CA2905999A1 - High-throughput cargo delivery into live cells using photothermal platforms - Google Patents

High-throughput cargo delivery into live cells using photothermal platforms Download PDF

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Publication number
CA2905999A1
CA2905999A1 CA2905999A CA2905999A CA2905999A1 CA 2905999 A1 CA2905999 A1 CA 2905999A1 CA 2905999 A CA2905999 A CA 2905999A CA 2905999 A CA2905999 A CA 2905999A CA 2905999 A1 CA2905999 A1 CA 2905999A1
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CA
Canada
Prior art keywords
cells
reagent
thin film
substrate
orifices
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2905999A
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English (en)
French (fr)
Inventor
Yi-Chien WU
Ting-Hsiang S. Wu
Pei-Yu E. Chiou
Michael A. Teitell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California San Diego UCSD
Original Assignee
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Publication of CA2905999A1 publication Critical patent/CA2905999A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2905999A 2013-03-15 2014-03-13 High-throughput cargo delivery into live cells using photothermal platforms Abandoned CA2905999A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361799222P 2013-03-15 2013-03-15
US61/799,222 2013-03-15
PCT/US2014/026618 WO2014151888A1 (en) 2013-03-15 2014-03-13 High-throughput cargo delivery into live cells using photothermal platforms

Publications (1)

Publication Number Publication Date
CA2905999A1 true CA2905999A1 (en) 2014-09-25

Family

ID=51581064

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2905999A Abandoned CA2905999A1 (en) 2013-03-15 2014-03-13 High-throughput cargo delivery into live cells using photothermal platforms

Country Status (9)

Country Link
US (2) US10982217B2 (enExample)
EP (1) EP2971011B1 (enExample)
JP (1) JP6606058B2 (enExample)
KR (1) KR102202742B1 (enExample)
CN (1) CN105452471A (enExample)
AU (1) AU2014236747B2 (enExample)
CA (1) CA2905999A1 (enExample)
IL (1) IL241150B (enExample)
WO (1) WO2014151888A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2873204C (en) 2011-05-13 2021-10-19 The Regents Of The University Of California Photothermal substrates for selective transfection of cells
ES2764105T3 (es) 2011-10-17 2020-06-02 Massachusetts Inst Technology Administración intracelular
US11967839B2 (en) 2011-12-30 2024-04-23 Analog Devices, Inc. Electromagnetic connector for an industrial control system
US10834094B2 (en) 2013-08-06 2020-11-10 Bedrock Automation Platforms Inc. Operator action authentication in an industrial control system
US8862802B2 (en) 2011-12-30 2014-10-14 Bedrock Automation Platforms Inc. Switch fabric having a serial communications interface and a parallel communications interface
US11144630B2 (en) 2011-12-30 2021-10-12 Bedrock Automation Platforms Inc. Image capture devices for a secure industrial control system
US9467297B2 (en) 2013-08-06 2016-10-11 Bedrock Automation Platforms Inc. Industrial control system redundant communications/control modules authentication
US9449756B2 (en) * 2013-05-02 2016-09-20 Bedrock Automation Platforms Inc. Electromagnetic connectors
US12061685B2 (en) 2011-12-30 2024-08-13 Analog Devices, Inc. Image capture devices for a secure industrial control system
US8868813B2 (en) 2011-12-30 2014-10-21 Bedrock Automation Platforms Inc. Communications control system with a serial communications interface and a parallel communications interface
US11314854B2 (en) 2011-12-30 2022-04-26 Bedrock Automation Platforms Inc. Image capture devices for a secure industrial control system
US10834820B2 (en) 2013-08-06 2020-11-10 Bedrock Automation Platforms Inc. Industrial control system cable
US9600434B1 (en) 2011-12-30 2017-03-21 Bedrock Automation Platforms, Inc. Switch fabric having a serial communications interface and a parallel communications interface
US9437967B2 (en) 2011-12-30 2016-09-06 Bedrock Automation Platforms, Inc. Electromagnetic connector for an industrial control system
US8971072B2 (en) 2011-12-30 2015-03-03 Bedrock Automation Platforms Inc. Electromagnetic connector for an industrial control system
US9727511B2 (en) 2011-12-30 2017-08-08 Bedrock Automation Platforms Inc. Input/output module with multi-channel switching capability
US9191203B2 (en) 2013-08-06 2015-11-17 Bedrock Automation Platforms Inc. Secure industrial control system
JP6606058B2 (ja) 2013-03-15 2019-11-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 光熱プラットフォームを使用する生細胞への高スループットカーゴ送達
US10613567B2 (en) 2013-08-06 2020-04-07 Bedrock Automation Platforms Inc. Secure power supply for an industrial control system
KR102304167B1 (ko) 2013-08-16 2021-09-24 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
WO2015148842A1 (en) 2014-03-28 2015-10-01 The Regents Of The University Of California Efficient delivery of large cargos into cells on a porous substrate
CN111293495B (zh) 2014-07-07 2022-05-24 基岩自动化平台公司 工业控制系统电缆
US11111472B2 (en) 2014-10-31 2021-09-07 Massachusetts Institute Of Technology Delivery of biomolecules to immune cells
CN113897285A (zh) 2014-11-14 2022-01-07 麻省理工学院 化合物和组合物向细胞中的破坏和场实现的递送
US11125739B2 (en) 2015-01-12 2021-09-21 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
JP6090891B1 (ja) 2015-06-01 2017-03-08 株式会社片岡製作所 細胞処理方法、レーザ加工機、細胞培養容器
EP4257675A3 (en) 2015-07-09 2024-01-03 Massachusetts Institute of Technology Delivery of materials to anucleate cells
CN105420278A (zh) * 2015-12-09 2016-03-23 苏州大学 采用光致穿孔的方式制备负载有外源分子细胞的方法及制备该细胞用的基材及该细胞
CN105670620B (zh) * 2016-03-14 2017-11-14 山东农业大学 一种掺杂氮化碳荧光量子点的制备方法
US20190264229A1 (en) * 2016-10-21 2019-08-29 Agency For Science, Technology And Research Device, system and method for intracellular delivery of substances
WO2019157316A2 (en) * 2018-02-12 2019-08-15 Flagship Pioneering Innovations V, Inc. Methods and devices for the isolation of subcellular components
CN109142293A (zh) * 2018-07-27 2019-01-04 福州大学 一种基于氮化碳解吸附作用的荧光传感器及其制备和应用
KR102108023B1 (ko) * 2018-09-28 2020-05-07 포항공과대학교 산학협력단 이작용성 나노자임 및 이의 용도
US12496274B2 (en) 2018-11-14 2025-12-16 Flagship Pioneering Innovations V, Inc. Compositions and methods for compartment-specific cargo delivery
CN110261365B (zh) * 2019-07-02 2021-04-02 吉林大学 一种周期月牙形纳米间隙阵列及其制备方法
US10895727B1 (en) * 2019-10-19 2021-01-19 SequLITE Genomics US, Inc. Microscope for locating structures on the inner surface of a fluidic channel
AU2020366521B2 (en) * 2019-10-19 2024-03-07 SequLITE Genomics US, Inc. Virtual fiducials
WO2021109132A1 (zh) * 2019-12-06 2021-06-10 百脉迪生物科技(苏州)有限公司 一种复合材料及其制备方法和应用
WO2021178477A1 (en) * 2020-03-03 2021-09-10 Arizona Board Of Regents On Behalf Of Arizona State University Low-capacitance nanopore sensors on insulating substrates
WO2021251979A1 (en) * 2020-06-12 2021-12-16 Hewlett-Packard Development Company, L.P. Microfluidic passage with protective layer
WO2022031700A1 (en) * 2020-08-03 2022-02-10 The Regents Of The University Of California Laser-actuated supercritical injector
CN112461807B (zh) * 2020-11-26 2021-11-19 山西大学 碳量子点在靶向细胞核仁免洗成像中的应用
CN115888790B (zh) * 2022-11-14 2024-10-15 南京先进生物材料与过程装备研究院有限公司 一种利用微通道制备氮化碳纳米片的方法
WO2024233806A2 (en) * 2023-05-09 2024-11-14 Met Biotechnology, Inc. Methods of engineering cells with mutated genomes using intact large cargo transfer systems
WO2025117977A1 (en) * 2023-11-30 2025-06-05 Met Biotechnology, Inc. Electroporation systems and methods for cargo loading into extracellular vesicles

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310674A (en) 1982-05-10 1994-05-10 Bar-Ilan University Apertured cell carrier
JPH01141582A (ja) 1987-11-27 1989-06-02 Shimadzu Corp 遺伝子導入装置
US4917462A (en) 1988-06-15 1990-04-17 Cornell Research Foundation, Inc. Near field scanning optical microscopy
US5080586A (en) 1990-09-24 1992-01-14 Osada Research Institute, Ltd. Apical foramen position detector for use in dental treatment
JP2783984B2 (ja) 1995-04-21 1998-08-06 プリマハム株式会社 マイクロマニピュレーション装置及びそれを用いた細胞操作方法
US5922687A (en) 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
US6052286A (en) * 1997-04-11 2000-04-18 Texas Instruments Incorporated Restrained center core anisotropically conductive adhesive
WO1999044638A1 (en) 1998-03-06 1999-09-10 Spectrx, Inc. Photothermal structure for biomedical applications, and method therefor
US6537800B1 (en) 1998-03-12 2003-03-25 Center For Advanced Science And Technology Incubation, Ltd. Apparatus for automatically measuring minute membrane potential
AU3277199A (en) 1998-03-12 1999-09-27 Isao Karube Techniques for piercing specific site of cell
JP3035608B2 (ja) 1998-07-09 2000-04-24 農林水産省食品総合研究所長 マイクロキャピラリーアレイ、その製造方法、及び物質注入装置
US6468657B1 (en) * 1998-12-04 2002-10-22 The Regents Of The University Of California Controllable ion-exchange membranes
DE19905571C1 (de) 1999-02-11 2000-11-16 Bosch Gmbh Robert Verfahren zur Erzeugung definiert konischer Löcher mittels eines Laserstrahls
US6555781B2 (en) 1999-05-10 2003-04-29 Nanyang Technological University Ultrashort pulsed laser micromachining/submicromachining using an acoustooptic scanning device with dispersion compensation
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
KR100803186B1 (ko) 2000-12-15 2008-02-14 디 아리조나 보드 오브 리전츠 나노입자 함유 전구체를 사용한 금속의 패턴형성 방법
US20020099356A1 (en) 2001-01-19 2002-07-25 Unger Evan C. Transmembrane transport apparatus and method
US6555161B1 (en) 2001-05-18 2003-04-29 Ensci Inc. Process for producing thin film metal oxide coated substrates
CA2468424A1 (en) 2001-11-27 2003-06-05 Cecilia Farre A method for combined parallel agent delivery and electroporation for cell structures and use thereof
AU2003233458A1 (en) 2002-03-29 2003-10-13 Array Bioscience Corporation Methods for manufacturing nanoparticle structures using hydrophobic or charged surfaces
CA2428187C (en) * 2002-05-08 2012-10-02 National Research Council Of Canada Method of fabricating sub-micron structures in transparent dielectric materials
JP5010793B2 (ja) 2002-07-09 2012-08-29 独立行政法人科学技術振興機構 電気注入法を用いた動物細胞への細胞内導入物質の導入方法及びその装置
US6737158B1 (en) 2002-10-30 2004-05-18 Gore Enterprise Holdings, Inc. Porous polymeric membrane toughened composites
US7264723B2 (en) 2002-11-01 2007-09-04 Sandia Corporation Dialysis on microchips using thin porous polymer membranes
US20040101822A1 (en) 2002-11-26 2004-05-27 Ulrich Wiesner Fluorescent silica-based nanoparticles
US6962816B2 (en) 2003-01-10 2005-11-08 Reveo, Inc. Highly controllable electroporation and applications thereof
US20060047254A1 (en) 2003-04-04 2006-03-02 Ravi Nallakrishnan Phacoemulsification needle
US20050118102A1 (en) 2003-04-28 2005-06-02 Intematix Corporation Spin resonance heating and/or imaging in medical applications
US7306823B2 (en) 2004-09-18 2007-12-11 Nanosolar, Inc. Coated nanoparticles and quantum dots for solution-based fabrication of photovoltaic cells
US7824620B2 (en) 2004-09-21 2010-11-02 The Trustees Of The University Of Pennsylvania Nano- and micro-scale structures: methods, devices and applications thereof
JP2006149227A (ja) 2004-11-25 2006-06-15 Fujitsu Ltd 微小物体の捕獲装置及び方法
WO2006116021A2 (en) 2005-04-22 2006-11-02 Intematix Corporation Mri technique based on electron spin resonance and endohedral contrast agent
US7666494B2 (en) 2005-05-04 2010-02-23 3M Innovative Properties Company Microporous article having metallic nanoparticle coating
GB0512216D0 (en) 2005-06-15 2005-07-27 Capsant Neurotechnologies Ltd Device
WO2007008609A2 (en) 2005-07-07 2007-01-18 The Regents Of The University Of California Methods and apparatus for cell culture array
WO2007142674A2 (en) 2005-10-27 2007-12-13 Massachusetts Institute Of Technology Nanoparticle heating and applications thereof
US20070173470A1 (en) 2006-01-23 2007-07-26 Chi-Hung Lin Methods for delivering extracellular target into cells
US9149564B2 (en) * 2006-06-23 2015-10-06 The Regents Of The University Of California Articles comprising large-surface-area bio-compatible materials and methods for making and using them
CA2671806A1 (en) 2006-12-08 2008-06-19 Austin M. Derfus Remotely triggered release from heatable surfaces
US20100040549A1 (en) * 2007-01-05 2010-02-18 William Marsh Rice University Composition for Targeted Drug Delivery and Controlled Release
JP2008238184A (ja) 2007-03-26 2008-10-09 Mitsubishi Electric Corp レーザ加工装置
JP5205798B2 (ja) 2007-04-27 2013-06-05 富士通株式会社 マイクロインジェクション装置、捕捉プレート、およびマイクロインジェクション方法
WO2009017695A1 (en) 2007-07-26 2009-02-05 The Regents Of The University Of California A single cell surgery tool and a cell transfection device utilizing the photothermal properties of thin films and/or metal nanoparticles
US8008063B2 (en) * 2008-06-24 2011-08-30 Bio-Rad Laboratories, Inc. Individual-cell electroporation using area-focused electric fields
JP2011067176A (ja) 2009-09-28 2011-04-07 Saitama Univ 圧力変化を利用する動物細胞への物質導入
US20120160095A1 (en) * 2010-11-29 2012-06-28 The Regents Of The University Of Colorado, A Body Corporate Novel Nanoporous Supported Lyotropic Liquid Crystal Polymer Membranes and Methods of Preparing and Using Same
US20120245042A1 (en) 2011-03-14 2012-09-27 The Trustees Of The University Of Pennsylvania Debubbler for microfluidic systems
CA2873204C (en) * 2011-05-13 2021-10-19 The Regents Of The University Of California Photothermal substrates for selective transfection of cells
JP6606058B2 (ja) 2013-03-15 2019-11-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 光熱プラットフォームを使用する生細胞への高スループットカーゴ送達
WO2015148842A1 (en) 2014-03-28 2015-10-01 The Regents Of The University Of California Efficient delivery of large cargos into cells on a porous substrate

Also Published As

Publication number Publication date
AU2014236747A1 (en) 2015-09-24
CN105452471A (zh) 2016-03-30
IL241150A0 (en) 2015-11-30
US10982217B2 (en) 2021-04-20
AU2014236747B2 (en) 2019-11-07
US20160017340A1 (en) 2016-01-21
EP2971011A1 (en) 2016-01-20
US20210207150A1 (en) 2021-07-08
JP2016512039A (ja) 2016-04-25
WO2014151888A1 (en) 2014-09-25
EP2971011A4 (en) 2016-10-26
KR20150130351A (ko) 2015-11-23
US12391949B2 (en) 2025-08-19
EP2971011B1 (en) 2021-11-10
IL241150B (en) 2021-02-28
KR102202742B1 (ko) 2021-01-12
JP6606058B2 (ja) 2019-11-13

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