KR102202742B1 - 광열 플랫폼을 이용한 살아있는 세포 내로의 고속대량 주입물 전달 - Google Patents
광열 플랫폼을 이용한 살아있는 세포 내로의 고속대량 주입물 전달 Download PDFInfo
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- KR102202742B1 KR102202742B1 KR1020157027342A KR20157027342A KR102202742B1 KR 102202742 B1 KR102202742 B1 KR 102202742B1 KR 1020157027342 A KR1020157027342 A KR 1020157027342A KR 20157027342 A KR20157027342 A KR 20157027342A KR 102202742 B1 KR102202742 B1 KR 102202742B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
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- Electromagnetism (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361799222P | 2013-03-15 | 2013-03-15 | |
| US61/799,222 | 2013-03-15 | ||
| PCT/US2014/026618 WO2014151888A1 (en) | 2013-03-15 | 2014-03-13 | High-throughput cargo delivery into live cells using photothermal platforms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20150130351A KR20150130351A (ko) | 2015-11-23 |
| KR102202742B1 true KR102202742B1 (ko) | 2021-01-12 |
Family
ID=51581064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020157027342A Active KR102202742B1 (ko) | 2013-03-15 | 2014-03-13 | 광열 플랫폼을 이용한 살아있는 세포 내로의 고속대량 주입물 전달 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10982217B2 (enExample) |
| EP (1) | EP2971011B1 (enExample) |
| JP (1) | JP6606058B2 (enExample) |
| KR (1) | KR102202742B1 (enExample) |
| CN (1) | CN105452471A (enExample) |
| AU (1) | AU2014236747B2 (enExample) |
| CA (1) | CA2905999A1 (enExample) |
| IL (1) | IL241150B (enExample) |
| WO (1) | WO2014151888A1 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2873204C (en) | 2011-05-13 | 2021-10-19 | The Regents Of The University Of California | Photothermal substrates for selective transfection of cells |
| ES2764105T3 (es) | 2011-10-17 | 2020-06-02 | Massachusetts Inst Technology | Administración intracelular |
| US11967839B2 (en) | 2011-12-30 | 2024-04-23 | Analog Devices, Inc. | Electromagnetic connector for an industrial control system |
| US10834094B2 (en) | 2013-08-06 | 2020-11-10 | Bedrock Automation Platforms Inc. | Operator action authentication in an industrial control system |
| US8862802B2 (en) | 2011-12-30 | 2014-10-14 | Bedrock Automation Platforms Inc. | Switch fabric having a serial communications interface and a parallel communications interface |
| US11144630B2 (en) | 2011-12-30 | 2021-10-12 | Bedrock Automation Platforms Inc. | Image capture devices for a secure industrial control system |
| US9467297B2 (en) | 2013-08-06 | 2016-10-11 | Bedrock Automation Platforms Inc. | Industrial control system redundant communications/control modules authentication |
| US9449756B2 (en) * | 2013-05-02 | 2016-09-20 | Bedrock Automation Platforms Inc. | Electromagnetic connectors |
| US12061685B2 (en) | 2011-12-30 | 2024-08-13 | Analog Devices, Inc. | Image capture devices for a secure industrial control system |
| US8868813B2 (en) | 2011-12-30 | 2014-10-21 | Bedrock Automation Platforms Inc. | Communications control system with a serial communications interface and a parallel communications interface |
| US11314854B2 (en) | 2011-12-30 | 2022-04-26 | Bedrock Automation Platforms Inc. | Image capture devices for a secure industrial control system |
| US10834820B2 (en) | 2013-08-06 | 2020-11-10 | Bedrock Automation Platforms Inc. | Industrial control system cable |
| US9600434B1 (en) | 2011-12-30 | 2017-03-21 | Bedrock Automation Platforms, Inc. | Switch fabric having a serial communications interface and a parallel communications interface |
| US9437967B2 (en) | 2011-12-30 | 2016-09-06 | Bedrock Automation Platforms, Inc. | Electromagnetic connector for an industrial control system |
| US8971072B2 (en) | 2011-12-30 | 2015-03-03 | Bedrock Automation Platforms Inc. | Electromagnetic connector for an industrial control system |
| US9727511B2 (en) | 2011-12-30 | 2017-08-08 | Bedrock Automation Platforms Inc. | Input/output module with multi-channel switching capability |
| US9191203B2 (en) | 2013-08-06 | 2015-11-17 | Bedrock Automation Platforms Inc. | Secure industrial control system |
| JP6606058B2 (ja) | 2013-03-15 | 2019-11-13 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 光熱プラットフォームを使用する生細胞への高スループットカーゴ送達 |
| US10613567B2 (en) | 2013-08-06 | 2020-04-07 | Bedrock Automation Platforms Inc. | Secure power supply for an industrial control system |
| KR102304167B1 (ko) | 2013-08-16 | 2021-09-24 | 메사추세츠 인스티튜트 오브 테크놀로지 | 세포로의 선택적 물질 전달 |
| WO2015148842A1 (en) | 2014-03-28 | 2015-10-01 | The Regents Of The University Of California | Efficient delivery of large cargos into cells on a porous substrate |
| CN111293495B (zh) | 2014-07-07 | 2022-05-24 | 基岩自动化平台公司 | 工业控制系统电缆 |
| US11111472B2 (en) | 2014-10-31 | 2021-09-07 | Massachusetts Institute Of Technology | Delivery of biomolecules to immune cells |
| CN113897285A (zh) | 2014-11-14 | 2022-01-07 | 麻省理工学院 | 化合物和组合物向细胞中的破坏和场实现的递送 |
| US11125739B2 (en) | 2015-01-12 | 2021-09-21 | Massachusetts Institute Of Technology | Gene editing through microfluidic delivery |
| JP6090891B1 (ja) | 2015-06-01 | 2017-03-08 | 株式会社片岡製作所 | 細胞処理方法、レーザ加工機、細胞培養容器 |
| EP4257675A3 (en) | 2015-07-09 | 2024-01-03 | Massachusetts Institute of Technology | Delivery of materials to anucleate cells |
| CN105420278A (zh) * | 2015-12-09 | 2016-03-23 | 苏州大学 | 采用光致穿孔的方式制备负载有外源分子细胞的方法及制备该细胞用的基材及该细胞 |
| CN105670620B (zh) * | 2016-03-14 | 2017-11-14 | 山东农业大学 | 一种掺杂氮化碳荧光量子点的制备方法 |
| US20190264229A1 (en) * | 2016-10-21 | 2019-08-29 | Agency For Science, Technology And Research | Device, system and method for intracellular delivery of substances |
| WO2019157316A2 (en) * | 2018-02-12 | 2019-08-15 | Flagship Pioneering Innovations V, Inc. | Methods and devices for the isolation of subcellular components |
| CN109142293A (zh) * | 2018-07-27 | 2019-01-04 | 福州大学 | 一种基于氮化碳解吸附作用的荧光传感器及其制备和应用 |
| KR102108023B1 (ko) * | 2018-09-28 | 2020-05-07 | 포항공과대학교 산학협력단 | 이작용성 나노자임 및 이의 용도 |
| US12496274B2 (en) | 2018-11-14 | 2025-12-16 | Flagship Pioneering Innovations V, Inc. | Compositions and methods for compartment-specific cargo delivery |
| CN110261365B (zh) * | 2019-07-02 | 2021-04-02 | 吉林大学 | 一种周期月牙形纳米间隙阵列及其制备方法 |
| US10895727B1 (en) * | 2019-10-19 | 2021-01-19 | SequLITE Genomics US, Inc. | Microscope for locating structures on the inner surface of a fluidic channel |
| AU2020366521B2 (en) * | 2019-10-19 | 2024-03-07 | SequLITE Genomics US, Inc. | Virtual fiducials |
| WO2021109132A1 (zh) * | 2019-12-06 | 2021-06-10 | 百脉迪生物科技(苏州)有限公司 | 一种复合材料及其制备方法和应用 |
| WO2021178477A1 (en) * | 2020-03-03 | 2021-09-10 | Arizona Board Of Regents On Behalf Of Arizona State University | Low-capacitance nanopore sensors on insulating substrates |
| WO2021251979A1 (en) * | 2020-06-12 | 2021-12-16 | Hewlett-Packard Development Company, L.P. | Microfluidic passage with protective layer |
| WO2022031700A1 (en) * | 2020-08-03 | 2022-02-10 | The Regents Of The University Of California | Laser-actuated supercritical injector |
| CN112461807B (zh) * | 2020-11-26 | 2021-11-19 | 山西大学 | 碳量子点在靶向细胞核仁免洗成像中的应用 |
| CN115888790B (zh) * | 2022-11-14 | 2024-10-15 | 南京先进生物材料与过程装备研究院有限公司 | 一种利用微通道制备氮化碳纳米片的方法 |
| WO2024233806A2 (en) * | 2023-05-09 | 2024-11-14 | Met Biotechnology, Inc. | Methods of engineering cells with mutated genomes using intact large cargo transfer systems |
| WO2025117977A1 (en) * | 2023-11-30 | 2025-06-05 | Met Biotechnology, Inc. | Electroporation systems and methods for cargo loading into extracellular vesicles |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009017695A1 (en) | 2007-07-26 | 2009-02-05 | The Regents Of The University Of California | A single cell surgery tool and a cell transfection device utilizing the photothermal properties of thin films and/or metal nanoparticles |
| WO2012158631A2 (en) * | 2011-05-13 | 2012-11-22 | The Regents Of The University Of California | Photothermal substrates for selective transfection of cells |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310674A (en) | 1982-05-10 | 1994-05-10 | Bar-Ilan University | Apertured cell carrier |
| JPH01141582A (ja) | 1987-11-27 | 1989-06-02 | Shimadzu Corp | 遺伝子導入装置 |
| US4917462A (en) | 1988-06-15 | 1990-04-17 | Cornell Research Foundation, Inc. | Near field scanning optical microscopy |
| US5080586A (en) | 1990-09-24 | 1992-01-14 | Osada Research Institute, Ltd. | Apical foramen position detector for use in dental treatment |
| JP2783984B2 (ja) | 1995-04-21 | 1998-08-06 | プリマハム株式会社 | マイクロマニピュレーション装置及びそれを用いた細胞操作方法 |
| US5922687A (en) | 1995-05-04 | 1999-07-13 | Board Of Trustees Of The Leland Stanford Junior University | Intracellular delivery of nucleic acids using pressure |
| US6052286A (en) * | 1997-04-11 | 2000-04-18 | Texas Instruments Incorporated | Restrained center core anisotropically conductive adhesive |
| WO1999044638A1 (en) | 1998-03-06 | 1999-09-10 | Spectrx, Inc. | Photothermal structure for biomedical applications, and method therefor |
| US6537800B1 (en) | 1998-03-12 | 2003-03-25 | Center For Advanced Science And Technology Incubation, Ltd. | Apparatus for automatically measuring minute membrane potential |
| AU3277199A (en) | 1998-03-12 | 1999-09-27 | Isao Karube | Techniques for piercing specific site of cell |
| JP3035608B2 (ja) | 1998-07-09 | 2000-04-24 | 農林水産省食品総合研究所長 | マイクロキャピラリーアレイ、その製造方法、及び物質注入装置 |
| US6468657B1 (en) * | 1998-12-04 | 2002-10-22 | The Regents Of The University Of California | Controllable ion-exchange membranes |
| DE19905571C1 (de) | 1999-02-11 | 2000-11-16 | Bosch Gmbh Robert | Verfahren zur Erzeugung definiert konischer Löcher mittels eines Laserstrahls |
| US6555781B2 (en) | 1999-05-10 | 2003-04-29 | Nanyang Technological University | Ultrashort pulsed laser micromachining/submicromachining using an acoustooptic scanning device with dispersion compensation |
| US6300108B1 (en) | 1999-07-21 | 2001-10-09 | The Regents Of The University Of California | Controlled electroporation and mass transfer across cell membranes |
| KR100803186B1 (ko) | 2000-12-15 | 2008-02-14 | 디 아리조나 보드 오브 리전츠 | 나노입자 함유 전구체를 사용한 금속의 패턴형성 방법 |
| US20020099356A1 (en) | 2001-01-19 | 2002-07-25 | Unger Evan C. | Transmembrane transport apparatus and method |
| US6555161B1 (en) | 2001-05-18 | 2003-04-29 | Ensci Inc. | Process for producing thin film metal oxide coated substrates |
| CA2468424A1 (en) | 2001-11-27 | 2003-06-05 | Cecilia Farre | A method for combined parallel agent delivery and electroporation for cell structures and use thereof |
| AU2003233458A1 (en) | 2002-03-29 | 2003-10-13 | Array Bioscience Corporation | Methods for manufacturing nanoparticle structures using hydrophobic or charged surfaces |
| CA2428187C (en) * | 2002-05-08 | 2012-10-02 | National Research Council Of Canada | Method of fabricating sub-micron structures in transparent dielectric materials |
| JP5010793B2 (ja) | 2002-07-09 | 2012-08-29 | 独立行政法人科学技術振興機構 | 電気注入法を用いた動物細胞への細胞内導入物質の導入方法及びその装置 |
| US6737158B1 (en) | 2002-10-30 | 2004-05-18 | Gore Enterprise Holdings, Inc. | Porous polymeric membrane toughened composites |
| US7264723B2 (en) | 2002-11-01 | 2007-09-04 | Sandia Corporation | Dialysis on microchips using thin porous polymer membranes |
| US20040101822A1 (en) | 2002-11-26 | 2004-05-27 | Ulrich Wiesner | Fluorescent silica-based nanoparticles |
| US6962816B2 (en) | 2003-01-10 | 2005-11-08 | Reveo, Inc. | Highly controllable electroporation and applications thereof |
| US20060047254A1 (en) | 2003-04-04 | 2006-03-02 | Ravi Nallakrishnan | Phacoemulsification needle |
| US20050118102A1 (en) | 2003-04-28 | 2005-06-02 | Intematix Corporation | Spin resonance heating and/or imaging in medical applications |
| US7306823B2 (en) | 2004-09-18 | 2007-12-11 | Nanosolar, Inc. | Coated nanoparticles and quantum dots for solution-based fabrication of photovoltaic cells |
| US7824620B2 (en) | 2004-09-21 | 2010-11-02 | The Trustees Of The University Of Pennsylvania | Nano- and micro-scale structures: methods, devices and applications thereof |
| JP2006149227A (ja) | 2004-11-25 | 2006-06-15 | Fujitsu Ltd | 微小物体の捕獲装置及び方法 |
| WO2006116021A2 (en) | 2005-04-22 | 2006-11-02 | Intematix Corporation | Mri technique based on electron spin resonance and endohedral contrast agent |
| US7666494B2 (en) | 2005-05-04 | 2010-02-23 | 3M Innovative Properties Company | Microporous article having metallic nanoparticle coating |
| GB0512216D0 (en) | 2005-06-15 | 2005-07-27 | Capsant Neurotechnologies Ltd | Device |
| WO2007008609A2 (en) | 2005-07-07 | 2007-01-18 | The Regents Of The University Of California | Methods and apparatus for cell culture array |
| WO2007142674A2 (en) | 2005-10-27 | 2007-12-13 | Massachusetts Institute Of Technology | Nanoparticle heating and applications thereof |
| US20070173470A1 (en) | 2006-01-23 | 2007-07-26 | Chi-Hung Lin | Methods for delivering extracellular target into cells |
| US9149564B2 (en) * | 2006-06-23 | 2015-10-06 | The Regents Of The University Of California | Articles comprising large-surface-area bio-compatible materials and methods for making and using them |
| CA2671806A1 (en) | 2006-12-08 | 2008-06-19 | Austin M. Derfus | Remotely triggered release from heatable surfaces |
| US20100040549A1 (en) * | 2007-01-05 | 2010-02-18 | William Marsh Rice University | Composition for Targeted Drug Delivery and Controlled Release |
| JP2008238184A (ja) | 2007-03-26 | 2008-10-09 | Mitsubishi Electric Corp | レーザ加工装置 |
| JP5205798B2 (ja) | 2007-04-27 | 2013-06-05 | 富士通株式会社 | マイクロインジェクション装置、捕捉プレート、およびマイクロインジェクション方法 |
| US8008063B2 (en) * | 2008-06-24 | 2011-08-30 | Bio-Rad Laboratories, Inc. | Individual-cell electroporation using area-focused electric fields |
| JP2011067176A (ja) | 2009-09-28 | 2011-04-07 | Saitama Univ | 圧力変化を利用する動物細胞への物質導入 |
| US20120160095A1 (en) * | 2010-11-29 | 2012-06-28 | The Regents Of The University Of Colorado, A Body Corporate | Novel Nanoporous Supported Lyotropic Liquid Crystal Polymer Membranes and Methods of Preparing and Using Same |
| US20120245042A1 (en) | 2011-03-14 | 2012-09-27 | The Trustees Of The University Of Pennsylvania | Debubbler for microfluidic systems |
| JP6606058B2 (ja) | 2013-03-15 | 2019-11-13 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 光熱プラットフォームを使用する生細胞への高スループットカーゴ送達 |
| WO2015148842A1 (en) | 2014-03-28 | 2015-10-01 | The Regents Of The University Of California | Efficient delivery of large cargos into cells on a porous substrate |
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2014
- 2014-03-13 JP JP2016502195A patent/JP6606058B2/ja active Active
- 2014-03-13 WO PCT/US2014/026618 patent/WO2014151888A1/en not_active Ceased
- 2014-03-13 EP EP14768087.0A patent/EP2971011B1/en active Active
- 2014-03-13 CN CN201480015382.3A patent/CN105452471A/zh active Pending
- 2014-03-13 CA CA2905999A patent/CA2905999A1/en not_active Abandoned
- 2014-03-13 US US14/771,478 patent/US10982217B2/en active Active
- 2014-03-13 AU AU2014236747A patent/AU2014236747B2/en active Active
- 2014-03-13 KR KR1020157027342A patent/KR102202742B1/ko active Active
-
2015
- 2015-09-03 IL IL241150A patent/IL241150B/en active IP Right Grant
-
2021
- 2021-03-16 US US17/203,620 patent/US12391949B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009017695A1 (en) | 2007-07-26 | 2009-02-05 | The Regents Of The University Of California | A single cell surgery tool and a cell transfection device utilizing the photothermal properties of thin films and/or metal nanoparticles |
| WO2012158631A2 (en) * | 2011-05-13 | 2012-11-22 | The Regents Of The University Of California | Photothermal substrates for selective transfection of cells |
Non-Patent Citations (2)
| Title |
|---|
| Anal Chem.,83(4):1321-1327(2011.1.) |
| Optics Express.,18(22):23153-23160(2010.10.) |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2014236747A1 (en) | 2015-09-24 |
| CN105452471A (zh) | 2016-03-30 |
| IL241150A0 (en) | 2015-11-30 |
| US10982217B2 (en) | 2021-04-20 |
| AU2014236747B2 (en) | 2019-11-07 |
| US20160017340A1 (en) | 2016-01-21 |
| EP2971011A1 (en) | 2016-01-20 |
| US20210207150A1 (en) | 2021-07-08 |
| JP2016512039A (ja) | 2016-04-25 |
| WO2014151888A1 (en) | 2014-09-25 |
| EP2971011A4 (en) | 2016-10-26 |
| KR20150130351A (ko) | 2015-11-23 |
| US12391949B2 (en) | 2025-08-19 |
| CA2905999A1 (en) | 2014-09-25 |
| EP2971011B1 (en) | 2021-11-10 |
| IL241150B (en) | 2021-02-28 |
| JP6606058B2 (ja) | 2019-11-13 |
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