KR102406976B1 - 재조합 단백질의 효율적인 선택성 - Google Patents

재조합 단백질의 효율적인 선택성 Download PDF

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KR102406976B1
KR102406976B1 KR1020177006620A KR20177006620A KR102406976B1 KR 102406976 B1 KR102406976 B1 KR 102406976B1 KR 1020177006620 A KR1020177006620 A KR 1020177006620A KR 20177006620 A KR20177006620 A KR 20177006620A KR 102406976 B1 KR102406976 B1 KR 102406976B1
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leu
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phe
cell
ile
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KR20170055481A (ko
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디팔리 데슈판데
다리아 부라코프
강 첸
제임스 판들
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리제너론 파마슈티칼스 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020177006620A 2014-08-19 2015-08-19 재조합 단백질의 효율적인 선택성 Active KR102406976B1 (ko)

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KR1020227018877A KR102556383B1 (ko) 2014-08-19 2015-08-19 재조합 단백질의 효율적인 선택성

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US201462039416P 2014-08-19 2014-08-19
US62/039,416 2014-08-19
PCT/US2015/045793 WO2016028838A1 (en) 2014-08-19 2015-08-19 Efficient selectivity of recombinant proteins

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KR20170055481A KR20170055481A (ko) 2017-05-19
KR102406976B1 true KR102406976B1 (ko) 2022-06-10

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KR1020227018877A Active KR102556383B1 (ko) 2014-08-19 2015-08-19 재조합 단백질의 효율적인 선택성

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US (4) US9732357B2 (https=)
EP (2) EP3183359B1 (https=)
JP (4) JP7115851B2 (https=)
KR (2) KR102406976B1 (https=)
CN (2) CN113293139B (https=)
AR (1) AR102333A1 (https=)
AU (3) AU2015305565B9 (https=)
CA (1) CA2957036A1 (https=)
CY (1) CY1124079T1 (https=)
DK (1) DK3183359T3 (https=)
EA (1) EA035340B1 (https=)
ES (1) ES2849728T3 (https=)
HR (1) HRP20210301T1 (https=)
HU (1) HUE054069T2 (https=)
IL (3) IL287020B (https=)
LT (1) LT3183359T (https=)
MA (1) MA40371B1 (https=)
MX (2) MX384990B (https=)
MY (1) MY191745A (https=)
PL (1) PL3183359T3 (https=)
PT (1) PT3183359T (https=)
RS (1) RS61371B1 (https=)
SG (2) SG11201701121UA (https=)
SI (1) SI3183359T1 (https=)
SM (1) SMT202100054T1 (https=)
TW (3) TWI777374B (https=)
WO (1) WO2016028838A1 (https=)

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Publication number Priority date Publication date Assignee Title
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
CN111712566A (zh) * 2018-02-08 2020-09-25 应用干细胞有限公司 用于筛选靶基因变体的方法
CN117623782A (zh) 2019-10-23 2024-03-01 佳能株式会社 制造陶瓷制品的方法和陶瓷制品

Citations (1)

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JP2005027599A (ja) 2003-07-09 2005-02-03 Nara Institute Of Science & Technology 植物由来遺伝子を用いた遺伝子導入植物の選抜法

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US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US6077835A (en) 1994-03-23 2000-06-20 Case Western Reserve University Delivery of compacted nucleic acid to cells
KR970029803A (ko) 1995-11-03 1997-06-26 김광호 반도체 메모리장치의 프리차지 회로
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
US6281005B1 (en) 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
CA2402563A1 (en) * 1999-12-23 2001-07-26 Hyseq, Inc. Novel nucleic acids and polypeptides
US6919183B2 (en) 2001-01-16 2005-07-19 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins
JP4554370B2 (ja) 2002-03-29 2010-09-29 ゾーマ テクノロジー リミテッド 多重遺伝子プラスミドおよび組み換えポリペプチドの発現増加法
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US7384744B2 (en) 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
EP1586585A1 (de) * 2004-04-14 2005-10-19 F. Hoffmann-La Roche Ag Expressionssystem zur Herstellung von IL-15/Fc-Fusionsproteinen und ihre Verwendung
CN106987561A (zh) 2006-12-07 2017-07-28 陶氏益农公司 新型选择性标记基因
EP2150617B1 (en) 2007-06-04 2014-10-22 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
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JP2005027599A (ja) 2003-07-09 2005-02-03 Nara Institute Of Science & Technology 植物由来遺伝子を用いた遺伝子導入植物の選抜法

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KR102556383B1 (ko) 2023-07-17
MX2021009154A (es) 2021-09-10
AU2020205336B2 (en) 2022-11-10
AU2015305565B9 (en) 2020-05-21
TWI719945B (zh) 2021-03-01
IL287020A (en) 2021-12-01
EA201790241A1 (ru) 2017-06-30
US20170335341A1 (en) 2017-11-23
US9732357B2 (en) 2017-08-15
IL295361B1 (en) 2023-04-01
JP2020174681A (ja) 2020-10-29
BR112017003074A2 (pt) 2018-03-06
AU2023200752B2 (en) 2024-02-29
CA2957036A1 (en) 2016-02-25
AU2015305565B2 (en) 2020-04-30
IL250531A0 (en) 2017-03-30
HRP20210301T1 (hr) 2021-04-02
SG11201701121UA (en) 2017-03-30
CY1124079T1 (el) 2022-05-27
PL3183359T3 (pl) 2021-08-02
TW201615833A (zh) 2016-05-01
LT3183359T (lt) 2021-02-25
MY191745A (en) 2022-07-13
SG10201707859RA (en) 2017-10-30
CN107075549A (zh) 2017-08-18
MX384990B (es) 2025-03-14
TW202124716A (zh) 2021-07-01
PT3183359T (pt) 2021-02-03
IL250531B (en) 2021-10-31
SMT202100054T1 (it) 2021-03-15
IL295361A (en) 2022-10-01
AU2023200752A1 (en) 2023-03-09
MX2017002203A (es) 2017-05-22
US20160053280A1 (en) 2016-02-25
RS61371B1 (sr) 2021-02-26
MA40371A (fr) 2017-06-28
HUE054069T2 (hu) 2021-08-30
TWI777374B (zh) 2022-09-11
US20210340564A1 (en) 2021-11-04
CN107075549B (zh) 2021-04-20
AU2020205336A1 (en) 2020-08-06
TWI832364B (zh) 2024-02-11
ES2849728T3 (es) 2021-08-20
CN113293139B (zh) 2025-04-29
US20200063159A1 (en) 2020-02-27
MA40371B1 (fr) 2021-03-31
EA035340B1 (ru) 2020-05-29
JP7115851B2 (ja) 2022-08-09
DK3183359T3 (da) 2021-02-01
EP3901274A1 (en) 2021-10-27
WO2016028838A1 (en) 2016-02-25
IL295361B2 (en) 2023-08-01
JP7260510B2 (ja) 2023-04-18
TW202246506A (zh) 2022-12-01
AR102333A1 (es) 2017-02-22
JP2023076667A (ja) 2023-06-01
EP3183359B1 (en) 2020-12-16
US10457959B2 (en) 2019-10-29
IL287020B (en) 2022-09-01
JP7561232B2 (ja) 2024-10-03
JP2024166420A (ja) 2024-11-28
JP2017525368A (ja) 2017-09-07
EP3183359A1 (en) 2017-06-28
KR20220080215A (ko) 2022-06-14
EP3183359A4 (en) 2018-04-04
AU2015305565A1 (en) 2017-03-09
CN113293139A (zh) 2021-08-24
US11085053B2 (en) 2021-08-10
SI3183359T1 (sl) 2021-03-31
KR20170055481A (ko) 2017-05-19

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