MX2017002203A - Selectividad eficaz de proteinas recombinantes. - Google Patents

Selectividad eficaz de proteinas recombinantes.

Info

Publication number
MX2017002203A
MX2017002203A MX2017002203A MX2017002203A MX2017002203A MX 2017002203 A MX2017002203 A MX 2017002203A MX 2017002203 A MX2017002203 A MX 2017002203A MX 2017002203 A MX2017002203 A MX 2017002203A MX 2017002203 A MX2017002203 A MX 2017002203A
Authority
MX
Mexico
Prior art keywords
expression
recombinant proteins
mammalian
invention includes
gpt
Prior art date
Application number
MX2017002203A
Other languages
English (en)
Inventor
Chen Gang
Burakov Darya
Deshpande Dipali
Fandl James
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2017002203A publication Critical patent/MX2017002203A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La invención proporciona un nuevo sistema de expresión que comprende un marcador de selección de mamífero que promueve modificaciones postraduccionales convenientes de las glicoproteínas. En particular, la invención incluye métodos y composiciones para la expresión óptima de proteínas recombinantes en células de mamíferos mediante el empleo de un sistema con marcador de selección basado en genes de GPT de origen mamífero. La invención incluye métodos que facilitan la selectividad y aumentan las copias de expresión así como el rendimiento de proteínas recombinantes en células de mamíferos, y métodos para el uso de los sistemas de expresión de GPT.
MX2017002203A 2014-08-19 2015-08-19 Selectividad eficaz de proteinas recombinantes. MX2017002203A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462039416P 2014-08-19 2014-08-19
PCT/US2015/045793 WO2016028838A1 (en) 2014-08-19 2015-08-19 Efficient selectivity of recombinant proteins

Publications (1)

Publication Number Publication Date
MX2017002203A true MX2017002203A (es) 2017-05-22

Family

ID=55347784

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017002203A MX2017002203A (es) 2014-08-19 2015-08-19 Selectividad eficaz de proteinas recombinantes.
MX2021009154A MX2021009154A (es) 2014-08-19 2017-02-17 Selectividad eficaz de proteinas recombinantes.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2021009154A MX2021009154A (es) 2014-08-19 2017-02-17 Selectividad eficaz de proteinas recombinantes.

Country Status (27)

Country Link
US (4) US9732357B2 (es)
EP (2) EP3183359B1 (es)
JP (3) JP7115851B2 (es)
KR (2) KR102406976B1 (es)
CN (2) CN107075549B (es)
AR (1) AR102333A1 (es)
AU (3) AU2015305565B9 (es)
BR (1) BR112017003074A2 (es)
CA (1) CA2957036A1 (es)
CY (1) CY1124079T1 (es)
DK (1) DK3183359T3 (es)
EA (1) EA035340B1 (es)
ES (1) ES2849728T3 (es)
HR (1) HRP20210301T1 (es)
HU (1) HUE054069T2 (es)
IL (3) IL287020B (es)
LT (1) LT3183359T (es)
MA (1) MA40371B1 (es)
MX (2) MX2017002203A (es)
MY (1) MY191745A (es)
PL (1) PL3183359T3 (es)
PT (1) PT3183359T (es)
RS (1) RS61371B1 (es)
SG (2) SG10201707859RA (es)
SI (1) SI3183359T1 (es)
TW (3) TWI719945B (es)
WO (1) WO2016028838A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
CN111712566A (zh) * 2018-02-08 2020-09-25 应用干细胞有限公司 用于筛选靶基因变体的方法
WO2021079909A1 (ja) 2019-10-23 2021-04-29 キヤノン株式会社 セラミックス物品の製造方法およびセラミックス物品

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US6077835A (en) 1994-03-23 2000-06-20 Case Western Reserve University Delivery of compacted nucleic acid to cells
KR970029803A (ko) 1995-11-03 1997-06-26 김광호 반도체 메모리장치의 프리차지 회로
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
US6281005B1 (en) 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
EP1392859B1 (en) 2001-01-16 2006-05-10 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins
EP1492874B1 (en) 2002-03-29 2011-01-19 XOMA Technology Ltd. Multigenic vector plasmids and methods for increasing expression of recombinant polypeptides
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US7384744B2 (en) * 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
JP2005027599A (ja) * 2003-07-09 2005-02-03 Nara Institute Of Science & Technology 植物由来遺伝子を用いた遺伝子導入植物の選抜法
EP1586585A1 (de) * 2004-04-14 2005-10-19 F. Hoffmann-La Roche Ag Expressionssystem zur Herstellung von IL-15/Fc-Fusionsproteinen und ihre Verwendung
PL2610334T3 (pl) 2006-12-07 2017-05-31 Dow Agrosciences Llc Nowe geny markerów selekcyjnych
PL2150617T3 (pl) 2007-06-04 2015-04-30 Regeneron Pharma Wzmocniona ekspresja i regiony stabilności
KR100927803B1 (ko) * 2007-11-23 2009-11-23 한국생명공학연구원 식물 형질전환체를 선별하기 위한 마커로서 ｇｐｔ 유전자
PT2796466T (pt) * 2007-12-07 2018-02-23 Zymogenetics Inc Moléculas de anticorpo humanizado específicas para il-31
CA2722423A1 (en) * 2008-04-22 2009-10-29 Rutgers, The State University Hcv e2 construct compositions and methods
CN102307993B (zh) * 2008-12-09 2014-06-25 哈洛齐梅公司 延长的可溶性ph20多肽及其用途
EP2674495A1 (en) 2012-06-14 2013-12-18 Sanofi CHO expression system
AR102333A1 (es) * 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas

Also Published As

Publication number Publication date
JP2023076667A (ja) 2023-06-01
KR20220080215A (ko) 2022-06-14
IL295361A (en) 2022-10-01
US9732357B2 (en) 2017-08-15
TW201615833A (zh) 2016-05-01
LT3183359T (lt) 2021-02-25
HRP20210301T1 (hr) 2021-04-02
JP2020174681A (ja) 2020-10-29
EP3183359A1 (en) 2017-06-28
IL287020A (en) 2021-12-01
AU2015305565B9 (en) 2020-05-21
PL3183359T3 (pl) 2021-08-02
RS61371B1 (sr) 2021-02-26
EP3183359A4 (en) 2018-04-04
MA40371A (fr) 2017-06-28
WO2016028838A1 (en) 2016-02-25
TWI832364B (zh) 2024-02-11
EA201790241A1 (ru) 2017-06-30
CN107075549B (zh) 2021-04-20
JP7115851B2 (ja) 2022-08-09
MX2021009154A (es) 2021-09-10
EA035340B1 (ru) 2020-05-29
BR112017003074A2 (pt) 2018-03-06
SG10201707859RA (en) 2017-10-30
KR102556383B1 (ko) 2023-07-17
MA40371B1 (fr) 2021-03-31
EP3901274A1 (en) 2021-10-27
US20160053280A1 (en) 2016-02-25
IL287020B (en) 2022-09-01
TWI719945B (zh) 2021-03-01
CN113293139A (zh) 2021-08-24
AU2023200752A1 (en) 2023-03-09
IL250531A0 (en) 2017-03-30
IL250531B (en) 2021-10-31
TW202124716A (zh) 2021-07-01
ES2849728T3 (es) 2021-08-20
JP7260510B2 (ja) 2023-04-18
US20210340564A1 (en) 2021-11-04
DK3183359T3 (da) 2021-02-01
AU2020205336B2 (en) 2022-11-10
MY191745A (en) 2022-07-13
TWI777374B (zh) 2022-09-11
EP3183359B1 (en) 2020-12-16
SI3183359T1 (sl) 2021-03-31
US10457959B2 (en) 2019-10-29
IL295361B1 (en) 2023-04-01
JP2017525368A (ja) 2017-09-07
AR102333A1 (es) 2017-02-22
CN107075549A (zh) 2017-08-18
KR20170055481A (ko) 2017-05-19
KR102406976B1 (ko) 2022-06-10
AU2023200752B2 (en) 2024-02-29
CA2957036A1 (en) 2016-02-25
AU2015305565B2 (en) 2020-04-30
SG11201701121UA (en) 2017-03-30
US11085053B2 (en) 2021-08-10
US20200063159A1 (en) 2020-02-27
TW202246506A (zh) 2022-12-01
PT3183359T (pt) 2021-02-03
AU2020205336A1 (en) 2020-08-06
HUE054069T2 (hu) 2021-08-30
CY1124079T1 (el) 2022-05-27
AU2015305565A1 (en) 2017-03-09
IL295361B2 (en) 2023-08-01
US20170335341A1 (en) 2017-11-23

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