EA201790241A1 - Эффективная селективность в отношении рекомбинантных белков - Google Patents

Эффективная селективность в отношении рекомбинантных белков

Info

Publication number
EA201790241A1
EA201790241A1 EA201790241A EA201790241A EA201790241A1 EA 201790241 A1 EA201790241 A1 EA 201790241A1 EA 201790241 A EA201790241 A EA 201790241A EA 201790241 A EA201790241 A EA 201790241A EA 201790241 A1 EA201790241 A1 EA 201790241A1
Authority
EA
Eurasian Patent Office
Prior art keywords
recombinant proteins
present
provides
expression
methods
Prior art date
Application number
EA201790241A
Other languages
English (en)
Other versions
EA035340B1 (ru
Inventor
Дипали Дэшпанде
Дарья Бураков
Ган Чэнь
Джэймс Фандл
Original Assignee
Регенерон Фармасьютикалз, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Регенерон Фармасьютикалз, Инк. filed Critical Регенерон Фармасьютикалз, Инк.
Publication of EA201790241A1 publication Critical patent/EA201790241A1/ru
Publication of EA035340B1 publication Critical patent/EA035340B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Настоящее изобретение предусматривает новую систему экспрессии, включающую селективный маркер млекопитающего, которая обеспечивает требуемые посттрансляционные модификации гликопротеинов. В частности, настоящее изобретение предусматривает способы и композиции для оптимальной экспрессии рекомбинантного белка в клетках млекопитающих с применением системы селективных маркеров на основе генов GPT, происходящих из млекопитающих. Настоящее изобретение включает способы, которые обеспечивают селективность и усиливают экспрессию копий, а также выход рекомбинантных белков в клетках млекопитающих, и способы применения систем экспрессии GPT.
EA201790241A 2014-08-19 2015-08-19 Эффективная селективность в отношении рекомбинантных белков EA035340B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462039416P 2014-08-19 2014-08-19
PCT/US2015/045793 WO2016028838A1 (en) 2014-08-19 2015-08-19 Efficient selectivity of recombinant proteins

Publications (2)

Publication Number Publication Date
EA201790241A1 true EA201790241A1 (ru) 2017-06-30
EA035340B1 EA035340B1 (ru) 2020-05-29

Family

ID=55347784

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201790241A EA035340B1 (ru) 2014-08-19 2015-08-19 Эффективная селективность в отношении рекомбинантных белков

Country Status (27)

Country Link
US (4) US9732357B2 (ru)
EP (2) EP3183359B1 (ru)
JP (3) JP7115851B2 (ru)
KR (2) KR102406976B1 (ru)
CN (2) CN113293139A (ru)
AR (1) AR102333A1 (ru)
AU (3) AU2015305565B9 (ru)
BR (1) BR112017003074A2 (ru)
CA (1) CA2957036A1 (ru)
CY (1) CY1124079T1 (ru)
DK (1) DK3183359T3 (ru)
EA (1) EA035340B1 (ru)
ES (1) ES2849728T3 (ru)
HR (1) HRP20210301T1 (ru)
HU (1) HUE054069T2 (ru)
IL (3) IL287020B (ru)
LT (1) LT3183359T (ru)
MA (1) MA40371B1 (ru)
MX (2) MX2017002203A (ru)
MY (1) MY191745A (ru)
PL (1) PL3183359T3 (ru)
PT (1) PT3183359T (ru)
RS (1) RS61371B1 (ru)
SG (2) SG11201701121UA (ru)
SI (1) SI3183359T1 (ru)
TW (3) TWI777374B (ru)
WO (1) WO2016028838A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102333A1 (es) * 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
EP3749754A4 (en) * 2018-02-08 2021-10-20 Applied Stemcell, Inc. PROCEDURE FOR SCREENING VARIANTS OF A TARGET
EP4049989A4 (en) 2019-10-23 2023-11-29 Canon Kabushiki Kaisha METHOD FOR PRODUCING A CERAMIC ARTICLE, AND CERAMIC ARTICLE

Family Cites Families (21)

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US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US6077835A (en) 1994-03-23 2000-06-20 Case Western Reserve University Delivery of compacted nucleic acid to cells
KR970029803A (ko) 1995-11-03 1997-06-26 김광호 반도체 메모리장치의 프리차지 회로
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
US6281005B1 (en) 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
EP1392859B1 (en) 2001-01-16 2006-05-10 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins
US7192737B2 (en) 2002-03-29 2007-03-20 Xoma Technology Ltd. Methods and materials for increasing expression of recombinant polypeptides
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US7384744B2 (en) 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
JP2005027599A (ja) * 2003-07-09 2005-02-03 Nara Institute Of Science & Technology 植物由来遺伝子を用いた遺伝子導入植物の選抜法
EP1586585A1 (de) * 2004-04-14 2005-10-19 F. Hoffmann-La Roche Ag Expressionssystem zur Herstellung von IL-15/Fc-Fusionsproteinen und ihre Verwendung
HUE026199T2 (en) 2006-12-07 2016-05-30 Dow Agrosciences Llc New selectable marker genes
ES2522615T3 (es) 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas
KR100927803B1 (ko) * 2007-11-23 2009-11-23 한국생명공학연구원 식물 형질전환체를 선별하기 위한 마커로서 ｇｐｔ 유전자
ES2660036T3 (es) * 2007-12-07 2018-03-20 Zymogenetics, Inc. Moléculas de anticuerpo humanizadas específicas para IL-31
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AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas

Also Published As

Publication number Publication date
JP2017525368A (ja) 2017-09-07
SI3183359T1 (sl) 2021-03-31
LT3183359T (lt) 2021-02-25
TW201615833A (zh) 2016-05-01
TW202124716A (zh) 2021-07-01
JP2020174681A (ja) 2020-10-29
BR112017003074A2 (pt) 2018-03-06
AU2023200752A1 (en) 2023-03-09
WO2016028838A1 (en) 2016-02-25
CN107075549A (zh) 2017-08-18
AU2015305565A1 (en) 2017-03-09
KR102556383B1 (ko) 2023-07-17
AU2015305565B2 (en) 2020-04-30
IL295361A (en) 2022-10-01
IL287020B (en) 2022-09-01
IL250531A0 (en) 2017-03-30
EA035340B1 (ru) 2020-05-29
DK3183359T3 (da) 2021-02-01
AU2015305565B9 (en) 2020-05-21
KR20170055481A (ko) 2017-05-19
RS61371B1 (sr) 2021-02-26
EP3183359B1 (en) 2020-12-16
CN107075549B (zh) 2021-04-20
EP3183359A4 (en) 2018-04-04
JP7260510B2 (ja) 2023-04-18
US20210340564A1 (en) 2021-11-04
US11085053B2 (en) 2021-08-10
US9732357B2 (en) 2017-08-15
HUE054069T2 (hu) 2021-08-30
AU2020205336B2 (en) 2022-11-10
TWI832364B (zh) 2024-02-11
US20160053280A1 (en) 2016-02-25
US20200063159A1 (en) 2020-02-27
KR102406976B1 (ko) 2022-06-10
AU2023200752B2 (en) 2024-02-29
TW202246506A (zh) 2022-12-01
EP3183359A1 (en) 2017-06-28
MA40371B1 (fr) 2021-03-31
JP7115851B2 (ja) 2022-08-09
PT3183359T (pt) 2021-02-03
IL287020A (en) 2021-12-01
AU2020205336A1 (en) 2020-08-06
CN113293139A (zh) 2021-08-24
EP3901274A1 (en) 2021-10-27
SG11201701121UA (en) 2017-03-30
CY1124079T1 (el) 2022-05-27
SG10201707859RA (en) 2017-10-30
US20170335341A1 (en) 2017-11-23
MY191745A (en) 2022-07-13
HRP20210301T1 (hr) 2021-04-02
IL295361B2 (en) 2023-08-01
ES2849728T3 (es) 2021-08-20
PL3183359T3 (pl) 2021-08-02
IL295361B1 (en) 2023-04-01
AR102333A1 (es) 2017-02-22
IL250531B (en) 2021-10-31
JP2023076667A (ja) 2023-06-01
TWI719945B (zh) 2021-03-01
MA40371A (fr) 2017-06-28
US10457959B2 (en) 2019-10-29
MX2017002203A (es) 2017-05-22
TWI777374B (zh) 2022-09-11
KR20220080215A (ko) 2022-06-14
MX2021009154A (es) 2021-09-10
CA2957036A1 (en) 2016-02-25

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