JPS5661999A - Colorant for quantitative determination of hydrogen peroxide - Google Patents
Colorant for quantitative determination of hydrogen peroxideInfo
- Publication number
- JPS5661999A JPS5661999A JP13673779A JP13673779A JPS5661999A JP S5661999 A JPS5661999 A JP S5661999A JP 13673779 A JP13673779 A JP 13673779A JP 13673779 A JP13673779 A JP 13673779A JP S5661999 A JPS5661999 A JP S5661999A
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen peroxide
- colorant
- quantitative determination
- peroxidase
- active ingredients
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To obtain a colorant for quantitative determination having improved coloring stability in neutral or weakly alkali, by blending peroxidase with 4-amino- antipyrine and an aniline derivative shown by a specific formula as active ingredients.
CONSTITUTION: (A) Peroxidase is blended with (B) 4-aminoantipyrine and (C) an aniline derivative shown by the formula I [R1 and R2 are 1W4C alkyl group; X is H or methoxy; one of Y or Z is H, and the other is NHCOR3, NHSO2R3, or NHCO2R3 (R3 is 1W4C alkyl)] as active ingredients to prepare a colorant for quantitative determination of hydrogen peroxide. The following conditions can be used in the reaction with hydrogen peroxide; a concentration of hydrogen peroxide is 0.0001W10mM, pH is 3W10.5, reaction temperature is 10W60°C, and reaction time is 1minW4hr.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13673779A JPS5661999A (en) | 1979-10-23 | 1979-10-23 | Colorant for quantitative determination of hydrogen peroxide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13673779A JPS5661999A (en) | 1979-10-23 | 1979-10-23 | Colorant for quantitative determination of hydrogen peroxide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5661999A true JPS5661999A (en) | 1981-05-27 |
Family
ID=15182320
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP13673779A Pending JPS5661999A (en) | 1979-10-23 | 1979-10-23 | Colorant for quantitative determination of hydrogen peroxide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5661999A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4396714A (en) * | 1980-01-09 | 1983-08-02 | Dojindo Laboratories | N-Sulfoalkylaniline derivatives |
US4556640A (en) * | 1983-06-29 | 1985-12-03 | Miles Laboratories, Inc. | Stabilized test composition, device and method for the determination of peroxidatively active substances |
US4672029A (en) * | 1984-12-06 | 1987-06-09 | Eastman Kodak Company | Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes |
-
1979
- 1979-10-23 JP JP13673779A patent/JPS5661999A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4396714A (en) * | 1980-01-09 | 1983-08-02 | Dojindo Laboratories | N-Sulfoalkylaniline derivatives |
US4556640A (en) * | 1983-06-29 | 1985-12-03 | Miles Laboratories, Inc. | Stabilized test composition, device and method for the determination of peroxidatively active substances |
US4672029A (en) * | 1984-12-06 | 1987-06-09 | Eastman Kodak Company | Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes |
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