, GAG는 글리코사미노글리칸의 환원성 말단 당 잔기를 제외한 글리코사미노글리칸이다.
  3. 일반식(Ⅱ)의 글리코사미노글리칸-변형된 단백질.
    상기식에서, P는 단백질로부터 n개의 아미노 그룹을 제외한 단백질 잔기이고, n은 1 내지 100의 정수이며, GAG는 글리코사미노글리칸으로부터 환원성 말단 당 잔기를 제외한 글리코사미노글리칸이다.
  4. 일반식(Ⅲ)의 글리코사미노글리칸-변형된 단백질.
    상기식에서, P는 단백질로부터 n개의 아미노 그룹을 제외한 단백질 잔기이고, n은 1 내지 100의 정수이며, GAG는 글리코사미노글리칸으로부터 환원성 말단 당 잔기를 제외한 글리코사미노글리칸이다.
  5. 글리코사미노글리칸의 우론산 잔기에 최소한 몇몇 카복실 그룹이 아미드 결합을 통하여 단백질에 결합된 글리코사미노글리칸-변형된 단백질.
  6. 제1항에 있어서, 글리코사미노글리칸 함량이, 단백질의 중량을 기준으로 하여, 1 내지 99.9중량%의 범위인 글리코사미노글리칸-변형된 단백질.
  7. 제1항에 있어서, 글리코사미노글리칸이 콜로민산, 히알우론산, 콘드로이틴, 콘드로이틴 설페이트, 테이추론산, 더마탄 설페이트, 헤파린, 헤파린 설페이트, 케라토설페이트, 케라토폴리설페이트 및 이의 유도체들 중에서 선택되는 글리코사미노글리칸-변형된 단백질.
  8. 글리코사미노글리칸을 환원성 말단 잔기-제한적 산화 방법, 카복실 그룹-활성화 법, 환원성 말단 잔기-락톤화 방법 또는 시아노겐 브로마이드 활성화 방법에 의해 활성화시킨 후, 활성화된 글리코사미노글리칸을 단백질과 반응시킴으로 특징으로 하는 제1항에 따른 글리코사미노글리칸-변형된 단백질의 제조방법.
  9. 제1항에 따른 글리코사미노글리칸-변형된 단백질 및 약제학적으로 허용되는 담체 또는 희석제를 함유하는 약제학적 조성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900016160A 1990-03-30 1990-10-12 글리코사미노글리칸 변형된 단백질 및 이를 포함하는 약제학적 조성물 _ KR100188382B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2081163A JP2975632B2 (ja) 1990-03-30 1990-03-30 グリコサミノグリカン修飾プロテイン
JP2-81163 1990-03-30

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KR910016770A true KR910016770A (ko) 1991-11-05
KR100188382B1 KR100188382B1 (ko) 1999-06-01

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US (1) US5310881A (ko)
EP (1) EP0454898B1 (ko)
JP (1) JP2975632B2 (ko)
KR (1) KR100188382B1 (ko)
AT (1) ATE135375T1 (ko)
AU (1) AU649416B2 (ko)
CA (1) CA2027447A1 (ko)
DE (1) DE69025920T2 (ko)
DK (1) DK0454898T3 (ko)
ES (1) ES2083989T3 (ko)
FI (1) FI102836B (ko)
NO (1) NO179044C (ko)

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Also Published As

Publication number Publication date
ES2083989T3 (es) 1996-05-01
NO904378L (no) 1991-10-01
EP0454898A1 (en) 1991-11-06
AU6450690A (en) 1991-10-31
JPH03284698A (ja) 1991-12-16
US5310881A (en) 1994-05-10
DE69025920D1 (de) 1996-04-18
NO179044C (no) 1996-07-24
DK0454898T3 (da) 1996-04-15
EP0454898B1 (en) 1996-03-13
FI102836B1 (fi) 1999-02-26
JP2975632B2 (ja) 1999-11-10
FI905003A0 (fi) 1990-10-11
CA2027447A1 (en) 1991-10-01
ATE135375T1 (de) 1996-03-15
NO904378D0 (no) 1990-10-10
KR100188382B1 (ko) 1999-06-01
FI905003A (fi) 1991-10-01
FI102836B (fi) 1999-02-26
AU649416B2 (en) 1994-05-26
NO179044B (no) 1996-04-15
DE69025920T2 (de) 1996-08-14

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