KR860008995A - 신규한 리파제 기질 및 리파제의 광학적 정량을 위한 방법 및 조성물 - Google Patents

신규한 리파제 기질 및 리파제의 광학적 정량을 위한 방법 및 조성물 Download PDF

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KR860008995A
KR860008995A KR1019860003481A KR860003481A KR860008995A KR 860008995 A KR860008995 A KR 860008995A KR 1019860003481 A KR1019860003481 A KR 1019860003481A KR 860003481 A KR860003481 A KR 860003481A KR 860008995 A KR860008995 A KR 860008995A
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lipase
lipase substrate
glycero
rac
ester
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KR1019860003481A
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KR890003946B1 (ko
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뉴만 울리히
뉴만 율리히
주니우스 마티나
바쯔 한스-조르그
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뵈링거 만하임 게엠배하
독트르 다움 독트르. 포우쿠에트
뵈링거 만하임 게엠베하
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D265/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D265/28—1,4-Oxazines; Hydrogenated 1,4-oxazines
    • C07D265/34—1,4-Oxazines; Hydrogenated 1,4-oxazines condensed with carbocyclic rings
    • C07D265/38—[b, e]-condensed with two six-membered rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases

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  • Life Sciences & Earth Sciences (AREA)
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  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Indole Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

신규한 리파제 기질 및 리파제의 광학적 정량을 위한 방법 및 조성물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (12)

  1. 다음 일반식의 리파제 기질이
    [식중, A는 C16까지의 알킬렌 또는 알케닐렌기, R 및 R1은 같거나 다를 수 있고, 각각 C20까지의 알킬, 알케닐 또는 아실기 또는 알킬 부분이 C8까지의 인의로 알킬-치환된 아릴 또는 아랄킬기, R 및 R1의 하나는 수소 원자, X는 방향족 히드록시 또는 티올 화합물의 잔사, Y 및 Z는 독립적으로 -S- 또는 -O-, Z는 또한 -CH2-] 리파제를 포함하는 샘플의 작용을 받고 유리된 방향족 히드록시 또는 티올 화합물이 임의로 직접적으로 정량되거나 또는, 적당한 색원체와 결합한 후에, 형성된 색이 광학적으로 정량되는 것으로 구성되는 리파제를 광학적으로 정량하는 방법.
  2. 제1항에 있어서, 리파제 기질의 R 및/또는 R1가 C8-C12를 포함하는 방법.
  3. 제1항 또는 제2항에 있어서, 리파제 기질의 A가 C3-C7를 포함하는 방법.
  4. 전기한 항의 어느 한 항에 있어서, 리파제 기질의 X가 치환되지 않았거나 또는 치환된 레소루핀, 큭로로페놀 레드, 인독실, 나프톨, 티오페놀, 티오플루오로세인 또는 페놀기인 방법.
  5. 전기한 항의 어느 한 항에 있어서, 리파제 기질이 1,2-0-디옥틸-rac-글리세로-3-아겔라인산 레소루핀 에스테르인 방법.
  6. 전기한 항의 어느 한 항에 있어서, 리파제 기질이 1,2-0-디데실-rac-글리세로-3-피멜린산 레소루핀 에스테르인 방법.
  7. 전기한 항의 어느 한 항에 있어서, 리파제 기질이 1,2-0-디데실-rac-글리세로-3-글루타르산 레소루핀 에스테르인 방법.
  8. 전기한 항의 어느 한 항에 있어서, 리파제 기질이 1,2-0-디도데실-rac-글리세로-3-글루타르산 레소루핀 에스테르인 방법.
  9. 제1항에 있어서, 0.1-10mg/ml 의 염 온도에서 정량하는 방법.
  10. 담즙산 알카리 금속 염, 콜리파제, 염, 우레아 및/또는 색원체 결합제 뿐만 아니라, 제1항에서 제8항까지 중의 최소한 한 화합물 및 완충 물질을 포함하는, 리파제의 광학적 정량용의 조성물.
  11. 제10항에 있어서, 각각이 테스트시 용액으로 준비된, 0.05-10mg/ml 기질, 2-50mg/ml데스옥시콜레이트, 0.001-0.01mg/ml콜리파제, 1-100mg/ml우레아,0.1-10mg/ml염화 나트륨 및 1-50mg/ml 완충 물질을 포함하는 조성물.
  12. 제10항 또는 제11항에 있어서, 담체 재료내에 함유된 상태로 존재하는 조성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860003481A 1985-05-03 1986-05-03 리파제 정량방법 KR890003946B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DEP-35-16-001.2 1985-05-03
DE3516001.2 1985-05-03
DE19853516001 DE3516001A1 (de) 1985-05-03 1985-05-03 Lipasefarbtest

Publications (2)

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KR860008995A true KR860008995A (ko) 1986-12-19
KR890003946B1 KR890003946B1 (ko) 1989-10-13

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US (2) US4847376A (ko)
EP (1) EP0207252B1 (ko)
JP (1) JPH0687800B2 (ko)
KR (1) KR890003946B1 (ko)
AT (1) ATE78874T1 (ko)
AU (1) AU564688B2 (ko)
CA (1) CA1285941C (ko)
DE (2) DE3516001A1 (ko)
ES (1) ES8704470A1 (ko)
SU (1) SU1604167A3 (ko)
ZA (1) ZA863303B (ko)

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US5308766A (en) * 1989-08-29 1994-05-03 The Regents Of The University Of California Hydrolytic enzyme inhibitors/inactivators and methods for using same
DE69029269T2 (de) * 1989-08-29 1997-05-28 Univ California Neue inhibitoren für hydrolytische enzyme und substrate und bestimmungsverfahren, selbige einschliessende verfahren und testsätze
US5352673A (en) * 1989-08-29 1994-10-04 Dennis Edward A Prodrugs
DE4029845A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Verfahren zur bestimmung von phospholipase in koerperfluessigkeiten
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JP4018237B2 (ja) * 1998-05-11 2007-12-05 株式会社タウンズ 酵素活性測定用リパーゼ基質溶液およびリパーゼ活性測定用試薬キット
US20070122862A1 (en) * 2003-10-29 2007-05-31 Novozymes A/S Screening for lipolytic enzymes or amidase activity
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CA2786255A1 (en) * 2009-12-31 2011-07-07 Organomed Corporation Formulations from natural products, turmeric, and aspirin
CN103983626B (zh) * 2014-05-21 2017-08-29 北京安生绿源科技有限公司 一种脂肪酶检测试剂的脂肪酶快速检测方法
WO2016024549A1 (ja) * 2014-08-12 2016-02-18 株式会社シノテスト リパーゼ活性測定用基質溶液の製造方法及び製造の簡略化方法
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ES2783857T3 (es) 2015-06-19 2020-09-18 Shino Test Corp Solución de sustrato para medir la actividad de lipasa y método y reactivo para medir la actividad lipasa en una muestra
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EP0207252A3 (en) 1990-04-25
DE3516001A1 (de) 1986-11-06
ES8704470A1 (es) 1987-04-16
DE3686212D1 (en) 1992-09-03
ES554600A0 (es) 1987-04-16
AU564688B2 (en) 1987-08-20
ATE78874T1 (de) 1992-08-15
US4847376A (en) 1989-07-11
JPH0687800B2 (ja) 1994-11-09
AU5700186A (en) 1986-11-06
SU1604167A3 (ru) 1990-10-30
EP0207252A2 (de) 1987-01-07
US4988497A (en) 1991-01-29
EP0207252B1 (de) 1992-07-29
KR890003946B1 (ko) 1989-10-13
CA1285941C (en) 1991-07-09
ZA863303B (en) 1987-01-28
JPS61254197A (ja) 1986-11-11

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