JPS5661999A - Colorant for quantitative determination of hydrogen peroxide - Google Patents

Colorant for quantitative determination of hydrogen peroxide

Info

Publication number
JPS5661999A
JPS5661999A JP13673779A JP13673779A JPS5661999A JP S5661999 A JPS5661999 A JP S5661999A JP 13673779 A JP13673779 A JP 13673779A JP 13673779 A JP13673779 A JP 13673779A JP S5661999 A JPS5661999 A JP S5661999A
Authority
JP
Japan
Prior art keywords
hydrogen peroxide
colorant
quantitative determination
peroxidase
active ingredients
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP13673779A
Other languages
Japanese (ja)
Inventor
Tsutomu Okada
Tadashi Hishida
Minoru Muramatsu
Itaru Okada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP13673779A priority Critical patent/JPS5661999A/en
Publication of JPS5661999A publication Critical patent/JPS5661999A/en
Pending legal-status Critical Current

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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To obtain a colorant for quantitative determination having improved coloring stability in neutral or weakly alkali, by blending peroxidase with 4-amino- antipyrine and an aniline derivative shown by a specific formula as active ingredients.
CONSTITUTION: (A) Peroxidase is blended with (B) 4-aminoantipyrine and (C) an aniline derivative shown by the formula I [R1 and R2 are 1W4C alkyl group; X is H or methoxy; one of Y or Z is H, and the other is NHCOR3, NHSO2R3, or NHCO2R3 (R3 is 1W4C alkyl)] as active ingredients to prepare a colorant for quantitative determination of hydrogen peroxide. The following conditions can be used in the reaction with hydrogen peroxide; a concentration of hydrogen peroxide is 0.0001W10mM, pH is 3W10.5, reaction temperature is 10W60°C, and reaction time is 1minW4hr.
COPYRIGHT: (C)1981,JPO&Japio
JP13673779A 1979-10-23 1979-10-23 Colorant for quantitative determination of hydrogen peroxide Pending JPS5661999A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13673779A JPS5661999A (en) 1979-10-23 1979-10-23 Colorant for quantitative determination of hydrogen peroxide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13673779A JPS5661999A (en) 1979-10-23 1979-10-23 Colorant for quantitative determination of hydrogen peroxide

Publications (1)

Publication Number Publication Date
JPS5661999A true JPS5661999A (en) 1981-05-27

Family

ID=15182320

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13673779A Pending JPS5661999A (en) 1979-10-23 1979-10-23 Colorant for quantitative determination of hydrogen peroxide

Country Status (1)

Country Link
JP (1) JPS5661999A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4396714A (en) * 1980-01-09 1983-08-02 Dojindo Laboratories N-Sulfoalkylaniline derivatives
US4556640A (en) * 1983-06-29 1985-12-03 Miles Laboratories, Inc. Stabilized test composition, device and method for the determination of peroxidatively active substances
US4672029A (en) * 1984-12-06 1987-06-09 Eastman Kodak Company Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4396714A (en) * 1980-01-09 1983-08-02 Dojindo Laboratories N-Sulfoalkylaniline derivatives
US4556640A (en) * 1983-06-29 1985-12-03 Miles Laboratories, Inc. Stabilized test composition, device and method for the determination of peroxidatively active substances
US4672029A (en) * 1984-12-06 1987-06-09 Eastman Kodak Company Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes

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