KR20030013462A - ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 - Google Patents
ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 Download PDFInfo
- Publication number
- KR20030013462A KR20030013462A KR1020027017579A KR20027017579A KR20030013462A KR 20030013462 A KR20030013462 A KR 20030013462A KR 1020027017579 A KR1020027017579 A KR 1020027017579A KR 20027017579 A KR20027017579 A KR 20027017579A KR 20030013462 A KR20030013462 A KR 20030013462A
- Authority
- KR
- South Korea
- Prior art keywords
- virus
- measles virus
- canine
- genome
- cdv
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000002299 complementary DNA Substances 0.000 title claims abstract description 50
- 241000712083 Canine morbillivirus Species 0.000 title description 138
- 241000700605 Viruses Species 0.000 claims abstract description 191
- 241000712079 Measles morbillivirus Species 0.000 claims abstract description 169
- 238000000034 method Methods 0.000 claims abstract description 95
- 241000282465 Canis Species 0.000 claims abstract description 89
- 239000000203 mixture Substances 0.000 claims abstract description 71
- 239000002773 nucleotide Substances 0.000 claims abstract description 51
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 51
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 51
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 51
- 239000002157 polynucleotide Substances 0.000 claims abstract description 51
- 230000002238 attenuated effect Effects 0.000 claims abstract description 48
- 230000002163 immunogen Effects 0.000 claims abstract description 43
- 208000015181 infectious disease Diseases 0.000 claims abstract description 39
- 241001493065 dsRNA viruses Species 0.000 claims abstract description 17
- 230000002458 infectious effect Effects 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 210000004027 cell Anatomy 0.000 claims description 164
- 108090000623 proteins and genes Proteins 0.000 claims description 130
- 241000282472 Canis lupus familiaris Species 0.000 claims description 84
- 238000001890 transfection Methods 0.000 claims description 62
- 102000004169 proteins and genes Human genes 0.000 claims description 59
- 239000013612 plasmid Substances 0.000 claims description 52
- 239000013598 vector Substances 0.000 claims description 44
- 238000013518 transcription Methods 0.000 claims description 40
- 230000035897 transcription Effects 0.000 claims description 40
- 239000013604 expression vector Substances 0.000 claims description 37
- 101710137500 T7 RNA polymerase Proteins 0.000 claims description 33
- 229910052757 nitrogen Inorganic materials 0.000 claims description 33
- 229910052698 phosphorus Inorganic materials 0.000 claims description 32
- 241001465754 Metazoa Species 0.000 claims description 30
- 108091007433 antigens Proteins 0.000 claims description 29
- 150000007523 nucleic acids Chemical class 0.000 claims description 28
- 239000000427 antigen Substances 0.000 claims description 27
- 102000036639 antigens Human genes 0.000 claims description 27
- 230000035772 mutation Effects 0.000 claims description 26
- 102000039446 nucleic acids Human genes 0.000 claims description 26
- 108020004707 nucleic acids Proteins 0.000 claims description 26
- 230000010076 replication Effects 0.000 claims description 25
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 19
- 241000701931 Canine parvovirus Species 0.000 claims description 18
- 230000002103 transcriptional effect Effects 0.000 claims description 15
- 108091026890 Coding region Proteins 0.000 claims description 13
- 244000052769 pathogen Species 0.000 claims description 13
- 239000003937 drug carrier Substances 0.000 claims description 7
- 241000282326 Felis catus Species 0.000 claims description 6
- 210000003292 kidney cell Anatomy 0.000 claims description 6
- 241000283690 Bos taurus Species 0.000 claims description 5
- 108091092724 Noncoding DNA Proteins 0.000 claims description 5
- 230000001717 pathogenic effect Effects 0.000 claims description 5
- 241000711386 Mumps virus Species 0.000 claims description 4
- 241000711798 Rabies lyssavirus Species 0.000 claims description 4
- 230000001681 protective effect Effects 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 210000003705 ribosome Anatomy 0.000 claims description 4
- 230000009466 transformation Effects 0.000 claims description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 241001353878 Canine parainfluenza virus Species 0.000 claims description 3
- 241000283086 Equidae Species 0.000 claims description 3
- 241000282887 Suidae Species 0.000 claims description 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims description 3
- 230000007547 defect Effects 0.000 claims description 3
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 3
- 210000005260 human cell Anatomy 0.000 claims description 3
- 230000003053 immunization Effects 0.000 claims description 3
- 241000701101 Canine adenovirus 1 Species 0.000 claims description 2
- 241000701114 Canine adenovirus 2 Species 0.000 claims description 2
- 241000711506 Canine coronavirus Species 0.000 claims description 2
- 241001032492 Canine parvovirus 2 Species 0.000 claims description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 2
- 208000005647 Mumps Diseases 0.000 claims description 2
- 201000005296 lung carcinoma Diseases 0.000 claims description 2
- 208000010805 mumps infectious disease Diseases 0.000 claims description 2
- 241000724205 Rice stripe tenuivirus Species 0.000 claims 1
- 239000000556 agonist Substances 0.000 claims 1
- 210000002308 embryonic cell Anatomy 0.000 claims 1
- 238000003306 harvesting Methods 0.000 claims 1
- 201000003911 head and neck carcinoma Diseases 0.000 claims 1
- 238000010361 transduction Methods 0.000 claims 1
- 230000026683 transduction Effects 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 abstract description 13
- 238000003780 insertion Methods 0.000 abstract description 7
- 230000037431 insertion Effects 0.000 abstract description 7
- 238000012217 deletion Methods 0.000 abstract description 3
- 230000037430 deletion Effects 0.000 abstract description 3
- 230000008707 rearrangement Effects 0.000 abstract description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 56
- 235000018102 proteins Nutrition 0.000 description 54
- 108020004414 DNA Proteins 0.000 description 42
- 230000000694 effects Effects 0.000 description 38
- 239000012634 fragment Substances 0.000 description 37
- 230000003612 virological effect Effects 0.000 description 33
- 230000014509 gene expression Effects 0.000 description 30
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 28
- 108060001084 Luciferase Proteins 0.000 description 28
- 108090000765 processed proteins & peptides Proteins 0.000 description 24
- 235000001014 amino acid Nutrition 0.000 description 23
- 238000002474 experimental method Methods 0.000 description 21
- 229960005486 vaccine Drugs 0.000 description 21
- 150000001413 amino acids Chemical class 0.000 description 20
- 102000004196 processed proteins & peptides Human genes 0.000 description 18
- 206010072219 Mevalonic aciduria Diseases 0.000 description 17
- 125000003275 alpha amino acid group Chemical group 0.000 description 17
- 102000053642 Catalytic RNA Human genes 0.000 description 16
- 108090000994 Catalytic RNA Proteins 0.000 description 16
- 101710081079 Minor spike protein H Proteins 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 16
- 108091092562 ribozyme Proteins 0.000 description 16
- 241001183012 Modified Vaccinia Ankara virus Species 0.000 description 15
- 239000013613 expression plasmid Substances 0.000 description 15
- 239000005089 Luciferase Substances 0.000 description 14
- 239000000284 extract Substances 0.000 description 14
- 239000001506 calcium phosphate Substances 0.000 description 13
- 229960001714 calcium phosphate Drugs 0.000 description 13
- 229910000389 calcium phosphate Inorganic materials 0.000 description 13
- 235000011010 calcium phosphates Nutrition 0.000 description 13
- 230000000977 initiatory effect Effects 0.000 description 13
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 13
- 238000010367 cloning Methods 0.000 description 12
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 12
- 239000013642 negative control Substances 0.000 description 12
- 229920001184 polypeptide Polymers 0.000 description 11
- 101150093578 VP2 gene Proteins 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 description 9
- 101150084044 P gene Proteins 0.000 description 9
- 238000012408 PCR amplification Methods 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 9
- 238000011534 incubation Methods 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 210000003501 vero cell Anatomy 0.000 description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 8
- 108020004705 Codon Proteins 0.000 description 8
- 230000000295 complement effect Effects 0.000 description 8
- 230000002950 deficient Effects 0.000 description 8
- 239000012091 fetal bovine serum Substances 0.000 description 8
- 108020004999 messenger RNA Proteins 0.000 description 8
- 239000013600 plasmid vector Substances 0.000 description 8
- 230000006798 recombination Effects 0.000 description 8
- 238000005215 recombination Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 241000282421 Canidae Species 0.000 description 7
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 7
- 101710141454 Nucleoprotein Proteins 0.000 description 7
- 239000012124 Opti-MEM Substances 0.000 description 7
- 101710181008 P protein Proteins 0.000 description 7
- 101710177166 Phosphoprotein Proteins 0.000 description 7
- 230000003321 amplification Effects 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 230000001976 improved effect Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 7
- 108091008146 restriction endonucleases Proteins 0.000 description 7
- 238000012163 sequencing technique Methods 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 108091029795 Intergenic region Proteins 0.000 description 6
- 108090001074 Nucleocapsid Proteins Proteins 0.000 description 6
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 241000700618 Vaccinia virus Species 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 239000008194 pharmaceutical composition Substances 0.000 description 6
- 230000000069 prophylactic effect Effects 0.000 description 6
- 230000001225 therapeutic effect Effects 0.000 description 6
- 241000282693 Cercopithecidae Species 0.000 description 5
- 241000700721 Hepatitis B virus Species 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 108700008625 Reporter Genes Proteins 0.000 description 5
- 239000002671 adjuvant Substances 0.000 description 5
- 230000037429 base substitution Effects 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 101150055766 cat gene Proteins 0.000 description 5
- 230000028993 immune response Effects 0.000 description 5
- 239000002502 liposome Substances 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 208000011580 syndromic disease Diseases 0.000 description 5
- 238000001262 western blot Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 102000009016 Cholera Toxin Human genes 0.000 description 4
- 108010049048 Cholera Toxin Proteins 0.000 description 4
- 238000001712 DNA sequencing Methods 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 241000589902 Leptospira Species 0.000 description 4
- 241000125945 Protoparvovirus Species 0.000 description 4
- 206010037742 Rabies Diseases 0.000 description 4
- 241000725643 Respiratory syncytial virus Species 0.000 description 4
- 238000010171 animal model Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 239000013553 cell monolayer Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 238000001415 gene therapy Methods 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000002703 mutagenesis Methods 0.000 description 4
- 231100000350 mutagenesis Toxicity 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 230000000644 propagated effect Effects 0.000 description 4
- 230000003362 replicative effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000241 respiratory effect Effects 0.000 description 4
- 230000035939 shock Effects 0.000 description 4
- 239000002356 single layer Substances 0.000 description 4
- 230000008685 targeting Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 239000012096 transfection reagent Substances 0.000 description 4
- 230000009385 viral infection Effects 0.000 description 4
- 241000711404 Avian avulavirus 1 Species 0.000 description 3
- 241000588807 Bordetella Species 0.000 description 3
- 241000589969 Borreliella burgdorferi Species 0.000 description 3
- 108091035707 Consensus sequence Proteins 0.000 description 3
- 241000711573 Coronaviridae Species 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 208000016192 Demyelinating disease Diseases 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101150062031 L gene Proteins 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 241000282339 Mustela Species 0.000 description 3
- 108010076039 Polyproteins Proteins 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 108010067390 Viral Proteins Proteins 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 238000000376 autoradiography Methods 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000008827 biological function Effects 0.000 description 3
- 206010006451 bronchitis Diseases 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000012252 genetic analysis Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 238000001638 lipofection Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- -1 ny Species 0.000 description 3
- 244000062645 predators Species 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000002741 site-directed mutagenesis Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 229960005367 tetanus antitoxin Drugs 0.000 description 3
- 230000001052 transient effect Effects 0.000 description 3
- 230000010474 transient expression Effects 0.000 description 3
- 230000014616 translation Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- DRHZYJAUECRAJM-DWSYSWFDSA-N (2s,3s,4s,5r,6r)-6-[[(3s,4s,4ar,6ar,6bs,8r,8ar,12as,14ar,14br)-8a-[(2s,3r,4s,5r,6r)-3-[(2s,3r,4s,5r,6s)-5-[(2s,3r,4s,5r)-4-[(2s,3r,4r)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-3,5-dihydroxyoxan-2-yl]oxy-3,4-dihydroxy-6-methyloxan-2-yl]oxy-5-[(3s,5s, Chemical compound O([C@H]1[C@H](O)[C@H](O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O1)O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@H]5CC(C)(C)CC[C@@]5([C@@H](C[C@@]4(C)[C@]3(C)CC[C@H]2[C@@]1(C=O)C)O)C(=O)O[C@@H]1O[C@H](C)[C@@H]([C@@H]([C@H]1O[C@H]1[C@@H]([C@H](O)[C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@](O)(CO)CO3)O)[C@H](O)CO2)O)[C@H](C)O1)O)O)OC(=O)C[C@@H](O)C[C@H](OC(=O)C[C@@H](O)C[C@@H]([C@@H](C)CC)O[C@H]1[C@@H]([C@@H](O)[C@H](CO)O1)O)[C@@H](C)CC)C(O)=O)[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O DRHZYJAUECRAJM-DWSYSWFDSA-N 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 102000002659 Amyloid Precursor Protein Secretases Human genes 0.000 description 2
- 108010043324 Amyloid Precursor Protein Secretases Proteins 0.000 description 2
- 230000007082 Aβ accumulation Effects 0.000 description 2
- 241000712005 Bovine respirovirus 3 Species 0.000 description 2
- 101150047856 Cav2 gene Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 208000000655 Distemper Diseases 0.000 description 2
- 241000701867 Enterobacteria phage T7 Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 208000005577 Gastroenteritis Diseases 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 241000606831 Histophilus somni Species 0.000 description 2
- 241000712003 Human respirovirus 3 Species 0.000 description 2
- 102100034349 Integrase Human genes 0.000 description 2
- 102000013462 Interleukin-12 Human genes 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- 102000015696 Interleukins Human genes 0.000 description 2
- 108010063738 Interleukins Proteins 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 241001293418 Mannheimia haemolytica Species 0.000 description 2
- 241000282342 Martes americana Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 2
- 241000588650 Neisseria meningitidis Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 241000772415 Neovison vison Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 208000037581 Persistent Infection Diseases 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 206010035664 Pneumonia Diseases 0.000 description 2
- 241000282330 Procyon lotor Species 0.000 description 2
- 241000702670 Rotavirus Species 0.000 description 2
- 241000282849 Ruminantia Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 241000193998 Streptococcus pneumoniae Species 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 241000224526 Trichomonas Species 0.000 description 2
- 108700005077 Viral Genes Proteins 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 239000013566 allergen Substances 0.000 description 2
- NWMHDZMRVUOQGL-CZEIJOLGSA-N almurtide Chemical compound OC(=O)CC[C@H](C(N)=O)NC(=O)[C@H](C)NC(=O)CO[C@@H]([C@H](O)[C@H](O)CO)[C@@H](NC(C)=O)C=O NWMHDZMRVUOQGL-CZEIJOLGSA-N 0.000 description 2
- DZHSAHHDTRWUTF-SIQRNXPUSA-N amyloid-beta polypeptide 42 Chemical compound C([C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O)[C@@H](C)CC)C(C)C)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C(C)C)C1=CC=CC=C1 DZHSAHHDTRWUTF-SIQRNXPUSA-N 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 230000027645 antigenic variation Effects 0.000 description 2
- 206010003230 arteritis Diseases 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000007975 buffered saline Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 210000003837 chick embryo Anatomy 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 229960005091 chloramphenicol Drugs 0.000 description 2
- XFIOKOXROGCUQX-UHFFFAOYSA-N chloroform;guanidine;phenol Chemical compound NC(N)=N.ClC(Cl)Cl.OC1=CC=CC=C1 XFIOKOXROGCUQX-UHFFFAOYSA-N 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 230000001524 infective effect Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 210000003563 lymphoid tissue Anatomy 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 238000000329 molecular dynamics simulation Methods 0.000 description 2
- 238000007857 nested PCR Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 238000002205 phenol-chloroform extraction Methods 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 230000029812 viral genome replication Effects 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000606750 Actinobacillus Species 0.000 description 1
- 241000606748 Actinobacillus pleuropneumoniae Species 0.000 description 1
- KXEVYGKATAMXJJ-ACZMJKKPSA-N Ala-Glu-Asp Chemical compound C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O KXEVYGKATAMXJJ-ACZMJKKPSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 102000009091 Amyloidogenic Proteins Human genes 0.000 description 1
- 108010048112 Amyloidogenic Proteins Proteins 0.000 description 1
- 241000272478 Aquila Species 0.000 description 1
- 241000244186 Ascaris Species 0.000 description 1
- XEDQMTWEYFBOIK-ACZMJKKPSA-N Asp-Ala-Glu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O XEDQMTWEYFBOIK-ACZMJKKPSA-N 0.000 description 1
- KGHLGJAXYSVNJP-WHFBIAKZSA-N Asp-Ser-Gly Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O KGHLGJAXYSVNJP-WHFBIAKZSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000537222 Betabaculovirus Species 0.000 description 1
- 241000335423 Blastomyces Species 0.000 description 1
- 241000588832 Bordetella pertussis Species 0.000 description 1
- 241000711895 Bovine orthopneumovirus Species 0.000 description 1
- 206010006448 Bronchiolitis Diseases 0.000 description 1
- 206010006811 Bursitis Diseases 0.000 description 1
- 101150111062 C gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000589876 Campylobacter Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 102000012406 Carcinoembryonic Antigen Human genes 0.000 description 1
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 241001647372 Chlamydia pneumoniae Species 0.000 description 1
- 241001647378 Chlamydia psittaci Species 0.000 description 1
- 241000606153 Chlamydia trachomatis Species 0.000 description 1
- 241000193449 Clostridium tetani Species 0.000 description 1
- 241000737241 Cocos Species 0.000 description 1
- 241000186227 Corynebacterium diphtheriae Species 0.000 description 1
- 241001481833 Coryphaena hippurus Species 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000223935 Cryptosporidium Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 108010041986 DNA Vaccines Proteins 0.000 description 1
- 229940021995 DNA vaccine Drugs 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 101150029707 ERBB2 gene Proteins 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 101710121417 Envelope glycoprotein Proteins 0.000 description 1
- 241001455610 Ephemerovirus Species 0.000 description 1
- 201000000297 Erysipelas Diseases 0.000 description 1
- 101000686777 Escherichia phage T7 T7 RNA polymerase Proteins 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 241000711475 Feline infectious peritonitis virus Species 0.000 description 1
- 241000714165 Feline leukemia virus Species 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000711950 Filoviridae Species 0.000 description 1
- 241000701047 Gallid alphaherpesvirus 2 Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 241000224466 Giardia Species 0.000 description 1
- 241000606807 Glaesserella parasuis Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 108060003393 Granulin Proteins 0.000 description 1
- 241000606768 Haemophilus influenzae Species 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- 208000037319 Hepatitis infectious Diseases 0.000 description 1
- 241000709721 Hepatovirus A Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- IDQNVIWPPWAFSY-AVGNSLFASA-N His-His-Gln Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCC(N)=O)C(O)=O IDQNVIWPPWAFSY-AVGNSLFASA-N 0.000 description 1
- 241000228402 Histoplasma Species 0.000 description 1
- 101000623901 Homo sapiens Mucin-16 Proteins 0.000 description 1
- 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 241000701806 Human papillomavirus Species 0.000 description 1
- 101100321817 Human parvovirus B19 (strain HV) 7.5K gene Proteins 0.000 description 1
- 241000726041 Human respirovirus 1 Species 0.000 description 1
- 241001559187 Human rubulavirus 2 Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000222732 Leishmania major Species 0.000 description 1
- 241000589929 Leptospira interrogans Species 0.000 description 1
- LJADEBULDNKJNK-IHRRRGAJSA-N Lys-Leu-Val Chemical compound CC(C)C[C@H](NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O LJADEBULDNKJNK-IHRRRGAJSA-N 0.000 description 1
- 241000711828 Lyssavirus Species 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001115401 Marburgvirus Species 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241000893980 Microsporum canis Species 0.000 description 1
- 241000711513 Mononegavirales Species 0.000 description 1
- 241000588655 Moraxella catarrhalis Species 0.000 description 1
- 241000712045 Morbillivirus Species 0.000 description 1
- 102100034256 Mucin-1 Human genes 0.000 description 1
- 108010008707 Mucin-1 Proteins 0.000 description 1
- 241000711408 Murine respirovirus Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 241000204022 Mycoplasma gallisepticum Species 0.000 description 1
- 108700015872 N-acetyl-nor-muramyl-L-alanyl-D-isoglutamine Proteins 0.000 description 1
- 108700020354 N-acetylmuramyl-threonyl-isoglutamine Proteins 0.000 description 1
- XMBSYZWANAQXEV-UHFFFAOYSA-N N-alpha-L-glutamyl-L-phenylalanine Natural products OC(=O)CCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XMBSYZWANAQXEV-UHFFFAOYSA-N 0.000 description 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 1
- 241000604972 Neorickettsia risticii Species 0.000 description 1
- 206010060860 Neurological symptom Diseases 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 208000010191 Osteitis Deformans Diseases 0.000 description 1
- 108700005081 Overlapping Genes Proteins 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 208000027868 Paget disease Diseases 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 241000282376 Panthera tigris Species 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 241000606856 Pasteurella multocida Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- MQWISMJKHOUEMW-ULQDDVLXSA-N Phe-Arg-His Chemical compound C([C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CC=1NC=NC=1)C(O)=O)C1=CC=CC=C1 MQWISMJKHOUEMW-ULQDDVLXSA-N 0.000 description 1
- 241000711899 Phocine morbillivirus Species 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- 241000233872 Pneumocystis carinii Species 0.000 description 1
- 241000711904 Pneumoviridae Species 0.000 description 1
- 241000711902 Pneumovirus Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 102000007066 Prostate-Specific Antigen Human genes 0.000 description 1
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- 241000588767 Proteus vulgaris Species 0.000 description 1
- 102000009572 RNA Polymerase II Human genes 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241001113283 Respirovirus Species 0.000 description 1
- 241000710799 Rubella virus Species 0.000 description 1
- 241001533467 Rubulavirus Species 0.000 description 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 101710183568 Serine/threonine-protein kinase PknK Proteins 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000193985 Streptococcus agalactiae Species 0.000 description 1
- 206010043376 Tetanus Diseases 0.000 description 1
- 108010055044 Tetanus Toxin Proteins 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 241000223997 Toxoplasma gondii Species 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 241001467018 Typhis Species 0.000 description 1
- UNUZEBFXGWVAOP-DZKIICNBSA-N Tyr-Glu-Val Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O UNUZEBFXGWVAOP-DZKIICNBSA-N 0.000 description 1
- LAYSXAOGWHKNED-XPUUQOCRSA-N Val-Gly-Ser Chemical compound CC(C)[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O LAYSXAOGWHKNED-XPUUQOCRSA-N 0.000 description 1
- 241000711970 Vesiculovirus Species 0.000 description 1
- 241000607626 Vibrio cholerae Species 0.000 description 1
- 239000003070 absorption delaying agent Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine group Chemical group [C@@H]1([C@H](O)[C@H](O)[C@@H](CO)O1)N1C=NC=2C(N)=NC=NC12 OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 108010008685 alanyl-glutamyl-aspartic acid Proteins 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- 230000003942 amyloidogenic effect Effects 0.000 description 1
- 206010002022 amyloidosis Diseases 0.000 description 1
- 244000037640 animal pathogen Species 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 239000003429 antifungal agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000010310 bacterial transformation Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 208000002352 blister Diseases 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 208000019065 cervical carcinoma Diseases 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000002962 chemical mutagen Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229940038705 chlamydia trachomatis Drugs 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 239000002612 dispersion medium Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 201000002491 encephalomyelitis Diseases 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 208000018685 gastrointestinal system disease Diseases 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 238000001476 gene delivery Methods 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
- 108010087823 glycyltyrosine Proteins 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000005252 hepatitis A Diseases 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 229940124452 immunizing agent Drugs 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229940117681 interleukin-12 Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000007951 isotonicity adjuster Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 238000003670 luciferase enzyme activity assay Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 230000017156 mRNA modification Effects 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 208000027202 mammary Paget disease Diseases 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- JMUHBNWAORSSBD-WKYWBUFDSA-N mifamurtide Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCCCCCCCCCC)COP(O)(=O)OCCNC(=O)[C@H](C)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@H](C)NC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1NC(C)=O JMUHBNWAORSSBD-WKYWBUFDSA-N 0.000 description 1
- 229960005225 mifamurtide Drugs 0.000 description 1
- 108700007621 mifamurtide Proteins 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- QUSNBJAOOMFDIB-UHFFFAOYSA-N monoethyl amine Natural products CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 1
- 229940035032 monophosphoryl lipid a Drugs 0.000 description 1
- 201000006417 multiple sclerosis Diseases 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 231100000189 neurotoxic Toxicity 0.000 description 1
- 230000002887 neurotoxic effect Effects 0.000 description 1
- 230000005156 neurotropism Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229940051027 pasteurella multocida Drugs 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 229940007042 proteus vulgaris Drugs 0.000 description 1
- 208000009305 pseudorabies Diseases 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 208000003265 stomatitis Diseases 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 229940118376 tetanus toxin Drugs 0.000 description 1
- 229960000814 tetanus toxoid Drugs 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
- C12N2760/18422—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
- C12N2760/18451—Methods of production or purification of viral material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Communicable Diseases (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21369800P | 2000-06-23 | 2000-06-23 | |
| US60/213,698 | 2000-06-23 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020087032094A Division KR20090006239A (ko) | 2000-06-23 | 2001-06-22 | ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20030013462A true KR20030013462A (ko) | 2003-02-14 |
Family
ID=22796152
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020027017579A Ceased KR20030013462A (ko) | 2000-06-23 | 2001-06-22 | ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 |
| KR1020087032094A Ceased KR20090006239A (ko) | 2000-06-23 | 2001-06-22 | ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020087032094A Ceased KR20090006239A (ko) | 2000-06-23 | 2001-06-22 | ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20050089985A1 (enExample) |
| EP (1) | EP1303613A2 (enExample) |
| JP (1) | JP2004501646A (enExample) |
| KR (2) | KR20030013462A (enExample) |
| CN (1) | CN1455816A (enExample) |
| AU (1) | AU2001271423A1 (enExample) |
| BR (1) | BR0112384A (enExample) |
| CA (1) | CA2412621A1 (enExample) |
| IL (1) | IL152930A0 (enExample) |
| MX (1) | MXPA02012404A (enExample) |
| WO (1) | WO2002000883A2 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003289858A (ja) * | 2002-04-05 | 2003-10-14 | Nippon Biologicals Inc | イヌジステンパー及びリーシュマニア感染症に対する組換え体イヌジステンパーウイルスワクチン |
| WO2004113517A2 (en) * | 2003-06-09 | 2004-12-29 | Wyeth | IMPROVED METHOD FOR THE RECOVERY OF NON-SEGMENTED, NEGATIVE-STRANDED RNA VIRUSES FROM cDNA |
| CN101228272A (zh) * | 2005-04-20 | 2008-07-23 | 生物载体株式会社 | 用于治疗阿尔茨海默病的具有较高安全性的经鼻腔内给药基因疫苗 |
| US7682619B2 (en) | 2006-04-06 | 2010-03-23 | Cornell Research Foundation, Inc. | Canine influenza virus |
| US8227583B2 (en) | 2007-06-14 | 2012-07-24 | The Board Of Regents For Oklahoma State University | Vaccines containing canine parvovirus genetic variants |
| JP5893829B2 (ja) | 2007-06-14 | 2016-03-23 | ザ・ボード・オブ・リージェンツ・フォー・オクラホマ・ステート・ユニバーシティ | イヌパルボウイルス遺伝子変異株を含むワクチン |
| EP2363472B1 (en) * | 2008-10-31 | 2014-12-10 | Dnavec Corporation | Method for enhancing expression of recombinant protein |
| EP2391713A1 (en) | 2009-01-30 | 2011-12-07 | The Board Of Regents For Oklahoma State University | Immunogenic compositions, vaccines and diagnostics based on canine distemper viruses circulating in north american dogs |
| EP2491117B2 (en) † | 2009-10-20 | 2017-06-28 | Novartis AG | Improved reverse genetics methods for virus rescue |
| CN102329809B (zh) * | 2010-07-12 | 2014-02-26 | 中国农业科学院哈尔滨兽医研究所 | 犬瘟热病毒cdv/r-20/8疫苗株的反向遗传操作系统及其应用 |
| CN102586485B (zh) * | 2012-03-12 | 2013-09-04 | 中国农业科学院哈尔滨兽医研究所 | 用于犬瘟热病毒野毒株与疫苗株鉴别诊断rt-lamp检测引物及其应用 |
| KR101595445B1 (ko) | 2013-12-31 | 2016-02-19 | 대한민국 | 수정진동자 바이오센서를 구비하는 개 디스템퍼 바이러스 검출 장치 및 이를 이용한 검출 방법 |
| EP3334828B1 (en) * | 2015-08-10 | 2020-10-28 | CureVac Real Estate GmbH | Method of increasing the replication of a circular dna molecule |
| KR101940674B1 (ko) * | 2017-05-19 | 2019-01-21 | 전북대학교 산학협력단 | 신경계 감염병 원인체를 검출하기 위한 조성물 |
| CN111093698B (zh) * | 2017-09-23 | 2024-04-05 | 勃林格殷格翰动物保健有限公司 | 副粘病毒科表达系统 |
| CN112867506B (zh) * | 2018-09-20 | 2025-08-05 | 勃林格殷格翰动物保健有限公司 | 抗猪流行性腹泻的鼻内载体疫苗 |
| CN109750006A (zh) * | 2019-01-14 | 2019-05-14 | 青岛农业大学 | 一种犬瘟热病毒复制缺陷毒株及其构建方法 |
| CN111733170A (zh) * | 2020-07-01 | 2020-10-02 | 青岛农业大学 | 一种表达荧光素酶的重组犬麻疹病毒 |
| CN113462656B (zh) * | 2021-03-24 | 2022-09-30 | 兰州生物制品研究所有限责任公司 | 一种人三型副流感病毒冷适应温度敏感株及其应用 |
| CN113416750A (zh) * | 2021-07-05 | 2021-09-21 | 青岛农业大学 | 一种表达犬细小病毒2a型VP2的重组犬瘟热病毒 |
| CN117653723B (zh) * | 2023-12-01 | 2024-07-26 | 中山迈托姆生物技术有限公司 | 一种重组犬瘟热病毒疫苗的纯化方法及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3155589A (en) * | 1958-04-14 | 1964-11-03 | Upjohn Co | Parenteral extravascular injectable vaccines for simultaneous immunization of canidae against rabies, canine distemper, and infectious canine hepatitis |
| US3354038A (en) * | 1964-12-02 | 1967-11-21 | Affiliated Lab Corp | Serial passaging tissue cultured canine distemper virus to form attenuated vaccine short of virus-antigenicity decreasing passages |
| US5166057A (en) * | 1989-08-28 | 1992-11-24 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand rna virus expression-systems |
| JP2521226B2 (ja) * | 1993-01-28 | 1996-08-07 | 株式会社微生物化学研究所 | 犬用多種混合ワクチン |
| ES2210273T5 (es) * | 1994-07-18 | 2010-03-29 | Conzelmann, Karl-Klaus, Prof. Dr. | Virus con arn de cadena negativa no segmentado recombinante infeccioso. |
-
2001
- 2001-06-22 KR KR1020027017579A patent/KR20030013462A/ko not_active Ceased
- 2001-06-22 KR KR1020087032094A patent/KR20090006239A/ko not_active Ceased
- 2001-06-22 IL IL15293001A patent/IL152930A0/xx unknown
- 2001-06-22 BR BR0112384-0A patent/BR0112384A/pt not_active Application Discontinuation
- 2001-06-22 EP EP01950430A patent/EP1303613A2/en not_active Ceased
- 2001-06-22 WO PCT/US2001/020157 patent/WO2002000883A2/en not_active Ceased
- 2001-06-22 CN CN01811601A patent/CN1455816A/zh active Pending
- 2001-06-22 AU AU2001271423A patent/AU2001271423A1/en not_active Abandoned
- 2001-06-22 CA CA002412621A patent/CA2412621A1/en not_active Abandoned
- 2001-06-22 MX MXPA02012404A patent/MXPA02012404A/es not_active Application Discontinuation
- 2001-06-22 US US10/312,052 patent/US20050089985A1/en not_active Abandoned
- 2001-06-22 JP JP2002506198A patent/JP2004501646A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL152930A0 (en) | 2003-06-24 |
| US20050089985A1 (en) | 2005-04-28 |
| CA2412621A1 (en) | 2002-01-03 |
| CN1455816A (zh) | 2003-11-12 |
| EP1303613A2 (en) | 2003-04-23 |
| KR20090006239A (ko) | 2009-01-14 |
| JP2004501646A (ja) | 2004-01-22 |
| BR0112384A (pt) | 2003-09-23 |
| MXPA02012404A (es) | 2003-04-25 |
| WO2002000883A3 (en) | 2003-02-06 |
| WO2002000883A2 (en) | 2002-01-03 |
| AU2001271423A1 (en) | 2002-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20030013462A (ko) | ｃＤＮＡ로 부터 개 홍역 바이러스의 구출 | |
| KR100992235B1 (ko) | 재조합 뉴캐슬 질병 바이러스 ｒｎａ 발현계 및 백신 | |
| EP0994949B1 (en) | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences | |
| KR101745029B1 (ko) | 재조합 조류 파라믹소바이러스 백신 및 이의 제조 및 사용 방법 | |
| JPH10506782A (ja) | 組換え型シチメンチョウヘルペスウイルス、及びその使用 | |
| EP1196197B1 (en) | Recombinant parainfluenza virus vaccines attenuated by deletion or ablation of a non-essential gene | |
| JP2001511142A (ja) | ベクターとして鳥類の感染性喉頭気管炎ウイルスを用いた鳥類用組換え生ワクチン | |
| KR20090007706A (ko) | 조류 인플루엔자 바이러스의 ｈ5 헤마글루티닌을 발현하는 재조합 뉴캐슬병 바이러스 | |
| KR100940776B1 (ko) | 약독화된 사람-소 키메라 파라인플루엔자바이러스(ｐｉｖ) 백신 | |
| US7208161B1 (en) | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences | |
| MXPA01010273A (es) | Novedosos herpesvirus recombinantes y mutantes. | |
| US6913751B2 (en) | Recombinant avian herpesvirus useful in vaccine production | |
| KR20020092898A (ko) | 씨디엔에이로부터 멈프스 바이러스의 구제 | |
| US20220125909A1 (en) | Chimeric rsv and hmpv f proteins, immunogenic compositions, and methods of use | |
| JP2005040129A (ja) | 麻疹ウイルス組換え体ポックスウイルスワクチン | |
| US20100008948A1 (en) | Recombinant herpesvirus useful in vaccine production | |
| AU775514B2 (en) | A recombinant Newcastle Disease virus as an embryo vaccine | |
| AU2007203671B2 (en) | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences | |
| EP1074614B1 (en) | A recombinant Newcastle disease virus for in ovo vaccination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20021223 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| AMND | Amendment | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20060621 Comment text: Request for Examination of Application |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20070625 Patent event code: PE09021S01D |
|
| AMND | Amendment | ||
| E601 | Decision to refuse application | ||
| PE0601 | Decision on rejection of patent |
Patent event date: 20080930 Comment text: Decision to Refuse Application Patent event code: PE06012S01D Patent event date: 20070625 Comment text: Notification of reason for refusal Patent event code: PE06011S01I |
|
| A107 | Divisional application of patent | ||
| AMND | Amendment | ||
| PA0104 | Divisional application for international application |
Comment text: Divisional Application for International Patent Patent event code: PA01041R01D Patent event date: 20081230 |
|
| PJ0201 | Trial against decision of rejection |
Patent event date: 20081230 Comment text: Request for Trial against Decision on Refusal Patent event code: PJ02012R01D Patent event date: 20080930 Comment text: Decision to Refuse Application Patent event code: PJ02011S01I Appeal kind category: Appeal against decision to decline refusal Decision date: 20101027 Appeal identifier: 2008101014096 Request date: 20081230 |
|
| PB0901 | Examination by re-examination before a trial |
Comment text: Amendment to Specification, etc. Patent event date: 20081230 Patent event code: PB09011R02I Comment text: Request for Trial against Decision on Refusal Patent event date: 20081230 Patent event code: PB09011R01I Comment text: Amendment to Specification, etc. Patent event date: 20080526 Patent event code: PB09011R02I Comment text: Amendment to Specification, etc. Patent event date: 20060621 Patent event code: PB09011R02I |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20090225 Patent event code: PE09021S01D |
|
| B601 | Maintenance of original decision after re-examination before a trial | ||
| PB0601 | Maintenance of original decision after re-examination before a trial | ||
| PJ1301 | Trial decision |
Patent event code: PJ13011S01D Patent event date: 20101028 Comment text: Trial Decision on Objection to Decision on Refusal Appeal kind category: Appeal against decision to decline refusal Request date: 20081230 Decision date: 20101027 Appeal identifier: 2008101014096 |
|
| PC1202 | Submission of document of withdrawal before decision of registration |
Comment text: [Withdrawal of Procedure relating to Patent, etc.] Withdrawal (Abandonment) Patent event code: PC12021R01D Patent event date: 20101228 |
|
| WITB | Written withdrawal of application |