KR20000023814A - 표적 특이적 Ｔｍ이 증가된 변형 올리고뉴클레오티드를 사용한 핵산 서열의 검출 및 증폭 방법 - Google Patents
표적 특이적 Ｔｍ이 증가된 변형 올리고뉴클레오티드를 사용한 핵산 서열의 검출 및 증폭 방법 Download PDFInfo
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- KR20000023814A KR20000023814A KR1019997000300A KR19997000300A KR20000023814A KR 20000023814 A KR20000023814 A KR 20000023814A KR 1019997000300 A KR1019997000300 A KR 1019997000300A KR 19997000300 A KR19997000300 A KR 19997000300A KR 20000023814 A KR20000023814 A KR 20000023814A
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Classifications
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
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- Immunology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
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| US2181896P | 1996-07-16 | 1996-07-16 | |
| US60/021,818 | 1996-07-16 |
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| KR20000023814A true KR20000023814A (ko) | 2000-04-25 |
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| KR1019997000300A Withdrawn KR20000023814A (ko) | 1996-07-16 | 1999-01-15 | 표적 특이적 Ｔｍ이 증가된 변형 올리고뉴클레오티드를 사용한 핵산 서열의 검출 및 증폭 방법 |
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|---|---|
| US (3) | US6130038A (enExample) |
| EP (1) | EP0912767B1 (enExample) |
| JP (2) | JP2000514307A (enExample) |
| KR (1) | KR20000023814A (enExample) |
| AT (1) | ATE339514T1 (enExample) |
| AU (1) | AU726821B2 (enExample) |
| CA (1) | CA2260749A1 (enExample) |
| DE (1) | DE69736667T2 (enExample) |
| DK (1) | DK0912767T3 (enExample) |
| ES (1) | ES2270467T3 (enExample) |
| WO (1) | WO1998002582A2 (enExample) |
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| US5714380A (en) | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| US7070925B1 (en) * | 1996-07-16 | 2006-07-04 | Gen-Probe Incorporated | Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe |
| DK0912767T3 (da) * | 1996-07-16 | 2006-10-30 | Gen Probe Inc | Fremgangsmåde til sporing og forstærkning af nukleinsyresekvenser ved anvendelse af modificerede oligonukleotider med foröget targetspecifikt TM |
| JP3981416B2 (ja) | 1997-04-10 | 2007-09-26 | ユニバーシティ メディカル センター ナイメーヘン | Ｐｃａ３タンパク質、ｐｃａ３遺伝子、及びこれらの用途 |
| JP4646404B2 (ja) | 1998-07-02 | 2011-03-09 | ジェン−プローブ・インコーポレーテッド | 分子トーチ（ｔｏｒｃｈ） |
| CA2336539A1 (en) | 1998-07-31 | 2000-02-10 | Gen-Probe Incorporated | Reversible inhibiting probes |
| US6087112A (en) | 1998-12-30 | 2000-07-11 | Oligos Etc. Inc. | Arrays with modified oligonucleotide and polynucleotide compositions |
| AU5269600A (en) * | 1999-05-13 | 2000-12-05 | Oligos Etc. Inc. | Arrays with modified oligonucleotide and polynucleotide compositions |
| AU7636400A (en) | 1999-09-29 | 2001-04-30 | Diagnocure Inc. | Pca3 messenger rna species in benign and malignant prostate tissues |
| US6844151B1 (en) | 1999-09-29 | 2005-01-18 | Oligos Etc. Inc. | Methods for production of arrays with modified oligonucleotide and polynucleotide compositions |
| WO2001023620A2 (en) * | 1999-09-29 | 2001-04-05 | Oligos Etc. Inc. | Arrays with modified oligonucleotide and polynucleotide compositions |
| GB2355791B (en) * | 1999-10-28 | 2004-10-20 | Molecular Light Tech Res Ltd | Detection of mRNA expression using chemiluminescent labelled probes |
| US6924108B2 (en) * | 1999-12-21 | 2005-08-02 | Ingeneus Corporation | Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues |
| US7833715B1 (en) * | 2000-01-11 | 2010-11-16 | Nanogen Recognomics Gmbh | Biomolecules having multiple attachment moieties for binding to a substrate surface |
| EP1294751B1 (en) | 2000-03-31 | 2014-03-05 | Merial Limited | Dermacentor variabilis gaba-gated chloride channels |
| JP4950414B2 (ja) * | 2000-08-03 | 2012-06-13 | 独立行政法人産業技術総合研究所 | 核酸の測定方法、それに用いる核酸プローブ及びその方法によって得られるデータを解析する方法 |
| EP2020450B9 (en) | 2000-09-01 | 2011-09-14 | Gen-Probe Incorporated | Amplification of HIV-1 sequences for detection of sequences associated with drug-resistance mutations |
| US7081527B2 (en) * | 2000-09-12 | 2006-07-25 | Gen-Probe Incorporated | Compositions, methods and kits for determining the presence of Cryptosporidium parvum organisms in a test sample |
| ES2254306T3 (es) * | 2000-10-25 | 2006-06-16 | F. Hoffmann-La Roche Ag | Amplificacion usando cebadores modificados. |
| EP1251183A3 (en) * | 2001-04-18 | 2003-12-10 | Exiqon A/S | Improved helper probes for detection of a target sequence by a capture oligonucleotide |
| WO2002097394A2 (en) | 2001-05-31 | 2002-12-05 | Merck & Co., Inc. | Rhesus monkey, dog and ferret melanin-concentrating hormone type 2 receptor |
| AU2002316241A1 (en) * | 2001-06-12 | 2002-12-23 | The Regents Of The University Of California | Wit 3.0, a novel gene to control soft tissue wound healing |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US20040009506A1 (en) * | 2002-03-29 | 2004-01-15 | Genentech, Inc. | Methods and compositions for detection and quantitation of nucleic acid analytes |
| US7211382B2 (en) * | 2002-04-09 | 2007-05-01 | Orchid Cellmark Inc. | Primer extension using modified nucleotides |
| US7199107B2 (en) * | 2002-05-23 | 2007-04-03 | Isis Pharmaceuticals, Inc. | Antisense modulation of kinesin-like 1 expression |
| US20030235827A1 (en) * | 2002-06-25 | 2003-12-25 | Orchid Biosciences, Inc. | Methods and compositions for monitoring primer extension and polymorphism detection reactions |
| CN100354298C (zh) * | 2002-06-28 | 2007-12-12 | 兰华生物科技公司 | 使用单核苷酸多态性组分析受损样品的方法和组合物 |
| US7192700B2 (en) * | 2002-12-20 | 2007-03-20 | Orchid Cellmark Inc. | Methods and compositions for conducting primer extension and polymorphism detection reactions |
| CA2497570A1 (en) * | 2002-08-02 | 2004-02-12 | Orchid Biosciences, Inc. | Methods and compositions for genotyping |
| US7319041B2 (en) * | 2002-09-27 | 2008-01-15 | Siemens Medical Solutions Diagnostic | Applications of acridinium compounds and derivatives in homogeneous assays |
| WO2004067726A2 (en) * | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Isothermal reactions for the amplification of oligonucleotides |
| ES2427853T3 (es) | 2003-02-07 | 2013-11-04 | Diagnocure Inc. | Procedimiento para detectar cáncer de próstata en una muestra |
| CA2525413C (en) * | 2003-05-19 | 2010-08-17 | Gen-Probe Incorporated | Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample |
| US20080176220A1 (en) * | 2004-02-26 | 2008-07-24 | Thomsen Bioscience | Method, Kit and System for Enhanced Nested Pcr |
| CA2572472C (en) | 2004-07-01 | 2013-08-13 | Gen-Probe Incorporated | Methods and compositions to detect nucleic acids in a biological sample |
| DE602005026730D1 (de) | 2004-08-27 | 2011-04-14 | Gen Probe Inc | Einfach-Primernukleinsäuren-Erweiterungsverfahren |
| US7713697B2 (en) * | 2004-08-27 | 2010-05-11 | Gen-Probe Incorporated | Methods and kits for amplifying DNA |
| CA2491067A1 (en) | 2004-12-24 | 2006-06-24 | Stichting Katholieke Universiteit | Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer |
| EP1877581B1 (en) | 2005-05-06 | 2012-03-28 | Gen-Probe Incorporated | Methods and products for nucleic acid target capture |
| WO2006133385A2 (en) | 2005-06-06 | 2006-12-14 | Gen-Probe Incorporated | Compositions, methods and kits for determining the presence of chlamydophila pneumoniae in a test sample |
| EP2345740B1 (en) | 2006-06-06 | 2015-09-30 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| EP3159417B1 (en) | 2006-08-01 | 2021-10-06 | Gen-Probe Incorporated | Methods of nonspecific target capture of nucleic acids |
| EP2057465A4 (en) | 2006-08-09 | 2010-04-21 | Homestead Clinical Corp | ORGANIC PROTEINS AND METHOD OF USE THEREOF |
| US8183359B2 (en) * | 2007-03-01 | 2012-05-22 | Gen-Probe Incorporated | Kits for amplifying DNA |
| EP1978111B1 (en) | 2007-04-02 | 2013-03-27 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa |
| US9689031B2 (en) * | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| US8911946B2 (en) | 2007-12-21 | 2014-12-16 | Gen-Probe Incorporated | Detection of antibiotic-resistant microorganisms |
| US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
| US20090325169A1 (en) | 2008-04-30 | 2009-12-31 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US9434988B2 (en) * | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2009140374A2 (en) * | 2008-05-13 | 2009-11-19 | Gen-Probe Incorporated | Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences |
| AU2009262870B2 (en) | 2008-05-30 | 2014-03-20 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Salmonella |
| JP2012509675A (ja) | 2008-11-25 | 2012-04-26 | ジェン−プロウブ インコーポレイテッド | 低分子ｒｎａを検出するための組成物および方法、ならびにその使用 |
| US9394333B2 (en) | 2008-12-02 | 2016-07-19 | Wave Life Sciences Japan | Method for the synthesis of phosphorus atom modified nucleic acids |
| EP2910647A1 (en) | 2008-12-30 | 2015-08-26 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of listeria |
| WO2010126913A1 (en) | 2009-04-27 | 2010-11-04 | Gen-Probe Incorporated | Methods and kits for use in the selective amplification of target sequences |
| EP2451461A4 (en) | 2009-07-06 | 2013-05-29 | Ontorii Inc | NOVEL NUCLEIC ACID PRODRUGS AND METHOD OF USE THEREOF |
| US8999675B2 (en) | 2009-08-31 | 2015-04-07 | Gen-Probe Incorporated | Dengue virus assay |
| AU2011207406B2 (en) * | 2010-01-22 | 2015-01-15 | Gen-Probe Incorporated | Probes for detecting the presence of Trichomonas vaginalis in a sample |
| CN102985566B (zh) | 2010-06-30 | 2015-06-17 | 简.探针公司 | 使用分析物和过程控制信号的单次读取确定而鉴定含分析物的样品的方法和设备 |
| BR112013004044A2 (pt) | 2010-08-13 | 2016-07-05 | Envirologix Inc | composições e métodos para quantificação de uma sequência de ácido nucleico em uma amostra. |
| DK2620428T3 (da) | 2010-09-24 | 2019-07-01 | Wave Life Sciences Ltd | Asymmetrisk hjælpegruppe |
| BR112014001244A2 (pt) | 2011-07-19 | 2017-02-21 | Wave Life Sciences Pte Ltd | métodos para a síntese de ácidos nucléicos funcionalizados |
| US9175337B2 (en) | 2011-09-21 | 2015-11-03 | Gen-Probe Incorporated | Methods for amplifying nucleic acid using tag-mediated displacement |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| KR102159818B1 (ko) * | 2012-04-09 | 2020-09-25 | 엔바이로로직스 인크. | 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법 |
| EP2839024B1 (en) | 2012-04-16 | 2017-09-06 | Life Technologies Corporation | Methods for the preparation of rna libraries |
| MX356830B (es) | 2012-07-13 | 2018-06-15 | Shin Nippon Biomedical Laboratories Ltd | Adyuvante de acido nucleico quiral. |
| CN104684893B (zh) | 2012-07-13 | 2016-10-26 | 日本波涛生命科学公司 | 不对称辅助基团 |
| SG10201912895PA (en) | 2012-07-13 | 2020-02-27 | Wave Life Sciences Ltd | Chiral control |
| AU2013202793B2 (en) | 2012-07-31 | 2014-09-18 | Gen-Probe Incorporated | System, method and apparatus for automated incubation |
| WO2014140165A1 (en) | 2013-03-14 | 2014-09-18 | Dsm Ip Assets B.V. | Cell wall deconstruction enzymes of paecilomyces byssochlamydoides and uses thereof |
| WO2014140167A1 (en) | 2013-03-14 | 2014-09-18 | Dsm Ip Assets B.V. | Cell wall deconstruction enzymes of malbranchea cinnamomea and uses thereof |
| WO2014152054A1 (en) | 2013-03-15 | 2014-09-25 | Bio-Rad Laboratories, Inc. | Digital assays for mutation detection |
| US10752940B2 (en) | 2013-11-08 | 2020-08-25 | Ionis Pharmaceuticals, Inc. | Compounds and methods for detecting oligonucleotides |
| US10149905B2 (en) | 2014-01-15 | 2018-12-11 | Shin Nippon Biomedical Laboratories, Ltd. | Chiral nucleic acid adjuvant having antitumor effect and antitumor agent |
| EP3095460A4 (en) | 2014-01-15 | 2017-08-23 | Shin Nippon Biomedical Laboratories, Ltd. | Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent |
| JPWO2015108047A1 (ja) | 2014-01-15 | 2017-03-23 | 株式会社新日本科学 | 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤 |
| RU2016133035A (ru) | 2014-01-16 | 2018-02-21 | Уэйв Лайф Сайенсес Лтд. | Хиральный дизайн |
| US11505836B2 (en) | 2014-04-22 | 2022-11-22 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting DNA amplification |
| AU2015336086C1 (en) | 2014-10-20 | 2021-07-29 | Envirologix Inc. | Compositions and methods for detecting an RNA virus |
| KR102657306B1 (ko) | 2014-12-08 | 2024-04-12 | 버그 엘엘씨 | 전립선암의 진단 및 치료에서 필라민을 포함하는 마커의 용도 |
| CN114250274A (zh) | 2015-04-24 | 2022-03-29 | 阿提拉生物系统公司 | 利用有限核苷酸组成的引物扩增 |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| US12460254B2 (en) | 2018-08-08 | 2025-11-04 | Gen-Probe Incorporated | Compositions, methods and kits for detecting Mycoplasma genitalium |
| EP3914732A1 (en) | 2019-01-25 | 2021-12-01 | Gen-Probe Incorporated | Detection of drug-resistant mycoplasma genitalium |
| KR20220015394A (ko) | 2019-04-30 | 2022-02-08 | 라리마 테라퓨틱스, 인코포레이티드 | 프라탁신 대체 요법의 유효성 결정을 위한 프라탁신 민감성 마커 |
| US20220307093A1 (en) | 2019-07-03 | 2022-09-29 | Gen-Probe Incorporated | Oligonucleotides for use in determining the presence of trichomonas vaginalis in a sample |
| EP4018004B1 (en) | 2019-08-23 | 2025-06-18 | Gen-Probe Incorporated | Compositions, methods and kits for detecting treponema pallidum |
| US20230243001A1 (en) | 2020-07-17 | 2023-08-03 | Gen-Probe Incorporated | Detection of Macrolide-Resistant Mycoplasma Genitalium |
| WO2022047359A1 (en) | 2020-08-31 | 2022-03-03 | Berg Llc | Protein biomarkers for pancreatic cancer |
| EP4320443A1 (en) | 2021-04-06 | 2024-02-14 | BPGbio, Inc. | Protein markers for the prognosis of breast cancer progression |
| US20240402177A1 (en) | 2021-04-06 | 2024-12-05 | Bpgbio, Inc. | Protein markers for estrogen receptor (er)-positive luminal a (la)-like and luminal b1 (lb1)-like breast cancer |
| US20230059578A1 (en) | 2021-04-06 | 2023-02-23 | Berg Llc | Protein markers for estrogen receptor (er)-positive-like and estrogen receptor (er)-negative-like breast cancer |
| US20230357851A1 (en) | 2022-04-06 | 2023-11-09 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for monitoring frataxin-replacement therapy |
| WO2023240201A1 (en) | 2022-06-08 | 2023-12-14 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for monitoring progression and treatment of leigh syndrome |
| WO2024220373A1 (en) | 2023-04-15 | 2024-10-24 | Accent Therapeutics, Inc. | Assays for monitoring inhibition of rna helicase dhx9 |
| WO2025155991A2 (en) | 2024-01-19 | 2025-07-24 | Bpgbio, Inc. | Predictive biomarkers for response to coenzyme q10 treatment in pancreatic cancer |
Family Cites Families (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5087558A (en) | 1981-09-25 | 1992-02-11 | Webster John A Jr | Method for identifying and characterizing organisms |
| US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| US4820630A (en) * | 1984-11-23 | 1989-04-11 | Digene Diagnostics, Incorporated | Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels |
| JPS61243182A (ja) * | 1985-04-19 | 1986-10-29 | Nitsukan Kogyo Kk | 金属メツキされたチタン酸カリウム繊維およびその製造方法 |
| US4822733A (en) * | 1985-05-28 | 1989-04-18 | Amoco Corporation | Lifetime-resolved assay procedures |
| US4797355A (en) * | 1985-06-13 | 1989-01-10 | Amgen Inc. | Methods for attaching polynucleotides to supports |
| US4996143A (en) * | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
| DE3785591T2 (de) * | 1986-01-10 | 1993-09-02 | Amoco Corp | Kompetitiver homogener test. |
| US4925785A (en) * | 1986-03-07 | 1990-05-15 | Biotechnica Diagnostics, Inc. | Nucleic acid hybridization assays |
| US5091519A (en) * | 1986-05-01 | 1992-02-25 | Amoco Corporation | Nucleotide compositions with linking groups |
| IE72468B1 (en) | 1987-07-31 | 1997-04-09 | Univ Leland Stanford Junior | Selective amplification of target polynucleotide sequences |
| US5030557A (en) * | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| ES2093633T3 (es) * | 1989-01-19 | 1997-01-01 | Behringwerke Ag | Amplificacion de acidos nucleicos utilizando un unico cebador. |
| DE69030804T2 (de) * | 1989-04-05 | 1997-11-06 | Vysis Inc | Reagenzien zur verbesserung von hybridisierung |
| IE81145B1 (en) | 1989-04-20 | 2000-05-03 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
| WO1990014442A1 (en) * | 1989-05-24 | 1990-11-29 | Gene-Trak Systems | Nucleic acid probes for the detection of neisseria gonorrhoeae |
| US5849504A (en) | 1989-08-11 | 1998-12-15 | Amoco Corporation | Nucleic acid probes and methods for detecting Pneumocystis carinii |
| US5134066A (en) * | 1989-08-29 | 1992-07-28 | Monsanto Company | Improved probes using nucleosides containing 3-dezauracil analogs |
| CA2025178A1 (en) * | 1989-10-02 | 1991-04-03 | William G. Weisburg | Nucleic acid probes for the detection of lyme disease spirochetes |
| EP0503000A4 (en) * | 1989-12-01 | 1993-04-14 | The Board Of Trustees Of The Leland Stanford Junior University | Promotion of high specificity molecular assembly |
| US5914396A (en) | 1990-01-11 | 1999-06-22 | Isis Pharmaceuticals, Inc. | 2'-O-modified nucleosides and phosphoramidites |
| US5536638A (en) | 1990-04-18 | 1996-07-16 | N.V. Innogenetics S.A. | Hybridization probes derived from the spacer region between the 16S and 23S rRNA genes for the detection of Neisseria gonorrhoeae |
| US5811533A (en) | 1990-06-11 | 1998-09-22 | Nexstar Pharmaceuticals, Inc. | High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF) |
| BR9106702A (pt) * | 1990-07-27 | 1993-06-08 | Isis Pharmaceuticals Inc | Analogo de oligonucleotideos e processos para modular a producao de uma proteina por um organismo e para tratar um organismo |
| US5386023A (en) * | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| BR9106026A (pt) * | 1990-11-29 | 1993-01-05 | Kumiai Chemical Industry Co | Derivados de benzohidroximoilazol,metodo para a sua producao,e inseticida incluindo os mesmos, |
| US5641625A (en) * | 1992-05-22 | 1997-06-24 | Isis Pharmaceuticals, Inc. | Cleaving double-stranded DNA with peptide nucleic acids |
| US5435248A (en) * | 1991-07-09 | 1995-07-25 | The Ensign-Bickford Company | Extended range digital delay detonator |
| CA2122365C (en) * | 1991-11-26 | 2010-05-11 | Brian Froehler | Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines |
| DK0618925T4 (da) * | 1991-12-24 | 2012-07-09 | Isis Pharmaceuticals Inc | Antisense-oligonukleotider |
| US5700922A (en) * | 1991-12-24 | 1997-12-23 | Isis Pharmaceuticals, Inc. | PNA-DNA-PNA chimeric macromolecules |
| US5652099A (en) * | 1992-02-12 | 1997-07-29 | Conrad; Michael J. | Probes comprising fluorescent nucleosides and uses thereof |
| EP0566751B1 (en) * | 1992-03-23 | 1996-01-10 | F. Hoffmann-La Roche Ag | DNA detection method |
| EP0651759B1 (en) * | 1992-07-23 | 2003-12-10 | Isis Pharmaceuticals, Inc. | Novel 2'-o-alkyl nucleosides and phosphoramidites processes for the preparation and uses thereof |
| US5445933A (en) * | 1992-09-15 | 1995-08-29 | Boehringer Mannheim Corporation | Strand displacement assay and complex useful therefor |
| EP0601889A2 (en) * | 1992-12-10 | 1994-06-15 | Maine Medical Center Research Institute | Nucleic acid probes |
| AU3729493A (en) * | 1993-02-19 | 1994-09-14 | Ciba-Geigy Ag | Cyclobutyl antisense oligonucleotides, methods of making and use thereof |
| US5514546A (en) * | 1993-09-01 | 1996-05-07 | Research Corporation Technologies, Inc. | Stem-loop oligonucleotides containing parallel and antiparallel binding domains |
| US6156501A (en) | 1993-10-26 | 2000-12-05 | Affymetrix, Inc. | Arrays of modified nucleic acid probes and methods of use |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5827648A (en) * | 1995-12-13 | 1998-10-27 | Chiron Corporation | Differential hybridization for relative quantification of variant populations |
| SE9400522D0 (sv) * | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US6169169B1 (en) * | 1994-05-19 | 2001-01-02 | Dako A/S | PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis |
| ZA956776B (en) * | 1994-08-25 | 1996-03-20 | Akzo Nobel Nv | A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay |
| US5656427A (en) * | 1994-08-29 | 1997-08-12 | Gen-Probe Incorporated | Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid |
| US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US6001564A (en) * | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US5514551A (en) * | 1994-10-14 | 1996-05-07 | Gen-Probe Incorporated | Compositions for the detection of Chlamydia trachomatis |
| JP4040087B2 (ja) * | 1995-05-05 | 2008-01-30 | アプレラ コーポレイション | Ｐｃｒ増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬 |
| AU726844B2 (en) | 1995-06-07 | 2000-11-23 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands that bind to and inhibit DNA polymerases |
| US5731148A (en) | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
| US5747252A (en) * | 1995-06-07 | 1998-05-05 | Gen-Probe Incorporated | Nucleic acid probes and amplification oligonucleotides for Neisseria species |
| JP3898228B2 (ja) * | 1996-04-12 | 2007-03-28 | ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク | 検出プローブ、キット及びアッセイ |
| US6218107B1 (en) | 1996-05-22 | 2001-04-17 | Gen-Probe Incorporated | Compositions and methods for detecting the presence of Mycobacterium kansassii |
| US5898031A (en) * | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US7070925B1 (en) * | 1996-07-16 | 2006-07-04 | Gen-Probe Incorporated | Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe |
| DK0912767T3 (da) * | 1996-07-16 | 2006-10-30 | Gen Probe Inc | Fremgangsmåde til sporing og forstærkning af nukleinsyresekvenser ved anvendelse af modificerede oligonukleotider med foröget targetspecifikt TM |
| US6747252B2 (en) * | 1996-11-15 | 2004-06-08 | Kenneth J. Herzog | Multiple head induction sealer apparatus and method |
| US5846726A (en) * | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US6210015B1 (en) * | 1999-10-04 | 2001-04-03 | Kenneth Alan Ross | Stand for cakes or pastry |
-
1997
- 1997-07-15 DK DK97934169T patent/DK0912767T3/da active
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- 1997-07-15 AU AU37288/97A patent/AU726821B2/en not_active Expired
- 1997-07-15 JP JP10506265A patent/JP2000514307A/ja not_active Ceased
- 1997-07-15 CA CA002260749A patent/CA2260749A1/en not_active Abandoned
- 1997-07-15 ES ES97934169T patent/ES2270467T3/es not_active Expired - Lifetime
- 1997-07-15 US US08/893,300 patent/US6130038A/en not_active Expired - Lifetime
- 1997-07-15 AT AT97934169T patent/ATE339514T1/de not_active IP Right Cessation
-
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- 1999-01-15 KR KR1019997000300A patent/KR20000023814A/ko not_active Withdrawn
-
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- 2000-03-10 US US09/523,237 patent/US6903206B1/en not_active Expired - Lifetime
-
2004
- 2004-10-26 US US10/973,162 patent/US20050106610A1/en not_active Abandoned
-
2008
- 2008-05-27 JP JP2008137832A patent/JP4527789B2/ja not_active Expired - Lifetime
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| US6130038A (en) | 2000-10-10 |
| DE69736667D1 (de) | 2006-10-26 |
| DE69736667T2 (de) | 2007-09-06 |
| DK0912767T3 (da) | 2006-10-30 |
| JP4527789B2 (ja) | 2010-08-18 |
| ATE339514T1 (de) | 2006-10-15 |
| EP0912767B1 (en) | 2006-09-13 |
| WO1998002582A3 (en) | 1998-07-02 |
| US20050106610A1 (en) | 2005-05-19 |
| AU726821B2 (en) | 2000-11-23 |
| US6903206B1 (en) | 2005-06-07 |
| CA2260749A1 (en) | 1998-01-22 |
| ES2270467T3 (es) | 2007-04-01 |
| JP2000514307A (ja) | 2000-10-31 |
| JP2008283973A (ja) | 2008-11-27 |
| AU3728897A (en) | 1998-02-09 |
| WO1998002582A2 (en) | 1998-01-22 |
| EP0912767A2 (en) | 1999-05-06 |
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