JP6606058B2 - 光熱プラットフォームを使用する生細胞への高スループットカーゴ送達 - Google Patents

光熱プラットフォームを使用する生細胞への高スループットカーゴ送達 Download PDF

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JP6606058B2
JP6606058B2 JP2016502195A JP2016502195A JP6606058B2 JP 6606058 B2 JP6606058 B2 JP 6606058B2 JP 2016502195 A JP2016502195 A JP 2016502195A JP 2016502195 A JP2016502195 A JP 2016502195A JP 6606058 B2 JP6606058 B2 JP 6606058B2
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cells
cell
thin film
porous polymer
reagent
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JP2016512039A5 (enExample
JP2016512039A (ja
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イ−チエン・ウー
ティン−シアン・エス・ウー
ペイ−ユ・イー・チョウ
マイケル・エー・テイテル
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University of California
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2016502195A 2013-03-15 2014-03-13 光熱プラットフォームを使用する生細胞への高スループットカーゴ送達 Active JP6606058B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361799222P 2013-03-15 2013-03-15
US61/799,222 2013-03-15
PCT/US2014/026618 WO2014151888A1 (en) 2013-03-15 2014-03-13 High-throughput cargo delivery into live cells using photothermal platforms

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JP2016512039A JP2016512039A (ja) 2016-04-25
JP2016512039A5 JP2016512039A5 (enExample) 2017-04-20
JP6606058B2 true JP6606058B2 (ja) 2019-11-13

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US (2) US10982217B2 (enExample)
EP (1) EP2971011B1 (enExample)
JP (1) JP6606058B2 (enExample)
KR (1) KR102202742B1 (enExample)
CN (1) CN105452471A (enExample)
AU (1) AU2014236747B2 (enExample)
CA (1) CA2905999A1 (enExample)
IL (1) IL241150B (enExample)
WO (1) WO2014151888A1 (enExample)

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AU2014236747B2 (en) 2019-11-07
KR102202742B1 (ko) 2021-01-12
EP2971011B1 (en) 2021-11-10
US20210207150A1 (en) 2021-07-08
IL241150A0 (en) 2015-11-30
EP2971011A1 (en) 2016-01-20
US12391949B2 (en) 2025-08-19
JP2016512039A (ja) 2016-04-25
US20160017340A1 (en) 2016-01-21
EP2971011A4 (en) 2016-10-26
CN105452471A (zh) 2016-03-30
AU2014236747A1 (en) 2015-09-24
US10982217B2 (en) 2021-04-20
CA2905999A1 (en) 2014-09-25
IL241150B (en) 2021-02-28
WO2014151888A1 (en) 2014-09-25
KR20150130351A (ko) 2015-11-23

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