GB1501561A - Method for measuring the activity of lecithin cholesterol acyl transferase and lecithin substrates solution useful therefor - Google Patents

Method for measuring the activity of lecithin cholesterol acyl transferase and lecithin substrates solution useful therefor

Info

Publication number
GB1501561A
GB1501561A GB1773376A GB1773376A GB1501561A GB 1501561 A GB1501561 A GB 1501561A GB 1773376 A GB1773376 A GB 1773376A GB 1773376 A GB1773376 A GB 1773376A GB 1501561 A GB1501561 A GB 1501561A
Authority
GB
United Kingdom
Prior art keywords
lecithin
acyl transferase
polyoxyethylene
activity
cholesterol acyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1773376A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Azwell Inc
Original Assignee
Nippon Shoji Kaisha Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP50053655A external-priority patent/JPS51129293A/en
Priority claimed from JP641676A external-priority patent/JPS5289994A/en
Application filed by Nippon Shoji Kaisha Ltd filed Critical Nippon Shoji Kaisha Ltd
Publication of GB1501561A publication Critical patent/GB1501561A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1501561 Determination of lecithin cholesterol acyl transferase NIPPON SHOJI KAISHA Ltd 30 April 1976 [2 May 1975 22 Jan 1976] 17733/76 Heading G1B A lecithin substrate solution for the measurement of the activity of lecithin cholesterol acyl transferase comprises 2À5 to 10 mg/ml lecithin and at least 0À01% (w/v) of a non-ionic surfactant in a buffer solution. Lecithin substrates exemplified are:- dipalmitoyl-, dimyristoyl- and distearyl lecithin. The non-ionic surfactant may be a polyoxyethylene alkyl ether, polyoxyethylene alkyl phenol ether, a polyoxyethylene sorbitan alkyl ester, a polyoxyethylene alkyl ester, a polyoxyethylene alkylamine, a polyoxyethylene alkyl amide or a sorbitan alkyl ester. The free cholesterol formed on incubating a test sample containing the transferase with the substrate of the invention may be determined using a reagent comprisng cholesterol oxidase, peroxidase, phenol and 4-amino-antipyrine.
GB1773376A 1975-05-02 1976-04-30 Method for measuring the activity of lecithin cholesterol acyl transferase and lecithin substrates solution useful therefor Expired GB1501561A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP50053655A JPS51129293A (en) 1975-05-02 1975-05-02 Measurement method of activity of lecithin cholesteral acyl transferas e
JP641676A JPS5289994A (en) 1976-01-22 1976-01-22 Liquidous lecithin substrate for measurements of activity of lecithin cholesterol acyl trnsferase

Publications (1)

Publication Number Publication Date
GB1501561A true GB1501561A (en) 1978-02-15

Family

ID=26340551

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1773376A Expired GB1501561A (en) 1975-05-02 1976-04-30 Method for measuring the activity of lecithin cholesterol acyl transferase and lecithin substrates solution useful therefor

Country Status (2)

Country Link
DE (1) DE2619252C3 (en)
GB (1) GB1501561A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4499184A (en) * 1982-06-01 1985-02-12 Eastman Kodak Company Analysis for total cholesterol using lecithin:cholesterol acyl transferase (LCAT)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4499184A (en) * 1982-06-01 1985-02-12 Eastman Kodak Company Analysis for total cholesterol using lecithin:cholesterol acyl transferase (LCAT)

Also Published As

Publication number Publication date
DE2619252A1 (en) 1976-11-04
DE2619252B2 (en) 1977-08-11
DE2619252C3 (en) 1979-06-13

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee