JPS5779899A - Kit for quantitative analysis of hydrogen peroxide - Google Patents
Kit for quantitative analysis of hydrogen peroxideInfo
- Publication number
- JPS5779899A JPS5779899A JP15431980A JP15431980A JPS5779899A JP S5779899 A JPS5779899 A JP S5779899A JP 15431980 A JP15431980 A JP 15431980A JP 15431980 A JP15431980 A JP 15431980A JP S5779899 A JPS5779899 A JP S5779899A
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen peroxide
- peroxidase
- enzymes
- solution containing
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To determine hydrogen peroxide, by using an enzymatic reagent obtained by freeze-drying an aqueous solution containing enzymes and 4-aminoantipyrine, in combination with a color-developing reagent composed of a buffer solution containing N-ethyl-N-[hydroxy-substituted sulfo-(lower alkyl)]-m- toluidine.
CONSTITUTION: An enzymatic reagent is prepared by freeze-drying a aqueous solution of enzymes and 4-aminoantipyrine. The enzymes are the combination of uricase and peroxidase, cholesterol-ester-hydrase, etc. and peroxidase, or phospholipase D, etc. and peroxidase, etc. Separately, a buffer solution containing N-ethyl-N-[hydroxy-substituted sulfo-(lower alkyl)]-m-toluidine is prepared. In the determination, the former enzymatic reagent is dissolved in the latter color- developing reagent, the solution is mixed with the specimen, and the amount of the hydrogen peroxide is determined from the absorbance of the mixture.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15431980A JPS5779899A (en) | 1980-10-31 | 1980-10-31 | Kit for quantitative analysis of hydrogen peroxide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15431980A JPS5779899A (en) | 1980-10-31 | 1980-10-31 | Kit for quantitative analysis of hydrogen peroxide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5779899A true JPS5779899A (en) | 1982-05-19 |
Family
ID=15581524
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP15431980A Pending JPS5779899A (en) | 1980-10-31 | 1980-10-31 | Kit for quantitative analysis of hydrogen peroxide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5779899A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59104553A (en) * | 1982-11-01 | 1984-06-16 | ベツクマン・インストルメンツ・インコ−ポレ−テツド | Method and reagent for determining peroxide and its precursor |
US4672029A (en) * | 1984-12-06 | 1987-06-09 | Eastman Kodak Company | Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes |
JP2012231751A (en) * | 2011-05-02 | 2012-11-29 | Niigata Prefecture | Method for obtaining yeast and method for producing liquor and food |
-
1980
- 1980-10-31 JP JP15431980A patent/JPS5779899A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59104553A (en) * | 1982-11-01 | 1984-06-16 | ベツクマン・インストルメンツ・インコ−ポレ−テツド | Method and reagent for determining peroxide and its precursor |
US4672029A (en) * | 1984-12-06 | 1987-06-09 | Eastman Kodak Company | Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes |
JP2012231751A (en) * | 2011-05-02 | 2012-11-29 | Niigata Prefecture | Method for obtaining yeast and method for producing liquor and food |
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