KR20210082556A - 반도체-기반 검출을 사용하는 고-처리율 서열분석용 시스템 및 디바이스 - Google Patents
반도체-기반 검출을 사용하는 고-처리율 서열분석용 시스템 및 디바이스 Download PDFInfo
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Abstract
Description
본 명세서에 포함된 도면 중 일부는 상이한 시야각으로부터 개시된 기술의 다양한 실시형태를 도시한다. 첨부된 설명 내용이 "상단", "하단" 또는 "측면" 관점과 같은 관점을 지칭할 수 있지만, 이러한 언급은 단지 설명이며, 달리 명시적으로 언급되지 않는 한 개시된 기술이 특정 공간적인 배향으로 구현되거나 사용되는 것을 암시하거나 요구하는 것은 아니다.
도 1은 일 실시형태에 따른 염기 콜링 시스템의 블록 다이어그램.
도 2는 도 1의 시스템에서 사용될 수 있는 시스템 제어기의 블록 다이어그램.
도 3은 각종 실시형태에서 사용될 수 있는 바이오센서의 단면도. 도 3의 바이오센서는 염기 콜링 주기 동안 하나를 초과하는 클러스터를 각각 보유할 수 있는 픽셀 면적을 갖는다(예를 들어, 픽셀 면적당 2개의 클러스터).
도 4는 각종 실시형태에서 사용될 수 있는 바이오센서의 단면도. 도 4의 바이오센서는 염기 콜링 주기 동안 하나를 초과하는 클러스터를 각각 보유할 수 있는 웰을 갖는다(예를 들어, 웰당 2개의 클러스터).
도 5A 및 도 5B는 일 실시형태에 따라서 공유된 센서(또는 픽셀)에 의해서 검출된 각각의 픽셀 신호를 사용한 밝은 클러스터 및 어두운 클러스터의 클러스터 쌍의 염기 콜링을 도시한 산포도.
도 6은 일 실시형태에 따라서 밝은 클러스터 및 어두운 클러스터의 클러스터 쌍으로부터의 강도 값에 의해서 생성된 16개의 분포를 도시한 산포도.
도 7A는 일 실시형태에 따른 하나의 염료 및 2개의 조명 단계 서열분석 프로토콜에 대한 염기 콜링 도식을 도시한 검출표.
도 7B는 일 실시형태에 따라서 밝은 클러스터 및 어두운 클러스터의 클러스터 쌍으로부터의 조합된 픽셀 신호를 16개의 빈 중 하나로 분류하기 위한 분류 도식을 나타낸 염기 콜링 표.
도 8은 일 실시형태에 따라서 픽셀 면적을 공유한 복수의 클러스터에 의해서 방출된 픽셀 신호의 분석에 의한 염기 콜링의 방법을 도시한 도면.
도 9는 일 실시형태에 따라서 바이오센서의 샘플 표면 상에 하나를 초과하는 클러스터를 갖는 픽셀 면적을 식별하고, 식별된 픽셀 면적에서 클러스터를 염기 콜링하는 방법이 제공된다.
도 10은 일 실시형태에 따라서 복수의 클러스터가 불균일하게 분포된 픽셀 면적을 갖는 샘플 표면의 평면도.
도 11A는 일 실시형태에 따라서 지배 웰 및 종속 웰을 포함하는, 픽셀 면적당 2개의 웰을 갖는 샘플 표면의 측면도.
도 11B는 도 11A의 샘플 표면의 평면도.
도 12A 및 도 12B는 샘플 표면의 픽셀 면적 위에 놓인 웰의 탈축 조명을 나타낸 도면.
도 12C는 일 실시형태에 따라서 도 12A 및 도 12B의 탈축 조명에 의해서 생성된 비대칭적으로 조명되는 웰 영역을 도시한 도면.
Claims (18)
- 염기 콜링용 디바이스(device for base calling)로서,
바이오센서를 보유하도록 구성된 용기로서, 상기 바이오센서는,
픽셀 면적을 포함하는 샘플 표면으로서, 클러스터가 상기 픽셀 면적 위에 불균일하게 분포되게끔 일련의 샘플링 사건 동안 복수의 클러스터를 보유하는, 상기 샘플 표면,
복수의 일련의 픽셀 신호를 생성시키도록 구성된 센서의 어레이로서, 상기 어레이는 N개의 능동 센서를 갖고, 상기 어레이 내의 센서는 상기 샘플 표면의 N개의 대응하는 픽셀 면적으로부터 일련의 샘플링 사건 동안 각각의 픽셀 신호를 생성시켜 복수의 일련의 픽셀 신호를 생성시키도록 상기 샘플 표면에 대해서 배치되는, 상기 센서의 어레이, 및
상기 복수의 일련의 픽셀 신호를 출력하는 통신 포트(communication port)를 갖는, 상기 용기; 및
상기 용기에 커플링된 신호 프로세서로서, 상기 복수의 일련의 픽셀 신호의 시간 순서 및 공간 분석을 실행하여 상기 N개의 능동 센서로부터의 상기 샘플 표면 상의 N + M개의 개별 클러스터(M은 양의 정수임)에 대응하는 조명의 패턴을 검출하고, 상기 N + M개의 개별 클러스터에 대한 상기 일련의 샘플링 사건의 결과를 분류하도록 구성되는, 상기 신호 프로세서
를 포함하는, 염기 콜링용 디바이스. - 제1항에 있어서,
상기 신호 프로세서는, 검출된 상기 조명 패턴을 사용하여 상기 N개의 능동 센서로부터 상기 샘플 표면 상에 상기 N + M개의 개별 클러스터를 위치시키는, 염기 콜링용 디바이스. - 염기 콜링용 디바이스로서,
바이오센서를 포함하되, 상기 바이오센서는, 픽셀 면적 및 픽셀 면적당 2개의 웰을 포함하며 상기 픽셀 면적 위에 놓인 웰의 어레이를 포함하는 샘플 표면을 갖고, 상기 픽셀 면적당 2개의 웰은 지배 웰(dominant well) 및 종속 웰(subordinate well)을 포함하고, 상기 지배 웰은 상기 종속 웰보다 픽셀 면적에서 더 넓은 단면을 갖는, 염기 콜링용 디바이스. - 제3항에 있어서, 상기 바이오센서는,
복수의 일련의 픽셀 신호를 생성시키도록 구성되는 센서 어레이로서, 상기 어레이는 N개의 능동 센서를 갖고, 상기 어레이 내의 센서는 상기 샘플 표면의 N개의 대응하는 픽셀 면적으로부터 일련의 샘플링 사건 동안 각각의 픽셀 신호를 생성시켜 복수의 일련의 픽셀 신호를 생성시키도록 상기 샘플 표면에 대해서 배치되는, 상기 센서의 어레이, 및
상기 복수의 일련의 픽셀 신호를 출력하는 통신 포트
를 포함하는, 염기 콜링용 디바이스. - 제3항에 있어서, 상기 2개의 웰은 상기 픽셀 면적의 중심에 대해서 상이한 오프셋을 갖는, 염기 콜링용 디바이스.
- 제5항에 있어서, 샘플링 사건 동안, 상기 픽셀 면적에 상기 2개의 웰로부터 상이한 양의 조명을 수신하는, 염기 콜링용 디바이스.
- 제6항에 있어서, 상기 2개의 웰 각각은 상기 샘플링 사건 동안 적어도 하나의 클러스터를 보유하는, 염기 콜링용 디바이스.
- 제7항에 있어서, 상기 샘플링 사건 동안, 상기 픽셀 면적에 상기 종속 웰 내의 어두운 클러스터(dim cluster)로부터 수신된 조명의 양보다 더 많은 상기 지배 웰 내의 밝은 클러스터(bright cluster)로부터의 조명의 양을 수신하는, 염기 콜링용 디바이스.
- 제8항에 있어서, 상기 바이오센서는 신호 프로세서에 커플링되되, 상기 신호 프로세서는 상기 복수의 일련의 픽셀 신호를 수신 및 처리하여, 상기 밝은 클러스터 및 어두운 클러스터에 대해서,
상기 샘플링 사건의 제1 조명 단계 동안 상기 픽셀 면적에 대응하는 센서에 의해서 생성된 제1 픽셀 신호를 적어도 4개의 빈(bin)에 맵핑(mapping)하고,
상기 샘플링 사건의 제2 조명 단계 동안 상기 센서에 의해서 생성된 제2 픽셀 신호를 적어도 4개의 빈에 맵핑하고,
상기 제1 픽셀 신호 및 상기 제2 픽셀 신호의 상기 맵핑을 논리적으로 조합하여 상기 밝은 클러스터 및 상기 어두운 클러스터에 존재하는 상기 염기를 식별하는 것
을 포함하는, 상기 N개의 능동 센서로부터의 N + M개의 클러스터에 존재하는 염기를 식별하도록 구성되는, 염기 콜링용 디바이스. - 염기 콜링용 디바이스로서,
픽셀 면적 및 상기 픽셀 면적 위에 놓인 웰의 어레이를 포함하는 샘플 표면을 갖는 바이오센서; 및
상기 바이오센서에 커플링되고, 일련의 샘플링 사건에서의 샘플링 사건에 대해서, 웰 각각을 탈축(off-axis) 조명으로 조명하는 것을 포함하여, 상기 일련의 샘플링 사건 동안 상기 픽셀 면적을 상이한 각의 조명으로 조명하여 상기 웰 각각에 비대칭적으로 조명되는 웰 영역을 생성시키는 조명 시스템
을 포함하는, 염기 콜링용 디바이스. - 제10항에 있어서, 상기 바이오센서는,
복수의 일련의 픽셀 신호를 생성시키도록 구성된 센서의 어레이로서, 상기 어레이는 N개의 능동 센서를 갖고, 상기 어레이 내의 센서는 상기 샘플 표면의 N개의 대응하는 픽셀 면적으로부터 일련의 샘플링 사건 동안 각각의 픽셀 신호를 생성시켜 상기 복수의 일련의 픽셀 신호를 생성시키도록 상기 샘플 표면에 대해서 배치되는, 상기 센서의 어레이 및
상기 복수의 일련의 픽셀 신호를 출력하는 통신 포트
를 포함하는, 염기 콜링용 디바이스. - 제10항에 있어서, 상기 비대칭적으로 조명되는 웰의 영역은 적어도 지배 웰 영역 및 종속 웰 영역을 포함하여, 상기 샘플링 사건 동안 상기 지배 웰 영역은 상기 종속 웰 영역보다 더 많이 조명되는, 염기 콜링용 디바이스.
- 제12항에 있어서, 상기 웰은 상기 샘플링 사건 동안 하나를 초과하는 클러스터를 보유하고, 상기 지배 웰 영역 및 상기 종속 웰 영역 각각은 클러스터를 포함하는, 염기 콜링용 디바이스.
- 제13항에 있어서, 상기 샘플링 사건 동안, 상기 웰 위에 놓인 픽셀 면적은 상기 종속 웰 영역 내의 어두운 클러스터로부터 수신된 조명의 양보다 더 많은 상기 지배 웰 영역 내의 밝은 클러스터로부터의 조명의 양을 수신하는, 염기 콜링용 디바이스.
- 제14항에 있어서, 상기 바이오센서는 신호 프로세서에 커플링되고, 상기 신호 프로세서는 상기 복수의 일련의 픽셀 신호를 수신 및 처리하여, 상기 밝은 클러스터 및 어두운 클러스터에 대해서,
상기 샘플링 사건의 제1 조명 단계 동안 상기 픽셀 면적에 대응하는 센서에 의해서 생성된 제1 픽셀 신호를 적어도 4개 빈에 맵핑하는 것,
상기 샘플링 사건의 제2 조명 단계 동안 상기 센서에 의해서 생성된 제2 픽셀 신호를 적어도 4개의 빈에 맵핑하는 것 및
상기 제1 픽셀 신호 및 상기 제2 픽셀 신호의 맵핑을 논리적으로 조합하여 상기 밝은 클러스터 및 상기 어두운 클러스터에 존재하는 상기 염기를 식별하는 것
을 포함하는, 상기 N개의 능동 센서로부터의 N + M개의 클러스터에 존재하는 염기를 식별하도록 구성되는, 염기 콜링용 디바이스. - 제10항에 있어서, 상기 탈축 조명은 45도 각인, 염기 콜링용 디바이스.
- 제10항에 있어서, 픽셀 면적당 하나의 웰이 위에 놓인, 염기 콜링용 디바이스.
- 제10항에 있어서, 픽셀 면적당 2개의 웰이 위에 놓인, 염기 콜링용 디바이스.
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL2020621B1 (en) | 2018-01-08 | 2019-07-15 | Illumina Inc | Multiplexing of an active sensor detector using structured illumination |
US11378544B2 (en) | 2018-01-08 | 2022-07-05 | Illumina, Inc. | High-throughput sequencing with semiconductor-based detection |
CN110832305A (zh) | 2018-01-08 | 2020-02-21 | 因美纳有限公司 | 利用基于半导体的检测的高通量测序系统和设备 |
CN112779152A (zh) * | 2020-12-30 | 2021-05-11 | 中国科学院长春光学精密机械与物理研究所 | 一种基因测序芯片及系统 |
CN112881432B (zh) * | 2021-01-12 | 2022-11-29 | 成都泓睿科技有限责任公司 | 一种带液玻璃瓶瓶口裂纹检测方法 |
KR20230149293A (ko) * | 2021-03-03 | 2023-10-26 | 일루미나, 인코포레이티드 | 픽셀당 다수의 반응 부위를 갖는 센서 |
US12112792B2 (en) * | 2021-08-10 | 2024-10-08 | Micron Technology, Inc. | Memory device for wafer-on-wafer formed memory and logic |
CN115184437B (zh) * | 2022-07-15 | 2024-02-02 | 山东云海国创云计算装备产业创新中心有限公司 | 一种基于risc-v的生物信号检测系统、方法及介质 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140274746A1 (en) * | 2013-03-15 | 2014-09-18 | Illumina, Inc. | Super resolution imaging |
WO2017045107A1 (en) * | 2015-09-14 | 2017-03-23 | Shenzhen Genorivision Technology Co. Ltd. | Biosensor |
WO2017184997A1 (en) * | 2016-04-22 | 2017-10-26 | Illumina, Inc. | Photonic stucture-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same |
KR20170135834A (ko) * | 2015-02-10 | 2017-12-08 | 일루미나, 인코포레이티드 | 세포 성분을 분석하기 위한 방법 및 조성물 |
Family Cites Families (121)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3856986A (en) | 1968-12-06 | 1974-12-24 | American Express Invest | Scanned holography systems using temporal modulation |
US4213706A (en) | 1977-08-19 | 1980-07-22 | The University Of Arizona Foundation | Background compensating interferometer |
CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
US6090592A (en) | 1994-08-03 | 2000-07-18 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid on supports |
US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
US5761085A (en) | 1996-11-12 | 1998-06-02 | The United States Of America As Represented By The Secretary Of The Navy | Method for monitoring environmental parameters at network sites |
JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
US20020055100A1 (en) | 1997-04-01 | 2002-05-09 | Kawashima Eric H. | Method of nucleic acid sequencing |
AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
US6188478B1 (en) | 1998-10-21 | 2001-02-13 | Philips Electronics North America Corporation | Method and apparatus for film-thickness measurements |
CN1292227A (zh) | 1999-10-09 | 2001-04-25 | 胡嘉鹏 | 一种氨基酸麦香奶茶的配制及工艺 |
WO2002004680A2 (en) | 2000-07-07 | 2002-01-17 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
EP1354064A2 (en) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
US7274446B2 (en) | 2001-04-07 | 2007-09-25 | Carl Zeiss Jena Gmbh | Method and arrangement for the deep resolved optical recording of a sample |
US7151246B2 (en) | 2001-07-06 | 2006-12-19 | Palantyr Research, Llc | Imaging system and methodology |
US6975659B2 (en) | 2001-09-10 | 2005-12-13 | Fuji Photo Film Co., Ltd. | Laser diode array, laser device, wave-coupling laser source, and exposure device |
JP5048899B2 (ja) | 2001-09-28 | 2012-10-17 | オリンパス株式会社 | 顕微鏡 |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
US6947127B2 (en) | 2001-12-10 | 2005-09-20 | Carl Zeiss Jena Gmbh | Arrangement for the optical capture of excited and/or back scattered light beam in a sample |
US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
KR100489431B1 (ko) | 2002-06-28 | 2005-05-12 | 학교법인연세대학교 | 스캔식 위상천이 3차원 형상측정 장치 및 측정 방법 |
US6641658B1 (en) | 2002-07-03 | 2003-11-04 | United States Gypsum Company | Rapid setting cementitious composition |
EP3363809B1 (en) | 2002-08-23 | 2020-04-08 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
US7595883B1 (en) * | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
JP2005055219A (ja) | 2003-08-07 | 2005-03-03 | Juki Corp | マイクロプレートリーダ |
JP2005080181A (ja) | 2003-09-03 | 2005-03-24 | Nikon Corp | 画像読み取り装置および画像読み取りプログラム |
GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
WO2005043197A2 (en) | 2003-10-28 | 2005-05-12 | University Of Maryland, Baltimore | Spatial light modulator apparatus and method |
US20110059865A1 (en) | 2004-01-07 | 2011-03-10 | Mark Edward Brennan Smith | Modified Molecular Arrays |
US20050221351A1 (en) | 2004-04-06 | 2005-10-06 | Affymetrix, Inc. | Methods and devices for microarray image analysis |
US7385693B2 (en) | 2004-06-21 | 2008-06-10 | Olympus Corporation | Microscope apparatus |
US7302146B2 (en) | 2004-09-17 | 2007-11-27 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
WO2006109448A1 (ja) | 2005-03-30 | 2006-10-19 | Nikon Corporation | 画像作成方法および顕微鏡装置 |
JP4990886B2 (ja) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | 改良ポリメラーゼ |
EP3257949A1 (en) | 2005-06-15 | 2017-12-20 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
GB0517097D0 (en) | 2005-08-19 | 2005-09-28 | Solexa Ltd | Modified nucleosides and nucleotides and uses thereof |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
CN101466998B (zh) | 2005-11-09 | 2015-09-16 | 几何信息学股份有限公司 | 三维绝对坐标表面成像的方法和装置 |
JP2007199397A (ja) | 2006-01-26 | 2007-08-09 | Nikon Corp | 顕微鏡装置 |
US7995202B2 (en) * | 2006-02-13 | 2011-08-09 | Pacific Biosciences Of California, Inc. | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
WO2007107710A1 (en) | 2006-03-17 | 2007-09-27 | Solexa Limited | Isothermal methods for creating clonal single molecule arrays |
EP3722409A1 (en) | 2006-03-31 | 2020-10-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
US8178360B2 (en) | 2006-05-18 | 2012-05-15 | Illumina Cambridge Limited | Dye compounds and the use of their labelled conjugates |
US7803609B2 (en) | 2006-07-21 | 2010-09-28 | Affymetrix, Inc. | System, method, and product for generating patterned illumination |
DE102006044229B4 (de) | 2006-09-20 | 2023-09-28 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zur Bildverarbeitung mit höheren Harmonischen eines Beleuchtungsgitters |
DE102006047912A1 (de) | 2006-10-06 | 2008-04-10 | Carl Zeiss Microimaging Gmbh | Verfahren und Anordnung zur parallelisierten mikroskopischen Bildgebung |
WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
US20080242560A1 (en) | 2006-11-21 | 2008-10-02 | Gunderson Kevin L | Methods for generating amplified nucleic acid arrays |
FR2908888B1 (fr) | 2006-11-21 | 2012-08-03 | Centre Nat Rech Scient | Dispositif pour la detection exaltee de l'emission d'une particule cible |
EP2093601B1 (en) | 2006-12-12 | 2013-03-13 | Nikon Corporation | Microscope device and image processing method |
GB2457851B (en) | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
CN101652780B (zh) * | 2007-01-26 | 2012-10-03 | 伊鲁米那股份有限公司 | 核酸测序系统以及方法 |
CA2687062C (en) | 2007-05-10 | 2016-04-12 | Pacific Biosciences Of California, Inc. | Methods and systems for analyzing fluorescent materials with reduced autofluorescence |
WO2009009081A2 (en) | 2007-07-10 | 2009-01-15 | Massachusetts Institute Of Technology | Tomographic phase microscopy |
US8222040B2 (en) | 2007-08-28 | 2012-07-17 | Lightspeed Genomics, Inc. | Nucleic acid sequencing by selective excitation of microparticles |
US8759077B2 (en) | 2007-08-28 | 2014-06-24 | Lightspeed Genomics, Inc. | Apparatus for selective excitation of microparticles |
US8509879B2 (en) | 2007-11-06 | 2013-08-13 | The Regents Of The University Of California | Apparatus and method for widefield functional imaging (WiFI) using integrated structured illumination and laser speckle imaging |
US20090219607A1 (en) | 2008-01-17 | 2009-09-03 | Baylor College Of Medicine | Method and apparatus for enhanced resolution microscopy of living biological nanostructures |
DE102008009216A1 (de) | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
US8045181B2 (en) | 2009-05-21 | 2011-10-25 | General Electric Company | Inspection system and method with multi-image phase shift analysis |
US8416400B2 (en) | 2009-06-03 | 2013-04-09 | California Institute Of Technology | Wavefront imaging sensor |
US8502867B2 (en) | 2010-03-19 | 2013-08-06 | Lightspeed Genomics, Inc. | Synthetic aperture optics imaging method using minimum selective excitation patterns |
US9465228B2 (en) | 2010-03-19 | 2016-10-11 | Optical Biosystems, Inc. | Illumination apparatus optimized for synthetic aperture optics imaging using minimum selective excitation patterns |
WO2012031234A2 (en) * | 2010-09-03 | 2012-03-08 | Life Technologies Corporation | Methods, systems and apparatus for sequencing |
EP2632593B1 (en) * | 2010-10-27 | 2021-09-29 | Illumina, Inc. | Flow cells for biological or chemical analysis |
US9387476B2 (en) * | 2010-10-27 | 2016-07-12 | Illumina, Inc. | Flow cells for biological or chemical analysis |
DE102010062341B4 (de) | 2010-12-02 | 2023-05-17 | Carl Zeiss Microscopy Gmbh | Vorrichtung zur Erhöhung der Tiefendiskriminierung optisch abbildender Systeme |
WO2012118436A1 (en) | 2011-03-01 | 2012-09-07 | Applied Precision, Inc. | Systems and methods for illumination phase control in fluorescence microscopy |
CN103534627A (zh) | 2011-03-03 | 2014-01-22 | 加州理工学院 | 光导像素 |
WO2012122418A1 (en) | 2011-03-08 | 2012-09-13 | Lightspeed Genomics, Inc. | Self-assembling high density ordered patterned biomolecule array and method for making and using the same |
KR101252938B1 (ko) | 2011-04-08 | 2013-04-12 | 한국과학기술원 | 초고해상도 현미경 시스템 및 그 시스템을 이용한 영상 획득 방법 |
US8817362B2 (en) | 2011-04-29 | 2014-08-26 | Lightspeed Genomics, Inc. | Modular pattern illumination and light beam multiplexing for selective excitation of microparticles |
WO2013035009A1 (en) | 2011-09-06 | 2013-03-14 | Koninklijke Philips Electronics N.V. | Optical biosensor with a plurality of sensors regions |
CN104160391A (zh) | 2011-09-16 | 2014-11-19 | 考利达基因组股份有限公司 | 确定异质样本的基因组中的变异 |
EP2768972B2 (en) | 2011-09-23 | 2020-07-22 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
EP2776962A4 (en) | 2011-11-07 | 2015-12-02 | Ingenuity Systems Inc | METHODS AND SYSTEMS FOR IDENTIFICATION OF CAUSAL GENOMIC VARIANTS |
JP5900515B2 (ja) | 2012-01-18 | 2016-04-06 | 株式会社ニコン | 構造化照明装置、構造化照明顕微鏡装置、構造化照明方法 |
EP2831220B1 (en) * | 2012-03-29 | 2020-10-21 | Mitegen, LLC | Improvements to microplates |
US8906320B1 (en) | 2012-04-16 | 2014-12-09 | Illumina, Inc. | Biosensors for biological or chemical analysis and systems and methods for same |
EP2855707B1 (en) | 2012-05-31 | 2017-07-12 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of dna |
US8969781B2 (en) * | 2012-06-28 | 2015-03-03 | Board Of Regents, The University Of Texas System | Integrated optical biosensor array including charge injection circuit and quantizer circuit |
US20140143188A1 (en) | 2012-11-16 | 2014-05-22 | Genformatic, Llc | Method of machine learning, employing bayesian latent class inference: combining multiple genomic feature detection algorithms to produce an integrated genomic feature set with specificity, sensitivity and accuracy |
US20140235456A1 (en) | 2012-12-17 | 2014-08-21 | Virginia Tech Intellectual Properties, Inc. | Methods and Compositions for Identifying Global Microsatellite Instability and for Characterizing Informative Microsatellite Loci |
KR101460066B1 (ko) * | 2012-12-21 | 2014-11-11 | 한양대학교 에리카산학협력단 | 대면적 기판에서의 나노웰 어레이 바이오센서 제조방법 |
JP5426751B2 (ja) | 2012-12-28 | 2014-02-26 | 未来工業株式会社 | 配線・配管材支持具 |
NZ739844A (en) | 2013-01-11 | 2020-07-31 | Regeneron Pharma | Systems and devices for sample handling |
EP2801854B1 (en) | 2013-05-10 | 2017-07-19 | Ruprecht-Karls-Universität Heidelberg | Method and apparatus for combination of localization microscopy and structured illumination microscopy |
CA2930832A1 (en) | 2013-11-17 | 2015-05-21 | Quantum-Si Incorporated | Optical system and assay chip for probing, detecting and analyzing molecules |
PT3077943T (pt) | 2013-12-03 | 2020-08-21 | Illumina Inc | Métodos e sistemas para analisar dados de imagem |
BR122020010030B1 (pt) * | 2013-12-10 | 2022-02-01 | Illumina, Inc | Biossensores para análise química ou biológica |
US9430830B2 (en) * | 2014-01-30 | 2016-08-30 | Case Western Reserve University | Spatially aware cell cluster (SPACCL) graphs |
WO2015167492A1 (en) | 2014-04-30 | 2015-11-05 | Hewlett-Packard Development Company, L.P. | Imaging appratus and methods using diffraction-based illumination |
CN106462670B (zh) | 2014-05-12 | 2020-04-10 | 豪夫迈·罗氏有限公司 | 超深度测序中的罕见变体召集 |
US10174363B2 (en) * | 2015-05-20 | 2019-01-08 | Quantum-Si Incorporated | Methods for nucleic acid sequencing |
CN106796175B (zh) | 2014-08-08 | 2021-01-05 | 宽腾矽公司 | 用于探测、检测和分析分子的光学系统和检测芯片 |
KR101569515B1 (ko) * | 2014-08-13 | 2015-11-27 | (주)옵토레인 | 측광형 발광소자가 내장된 바이오칩 및 그 제조방법 |
CN107076729A (zh) | 2014-10-16 | 2017-08-18 | 康希尔公司 | 变异体调用器 |
EP3271762B1 (en) | 2015-03-16 | 2023-01-04 | Pacific Biosciences of California, Inc. | Analytical system comprising integrated devices and systems for free-space optical coupling |
CN107533005B (zh) | 2015-04-22 | 2020-07-24 | 深圳源光科技有限公司 | 生物感测器 |
CN105043300A (zh) | 2015-05-27 | 2015-11-11 | 东莞市盟拓光电科技有限公司 | 可从多方位对被测物同时投影的三维测量系统 |
CA2894317C (en) | 2015-06-15 | 2023-08-15 | Deep Genomics Incorporated | Systems and methods for classifying, prioritizing and interpreting genetic variants and therapies using a deep neural network |
TWI794145B (zh) | 2015-10-28 | 2023-03-01 | 美商加州太平洋生物科學公司 | 包含整合性帶通濾波器之光學裝置陣列 |
US10629291B2 (en) | 2016-03-10 | 2020-04-21 | Koninklijke Philips N.V. | Antibiotic resistance causation identification |
MX2018014856A (es) * | 2016-06-01 | 2019-03-07 | Quantum Si Inc | Llamador de pulso y llamador de base. |
WO2017214320A1 (en) | 2016-06-07 | 2017-12-14 | Edico Genome, Corp. | Bioinformatics systems, apparatus, and methods for performing secondary and/or tertiary processing |
DE102016007839A1 (de) | 2016-06-28 | 2017-12-28 | Horst Wochnowski | Hochauflösendes Verfahren zur Mikroskopie und Nanostrukturierung von Substratoberflächen basierend auf dem SIM-Verfahren (Structured Illumination Microscopy) |
CN106770147B (zh) | 2017-03-15 | 2019-07-19 | 北京大学 | 一种结构光照明超分辨显微成像方法 |
KR102676067B1 (ko) | 2017-03-17 | 2024-06-18 | 퍼시픽 바이오사이언스 오브 캘리포니아, 인크. | 시퀀싱 및 고 해상도 이미징 |
US10878273B2 (en) | 2017-07-06 | 2020-12-29 | Texas Instruments Incorporated | Dynamic quantization for deep neural network inference system and method |
NL2020758B1 (en) | 2018-01-08 | 2019-07-15 | Illumina Inc | High-throughput sequencing with semiconductor-based detection |
NL2020621B1 (en) | 2018-01-08 | 2019-07-15 | Illumina Inc | Multiplexing of an active sensor detector using structured illumination |
US11378544B2 (en) | 2018-01-08 | 2022-07-05 | Illumina, Inc. | High-throughput sequencing with semiconductor-based detection |
CN110832305A (zh) * | 2018-01-08 | 2020-02-21 | 因美纳有限公司 | 利用基于半导体的检测的高通量测序系统和设备 |
US11676685B2 (en) | 2019-03-21 | 2023-06-13 | Illumina, Inc. | Artificial intelligence-based quality scoring |
US11423306B2 (en) | 2019-05-16 | 2022-08-23 | Illumina, Inc. | Systems and devices for characterization and performance analysis of pixel-based sequencing |
-
2019
- 2019-01-07 CN CN201980003276.6A patent/CN110832305A/zh active Pending
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- 2021-03-18 IL IL281644A patent/IL281644A/en unknown
- 2021-05-02 IL IL282848A patent/IL282848B2/en unknown
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-
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- 2023-01-19 US US18/157,036 patent/US20230158494A1/en active Pending
- 2023-02-24 ZA ZA2023/02485A patent/ZA202302485B/en unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140274746A1 (en) * | 2013-03-15 | 2014-09-18 | Illumina, Inc. | Super resolution imaging |
KR20170135834A (ko) * | 2015-02-10 | 2017-12-08 | 일루미나, 인코포레이티드 | 세포 성분을 분석하기 위한 방법 및 조성물 |
WO2017045107A1 (en) * | 2015-09-14 | 2017-03-23 | Shenzhen Genorivision Technology Co. Ltd. | Biosensor |
WO2017184997A1 (en) * | 2016-04-22 | 2017-10-26 | Illumina, Inc. | Photonic stucture-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same |
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