KR102527830B1 - 고효율 형광 유세포 분석기를 사용하는 세포 선별 - Google Patents

고효율 형광 유세포 분석기를 사용하는 세포 선별 Download PDF

Info

Publication number
KR102527830B1
KR102527830B1 KR1020217042286A KR20217042286A KR102527830B1 KR 102527830 B1 KR102527830 B1 KR 102527830B1 KR 1020217042286 A KR1020217042286 A KR 1020217042286A KR 20217042286 A KR20217042286 A KR 20217042286A KR 102527830 B1 KR102527830 B1 KR 102527830B1
Authority
KR
South Korea
Prior art keywords
cell
fluorescence
frequency
particle
radiation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020217042286A
Other languages
English (en)
Korean (ko)
Other versions
KR20220002706A (ko
Inventor
에릭 다이볼드
키건 오슬리
조나단 린
Original Assignee
벡톤 디킨슨 앤드 컴퍼니
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 벡톤 디킨슨 앤드 컴퍼니 filed Critical 벡톤 디킨슨 앤드 컴퍼니
Priority to KR1020227045037A priority Critical patent/KR102641469B1/ko
Publication of KR20220002706A publication Critical patent/KR20220002706A/ko
Application granted granted Critical
Publication of KR102527830B1 publication Critical patent/KR102527830B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6402Atomic fluorescence; Laser induced fluorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/149Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • G01N2015/1447Spatial selection
    • G01N2015/149
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020217042286A 2016-03-17 2017-03-17 고효율 형광 유세포 분석기를 사용하는 세포 선별 Active KR102527830B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020227045037A KR102641469B1 (ko) 2016-03-17 2017-03-17 고효율 형광 유세포 분석기를 사용하는 세포 선별

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662309806P 2016-03-17 2016-03-17
US62/309,806 2016-03-17
KR1020187030001A KR20180129832A (ko) 2016-03-17 2017-03-17 고효율 형광 유세포분석기를 사용하는 세포 선별
PCT/US2017/022936 WO2017161247A1 (en) 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187030001A Division KR20180129832A (ko) 2016-03-17 2017-03-17 고효율 형광 유세포분석기를 사용하는 세포 선별

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020227045037A Division KR102641469B1 (ko) 2016-03-17 2017-03-17 고효율 형광 유세포 분석기를 사용하는 세포 선별

Publications (2)

Publication Number Publication Date
KR20220002706A KR20220002706A (ko) 2022-01-06
KR102527830B1 true KR102527830B1 (ko) 2023-05-02

Family

ID=58428427

Family Applications (3)

Application Number Title Priority Date Filing Date
KR1020217042286A Active KR102527830B1 (ko) 2016-03-17 2017-03-17 고효율 형광 유세포 분석기를 사용하는 세포 선별
KR1020227045037A Active KR102641469B1 (ko) 2016-03-17 2017-03-17 고효율 형광 유세포 분석기를 사용하는 세포 선별
KR1020187030001A Ceased KR20180129832A (ko) 2016-03-17 2017-03-17 고효율 형광 유세포분석기를 사용하는 세포 선별

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020227045037A Active KR102641469B1 (ko) 2016-03-17 2017-03-17 고효율 형광 유세포 분석기를 사용하는 세포 선별
KR1020187030001A Ceased KR20180129832A (ko) 2016-03-17 2017-03-17 고효율 형광 유세포분석기를 사용하는 세포 선별

Country Status (10)

Country Link
US (7) US10324019B2 (enExample)
EP (2) EP3430376B8 (enExample)
JP (3) JP7130562B2 (enExample)
KR (3) KR102527830B1 (enExample)
CN (1) CN109564151B (enExample)
AU (1) AU2017234815B2 (enExample)
BR (1) BR112018068866B1 (enExample)
CA (1) CA3018065A1 (enExample)
WO (1) WO2017161247A1 (enExample)
ZA (1) ZA201806386B (enExample)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP2943778B1 (en) 2013-01-09 2020-10-28 The Regents of The University of California Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
ES2959506T3 (es) 2014-03-18 2024-02-26 Univ California Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método
CN105987870A (zh) * 2015-02-10 2016-10-05 博奥生物集团有限公司 一种流式细胞分选系统及其聚焦检测方法及其流体芯片
WO2017046806A1 (en) * 2015-09-17 2017-03-23 Technion Research & Development Foundation Limited Reflectance confocal microscopy of blood cells
KR102600559B1 (ko) 2015-10-13 2023-11-10 벡톤 디킨슨 앤드 컴퍼니 다중모드 형광 이미징 유동 세포 계측 시스템
CN109564151B (zh) 2016-03-17 2023-01-24 贝克顿·迪金森公司 使用高通量荧光流式细胞仪进行细胞分选
WO2017197271A1 (en) 2016-05-12 2017-11-16 Bd Biosciences Fluorescence imaging flow cytometry with enhanced image resolution
US10006852B2 (en) 2016-09-13 2018-06-26 Becton, Dickinson And Company Flow cytometer with optical equalization
US11579073B2 (en) 2017-02-07 2023-02-14 Nodexus Inc. Microfluidic system with combined electrical and optical detection for high accuracy particle sorting and methods thereof
US20180292662A1 (en) * 2017-04-05 2018-10-11 Osela Inc. Rectangular beam shaper having monolithic body of refractive material
US11169092B2 (en) * 2017-07-11 2021-11-09 Hamamatsu Photonics K.K. Sample observation device and sample observation method
EP3655753A1 (en) 2017-07-18 2020-05-27 Becton, Dickinson and Company Dynamic interactive display of multi-parameter quantitative biological data
US10593082B2 (en) 2017-07-18 2020-03-17 Becton, Dickinson And Company Dynamic display of multi-parameter quantitative biological data
WO2019018129A1 (en) 2017-07-18 2019-01-24 Becton, Dickinson And Company DYNAMIC INTERACTIVE DISPLAY OF QUANTITATIVE BIOLOGICAL DATA WITH MULTIPLE PARAMETERS
US11099129B2 (en) 2017-09-14 2021-08-24 Brown University Spatially resolved standoff trace chemical sensing using backwards transient absorption spectroscopy
US10466173B2 (en) * 2017-10-06 2019-11-05 Wyatt Technology Corporation Optical flow cell assembly incorporating a replaceable transparent flow cell
US10267723B1 (en) 2018-01-23 2019-04-23 Cbrn International, Ltd. Bioaerosol particle detector
WO2019161406A1 (en) * 2018-02-16 2019-08-22 The Regents Of The University Of California Systems, devices and methods for three-dimensional imaging of moving particles
EP3844299A1 (en) * 2018-08-28 2021-07-07 Becton Dickinson and Company Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
JP7489968B2 (ja) * 2018-09-14 2024-05-24 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 細胞選別装置及び方法
WO2020106036A1 (en) * 2018-11-19 2020-05-28 Samsung Electronics Co., Ltd. Multimodal dust sensor
CN113614511A (zh) 2019-01-11 2021-11-05 贝克顿·迪金森公司 优化的分选门
EP3942279A4 (en) 2019-03-21 2022-12-28 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS AND METHODS OF USE THEREOF
WO2020197787A1 (en) 2019-03-22 2020-10-01 Becton, Dickinson And Company Spectral unmixing of fluorescence imaging using radiofrequency-multiplexed excitation data
WO2020197785A1 (en) 2019-03-27 2020-10-01 Becton, Dickinson And Company Systems for cell sorting based on frequency-encoded images and methods of use thereof
WO2020205200A1 (en) * 2019-03-29 2020-10-08 Becton, Dickinson And Company Parameters for use in particle discrimination
US11275026B2 (en) * 2019-05-14 2022-03-15 Becton, Dickinson And Company Phase-calibration for imaging flow cytometry
BR112021019318A2 (pt) 2019-05-30 2021-12-14 Becton Dickinson Co Correção de fase de sinais multiplexados de frequência de rádio
JP7561822B2 (ja) 2019-07-10 2024-10-04 ベクトン・ディキンソン・アンド・カンパニー 細胞選別分類を調整するための再構成可能な集積回路
JP7663587B2 (ja) 2020-01-31 2025-04-16 ベクトン・ディキンソン・アンド・カンパニー 訓練ゲートを調整してフローサイトメータデータに適応させるための方法およびシステム
US11674877B2 (en) * 2020-02-03 2023-06-13 LASE Innovation Inc. Apparatus and method for cyclic flow cytometry using particularized cell identification
US11879827B2 (en) * 2020-04-29 2024-01-23 Becton, Dickinson And Company Methods for modulation and synchronous detection in a flow cytometer and systems for same
US11674879B2 (en) 2020-05-06 2023-06-13 Becton, Dickinson And Company Methods and systems for characterizing spillover spreading in flow cytometer data
EP4154365A4 (en) 2020-05-19 2023-11-08 Becton, Dickinson and Company METHOD FOR MODULATING AN INTENSITY PROFILE OF A LASER BEAM AND SYSTEMS THEREFOR
CN115667890B (zh) * 2020-05-20 2024-02-02 Ysi公司 基于空间梯度的荧光计
EP4168774A4 (en) 2020-06-19 2023-12-27 Becton, Dickinson and Company FLOW CYTOMETER WITH ADJUSTABLE POSITION OFFSET SORTING DEFLECTION PLATES AND METHOD OF USE THEREOF
WO2021262359A1 (en) 2020-06-24 2021-12-30 Becton, Dickinson And Company Flow cytometric droplet dispensing systems and methods for using the same
CN116171383A (zh) 2020-06-26 2023-05-26 贝克顿·迪金森公司 用于照射流体流中的样品的双激励光束及其使用方法
CN116348754A (zh) 2020-09-22 2023-06-27 贝克顿·迪金森公司 在流式细胞仪中连续测量基线噪声的方法及其系统
CN116981927A (zh) 2020-10-20 2023-10-31 贝克顿·迪金森公司 包括倾斜光束整形光学部件的流式细胞仪及其使用方法
GB2600719B (en) * 2020-11-05 2023-10-11 Oxford Nanoimaging Ltd Fluorescence assay
EP4247255A4 (en) 2020-11-19 2024-05-22 Becton, Dickinson and Company Method for optimal scaling of cytometry data for machine learning analysis and systems for same
CN116897281A (zh) 2021-01-13 2023-10-17 贝克顿·迪金森公司 包括光收集增强器的流式细胞仪及其使用方法
WO2022164672A1 (en) * 2021-01-27 2022-08-04 Becton, Dickinson And Company Flow cytometers including fiber optic light collectors, and methods of use thereof
CN117098980A (zh) 2021-02-04 2023-11-21 贝克顿·迪金森公司 集成光询问模块及其使用方法
CN117015695A (zh) 2021-03-24 2023-11-07 贝克顿·迪金森公司 封闭系统分选流式细胞仪适配器及其使用方法
US12313524B2 (en) 2021-05-14 2025-05-27 Becton, Dickinson And Company Systems for detecting light by spectral discrimination and methods for using same
CN113252142B (zh) * 2021-05-18 2022-04-19 西南科技大学 一种密闭容器中白酒液位的非接触式测量系统及方法
WO2023287568A1 (en) * 2021-07-13 2023-01-19 Becton, Dickinson And Company Methods for determining a photodetector gain correction factor for optical signals in a flow cytometer
US12467845B2 (en) 2021-08-10 2025-11-11 Becton, Dickinson And Company Clamps for operably coupling an optical component to a mounting block, and methods and systems for using the same
US12135274B2 (en) 2021-08-10 2024-11-05 Becton, Dickinson And Company Outlet fittings for reducing bubbles at the interface with a flow cell, and flow cytometers and methods using the same
AU2022352559A1 (en) 2021-09-21 2024-03-21 Becton, Dickinson And Company Baseline restoration circuit
CA3230701A1 (en) 2021-11-17 2023-05-25 Keegan Owsley Methods for dynamic real-time adjustment of a data acquisition parameter in a flow cytometer
WO2023096906A1 (en) 2021-11-24 2023-06-01 Becton, Dickinson And Company Integrated flow cytometry data quality control
CN116337699B (zh) * 2021-12-24 2025-12-12 晋城三赢精密电子有限公司 颗粒物光学检测系统及方法
CN114441418A (zh) * 2022-01-28 2022-05-06 天津凌视科技有限公司 用于高速流动微粒的成像系统、成像方法及可读存储介质
KR102553905B1 (ko) * 2022-08-24 2023-07-10 주식회사 팍스웰 세포 분석을 위한 바이오 칩
CN120936859A (zh) * 2023-02-14 2025-11-11 贝克顿·迪金森公司 用于确定用于检测样本颗粒的数据过滤器的方法及使用其的系统和方法
US20240312191A1 (en) 2023-03-14 2024-09-19 Becton, Dickinson And Company Methods for determining image filters for classifying particles of a sample and systems and methods for using same
US20240344983A1 (en) 2023-03-30 2024-10-17 Becton, Dickinson And Company Methods and systems for visualizing spectral signatures
US20240377307A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods and systems for classifying analyte data into clusters
US20240377325A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods for assessing cell mitochondrial morphology and systems for same
US20240377311A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods for image-based detection and sorting and systems for same
DE102023113321A1 (de) 2023-05-22 2024-11-28 Kurz Elektronik Gmbh Vorrichtung zur Erfassung und Auswertung von Partikel
US20250085212A1 (en) 2023-09-07 2025-03-13 Becton, Dickinson And Company Integrated Optofluidic Interrogation Modules, Flow Cytometers Including the Same, and Methods of Use Thereof
US20250137905A1 (en) 2023-10-27 2025-05-01 Becton, Dickinson And Company Fluid Supply Systems Having a Flow Control Circuit and Single Fluidic Connection, and Methods of Use Thereof
WO2025137955A1 (zh) * 2023-12-27 2025-07-03 深圳华大智造科技股份有限公司 光学整形系统及相关设备与方法
US20250237657A1 (en) 2024-01-18 2025-07-24 Becton, Dickinson And Company Methods for spectrally resolving fluorophores of a sample by generalized least squares and systems for same
US20250244333A1 (en) 2024-01-31 2025-07-31 Becton, Dickinson And Company Cell viability compensation (cvc) beads for flow cytometry applications and methods of using the same
US20250297940A1 (en) 2024-03-25 2025-09-25 Becton, Dickinson And Company Methods and systems for classifying analyte data
US20250308080A1 (en) 2024-04-02 2025-10-02 Becton, Dickinson And Company Methods for label-free cell sorting and systems for same
US20250314573A1 (en) 2024-04-05 2025-10-09 Becton, Dickinson And Company Gate/population naming in flow cytometry data analysis based on geometry and data distribution
US20250314577A1 (en) 2024-04-09 2025-10-09 Becton, Dickinson And Company Methods for assessing cell nuclei morphology and systems for same
US20250321179A1 (en) 2024-04-10 2025-10-16 Becton, Dickinson And Company Quantitative flow cytometry light scatter detector alignment
US20250341455A1 (en) 2024-05-03 2025-11-06 Becton, Dickinson And Company Flow cells having an optimized flow channel geometry, flow cytometers including the same, and methods of use thereof
US20250369861A1 (en) 2024-05-31 2025-12-04 Becton, Dickinson And Company Methods and systems for singlet discrimination in flow cytometry data and systems for same
US20250377284A1 (en) 2024-06-11 2025-12-11 Becton, Dickinson And Company Flow cells having optimized high voltage electrodes, flow cytometers including the same, and methods of use thereof
US20250382585A1 (en) 2024-06-13 2025-12-18 Nippon Becton Dickinson Company Ltd Tumor and tissue preservation reagent

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101283071B1 (ko) 2005-07-15 2013-07-05 바이오비질런트 시스템즈 인코포레이티드 병원체 및 입자 검출기 시스템 및 방법
JP2016504598A (ja) * 2013-01-09 2016-02-12 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 無線周波数多重励起を用いた蛍光イメージングのための装置及び方法

Family Cites Families (198)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6295A (en) 1849-04-10 Improvement in the manufacture of twine
US168A (en) 1837-04-17 Improvement in fire-arms
US3380584A (en) 1965-06-04 1968-04-30 Atomic Energy Commission Usa Particle separator
US3854050A (en) 1973-09-11 1974-12-10 Department Of Health Education High precision fluorometer for measuring enzymatic substrates in tissue
US3960449A (en) 1975-06-05 1976-06-01 The Board Of Trustees Of Leland Stanford Junior University Measurement of angular dependence of scattered light in a flowing stream
US4347935A (en) 1979-05-16 1982-09-07 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for electrostatically sorting biological cells
US4667830A (en) 1981-06-15 1987-05-26 The Board Of Trustees Of The Leland Stanford Junior University Method and means for sorting individual particles into containers for culturing, cloning, analysis, or the like
US4545677A (en) * 1984-03-05 1985-10-08 Becton, Dickinson And Company Prismatic beam expander for light beam shaping in a flow cytometry apparatus
US4770992A (en) 1985-11-27 1988-09-13 Den Engh Gerrit J Van Detection of specific DNA sequences by flow cytometry
DE3610396A1 (de) 1986-03-27 1987-10-01 Wella Ag Mittel und verfahren zur oxidativen faerbung von haaren
US4704891A (en) 1986-08-29 1987-11-10 Becton, Dickinson And Company Method and materials for calibrating flow cytometers and other analysis instruments
US5245318A (en) 1987-07-24 1993-09-14 Canon Kabushiki Kaisha Particle analyzing apparatus having pressure control system
US5040890A (en) 1987-11-25 1991-08-20 Becton, Dickinson And Company Sheathed particle flow controlled by differential pressure
US5047321A (en) 1988-06-15 1991-09-10 Becton Dickinson & Co. Method for analysis of cellular components of a fluid
US5030002A (en) 1989-08-11 1991-07-09 Becton, Dickinson And Company Method and apparatus for sorting particles with a moving catcher tube
JPH0810306B2 (ja) 1990-01-26 1996-01-31 大日本スクリーン製造株式会社 光ビームの偏向制御方法
JPH0427866A (ja) * 1990-05-23 1992-01-30 Omron Corp 細胞選別装置
US5204884A (en) * 1991-03-18 1993-04-20 University Of Rochester System for high-speed measurement and sorting of particles
WO1992007245A1 (en) 1990-10-10 1992-04-30 The University Of Maryland Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry
WO1992013265A1 (en) * 1991-01-24 1992-08-06 The University Of Maryland Method and apparatus for multi-dimensional phase fluorescence lifetime imaging
EP0515211A3 (en) 1991-05-23 1993-04-07 Becton Dickinson And Company Apparatus and method for phase resolved fluorescence lifetimes of independent and varying amplitude pulses
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
EP0554447B1 (en) 1991-08-28 1997-04-09 Becton, Dickinson and Company Gravitational attractor engine for adaptively autoclustering n-dimensional data streams
US5309912A (en) 1991-11-08 1994-05-10 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multidimensional imaging using a single point detector for a phase encoded modulated optical carrier
US5192870A (en) 1992-01-14 1993-03-09 International Business Machines Corporation Optical submicron aerosol particle detector
US5255257A (en) 1992-03-04 1993-10-19 Lasertape Systems, Inc. Frequency, phase and amplitude control apparatus and method for acousto-optic deflector optimization
US6057814A (en) 1993-05-24 2000-05-02 Display Science, Inc. Electrostatic video display drive circuitry and displays incorporating same
US5296911A (en) 1992-07-16 1994-03-22 Schiapparelli Biosystems, Inc. Optical test system for a chemical analyzer
US5270548A (en) * 1992-07-31 1993-12-14 The United States Of America As Represented By The United States Department Of Energy Phase-sensitive flow cytometer
US5293213A (en) 1992-08-12 1994-03-08 Klein Uwe K A Utilization of a modulated laser beam in heterodyne interferometry
JPH06186156A (ja) * 1992-10-21 1994-07-08 Toa Medical Electronics Co Ltd 粒子分析装置
US5464581A (en) 1993-08-02 1995-11-07 The Regents Of The University Of California Flow cytometer
US5483469A (en) 1993-08-02 1996-01-09 The Regents Of The University Of California Multiple sort flow cytometer
US5489977A (en) * 1993-08-11 1996-02-06 Texaco Inc. Photomeric means for monitoring solids and fluorescent material in waste water using a falling stream water sampler
US5700692A (en) 1994-09-27 1997-12-23 Becton Dickinson And Company Flow sorter with video-regulated droplet spacing
US5602039A (en) 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5643796A (en) 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
US5608519A (en) * 1995-03-20 1997-03-04 Gourley; Paul L. Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells
US5620842A (en) 1995-03-29 1997-04-15 Becton Dickinson And Company Determination of the number of fluorescent molecules on calibration beads for flow cytometry
JPH0961858A (ja) 1995-08-28 1997-03-07 Minolta Co Ltd 音響光学光走査装置
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US5968737A (en) 1996-11-12 1999-10-19 The University Of Mississippi Method of identifying inhibitors of glutathione S-transferase (GST) gene expression
JPH10148778A (ja) 1996-11-18 1998-06-02 Minolta Co Ltd マルチビーム発生装置
DE19721881C2 (de) 1997-05-26 1999-05-20 Bosch Gmbh Robert Interferometrische Meßvorrichtung
DE19721882C2 (de) 1997-05-26 1999-04-29 Bosch Gmbh Robert Interferometrische Meßvorrichtung
US6031852A (en) 1997-05-30 2000-02-29 The Regents Of The University Of California Rapid acoustooptic tuner and phase-shifter
US6016196A (en) 1997-06-17 2000-01-18 Massachusetts Institute Of Technology Multiple beam pair optical imaging
US6236454B1 (en) 1997-12-15 2001-05-22 Applied Materials, Inc. Multiple beam scanner for an inspection system
US6821740B2 (en) 1998-02-25 2004-11-23 Becton, Dickinson And Company Flow cytometric methods for the concurrent detection of discrete functional conformations of PRB in single cells
US6642018B1 (en) * 1998-03-27 2003-11-04 Oncosis Llc Method for inducing a response in one or more targeted cells
US6592822B1 (en) 1998-05-14 2003-07-15 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
AU5850499A (en) * 1998-10-01 2000-04-26 2C A/S An apparatus for determining the position of an object
US6271924B1 (en) 1998-12-29 2001-08-07 Bryan Kok Ann Ngoi Noncontact acoustic optic scanning laser vibrometer for determining the difference between an object and a reference surface
AU5760500A (en) 1999-06-23 2001-01-09 Patrick Toomey Water detection and source identification methods for structures using electromagnetic radiation spectroscopy
US6396069B1 (en) 1999-06-25 2002-05-28 Macpherson David C. Topographer for real time ablation feedback having synthetic wavelength generators
US6372506B1 (en) 1999-07-02 2002-04-16 Becton, Dickinson And Company Apparatus and method for verifying drop delay in a flow cytometer
US6813017B1 (en) 1999-10-20 2004-11-02 Becton, Dickinson And Company Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
US7300457B2 (en) 1999-11-19 2007-11-27 Advanced Bio Prosthetic Surfaces, Ltd. Self-supporting metallic implantable grafts, compliant implantable medical devices and methods of making same
US6295168B1 (en) 1999-12-15 2001-09-25 International Business Machines Corporation Refractive optical system that converts a laser beam to a collimated flat-top beam
US6809804B1 (en) 2000-05-11 2004-10-26 Becton, Dickinson And Company System and method for providing improved event reading and data processing capabilities in a flow cytometer
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6778263B2 (en) 2000-08-25 2004-08-17 Amnis Corporation Methods of calibrating an imaging system using calibration beads
JP2002296178A (ja) 2001-03-30 2002-10-09 Shimadzu Corp フローセル検出器
US6636623B2 (en) 2001-08-10 2003-10-21 Visiongate, Inc. Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease
US6683314B2 (en) 2001-08-28 2004-01-27 Becton, Dickinson And Company Fluorescence detection instrument with reflective transfer legs for color decimation
FR2830629B1 (fr) 2001-10-04 2004-01-09 Commissariat Energie Atomique Procede et dispositif de prelevement d'une partie d'un faisceau lumineux, notamment pour appareil d'analyse de fluorescence
DE20216583U1 (de) 2001-12-20 2003-01-23 Leica Microsystems Heidelberg Gmbh, 68165 Mannheim Mikroskop und Durchflusszytometer
US6868347B2 (en) 2002-03-19 2005-03-15 The Regents Of The University Of California System for real time, non-invasive metrology of microfluidic chips
US20040002154A1 (en) 2002-04-19 2004-01-01 Palsson Bernhard O. Methods for preparing libraries of unique tags and related screening methods
US20060014212A1 (en) * 2002-05-10 2006-01-19 Epitome Biosystems, Inc. Proteome epitope tags and methods of use thereof in protein modification analysis
DE10225838B4 (de) 2002-06-11 2021-10-21 Leica Microsystems Cms Gmbh Verfahren zur Scanmikroskopie, Scanmikroskop und Vorrichtung zum Codieren eines Beleuchtungslichtstrahles
US7201875B2 (en) 2002-09-27 2007-04-10 Becton Dickinson And Company Fixed mounted sorting cuvette with user replaceable nozzle
WO2004088574A1 (en) * 2003-04-02 2004-10-14 Amersham Biosciences Uk Limited Method of, and computer software for, classification of cells into subpopulations
EP1668355A4 (en) 2003-08-28 2011-11-09 Celula Inc METHODS AND APPARATUS FOR SORTING CELLS USING AN OPTICAL SWITCH IN A MICROFLUIDIC CHANNEL NETWORK
US7803624B2 (en) 2003-09-30 2010-09-28 Cytyc Corporation Automated cytological sample classification
US7103402B2 (en) 2003-10-02 2006-09-05 Ut-Battelle, Llc Advanced synchronous luminescence imaging for chemical and medical diagnostics
US7590991B2 (en) 2003-10-09 2009-09-15 Terayon Communication Systems, Inc. Method and apparatus for determining channel to which a TV or VCR is tuned
EP3009815B1 (en) 2003-10-27 2022-09-07 The General Hospital Corporation Method and apparatus for performing optical imaging using frequency-domain interferometry
DE10357584B4 (de) 2003-12-08 2006-06-14 Leica Microsystems Cms Gmbh Verfahren zum Trennen unterschiedlicher Emissionswellenlängen in einem Scanmikroskop
JP4304120B2 (ja) 2004-04-30 2009-07-29 ベイバイオサイエンス株式会社 生物学的粒子をソーティングする装置及び方法
US7133182B2 (en) 2004-06-07 2006-11-07 Electro Scientific Industries, Inc. AOM frequency and amplitude modulation techniques for facilitating full beam extinction in laser systems
US20050287680A1 (en) 2004-06-25 2005-12-29 Srivatsa Venkatasubbarao Multianalyte assay method
CA2591200A1 (en) * 2004-12-17 2006-06-22 Luminex Corporation Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore
US20060149479A1 (en) 2004-12-30 2006-07-06 Art, Advanced Research Technologies Inc. Method for improving fluorescence image contrast
CN101799398B (zh) 2005-08-02 2013-06-05 卢米尼克斯股份有限公司 用于分类微粒的方法和设备
JP4756948B2 (ja) * 2005-08-08 2011-08-24 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
WO2007041382A1 (en) 2005-09-29 2007-04-12 General Hospital Corporation Arrangements and methods for providing multimodality microscopic imaging of one or more biological structures
JP5203951B2 (ja) 2005-10-14 2013-06-05 ザ ジェネラル ホスピタル コーポレイション スペクトル及び周波数符号化蛍光画像形成
GB0525072D0 (en) 2005-12-09 2006-01-18 Enigma Diagnostics Ltd Fluorescence-based detection methods and apparatus
EP2899561B1 (en) 2006-02-17 2021-04-28 Regents of the University of Minnesota MRI method for generating a map of the transmit RF field for each coil of a RF coil array
US20090323061A1 (en) 2006-02-28 2009-12-31 Lukas Novotny Multi-color hetereodyne interferometric apparatus and method for sizing nanoparticles
JP2007285999A (ja) 2006-04-20 2007-11-01 Furukawa Electric Co Ltd:The 光測定装置
EP2024893B1 (en) * 2006-05-12 2015-03-04 Janssen Diagnostics, LLC A laser illumination system in fluorescent microscopy
JP5133600B2 (ja) 2006-05-29 2013-01-30 オリンパス株式会社 レーザ走査型顕微鏡および顕微鏡観察方法
ATE440301T1 (de) 2006-05-29 2009-09-15 Olympus Corp Laserscan-mikroskop und mikroskopisches überwachungsverfahren
JP2008007652A (ja) 2006-06-29 2008-01-17 Fujifilm Corp アゾ色素、感熱転写記録用インクシート、感熱転写記録方法、カラートナー、インクジェット用インクおよびカラーフィルタ
US20100032584A1 (en) * 2006-08-18 2010-02-11 Macquarie University Tiime gated fluorescent flow cytometer
US7697885B2 (en) 2006-09-15 2010-04-13 Aeroflex High Speed Test Solutions, Inc. Multi-band jammer
US7804594B2 (en) 2006-12-29 2010-09-28 Abbott Laboratories, Inc. Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US7400457B1 (en) 2007-01-04 2008-07-15 Stockeryale Canada Inc. Rectangular flat-top beam shaper
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
US8101426B2 (en) * 2007-03-02 2012-01-24 Icyt Mission Technology, Inc. System and method for the measurement of multiple fluorescence emissions in a flow cytometry system
US8290625B2 (en) 2007-04-04 2012-10-16 Beckman Coulter, Inc. Flow cytometer sorter
JP5619344B2 (ja) 2007-05-04 2014-11-05 マツクス−プランク−ゲゼルシャフト ツール フエルデルング デル ヴイツセンシャフテン エー フアウ モノリシックマイクロ共振器を使用した光周波数コム発生のための装置と方法
US7982944B2 (en) 2007-05-04 2011-07-19 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method and apparatus for optical frequency comb generation using a monolithic micro-resonator
WO2009005579A1 (en) 2007-06-14 2009-01-08 The General Hospital Corporation High affinity fluorochrome binding peptides
DK2171098T3 (en) 2007-06-29 2018-05-22 Becton Dickinson Co PROCEDURES FOR EXTRACTION AND CLEANING COMPONENTS IN BIOLOGICAL SAMPLES
US8848199B2 (en) 2007-07-10 2014-09-30 Massachusetts Institute Of Technology Tomographic phase microscopy
JP4509163B2 (ja) 2007-10-26 2010-07-21 ソニー株式会社 微小粒子の測定方法
JP4389991B2 (ja) * 2007-10-26 2009-12-24 ソニー株式会社 微小粒子の光学的測定方法及び光学的測定装置
US8159670B2 (en) 2007-11-05 2012-04-17 Abbott Laboratories Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US9451884B2 (en) * 2007-12-13 2016-09-27 Board Of Trustees Of The University Of Arkansas Device and method for in vivo detection of clots within circulatory vessels
GB0800333D0 (en) 2008-01-09 2008-02-20 Ucl Business Plc Beam deflection apparatus and methods
US20090219607A1 (en) 2008-01-17 2009-09-03 Baylor College Of Medicine Method and apparatus for enhanced resolution microscopy of living biological nanostructures
US7920261B2 (en) 2008-02-11 2011-04-05 Massachusetts Institute Of Technology Method and apparatus for detecting and discriminating particles in a fluid
US8565861B2 (en) 2008-05-02 2013-10-22 Olympus Corporation Optical inspection device, electromagnetic wave detection method, electromagnetic wave detection device, organism observation method, microscope, endoscope, and optical tomographic image generation device
US8140300B2 (en) 2008-05-15 2012-03-20 Becton, Dickinson And Company High throughput flow cytometer operation with data quality assessment and control
WO2009149733A1 (en) * 2008-06-13 2009-12-17 Embl Heidelberg Next generation flow cytometer sorter
US8184279B2 (en) 2008-06-16 2012-05-22 The Regents Of The University Of Colorado, A Body Corporate Fourier domain sensing
CN102150035A (zh) * 2008-09-19 2011-08-10 三井造船株式会社 采用强度调制激光的荧光检测装置和荧光检测方法
US10908084B2 (en) 2008-10-14 2021-02-02 Timothy M. Ragan Devices and methods for direct-sampling analog time-resolved detection
US8440952B2 (en) 2008-11-18 2013-05-14 The Regents Of The University Of California Methods for optical amplified imaging using a two-dimensional spectral brush
US20110275534A1 (en) 2008-12-05 2011-11-10 The Ohio State University MicroRNA-Based Methods and Compositions for the Diagnosis, Prognosis and Treatment of Ovarian Cancer Using a Real-Time PCR Platform
US8233146B2 (en) 2009-01-13 2012-07-31 Becton, Dickinson And Company Cuvette for flow-type particle analyzer
US8885165B2 (en) * 2009-01-22 2014-11-11 Mitsui Engineering & Shipbuilding Co., Ltd. Fluorescence detecting device and fluorescence detecting method
WO2010084720A1 (ja) 2009-01-23 2010-07-29 三井造船株式会社 蛍光検出装置及び蛍光検出方法
JP4564566B2 (ja) * 2009-02-13 2010-10-20 三井造船株式会社 蛍光検出装置及び蛍光検出方法
JP4620786B2 (ja) * 2009-02-17 2011-01-26 三井造船株式会社 蛍光検出方法、蛍光検出装置及びプログラム
US9221886B2 (en) * 2009-04-28 2015-12-29 President And Fellows Of Harvard College Supercharged proteins for cell penetration
TWI594828B (zh) * 2009-05-28 2017-08-11 伊雷克托科學工業股份有限公司 應用於雷射處理工件中的特徵的聲光偏轉器及相關雷射處理方法
EP2264168B1 (de) 2009-06-18 2014-12-17 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren
JP5612278B2 (ja) 2009-06-23 2014-10-22 パナソニック株式会社 三次元形状造形物の製造方法およびその製造装置
EP2267430A1 (de) * 2009-06-24 2010-12-29 Masterrind GmbH Vorrichtung und Verfahren zur Selektion von Partikeln
WO2011023593A1 (en) 2009-08-24 2011-03-03 INSERM (Institut National de la Santé et de la Recherche Médicale) Method of and apparatus for imaging a cellular sample
JP5534214B2 (ja) * 2009-10-05 2014-06-25 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
GB0919854D0 (en) * 2009-11-12 2009-12-30 Stfc Science & Technology Detecting species in a dilute medium
JP5649828B2 (ja) 2010-02-03 2015-01-07 オリンパス株式会社 レーザ顕微鏡装置
WO2011097032A1 (en) 2010-02-05 2011-08-11 Cytonome/St, Llc Multiple flow channel particle analysis system
JP2011163787A (ja) 2010-02-05 2011-08-25 Sony Corp 微小粒子分析装置及び微小粒子分析方法
JP5551477B2 (ja) 2010-03-15 2014-07-16 オリンパス株式会社 光源装置およびレーザ走査型顕微鏡装置
JP5437148B2 (ja) 2010-04-23 2014-03-12 ベイバイオサイエンス株式会社 フローサイトメータおよびセルソータ
US9101573B2 (en) 2010-05-04 2015-08-11 Virginia Tech Intellectual Properties, Inc. Lanthionine synthetase component C-like proteins as molecular targets for preventing and treating diseases and disorders
JP2012008055A (ja) 2010-06-25 2012-01-12 Olympus Corp 画像解析方法および画像解析装置
US8384045B2 (en) 2010-07-01 2013-02-26 Sony Corporation Minute particle analyzing device and method
US8574838B2 (en) 2010-07-14 2013-11-05 University Of Medicine And Dentistry Of New Jersey Methods and kits for miRNA isolation and quantitation
US8528427B2 (en) 2010-10-29 2013-09-10 Becton, Dickinson And Company Dual feedback vacuum fluidics for a flow-type particle analyzer
WO2012060163A1 (ja) * 2010-11-01 2012-05-10 財団法人神奈川科学技術アカデミー 細胞分析装置
DE102010044013A1 (de) 2010-11-16 2012-05-16 Carl Zeiss Microimaging Gmbh Tiefenauflösungsgesteigerte Mikroskopie
JP2014503195A (ja) 2010-11-16 2014-02-13 1087 システムズ インコーポレイテッド 生細胞の識別および選別用システム
US9066657B2 (en) 2010-11-23 2015-06-30 General Electric Company Methods and systems of optical imaging for target detection in a scattering medium
US10481069B2 (en) 2011-01-03 2019-11-19 Cytonome/St, Llc Method and apparatus for monitoring and optimizing microfluidic particle sorting
WO2012106294A1 (en) * 2011-02-04 2012-08-09 Cytonome/St, Llc Particle sorting apparatus and method
WO2012127907A1 (ja) 2011-03-18 2012-09-27 独立行政法人理化学研究所 非線形光学顕微鏡および非線形光学顕微鏡法
US8990047B2 (en) 2011-03-21 2015-03-24 Becton, Dickinson And Company Neighborhood thresholding in mixed model density gating
JP5620319B2 (ja) 2011-03-23 2014-11-05 オリンパス株式会社 顕微鏡
CN103649294B (zh) 2011-04-29 2016-05-18 贝克顿·迪金森公司 多路分类系统和方法
EP2710694B1 (en) 2011-05-16 2018-07-04 Oewaves, Inc. Generation of single optical tone, rf oscillation signal and optical comb in a triple-oscillator device based on nonlinear optical resonator
JP6035329B2 (ja) 2011-05-26 2016-11-30 ザ ジェネラル ホスピタル コーポレイション 光学的トロンボエラストグラフィーシステム及び血液凝固基準の評価方法
WO2013011001A1 (en) 2011-07-19 2013-01-24 Ovizio Imaging Systems N.V. A method and system for detecting and/or classifying cancerous cells in a cell sample
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
EP2760413B1 (en) 2011-09-30 2017-12-06 Becton Dickinson and Company Fluid exchange methods and devices
JP5870851B2 (ja) 2012-05-29 2016-03-01 ソニー株式会社 情報処理装置、情報処理方法、及びプログラム
JP2014002470A (ja) 2012-06-15 2014-01-09 Ricoh Co Ltd 処理装置、処理システム、出力方法およびプログラム
US9168568B2 (en) * 2012-08-01 2015-10-27 Owl biomedical, Inc. Particle manipulation system with cytometric confirmation
WO2014031985A1 (en) 2012-08-24 2014-02-27 The Trustees Of Dartmouth College Method and apparatus for magnetic susceptibility tomography, magnetoencephalography, and taggant or contrast agent detection
US8723104B2 (en) 2012-09-13 2014-05-13 City University Of Hong Kong Methods and means for manipulating particles
WO2014062719A2 (en) 2012-10-15 2014-04-24 Nanocellect Biomedical, Inc. Systems, apparatus, and methods for sorting particles
US9862987B2 (en) 2013-01-16 2018-01-09 The Regents Of The University Of California Label free molecular detection methods, systems and devices
ES2931314T3 (es) 2013-02-01 2022-12-28 Becton Dickinson Co Métodos y sistemas para evaluar el comportamiento de una muestra en un citómetro de flujo
EP4134656A1 (en) 2013-03-14 2023-02-15 Cytonome/ST, LLC Assemblies and methods for reducing optical crosstalk in particle processing systems
US9964968B2 (en) 2013-03-14 2018-05-08 Cytonome/St, Llc Operatorless particle processing systems and methods
GB201305317D0 (en) 2013-03-22 2013-05-08 Iles Raymond K Prenatal screening for fetal abnormalities and disorders of pregnancy
JP2016521362A (ja) 2013-04-12 2016-07-21 ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company 細胞分取のための自動セットアップ
US9372143B2 (en) * 2013-05-15 2016-06-21 Captl Llc Scanning image flow cytometer
JP6136843B2 (ja) * 2013-10-17 2017-05-31 ソニー株式会社 粒子分取装置、粒子分取方法及びプログラム
JP6257312B2 (ja) 2013-12-24 2018-01-10 オリンパス株式会社 顕微鏡システム
US9538926B2 (en) 2013-12-26 2017-01-10 Fundacio Institut De Ciencies Fotoniques Speckle contrast optical tomography
CN103762487B (zh) 2014-01-04 2016-05-25 天津奇谱光电技术有限公司 一种具有双输出光束的可调谐激光器
JP6214402B2 (ja) 2014-01-07 2017-10-18 オリンパス株式会社 光刺激装置および顕微鏡システム
US9207066B2 (en) * 2014-02-14 2015-12-08 Palo Alto Research Center Incorporated Spatial modulation of light to determine dimensional characteristics of objects in a flow path
US10451482B2 (en) * 2014-02-14 2019-10-22 Palo Alto Research Center Incorporated Determination of color characteristics of objects using spatially modulated light
US9528925B2 (en) * 2014-02-14 2016-12-27 Palo Alto Research Center Incorporated Spatial modulation of light to determine object position
ES2959506T3 (es) 2014-03-18 2024-02-26 Univ California Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método
WO2015168026A2 (en) 2014-04-28 2015-11-05 The Broad Institute, Inc. Method for label-free image cytometry
US10267736B2 (en) * 2014-09-30 2019-04-23 The Regents Of The University Of California Imaging flow cytometer using spatial-temporal transformation
US10451953B2 (en) 2014-11-12 2019-10-22 Orbotech Ltd. Acousto-optic deflector with multiple output beams
WO2017053592A1 (en) 2015-09-23 2017-03-30 The Regents Of The University Of California Deep learning in label-free cell classification and machine vision extraction of particles
KR102600559B1 (ko) 2015-10-13 2023-11-10 벡톤 디킨슨 앤드 컴퍼니 다중모드 형광 이미징 유동 세포 계측 시스템
US10337997B2 (en) 2015-10-15 2019-07-02 Woods Hole Oceanographic Institution System for rapid assessment of water quality and harmful algal bloom toxins
JP7033082B2 (ja) 2016-02-05 2022-03-09 ナノビュー バイオサイエンシズ インコーポレイテッド 表面マーカーを有するエキソソームの検出
CN109564151B (zh) 2016-03-17 2023-01-24 贝克顿·迪金森公司 使用高通量荧光流式细胞仪进行细胞分选
WO2017201540A1 (en) 2016-05-20 2017-11-23 Techcyte, Inc. Machine learning classification of particles or substances in digital microscopy images
CN115639134A (zh) 2016-06-10 2023-01-24 加利福尼亚大学董事会 基于图像的细胞分选系统和方法
US10006852B2 (en) 2016-09-13 2018-06-26 Becton, Dickinson And Company Flow cytometer with optical equalization
CN111344550A (zh) 2017-10-09 2020-06-26 Tsi有限公司 粒子计数器部件校准
CN112105912B (zh) 2018-04-13 2025-05-23 华盛顿大学 用于单生物纳米颗粒分析的方法和设备
JP7394786B2 (ja) 2018-04-26 2023-12-08 ベクトン・ディキンソン・アンド・カンパニー 粒子分析器のための特性評価および選別
WO2020205200A1 (en) 2019-03-29 2020-10-08 Becton, Dickinson And Company Parameters for use in particle discrimination

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101283071B1 (ko) 2005-07-15 2013-07-05 바이오비질런트 시스템즈 인코포레이티드 병원체 및 입자 검출기 시스템 및 방법
JP2016504598A (ja) * 2013-01-09 2016-02-12 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 無線周波数多重励起を用いた蛍光イメージングのための装置及び方法

Also Published As

Publication number Publication date
US12078587B2 (en) 2024-09-03
CA3018065A1 (en) 2017-09-21
US20170268981A1 (en) 2017-09-21
US10620111B2 (en) 2020-04-14
WO2017161247A1 (en) 2017-09-21
ZA201806386B (en) 2020-02-26
US20240410813A1 (en) 2024-12-12
KR102641469B1 (ko) 2024-02-28
CN109564151A (zh) 2019-04-02
EP3430376B8 (en) 2024-11-27
US10324019B2 (en) 2019-06-18
EP4545936A2 (en) 2025-04-30
JP7502361B2 (ja) 2024-06-18
JP2024119909A (ja) 2024-09-03
JP2022095825A (ja) 2022-06-28
US20210356381A1 (en) 2021-11-18
US20200209140A1 (en) 2020-07-02
US20230076378A1 (en) 2023-03-09
BR112018068866A2 (pt) 2019-01-22
US20190234863A1 (en) 2019-08-01
KR20180129832A (ko) 2018-12-05
EP3430376B1 (en) 2024-10-23
US11774343B2 (en) 2023-10-03
US12313521B1 (en) 2025-05-27
EP3430376A1 (en) 2019-01-23
JP2019513236A (ja) 2019-05-23
KR20220002706A (ko) 2022-01-06
CN109564151B (zh) 2023-01-24
AU2017234815B2 (en) 2022-11-03
BR112018068866B1 (pt) 2022-11-29
KR20230003444A (ko) 2023-01-05
AU2017234815A1 (en) 2018-10-04
US20250155356A1 (en) 2025-05-15
JP7130562B2 (ja) 2022-09-05
US11105728B2 (en) 2021-08-31
EP4545936A3 (en) 2025-07-16

Similar Documents

Publication Publication Date Title
JP7502361B2 (ja) ハイスループット蛍光フローサイトメーターを用いる細胞選別
KR102381176B1 (ko) 개선된 이미지 해상도를 갖는 형광 이미징 유세포 분석법
KR102711298B1 (ko) 다중모드 형광 이미징 유동 세포 계측 시스템
US12436087B2 (en) Spectral unmixing of fluorescence imaging using radiofrequency-multiplexed excitation data

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20211223

Application number text: 1020187030001

Filing date: 20181017

A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20220106

Comment text: Request for Examination of Application

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20220411

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20221026

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20230131

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20230426

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20230427

End annual number: 3

Start annual number: 1

PG1601 Publication of registration