AU2017234815B2 - Cell sorting using a high throughput fluorescence flow cytometer - Google Patents

Cell sorting using a high throughput fluorescence flow cytometer Download PDF

Info

Publication number
AU2017234815B2
AU2017234815B2 AU2017234815A AU2017234815A AU2017234815B2 AU 2017234815 B2 AU2017234815 B2 AU 2017234815B2 AU 2017234815 A AU2017234815 A AU 2017234815A AU 2017234815 A AU2017234815 A AU 2017234815A AU 2017234815 B2 AU2017234815 B2 AU 2017234815B2
Authority
AU
Australia
Prior art keywords
particle
fluorescence
cell
radiation
data
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017234815A
Other languages
English (en)
Other versions
AU2017234815A1 (en
Inventor
Eric Diebold
Jonathan Lin
Keegan Owsley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of AU2017234815A1 publication Critical patent/AU2017234815A1/en
Assigned to BECTON, DICKINSON AND COMPANY reassignment BECTON, DICKINSON AND COMPANY Request for Assignment Assignors: BD BIOSCIENCES
Application granted granted Critical
Publication of AU2017234815B2 publication Critical patent/AU2017234815B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1447—Spatial selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2017234815A 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer Active AU2017234815B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662309806P 2016-03-17 2016-03-17
US62/309,806 2016-03-17
PCT/US2017/022936 WO2017161247A1 (en) 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer

Publications (2)

Publication Number Publication Date
AU2017234815A1 AU2017234815A1 (en) 2018-10-04
AU2017234815B2 true AU2017234815B2 (en) 2022-11-03

Family

ID=58428427

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017234815A Active AU2017234815B2 (en) 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer

Country Status (10)

Country Link
US (7) US10324019B2 (enExample)
EP (2) EP4545936A3 (enExample)
JP (3) JP7130562B2 (enExample)
KR (3) KR102527830B1 (enExample)
CN (1) CN109564151B (enExample)
AU (1) AU2017234815B2 (enExample)
BR (1) BR112018068866B1 (enExample)
CA (1) CA3018065A1 (enExample)
WO (1) WO2017161247A1 (enExample)
ZA (1) ZA201806386B (enExample)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
JP6338597B2 (ja) 2013-01-09 2018-06-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 無線周波数多重励起を用いた蛍光イメージングのための装置及び方法
ES2959506T3 (es) 2014-03-18 2024-02-26 Univ California Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método
CN105987870A (zh) * 2015-02-10 2016-10-05 博奥生物集团有限公司 一种流式细胞分选系统及其聚焦检测方法及其流体芯片
US10921112B2 (en) * 2015-09-17 2021-02-16 Technion Research & Development Foundation Ltd. Reflectance confocal microscopy of blood cells
JP6856635B2 (ja) 2015-10-13 2021-04-07 オメガ バイオシステムズ インコーポレイテッド マルチモードの蛍光撮像フローサイトメトリシステム
AU2017234815B2 (en) 2016-03-17 2022-11-03 Becton, Dickinson And Company Cell sorting using a high throughput fluorescence flow cytometer
KR102381176B1 (ko) 2016-05-12 2022-04-01 비디 바이오사이언시스 개선된 이미지 해상도를 갖는 형광 이미징 유세포 분석법
US10006852B2 (en) 2016-09-13 2018-06-26 Becton, Dickinson And Company Flow cytometer with optical equalization
EP3579973A4 (en) 2017-02-07 2020-07-29 Nodexus Inc. MICROFLUIDIC SYSTEM WITH COMBINED ELECTRICAL AND OPTICAL DETECTION FOR HIGHLY ACCURATE PARTICLE SORTING AND METHOD THEREFOR
US20180292662A1 (en) * 2017-04-05 2018-10-11 Osela Inc. Rectangular beam shaper having monolithic body of refractive material
EP3654018B1 (en) * 2017-07-11 2023-06-21 Hamamatsu Photonics K.K. Sample observation device and sample observation method
US10593082B2 (en) 2017-07-18 2020-03-17 Becton, Dickinson And Company Dynamic display of multi-parameter quantitative biological data
US10803637B2 (en) 2017-07-18 2020-10-13 Becton, Dickinson And Company Dynamic interactive display of multi-parameter quantitative biological data
US10636182B2 (en) 2017-07-18 2020-04-28 Becton, Dickinson And Company Dynamic interactive display of multi-parameter quantitative biological data
US11099129B2 (en) 2017-09-14 2021-08-24 Brown University Spatially resolved standoff trace chemical sensing using backwards transient absorption spectroscopy
US10466173B2 (en) * 2017-10-06 2019-11-05 Wyatt Technology Corporation Optical flow cell assembly incorporating a replaceable transparent flow cell
US10444137B2 (en) 2018-01-23 2019-10-15 Cbrn International, Ltd. Bioaerosol detector having safeguards
EP3752815A4 (en) * 2018-02-16 2021-11-03 The Regents of The University of California SYSTEMS, DEVICES AND METHODS FOR THREE-DIMENSIONAL IMAGING OF MOVING PARTICLES
US20200071691A1 (en) * 2018-08-28 2020-03-05 Cellular Research, Inc. Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
EP3850080B1 (en) * 2018-09-14 2025-11-05 The Regents of the University of California Cell sorting device and method
WO2020106036A1 (en) * 2018-11-19 2020-05-28 Samsung Electronics Co., Ltd. Multimodal dust sensor
WO2020146733A1 (en) 2019-01-11 2020-07-16 Becton, Dickinson And Company Optimized sorting gates
EP3942279A4 (en) 2019-03-21 2022-12-28 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS AND METHODS OF USE THEREOF
CN119845832A (zh) 2019-03-22 2025-04-18 贝克顿·迪金森公司 使用射频多路复用激发数据对荧光成像进行光谱解混
US11493427B2 (en) 2019-03-27 2022-11-08 Becton, Dickinson And Company Systems for cell sorting based on frequency-encoded images and methods of use thereof
CN113544490B (zh) * 2019-03-29 2024-07-02 贝克顿·迪金森公司 用于颗粒辨别的参数
JP7504127B2 (ja) * 2019-05-14 2024-06-21 ベクトン・ディキンソン・アンド・カンパニー イメージングフローサイトメトリーのための位相キャリブレーション
IL287195B2 (en) 2019-05-30 2025-07-01 Becton Dickinson Co Phase correction of multiplexed radio frequency signals
EP3997439A4 (en) 2019-07-10 2023-07-19 Becton, Dickinson and Company RECONFIGURABLE INTEGRATED CIRCUITS TO ADJUST CELL SORTING CLASSIFICATION
CN115176139A (zh) 2020-01-31 2022-10-11 贝克顿·迪金森公司 用于调整训练门以适应流式细胞仪数据的方法和系统
WO2021158629A1 (en) * 2020-02-03 2021-08-12 LASE Innovation Inc. Apparatus and method for cyclic flow cytometry using particularized cell identification
US11879827B2 (en) * 2020-04-29 2024-01-23 Becton, Dickinson And Company Methods for modulation and synchronous detection in a flow cytometer and systems for same
US11674879B2 (en) 2020-05-06 2023-06-13 Becton, Dickinson And Company Methods and systems for characterizing spillover spreading in flow cytometer data
US11513058B2 (en) 2020-05-19 2022-11-29 Becton, Dickinson And Company Methods for modulating an intensity profile of a laser beam and systems for same
WO2021236720A1 (en) * 2020-05-20 2021-11-25 Ysi, Inc. Extended solid angle turbidity sensor
US11867605B2 (en) 2020-06-19 2024-01-09 Becton, Dickinson And Company Flow cytometer with adjustable positional offset sort deflection plates and methods of using the same
US11754487B2 (en) 2020-06-24 2023-09-12 Becton, Dickinson And Company Flow cytometric droplet dispensing systems and methods for using the same
EP4610627A1 (en) 2020-06-26 2025-09-03 Becton Dickinson and Company Dual excitation beams for irradiating a sample in a flow stream and methods for using same
JP7739421B2 (ja) 2020-09-22 2025-09-16 ベクトン・ディキンソン・アンド・カンパニー フローサイトメータにおけるベースラインノイズの連続測定方法およびそのためのシステム
JP2023549475A (ja) 2020-10-20 2023-11-27 ベクトン・ディキンソン・アンド・カンパニー 傾斜ビーム成形光学部品を含むフローサイトメータ、及びフローサイトメータを使用する方法
GB2600719B (en) * 2020-11-05 2023-10-11 Oxford Nanoimaging Ltd Fluorescence assay
US12306087B2 (en) 2020-11-19 2025-05-20 Becton, Dickinson And Company Method for optimal scaling of cytometry data for machine learning analysis and systems for same
CN116897281A (zh) 2021-01-13 2023-10-17 贝克顿·迪金森公司 包括光收集增强器的流式细胞仪及其使用方法
WO2022164672A1 (en) * 2021-01-27 2022-08-04 Becton, Dickinson And Company Flow cytometers including fiber optic light collectors, and methods of use thereof
US11994458B2 (en) 2021-02-04 2024-05-28 Becton, Dickinson And Company Integrated light interrogation modules and methods of use thereof
US12379301B2 (en) 2021-03-24 2025-08-05 Becton, Dickinson And Company Closed-system sorting flow cytometer adapters and methods of use thereof
EP4337933A4 (en) 2021-05-14 2024-10-23 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS BY SPECTRAL DISCRIMINATION AND METHODS OF USE THEREOF
CN113252142B (zh) * 2021-05-18 2022-04-19 西南科技大学 一种密闭容器中白酒液位的非接触式测量系统及方法
JP2024530407A (ja) * 2021-07-13 2024-08-21 ベクトン・ディキンソン・アンド・カンパニー フローサイトメータ内の光信号についての光検出器利得補正係数を決定するための方法
US12467845B2 (en) 2021-08-10 2025-11-11 Becton, Dickinson And Company Clamps for operably coupling an optical component to a mounting block, and methods and systems for using the same
US12135274B2 (en) 2021-08-10 2024-11-05 Becton, Dickinson And Company Outlet fittings for reducing bubbles at the interface with a flow cell, and flow cytometers and methods using the same
KR20240088899A (ko) 2021-09-21 2024-06-20 벡톤 디킨슨 앤드 컴퍼니 베이스라인 복원 회로
WO2023091351A1 (en) 2021-11-17 2023-05-25 Becton, Dickinson And Company Methods for dynamic real-time adjustment of a data acquisition parameter in a flow cytometer
US20230160807A1 (en) 2021-11-24 2023-05-25 Becton, Dickinson And Company Integrated Flow Cytometry Data Quality Control
TWI813127B (zh) * 2021-12-24 2023-08-21 新煒科技有限公司 顆粒物光學檢測系統及方法
CN114441418A (zh) * 2022-01-28 2022-05-06 天津凌视科技有限公司 用于高速流动微粒的成像系统、成像方法及可读存储介质
KR102553905B1 (ko) * 2022-08-24 2023-07-10 주식회사 팍스웰 세포 분석을 위한 바이오 칩
CN120936859A (zh) * 2023-02-14 2025-11-11 贝克顿·迪金森公司 用于确定用于检测样本颗粒的数据过滤器的方法及使用其的系统和方法
US20240312191A1 (en) 2023-03-14 2024-09-19 Becton, Dickinson And Company Methods for determining image filters for classifying particles of a sample and systems and methods for using same
US20240344983A1 (en) 2023-03-30 2024-10-17 Becton, Dickinson And Company Methods and systems for visualizing spectral signatures
US20240377307A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods and systems for classifying analyte data into clusters
US20240377325A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods for assessing cell mitochondrial morphology and systems for same
US20240377311A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods for image-based detection and sorting and systems for same
DE102023113321A1 (de) 2023-05-22 2024-11-28 Kurz Elektronik Gmbh Vorrichtung zur Erfassung und Auswertung von Partikel
US20250085212A1 (en) 2023-09-07 2025-03-13 Becton, Dickinson And Company Integrated Optofluidic Interrogation Modules, Flow Cytometers Including the Same, and Methods of Use Thereof
US20250137905A1 (en) 2023-10-27 2025-05-01 Becton, Dickinson And Company Fluid Supply Systems Having a Flow Control Circuit and Single Fluidic Connection, and Methods of Use Thereof
WO2025137955A1 (zh) * 2023-12-27 2025-07-03 深圳华大智造科技股份有限公司 光学整形系统及相关设备与方法
US20250237657A1 (en) 2024-01-18 2025-07-24 Becton, Dickinson And Company Methods for spectrally resolving fluorophores of a sample by generalized least squares and systems for same
US20250244333A1 (en) 2024-01-31 2025-07-31 Becton, Dickinson And Company Cell viability compensation (cvc) beads for flow cytometry applications and methods of using the same
US20250297940A1 (en) 2024-03-25 2025-09-25 Becton, Dickinson And Company Methods and systems for classifying analyte data
US20250308080A1 (en) 2024-04-02 2025-10-02 Becton, Dickinson And Company Methods for label-free cell sorting and systems for same
US20250314573A1 (en) 2024-04-05 2025-10-09 Becton, Dickinson And Company Gate/population naming in flow cytometry data analysis based on geometry and data distribution
US20250314577A1 (en) 2024-04-09 2025-10-09 Becton, Dickinson And Company Methods for assessing cell nuclei morphology and systems for same
US20250321179A1 (en) 2024-04-10 2025-10-16 Becton, Dickinson And Company Quantitative flow cytometry light scatter detector alignment
US20250341455A1 (en) 2024-05-03 2025-11-06 Becton, Dickinson And Company Flow cells having an optimized flow channel geometry, flow cytometers including the same, and methods of use thereof
US20250369861A1 (en) 2024-05-31 2025-12-04 Becton, Dickinson And Company Methods and systems for singlet discrimination in flow cytometry data and systems for same
US20250377284A1 (en) 2024-06-11 2025-12-11 Becton, Dickinson And Company Flow cells having optimized high voltage electrodes, flow cytometers including the same, and methods of use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110164246A1 (en) * 2008-06-13 2011-07-07 Embl Heidelberg Next generation flow cytometer sorter
WO2015143041A1 (en) * 2014-03-18 2015-09-24 The Regents Of The University Of California Parallel flow cytometer using radiofrequency mulitplexing

Family Cites Families (198)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US168A (en) 1837-04-17 Improvement in fire-arms
US6295A (en) 1849-04-10 Improvement in the manufacture of twine
US3380584A (en) 1965-06-04 1968-04-30 Atomic Energy Commission Usa Particle separator
US3854050A (en) 1973-09-11 1974-12-10 Department Of Health Education High precision fluorometer for measuring enzymatic substrates in tissue
US3960449A (en) 1975-06-05 1976-06-01 The Board Of Trustees Of Leland Stanford Junior University Measurement of angular dependence of scattered light in a flowing stream
US4347935A (en) 1979-05-16 1982-09-07 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for electrostatically sorting biological cells
US4667830A (en) 1981-06-15 1987-05-26 The Board Of Trustees Of The Leland Stanford Junior University Method and means for sorting individual particles into containers for culturing, cloning, analysis, or the like
US4545677A (en) * 1984-03-05 1985-10-08 Becton, Dickinson And Company Prismatic beam expander for light beam shaping in a flow cytometry apparatus
US4770992A (en) 1985-11-27 1988-09-13 Den Engh Gerrit J Van Detection of specific DNA sequences by flow cytometry
DE3610396A1 (de) 1986-03-27 1987-10-01 Wella Ag Mittel und verfahren zur oxidativen faerbung von haaren
US4704891A (en) 1986-08-29 1987-11-10 Becton, Dickinson And Company Method and materials for calibrating flow cytometers and other analysis instruments
US5245318A (en) 1987-07-24 1993-09-14 Canon Kabushiki Kaisha Particle analyzing apparatus having pressure control system
US5040890A (en) 1987-11-25 1991-08-20 Becton, Dickinson And Company Sheathed particle flow controlled by differential pressure
US5047321A (en) 1988-06-15 1991-09-10 Becton Dickinson & Co. Method for analysis of cellular components of a fluid
US5030002A (en) 1989-08-11 1991-07-09 Becton, Dickinson And Company Method and apparatus for sorting particles with a moving catcher tube
JPH0810306B2 (ja) 1990-01-26 1996-01-31 大日本スクリーン製造株式会社 光ビームの偏向制御方法
JPH0427866A (ja) * 1990-05-23 1992-01-30 Omron Corp 細胞選別装置
US5204884A (en) * 1991-03-18 1993-04-20 University Of Rochester System for high-speed measurement and sorting of particles
EP0552241B1 (en) 1990-10-10 2000-06-28 The University Of Maryland Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry
EP0568596A1 (en) * 1991-01-24 1993-11-10 The University Of Maryland Method and apparatus for multi-dimensional phase fluorescence lifetime imaging
EP0515211A3 (en) 1991-05-23 1993-04-07 Becton Dickinson And Company Apparatus and method for phase resolved fluorescence lifetimes of independent and varying amplitude pulses
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
WO1993005478A1 (en) 1991-08-28 1993-03-18 Becton, Dickinson & Company Gravitational attractor engine for adaptively autoclustering n-dimensional data streams
US5309912A (en) 1991-11-08 1994-05-10 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multidimensional imaging using a single point detector for a phase encoded modulated optical carrier
US5192870A (en) * 1992-01-14 1993-03-09 International Business Machines Corporation Optical submicron aerosol particle detector
US5255257A (en) 1992-03-04 1993-10-19 Lasertape Systems, Inc. Frequency, phase and amplitude control apparatus and method for acousto-optic deflector optimization
US6057814A (en) 1993-05-24 2000-05-02 Display Science, Inc. Electrostatic video display drive circuitry and displays incorporating same
US5296911A (en) 1992-07-16 1994-03-22 Schiapparelli Biosystems, Inc. Optical test system for a chemical analyzer
US5270548A (en) * 1992-07-31 1993-12-14 The United States Of America As Represented By The United States Department Of Energy Phase-sensitive flow cytometer
US5293213A (en) 1992-08-12 1994-03-08 Klein Uwe K A Utilization of a modulated laser beam in heterodyne interferometry
JPH06186156A (ja) * 1992-10-21 1994-07-08 Toa Medical Electronics Co Ltd 粒子分析装置
US5464581A (en) 1993-08-02 1995-11-07 The Regents Of The University Of California Flow cytometer
US5483469A (en) 1993-08-02 1996-01-09 The Regents Of The University Of California Multiple sort flow cytometer
US5489977A (en) * 1993-08-11 1996-02-06 Texaco Inc. Photomeric means for monitoring solids and fluorescent material in waste water using a falling stream water sampler
US5700692A (en) 1994-09-27 1997-12-23 Becton Dickinson And Company Flow sorter with video-regulated droplet spacing
US5602039A (en) 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5643796A (en) 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
US5608519A (en) * 1995-03-20 1997-03-04 Gourley; Paul L. Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells
US5620842A (en) 1995-03-29 1997-04-15 Becton Dickinson And Company Determination of the number of fluorescent molecules on calibration beads for flow cytometry
JPH0961858A (ja) 1995-08-28 1997-03-07 Minolta Co Ltd 音響光学光走査装置
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US5968737A (en) 1996-11-12 1999-10-19 The University Of Mississippi Method of identifying inhibitors of glutathione S-transferase (GST) gene expression
JPH10148778A (ja) 1996-11-18 1998-06-02 Minolta Co Ltd マルチビーム発生装置
DE19721881C2 (de) 1997-05-26 1999-05-20 Bosch Gmbh Robert Interferometrische Meßvorrichtung
DE19721882C2 (de) 1997-05-26 1999-04-29 Bosch Gmbh Robert Interferometrische Meßvorrichtung
US6031852A (en) 1997-05-30 2000-02-29 The Regents Of The University Of California Rapid acoustooptic tuner and phase-shifter
US6016196A (en) 1997-06-17 2000-01-18 Massachusetts Institute Of Technology Multiple beam pair optical imaging
US6236454B1 (en) 1997-12-15 2001-05-22 Applied Materials, Inc. Multiple beam scanner for an inspection system
US6821740B2 (en) 1998-02-25 2004-11-23 Becton, Dickinson And Company Flow cytometric methods for the concurrent detection of discrete functional conformations of PRB in single cells
US6642018B1 (en) * 1998-03-27 2003-11-04 Oncosis Llc Method for inducing a response in one or more targeted cells
US6592822B1 (en) 1998-05-14 2003-07-15 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
AU5850499A (en) * 1998-10-01 2000-04-26 2C A/S An apparatus for determining the position of an object
US6271924B1 (en) 1998-12-29 2001-08-07 Bryan Kok Ann Ngoi Noncontact acoustic optic scanning laser vibrometer for determining the difference between an object and a reference surface
AU5760500A (en) 1999-06-23 2001-01-09 Patrick Toomey Water detection and source identification methods for structures using electromagnetic radiation spectroscopy
US6396069B1 (en) 1999-06-25 2002-05-28 Macpherson David C. Topographer for real time ablation feedback having synthetic wavelength generators
US6372506B1 (en) 1999-07-02 2002-04-16 Becton, Dickinson And Company Apparatus and method for verifying drop delay in a flow cytometer
US6813017B1 (en) 1999-10-20 2004-11-02 Becton, Dickinson And Company Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
US7300457B2 (en) 1999-11-19 2007-11-27 Advanced Bio Prosthetic Surfaces, Ltd. Self-supporting metallic implantable grafts, compliant implantable medical devices and methods of making same
US6295168B1 (en) 1999-12-15 2001-09-25 International Business Machines Corporation Refractive optical system that converts a laser beam to a collimated flat-top beam
US6809804B1 (en) 2000-05-11 2004-10-26 Becton, Dickinson And Company System and method for providing improved event reading and data processing capabilities in a flow cytometer
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6778263B2 (en) 2000-08-25 2004-08-17 Amnis Corporation Methods of calibrating an imaging system using calibration beads
JP2002296178A (ja) 2001-03-30 2002-10-09 Shimadzu Corp フローセル検出器
US6636623B2 (en) 2001-08-10 2003-10-21 Visiongate, Inc. Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease
US6683314B2 (en) 2001-08-28 2004-01-27 Becton, Dickinson And Company Fluorescence detection instrument with reflective transfer legs for color decimation
FR2830629B1 (fr) 2001-10-04 2004-01-09 Commissariat Energie Atomique Procede et dispositif de prelevement d'une partie d'un faisceau lumineux, notamment pour appareil d'analyse de fluorescence
DE20216583U1 (de) 2001-12-20 2003-01-23 Leica Microsystems Heidelberg Gmbh, 68165 Mannheim Mikroskop und Durchflusszytometer
US6868347B2 (en) 2002-03-19 2005-03-15 The Regents Of The University Of California System for real time, non-invasive metrology of microfluidic chips
WO2003089901A2 (en) 2002-04-19 2003-10-30 Palsson Bernhard O Methods for preparing libraries of unique tags and related screening methods
US20060014212A1 (en) * 2002-05-10 2006-01-19 Epitome Biosystems, Inc. Proteome epitope tags and methods of use thereof in protein modification analysis
DE10225838B4 (de) 2002-06-11 2021-10-21 Leica Microsystems Cms Gmbh Verfahren zur Scanmikroskopie, Scanmikroskop und Vorrichtung zum Codieren eines Beleuchtungslichtstrahles
US7201875B2 (en) 2002-09-27 2007-04-10 Becton Dickinson And Company Fixed mounted sorting cuvette with user replaceable nozzle
GB2401431B (en) * 2003-04-02 2005-04-27 Amersham Biosciences Uk Ltd Method of, and computer software for, classification of cells into subpopulations
CA2536360C (en) 2003-08-28 2013-08-06 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
US7803624B2 (en) 2003-09-30 2010-09-28 Cytyc Corporation Automated cytological sample classification
US7103402B2 (en) 2003-10-02 2006-09-05 Ut-Battelle, Llc Advanced synchronous luminescence imaging for chemical and medical diagnostics
US7590991B2 (en) 2003-10-09 2009-09-15 Terayon Communication Systems, Inc. Method and apparatus for determining channel to which a TV or VCR is tuned
EP3009815B1 (en) 2003-10-27 2022-09-07 The General Hospital Corporation Method and apparatus for performing optical imaging using frequency-domain interferometry
DE10357584B4 (de) 2003-12-08 2006-06-14 Leica Microsystems Cms Gmbh Verfahren zum Trennen unterschiedlicher Emissionswellenlängen in einem Scanmikroskop
JP4304120B2 (ja) 2004-04-30 2009-07-29 ベイバイオサイエンス株式会社 生物学的粒子をソーティングする装置及び方法
US7133182B2 (en) 2004-06-07 2006-11-07 Electro Scientific Industries, Inc. AOM frequency and amplitude modulation techniques for facilitating full beam extinction in laser systems
US20050287680A1 (en) 2004-06-25 2005-12-29 Srivatsa Venkatasubbarao Multianalyte assay method
CN101084429A (zh) * 2004-12-17 2007-12-05 卢米尼克斯股份有限公司 用于增加由荧光团发射的荧光的系统、照明子系统和方法
US20060149479A1 (en) 2004-12-30 2006-07-06 Art, Advanced Research Technologies Inc. Method for improving fluorescence image contrast
EP1907818A4 (en) 2005-07-15 2012-03-14 Biovigilant Systems Inc PATHOGENIC AND PARTICULATE DETECTOR SYSTEM AND METHOD
CN101278294B (zh) 2005-08-02 2013-04-10 卢米尼克斯股份有限公司 用于分类微粒的方法、数据结构和系统
JP4756948B2 (ja) 2005-08-08 2011-08-24 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
KR20080066705A (ko) 2005-09-29 2008-07-16 더 제너럴 하스피탈 코포레이션 점진적으로 증가하는 분해능을 이용하여 하나 이상의 생물학적 샘플을 관찰 및 분석하기 위한 방법 및 장치
US7889348B2 (en) 2005-10-14 2011-02-15 The General Hospital Corporation Arrangements and methods for facilitating photoluminescence imaging
GB0525072D0 (en) 2005-12-09 2006-01-18 Enigma Diagnostics Ltd Fluorescence-based detection methods and apparatus
US7800368B2 (en) 2006-02-17 2010-09-21 Regents Of The University Of Minnesota High field magnetic resonance
US20090323061A1 (en) 2006-02-28 2009-12-31 Lukas Novotny Multi-color hetereodyne interferometric apparatus and method for sizing nanoparticles
JP2007285999A (ja) 2006-04-20 2007-11-01 Furukawa Electric Co Ltd:The 光測定装置
EP2024893B1 (en) * 2006-05-12 2015-03-04 Janssen Diagnostics, LLC A laser illumination system in fluorescent microscopy
DE602007002008D1 (de) 2006-05-29 2009-10-01 Olympus Corp Laserscan-Mikroskop und mikroskopisches Überwachungsverfahren
JP5133600B2 (ja) 2006-05-29 2013-01-30 オリンパス株式会社 レーザ走査型顕微鏡および顕微鏡観察方法
JP2008007652A (ja) 2006-06-29 2008-01-17 Fujifilm Corp アゾ色素、感熱転写記録用インクシート、感熱転写記録方法、カラートナー、インクジェット用インクおよびカラーフィルタ
US20100032584A1 (en) * 2006-08-18 2010-02-11 Macquarie University Tiime gated fluorescent flow cytometer
US7697885B2 (en) 2006-09-15 2010-04-13 Aeroflex High Speed Test Solutions, Inc. Multi-band jammer
US7804594B2 (en) 2006-12-29 2010-09-28 Abbott Laboratories, Inc. Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US7400457B1 (en) 2007-01-04 2008-07-15 Stockeryale Canada Inc. Rectangular flat-top beam shaper
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
US8101426B2 (en) * 2007-03-02 2012-01-24 Icyt Mission Technology, Inc. System and method for the measurement of multiple fluorescence emissions in a flow cytometry system
US8290625B2 (en) 2007-04-04 2012-10-16 Beckman Coulter, Inc. Flow cytometer sorter
JP5619344B2 (ja) 2007-05-04 2014-11-05 マツクス−プランク−ゲゼルシャフト ツール フエルデルング デル ヴイツセンシャフテン エー フアウ モノリシックマイクロ共振器を使用した光周波数コム発生のための装置と方法
US7982944B2 (en) 2007-05-04 2011-07-19 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method and apparatus for optical frequency comb generation using a monolithic micro-resonator
WO2009005579A1 (en) 2007-06-14 2009-01-08 The General Hospital Corporation High affinity fluorochrome binding peptides
JP5232858B2 (ja) 2007-06-29 2013-07-10 ベクトン・ディキンソン・アンド・カンパニー 生物学的試料の成分を抽出および精製するための方法
WO2009009081A2 (en) 2007-07-10 2009-01-15 Massachusetts Institute Of Technology Tomographic phase microscopy
JP4509163B2 (ja) 2007-10-26 2010-07-21 ソニー株式会社 微小粒子の測定方法
JP4389991B2 (ja) * 2007-10-26 2009-12-24 ソニー株式会社 微小粒子の光学的測定方法及び光学的測定装置
US8159670B2 (en) 2007-11-05 2012-04-17 Abbott Laboratories Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US9451884B2 (en) * 2007-12-13 2016-09-27 Board Of Trustees Of The University Of Arkansas Device and method for in vivo detection of clots within circulatory vessels
GB0800333D0 (en) 2008-01-09 2008-02-20 Ucl Business Plc Beam deflection apparatus and methods
US20090219607A1 (en) 2008-01-17 2009-09-03 Baylor College Of Medicine Method and apparatus for enhanced resolution microscopy of living biological nanostructures
US7920261B2 (en) 2008-02-11 2011-04-05 Massachusetts Institute Of Technology Method and apparatus for detecting and discriminating particles in a fluid
EP2191770B1 (en) 2008-05-02 2016-04-13 Olympus Corporation Optical inspection device, electromagnetic wave detection method, electromagnetic wave detection device, organism observation method, microscope, endoscope, and optical tomographic image generation device
US8140300B2 (en) 2008-05-15 2012-03-20 Becton, Dickinson And Company High throughput flow cytometer operation with data quality assessment and control
US8184279B2 (en) 2008-06-16 2012-05-22 The Regents Of The University Of Colorado, A Body Corporate Fourier domain sensing
CN102150035A (zh) * 2008-09-19 2011-08-10 三井造船株式会社 采用强度调制激光的荧光检测装置和荧光检测方法
US10908084B2 (en) 2008-10-14 2021-02-02 Timothy M. Ragan Devices and methods for direct-sampling analog time-resolved detection
US8440952B2 (en) 2008-11-18 2013-05-14 The Regents Of The University Of California Methods for optical amplified imaging using a two-dimensional spectral brush
US20110275534A1 (en) 2008-12-05 2011-11-10 The Ohio State University MicroRNA-Based Methods and Compositions for the Diagnosis, Prognosis and Treatment of Ovarian Cancer Using a Real-Time PCR Platform
US8233146B2 (en) 2009-01-13 2012-07-31 Becton, Dickinson And Company Cuvette for flow-type particle analyzer
EP2390655A1 (en) * 2009-01-22 2011-11-30 Mitsui Engineering & Shipbuilding Co., Ltd. Fluorescence detecting device and fluorescence detecting method
WO2010084720A1 (ja) 2009-01-23 2010-07-29 三井造船株式会社 蛍光検出装置及び蛍光検出方法
JP4564566B2 (ja) * 2009-02-13 2010-10-20 三井造船株式会社 蛍光検出装置及び蛍光検出方法
JP4620786B2 (ja) 2009-02-17 2011-01-26 三井造船株式会社 蛍光検出方法、蛍光検出装置及びプログラム
JP2012525146A (ja) * 2009-04-28 2012-10-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ 細胞透過のための過剰に荷電されたタンパク質
TWI523720B (zh) * 2009-05-28 2016-03-01 伊雷克托科學工業股份有限公司 應用於雷射處理工件中的特徵的聲光偏轉器及相關雷射處理方法
EP2264168B1 (de) 2009-06-18 2014-12-17 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren
JP5612278B2 (ja) 2009-06-23 2014-10-22 パナソニック株式会社 三次元形状造形物の製造方法およびその製造装置
EP2267430A1 (de) * 2009-06-24 2010-12-29 Masterrind GmbH Vorrichtung und Verfahren zur Selektion von Partikeln
WO2011023593A1 (en) 2009-08-24 2011-03-03 INSERM (Institut National de la Santé et de la Recherche Médicale) Method of and apparatus for imaging a cellular sample
JP5534214B2 (ja) * 2009-10-05 2014-06-25 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
GB0919854D0 (en) * 2009-11-12 2009-12-30 Stfc Science & Technology Detecting species in a dilute medium
JP5649828B2 (ja) 2010-02-03 2015-01-07 オリンパス株式会社 レーザ顕微鏡装置
US9057676B2 (en) 2010-02-05 2015-06-16 Cytonome/St, Llc Multiple flow channel particle analysis system
JP2011163787A (ja) * 2010-02-05 2011-08-25 Sony Corp 微小粒子分析装置及び微小粒子分析方法
JP5551477B2 (ja) 2010-03-15 2014-07-16 オリンパス株式会社 光源装置およびレーザ走査型顕微鏡装置
JP5437148B2 (ja) 2010-04-23 2014-03-12 ベイバイオサイエンス株式会社 フローサイトメータおよびセルソータ
EP2567225B1 (en) 2010-05-04 2019-10-02 Virginia Tech Intellectual Properties, Inc. Lanthionine synthetase component c-like proteins as molecular targets for preventing and treating diseases and disorders
JP2012008055A (ja) 2010-06-25 2012-01-12 Olympus Corp 画像解析方法および画像解析装置
US8384045B2 (en) 2010-07-01 2013-02-26 Sony Corporation Minute particle analyzing device and method
US8574838B2 (en) 2010-07-14 2013-11-05 University Of Medicine And Dentistry Of New Jersey Methods and kits for miRNA isolation and quantitation
US8528427B2 (en) 2010-10-29 2013-09-10 Becton, Dickinson And Company Dual feedback vacuum fluidics for a flow-type particle analyzer
WO2012060163A1 (ja) 2010-11-01 2012-05-10 財団法人神奈川科学技術アカデミー 細胞分析装置
DE102010044013A1 (de) 2010-11-16 2012-05-16 Carl Zeiss Microimaging Gmbh Tiefenauflösungsgesteigerte Mikroskopie
EP3708674B1 (en) 2010-11-16 2023-06-07 1087 Systems, Inc. System for identifying and sorting living cells
US9066657B2 (en) 2010-11-23 2015-06-30 General Electric Company Methods and systems of optical imaging for target detection in a scattering medium
EP2661614B1 (en) 2011-01-03 2025-03-12 Cytonome/ST, LLC Method and apparatus for monitoring and optimizing particle sorting
EP2671065B1 (en) * 2011-02-04 2019-07-10 Cytonome/ST, LLC Particle sorting apparatus and method
JP5930220B2 (ja) 2011-03-18 2016-06-08 国立研究開発法人理化学研究所 非線形光学顕微鏡および非線形光学顕微鏡法
US8990047B2 (en) 2011-03-21 2015-03-24 Becton, Dickinson And Company Neighborhood thresholding in mixed model density gating
JP5620319B2 (ja) 2011-03-23 2014-11-05 オリンパス株式会社 顕微鏡
ES2712636T3 (es) 2011-04-29 2019-05-14 Becton Dickinson Co Sistema y método clasificador de múltiples vías
US8681827B2 (en) 2011-05-16 2014-03-25 Oewaves, Inc. Generation of single optical tone, RF oscillation signal and optical comb in a triple-oscillator device based on nonlinear optical resonator
ES2886583T3 (es) 2011-05-26 2021-12-20 Massachusetts Gen Hospital Sistema de tromboelastografía óptica y método para la evaluación de las métricas de coagulación sanguínea
KR20160103175A (ko) 2011-07-19 2016-08-31 오비지오 이미징 시스템스 엔.브이. 세포 샘플에서의 암 세포들의 검출 및/또는 분류를 위한 방법 및 시스템
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
EP2760413B1 (en) 2011-09-30 2017-12-06 Becton Dickinson and Company Fluid exchange methods and devices
JP5870851B2 (ja) 2012-05-29 2016-03-01 ソニー株式会社 情報処理装置、情報処理方法、及びプログラム
JP2014002470A (ja) 2012-06-15 2014-01-09 Ricoh Co Ltd 処理装置、処理システム、出力方法およびプログラム
US9168568B2 (en) * 2012-08-01 2015-10-27 Owl biomedical, Inc. Particle manipulation system with cytometric confirmation
WO2014031985A1 (en) 2012-08-24 2014-02-27 The Trustees Of Dartmouth College Method and apparatus for magnetic susceptibility tomography, magnetoencephalography, and taggant or contrast agent detection
US8723104B2 (en) 2012-09-13 2014-05-13 City University Of Hong Kong Methods and means for manipulating particles
CN105008895B (zh) 2012-10-15 2019-02-15 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法
JP6338597B2 (ja) * 2013-01-09 2018-06-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 無線周波数多重励起を用いた蛍光イメージングのための装置及び方法
US9862987B2 (en) 2013-01-16 2018-01-09 The Regents Of The University Of California Label free molecular detection methods, systems and devices
US10190963B2 (en) 2013-02-01 2019-01-29 Becton, Dickinson And Company Methods and systems for assessing sample behavior in a flow cytometer
EP2972207B1 (en) 2013-03-14 2022-09-21 Cytonome/ST, LLC Assemblies and methods for reducing optical crosstalk in particle processing systems
WO2014152039A2 (en) 2013-03-14 2014-09-25 Cytonome/St, Llc Operatorless particle processing systems and methods
GB201305317D0 (en) 2013-03-22 2013-05-08 Iles Raymond K Prenatal screening for fetal abnormalities and disorders of pregnancy
WO2014169231A1 (en) 2013-04-12 2014-10-16 Norton Pierce O Automated set-up for cell sorting
US9372143B2 (en) * 2013-05-15 2016-06-21 Captl Llc Scanning image flow cytometer
JP6136843B2 (ja) * 2013-10-17 2017-05-31 ソニー株式会社 粒子分取装置、粒子分取方法及びプログラム
JP6257312B2 (ja) 2013-12-24 2018-01-10 オリンパス株式会社 顕微鏡システム
US9538926B2 (en) 2013-12-26 2017-01-10 Fundacio Institut De Ciencies Fotoniques Speckle contrast optical tomography
CN103762487B (zh) 2014-01-04 2016-05-25 天津奇谱光电技术有限公司 一种具有双输出光束的可调谐激光器
JP6214402B2 (ja) 2014-01-07 2017-10-18 オリンパス株式会社 光刺激装置および顕微鏡システム
US9528925B2 (en) 2014-02-14 2016-12-27 Palo Alto Research Center Incorporated Spatial modulation of light to determine object position
US10451482B2 (en) 2014-02-14 2019-10-22 Palo Alto Research Center Incorporated Determination of color characteristics of objects using spatially modulated light
US9207066B2 (en) 2014-02-14 2015-12-08 Palo Alto Research Center Incorporated Spatial modulation of light to determine dimensional characteristics of objects in a flow path
US20170052106A1 (en) 2014-04-28 2017-02-23 The Broad Institute, Inc. Method for label-free image cytometry
US10267736B2 (en) * 2014-09-30 2019-04-23 The Regents Of The University Of California Imaging flow cytometer using spatial-temporal transformation
JP6652559B2 (ja) 2014-11-12 2020-02-26 オーボテック リミテッド 光学装置及び光学方法
WO2017053592A1 (en) 2015-09-23 2017-03-30 The Regents Of The University Of California Deep learning in label-free cell classification and machine vision extraction of particles
JP6856635B2 (ja) 2015-10-13 2021-04-07 オメガ バイオシステムズ インコーポレイテッド マルチモードの蛍光撮像フローサイトメトリシステム
WO2017066544A1 (en) 2015-10-15 2017-04-20 Woods Hole Oceanographic Institution System for rapid assessment of water quality and harmful algal bloom toxins
JP7033082B2 (ja) 2016-02-05 2022-03-09 ナノビュー バイオサイエンシズ インコーポレイテッド 表面マーカーを有するエキソソームの検出
AU2017234815B2 (en) * 2016-03-17 2022-11-03 Becton, Dickinson And Company Cell sorting using a high throughput fluorescence flow cytometer
WO2017201540A1 (en) 2016-05-20 2017-11-23 Techcyte, Inc. Machine learning classification of particles or substances in digital microscopy images
CN109863384B (zh) 2016-06-10 2022-10-28 加利福尼亚大学董事会 基于图像的细胞分选系统和方法
US10006852B2 (en) 2016-09-13 2018-06-26 Becton, Dickinson And Company Flow cytometer with optical equalization
JP2020537148A (ja) 2017-10-09 2020-12-17 ティーエスアイ インコーポレイテッド 粒子計数器の構成要素の較正
CN112105912B (zh) 2018-04-13 2025-05-23 华盛顿大学 用于单生物纳米颗粒分析的方法和设备
WO2019209723A1 (en) 2018-04-26 2019-10-31 Becton, Dickinson And Company Characterization and sorting for particle analyzers
CN113544490B (zh) 2019-03-29 2024-07-02 贝克顿·迪金森公司 用于颗粒辨别的参数

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110164246A1 (en) * 2008-06-13 2011-07-07 Embl Heidelberg Next generation flow cytometer sorter
WO2015143041A1 (en) * 2014-03-18 2015-09-24 The Regents Of The University Of California Parallel flow cytometer using radiofrequency mulitplexing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HOUSTON, J.P., et al., ‘Digital Analysis and Sorting of Fluorescence Lifetime By Flow Cytometry’, Journal of Quantitative Cell Science Cytometry part A., vol.77A, issue.9 23 August 2010, pp.861-872. *
JENKINS, P. et al., ‘Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres’, Journal of Biophotonics, Wiley, vol.8, iss.11-12, 26 Feb 2015 pp 908-917 *

Also Published As

Publication number Publication date
US20240410813A1 (en) 2024-12-12
US20200209140A1 (en) 2020-07-02
EP3430376B8 (en) 2024-11-27
US10324019B2 (en) 2019-06-18
JP7130562B2 (ja) 2022-09-05
CN109564151B (zh) 2023-01-24
US11774343B2 (en) 2023-10-03
US20190234863A1 (en) 2019-08-01
KR20180129832A (ko) 2018-12-05
EP3430376B1 (en) 2024-10-23
AU2017234815A1 (en) 2018-10-04
US11105728B2 (en) 2021-08-31
JP2022095825A (ja) 2022-06-28
EP4545936A2 (en) 2025-04-30
KR102641469B1 (ko) 2024-02-28
KR20220002706A (ko) 2022-01-06
BR112018068866B1 (pt) 2022-11-29
US10620111B2 (en) 2020-04-14
JP2024119909A (ja) 2024-09-03
US20230076378A1 (en) 2023-03-09
US12078587B2 (en) 2024-09-03
CA3018065A1 (en) 2017-09-21
US20170268981A1 (en) 2017-09-21
JP7502361B2 (ja) 2024-06-18
KR102527830B1 (ko) 2023-05-02
EP4545936A3 (en) 2025-07-16
KR20230003444A (ko) 2023-01-05
CN109564151A (zh) 2019-04-02
US20250155356A1 (en) 2025-05-15
JP2019513236A (ja) 2019-05-23
US20210356381A1 (en) 2021-11-18
WO2017161247A1 (en) 2017-09-21
EP3430376A1 (en) 2019-01-23
US12313521B1 (en) 2025-05-27
ZA201806386B (en) 2020-02-26
BR112018068866A2 (pt) 2019-01-22

Similar Documents

Publication Publication Date Title
US12313521B1 (en) Cell sorting using a high throughput fluorescence flow cytometer
US12038372B2 (en) Multi-modal fluorescence imaging flow cytometry system
US12436087B2 (en) Spectral unmixing of fluorescence imaging using radiofrequency-multiplexed excitation data

Legal Events

Date Code Title Description
PC1 Assignment before grant (sect. 113)

Owner name: BECTON, DICKINSON AND COMPANY

Free format text: FORMER APPLICANT(S): BD BIOSCIENCES

FGA Letters patent sealed or granted (standard patent)