BR112018068866A2 - classificação de células utilizando um citômetro de fluxo de fluorescência de alto rendimento - Google Patents
classificação de células utilizando um citômetro de fluxo de fluorescência de alto rendimentoInfo
- Publication number
- BR112018068866A2 BR112018068866A2 BR112018068866A BR112018068866A BR112018068866A2 BR 112018068866 A2 BR112018068866 A2 BR 112018068866A2 BR 112018068866 A BR112018068866 A BR 112018068866A BR 112018068866 A BR112018068866 A BR 112018068866A BR 112018068866 A2 BR112018068866 A2 BR 112018068866A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- pixel
- data
- fluorescence
- employed
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 abstract 6
- 238000001506 fluorescence spectroscopy Methods 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 230000003287 optical effect Effects 0.000 abstract 3
- 230000005855 radiation Effects 0.000 abstract 3
- 230000002123 temporal effect Effects 0.000 abstract 2
- 210000003850 cellular structure Anatomy 0.000 abstract 1
- 238000000684 flow cytometry Methods 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N15/149—
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
- G01N2015/1447—Spatial selection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
Abstract
em um aspecto, a invenção trata de um método de classificação de células num sistema de citometria de fluxo é descrito, que inclui iluminação de uma célula com radiação tendo pelo menos duas frequências ópticas deslocadas uma da outra por uma radiofrequência para eliciar radiação fluorescente da célula, detectar a radiação fluorescente para gerar dados de fluorescência temporal, e processar os dados de fluorescência temporal para chegar a uma decisão de triagem referente à célula sem gerar uma imagem (isto é, uma imagem de pixel por pixel) da célula com base nos dados de fluorescência. em outras palavras, enquanto os dados de fluorescência podem conter dados de imagem que permitiriam a geração de um mapa de intensidade de fluorescência pixel por pixel, o método chega à decisão de triagem sem gerar um tal mapa. em alguns casos, a decisão de triagem pode ser feita com uma latência inferior a cerca de 100 microssegundos. em algumas modalidades, o método acima de classificar células pode ter uma resolução sub-celular, por exemplo a decisão de classificação pode ser baseada em características de um componente da célula. em outras formas de realização, em que mais de duas frequências ópticas deslocadas em frequência são empregadas, um único deslocamento de radiofrequência é empregado para separar as frequências ópticas enquanto em outras modalidades, uma pluralidade de diferentes deslocamentos de frequência de frequência é empregada.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662309806P | 2016-03-17 | 2016-03-17 | |
US62/309,806 | 2016-03-17 | ||
PCT/US2017/022936 WO2017161247A1 (en) | 2016-03-17 | 2017-03-17 | Cell sorting using a high throughput fluorescence flow cytometer |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112018068866A2 true BR112018068866A2 (pt) | 2019-01-22 |
BR112018068866B1 BR112018068866B1 (pt) | 2022-11-29 |
Family
ID=58428427
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018068866-5A BR112018068866B1 (pt) | 2016-03-17 | 2017-03-17 | Classificação de células utilizando um citômetro de fluxo de fluorescência de alto rendimento |
Country Status (10)
Country | Link |
---|---|
US (5) | US10324019B2 (pt) |
EP (1) | EP3430376A1 (pt) |
JP (2) | JP7130562B2 (pt) |
KR (3) | KR102641469B1 (pt) |
CN (1) | CN109564151B (pt) |
AU (1) | AU2017234815B2 (pt) |
BR (1) | BR112018068866B1 (pt) |
CA (1) | CA3018065A1 (pt) |
WO (1) | WO2017161247A1 (pt) |
ZA (1) | ZA201806386B (pt) |
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