JPS56131398A - Quantitative determination of hydrogen peroxide - Google Patents

Quantitative determination of hydrogen peroxide

Info

Publication number
JPS56131398A
JPS56131398A JP3343480A JP3343480A JPS56131398A JP S56131398 A JPS56131398 A JP S56131398A JP 3343480 A JP3343480 A JP 3343480A JP 3343480 A JP3343480 A JP 3343480A JP S56131398 A JPS56131398 A JP S56131398A
Authority
JP
Japan
Prior art keywords
hydrogen peroxide
aniline derivative
aminoantipyrine
peroxidase
alkyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3343480A
Other languages
Japanese (ja)
Inventor
Shozo Abe
Yuzo Kosaka
Yasuyuki Matsumoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Priority to JP3343480A priority Critical patent/JPS56131398A/en
Publication of JPS56131398A publication Critical patent/JPS56131398A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine quantitatively hydrogen peroxide by colorimetry using a luminescence system having high sensitivity and improved coloring stability, by reacting hydrogen peroxide with 4-aminoantipyrine and an aniline derivative in the presence of peroxidase.
CONSTITUTION: Hydrogen peroxide is reacted with 4-aminoantipyrine and an aniline derivative shown by the formula [R1 and R2 are CnH2nNHSO2.R (n is 2W4; R is hydroxyl, straight-chain or branched chain 1W5C alkyl, or phenyl;), R3 and R4 are H, alkyl, or alkoxyalkyl] in the presence of peroxidase. N-Ethyl-N-(β-methylsulfonamidoethyl)-m-toluidine, etc. may be cited as the N-substituted aniline derivative used. By the use of the above-mentioned method, hydrogen peroxide, which is produced by reacting not only glycose or cholesterol in serum but also uric acid relatively in low concentration in humors with uricase, can be introduced to luminescence system in high sensitiveness, to measure uric acid in serum easily.
COPYRIGHT: (C)1981,JPO&Japio
JP3343480A 1980-03-18 1980-03-18 Quantitative determination of hydrogen peroxide Pending JPS56131398A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3343480A JPS56131398A (en) 1980-03-18 1980-03-18 Quantitative determination of hydrogen peroxide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3343480A JPS56131398A (en) 1980-03-18 1980-03-18 Quantitative determination of hydrogen peroxide

Publications (1)

Publication Number Publication Date
JPS56131398A true JPS56131398A (en) 1981-10-14

Family

ID=12386423

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3343480A Pending JPS56131398A (en) 1980-03-18 1980-03-18 Quantitative determination of hydrogen peroxide

Country Status (1)

Country Link
JP (1) JPS56131398A (en)

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