JPS56131398A - Quantitative determination of hydrogen peroxide - Google Patents
Quantitative determination of hydrogen peroxideInfo
- Publication number
- JPS56131398A JPS56131398A JP3343480A JP3343480A JPS56131398A JP S56131398 A JPS56131398 A JP S56131398A JP 3343480 A JP3343480 A JP 3343480A JP 3343480 A JP3343480 A JP 3343480A JP S56131398 A JPS56131398 A JP S56131398A
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen peroxide
- aniline derivative
- aminoantipyrine
- peroxidase
- alkyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To determine quantitatively hydrogen peroxide by colorimetry using a luminescence system having high sensitivity and improved coloring stability, by reacting hydrogen peroxide with 4-aminoantipyrine and an aniline derivative in the presence of peroxidase.
CONSTITUTION: Hydrogen peroxide is reacted with 4-aminoantipyrine and an aniline derivative shown by the formula [R1 and R2 are CnH2nNHSO2.R (n is 2W4; R is hydroxyl, straight-chain or branched chain 1W5C alkyl, or phenyl;), R3 and R4 are H, alkyl, or alkoxyalkyl] in the presence of peroxidase. N-Ethyl-N-(β-methylsulfonamidoethyl)-m-toluidine, etc. may be cited as the N-substituted aniline derivative used. By the use of the above-mentioned method, hydrogen peroxide, which is produced by reacting not only glycose or cholesterol in serum but also uric acid relatively in low concentration in humors with uricase, can be introduced to luminescence system in high sensitiveness, to measure uric acid in serum easily.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3343480A JPS56131398A (en) | 1980-03-18 | 1980-03-18 | Quantitative determination of hydrogen peroxide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3343480A JPS56131398A (en) | 1980-03-18 | 1980-03-18 | Quantitative determination of hydrogen peroxide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS56131398A true JPS56131398A (en) | 1981-10-14 |
Family
ID=12386423
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3343480A Pending JPS56131398A (en) | 1980-03-18 | 1980-03-18 | Quantitative determination of hydrogen peroxide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56131398A (en) |
-
1980
- 1980-03-18 JP JP3343480A patent/JPS56131398A/en active Pending
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