JPS5779899A - Kit for quantitative analysis of hydrogen peroxide - Google Patents

Kit for quantitative analysis of hydrogen peroxide

Info

Publication number
JPS5779899A
JPS5779899A JP15431980A JP15431980A JPS5779899A JP S5779899 A JPS5779899 A JP S5779899A JP 15431980 A JP15431980 A JP 15431980A JP 15431980 A JP15431980 A JP 15431980A JP S5779899 A JPS5779899 A JP S5779899A
Authority
JP
Japan
Prior art keywords
hydrogen peroxide
peroxidase
enzymes
solution containing
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15431980A
Other languages
Japanese (ja)
Inventor
Masanori Unoki
Sadao Shin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KAGAKU OYOBI KETSUSEIRIYOUHOU KENKYUSHO
Chemo Sero Therapeutic Research Institute Kaketsuken
Original Assignee
KAGAKU OYOBI KETSUSEIRIYOUHOU KENKYUSHO
Chemo Sero Therapeutic Research Institute Kaketsuken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KAGAKU OYOBI KETSUSEIRIYOUHOU KENKYUSHO, Chemo Sero Therapeutic Research Institute Kaketsuken filed Critical KAGAKU OYOBI KETSUSEIRIYOUHOU KENKYUSHO
Priority to JP15431980A priority Critical patent/JPS5779899A/en
Publication of JPS5779899A publication Critical patent/JPS5779899A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: To determine hydrogen peroxide, by using an enzymatic reagent obtained by freeze-drying an aqueous solution containing enzymes and 4-aminoantipyrine, in combination with a color-developing reagent composed of a buffer solution containing N-ethyl-N-[hydroxy-substituted sulfo-(lower alkyl)]-m- toluidine.
CONSTITUTION: An enzymatic reagent is prepared by freeze-drying a aqueous solution of enzymes and 4-aminoantipyrine. The enzymes are the combination of uricase and peroxidase, cholesterol-ester-hydrase, etc. and peroxidase, or phospholipase D, etc. and peroxidase, etc. Separately, a buffer solution containing N-ethyl-N-[hydroxy-substituted sulfo-(lower alkyl)]-m-toluidine is prepared. In the determination, the former enzymatic reagent is dissolved in the latter color- developing reagent, the solution is mixed with the specimen, and the amount of the hydrogen peroxide is determined from the absorbance of the mixture.
COPYRIGHT: (C)1982,JPO&Japio
JP15431980A 1980-10-31 1980-10-31 Kit for quantitative analysis of hydrogen peroxide Pending JPS5779899A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15431980A JPS5779899A (en) 1980-10-31 1980-10-31 Kit for quantitative analysis of hydrogen peroxide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15431980A JPS5779899A (en) 1980-10-31 1980-10-31 Kit for quantitative analysis of hydrogen peroxide

Publications (1)

Publication Number Publication Date
JPS5779899A true JPS5779899A (en) 1982-05-19

Family

ID=15581524

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15431980A Pending JPS5779899A (en) 1980-10-31 1980-10-31 Kit for quantitative analysis of hydrogen peroxide

Country Status (1)

Country Link
JP (1) JPS5779899A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59104553A (en) * 1982-11-01 1984-06-16 ベツクマン・インストルメンツ・インコ−ポレ−テツド Method and reagent for determining peroxide and its precursor
US4672029A (en) * 1984-12-06 1987-06-09 Eastman Kodak Company Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes
JP2012231751A (en) * 2011-05-02 2012-11-29 Niigata Prefecture Method for obtaining yeast and method for producing liquor and food

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59104553A (en) * 1982-11-01 1984-06-16 ベツクマン・インストルメンツ・インコ−ポレ−テツド Method and reagent for determining peroxide and its precursor
US4672029A (en) * 1984-12-06 1987-06-09 Eastman Kodak Company Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes
JP2012231751A (en) * 2011-05-02 2012-11-29 Niigata Prefecture Method for obtaining yeast and method for producing liquor and food

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