KR102001427B1 - 스트렙토코쿠스 피오게네스 유래의 엔도글리코시다아제 및 그의 이용방법 - Google Patents

스트렙토코쿠스 피오게네스 유래의 엔도글리코시다아제 및 그의 이용방법 Download PDF

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KR102001427B1
KR102001427B1 KR1020147009205A KR20147009205A KR102001427B1 KR 102001427 B1 KR102001427 B1 KR 102001427B1 KR 1020147009205 A KR1020147009205 A KR 1020147009205A KR 20147009205 A KR20147009205 A KR 20147009205A KR 102001427 B1 KR102001427 B1 KR 102001427B1
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endos49
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polypeptide
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KR20140081813A (ko
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마티아스 콜린
마리아 알혼
죠나단 쉐그렌
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게노비스 에이비
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56944—Streptococcus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020147009205A 2011-09-13 2012-09-12 스트렙토코쿠스 피오게네스 유래의 엔도글리코시다아제 및 그의 이용방법 Active KR102001427B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1115841.7 2011-09-13
GB201115841A GB201115841D0 (en) 2011-09-13 2011-09-13 Protein and method
PCT/EP2012/067841 WO2013037824A1 (en) 2011-09-13 2012-09-12 Endoglycosidase from streptococcus pyogenes and methods using it

Publications (2)

Publication Number Publication Date
KR20140081813A KR20140081813A (ko) 2014-07-01
KR102001427B1 true KR102001427B1 (ko) 2019-07-18

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Country Link
US (1) US9493752B2 (https=)
EP (1) EP2756077B1 (https=)
JP (1) JP6076349B2 (https=)
KR (1) KR102001427B1 (https=)
CN (1) CN103946377B (https=)
AU (1) AU2012307461B2 (https=)
BR (1) BR112014005816A2 (https=)
CA (1) CA2848230C (https=)
DK (1) DK2756077T3 (https=)
EA (1) EA028490B8 (https=)
ES (1) ES2627334T3 (https=)
GB (2) GB201115841D0 (https=)
IL (1) IL231410A0 (https=)
IN (1) IN2014CN02163A (https=)
MX (1) MX347727B (https=)
PL (1) PL2756077T3 (https=)
PT (1) PT2756077T (https=)
SG (1) SG11201400505YA (https=)
WO (1) WO2013037824A1 (https=)
ZA (1) ZA201401840B (https=)

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KR20170110153A (ko) * 2015-02-12 2017-10-10 한사 메디컬 에이비 시스테인 프로테아제

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EP1343895B1 (en) * 2000-12-21 2008-06-18 ID Biomedical Corporation Streptococcus pyogenes antigens and corresponding dna fragments
FI2911699T4 (fi) 2012-10-23 2025-12-09 Synaffix Bv Muokattu vasta-aine, vasta-ainekonjugaatti ja menetelmä niiden valmistamiseksi
PL2991683T3 (pl) 2013-05-02 2020-03-31 Glykos Finland Oy Koniugaty glikoproteiny lub glikanu z toksycznym ładunkiem
EP3057618B1 (en) 2013-10-14 2022-12-14 SynAffix B.V. Glycoengineered antibody, antibody-conjugate and methods for their preparation
WO2015057066A1 (en) 2013-10-14 2015-04-23 Synaffix B.V. Glycoengineered antibody, antibody-conjugate and methods for their preparation
EP2935608A1 (en) 2013-10-14 2015-10-28 SynAffix B.V. Modified glycoprotein, protein-conjugate and process for the preparation thereof
EP3058083B1 (en) 2013-10-14 2018-04-11 SynAffix B.V. Modified glycoprotein, protein-conjugate and process for the preparation thereof
GB201318490D0 (en) * 2013-10-18 2013-12-04 Genovis Ab Method
CN106660967A (zh) 2014-01-24 2017-05-10 西纳福克斯股份有限公司 (杂)芳基1，3‑偶极化合物与(杂)环炔的环加成方法
EP3160513B1 (en) 2014-06-30 2020-02-12 Glykos Finland Oy Saccharide derivative of a toxic payload and antibody conjugates thereof
GB201413240D0 (en) * 2014-07-25 2014-09-10 Hansa Medical Ab Method
EP3177633B1 (en) 2014-08-04 2022-07-20 SynAffix B.V. Process for the modification of a glycoprotein using a beta-(1,4)-n-acetylgalactosaminyltransferase or a mutant thereof
GB201502306D0 (en) * 2015-02-12 2015-04-01 Hansa Medical Ab Protein
CN107810273B (zh) 2015-04-23 2022-09-27 西纳福克斯股份有限公司 用为或衍生自β-(1,4)-N-乙酰半乳糖胺转移酶的糖基转移酶修饰糖蛋白的方法
EP3402815B1 (en) 2016-01-15 2023-12-06 University of Maryland, College Park Endo-s2 mutants as glycosynthases, method of making and use for glycoengineering of glycoproteins
ES2877754T3 (es) 2016-02-04 2021-11-17 Genovis Ab Nuevas proteasas estreptocócicas
WO2018039373A1 (en) * 2016-08-24 2018-03-01 Cho Pharma Inc Endoglycosidase mutants for glycoprotein remodeling and methods of using it
DK3630968T3 (da) 2017-05-26 2024-07-29 Genovis Ab Enzymer til glycananalyse
US20210228738A1 (en) 2017-07-17 2021-07-29 INSERM (Institut National de la Santé et de la Recherche Médicale) Compositions and methods for increasing or enhancing transduction of gene therapy vectors and for removing or reducing immunoglobulins
WO2019243672A1 (en) 2018-06-19 2019-12-26 Glykos Biomedical Oy Conjugate
US20210138080A1 (en) 2018-06-29 2021-05-13 Glykos Biomedical Oy Conjugates
US20230038373A1 (en) 2019-12-18 2023-02-09 Glykos Biomedical Oy Stabile conjugate
CN111044731B (zh) * 2019-12-19 2021-05-07 北京科技大学 脉冲温育免疫反应分离富集多肽药物中肽类杂质的方法
IL294321B1 (en) 2020-01-09 2026-04-01 Mersana Therapeutics Inc Antibody-drug conjugates are attached at a specific site with linkers that include peptides.
CN120078887A (zh) * 2020-09-21 2025-06-03 上海宝济药业股份有限公司 一种药物组合及其应用
JP2024501894A (ja) 2021-01-04 2024-01-16 メルサナ セラピューティクス インコーポレイテッド Ｂ７ｈ４ターゲティング抗体－薬物コンジュゲートおよびその使用方法
EP4079848A1 (en) 2021-04-22 2022-10-26 Genovis Ab Immunoglobulin cleaving enzyme
CN115873833B (zh) * 2022-11-08 2024-08-16 上海泰昶生物技术有限公司 生产免疫球蛋白g降解酶的工程菌株和工艺
CN117165643B (zh) * 2023-08-17 2025-10-28 山东大学 一种糖苷内切酶在水解高甘露糖型n-糖蛋白中的应用
TW202519652A (zh) * 2023-11-10 2025-05-16 大陸商上海齊魯製藥研究中心有限公司 新型Endo S2突變體酶
TW202540414A (zh) * 2024-03-01 2025-10-16 大陸商上海齊魯製藥研究中心有限公司 新型Endo S2突變體酶
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US20100135981A1 (en) 2006-12-13 2010-06-03 Hansa Medical Ab Use of the endoglycosidase endos for treating immunoglobulin g mediated diseases
WO2009033670A2 (en) 2007-09-14 2009-03-19 Genovis Ab Method and kits for dissociating fcgamma-receptor-igg complexes and for igg purification and detection
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170110153A (ko) * 2015-02-12 2017-10-10 한사 메디컬 에이비 시스테인 프로테아제
KR102524594B1 (ko) 2015-02-12 2023-04-21 한사 바이오파마 에이비 시스테인 프로테아제

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CA2848230C (en) 2020-03-24
ZA201401840B (en) 2018-05-30
EP2756077B1 (en) 2017-03-29
GB2509284A (en) 2014-06-25
DK2756077T3 (en) 2017-07-03
KR20140081813A (ko) 2014-07-01
IL231410A0 (en) 2014-04-30
PT2756077T (pt) 2017-06-21
CN103946377B (zh) 2016-10-05
IN2014CN02163A (https=) 2015-05-29
ES2627334T3 (es) 2017-07-27
GB201115841D0 (en) 2011-10-26
EA028490B1 (ru) 2017-11-30
SG11201400505YA (en) 2014-04-28
JP6076349B2 (ja) 2017-02-08
US20140302519A1 (en) 2014-10-09
NZ622172A (en) 2016-04-29
AU2012307461B2 (en) 2016-08-18
JP2014531205A (ja) 2014-11-27
CA2848230A1 (en) 2013-03-21
CN103946377A (zh) 2014-07-23
WO2013037824A1 (en) 2013-03-21
MX2014002906A (es) 2014-10-17
GB201406321D0 (en) 2014-05-21
MX347727B (es) 2017-05-10
EP2756077A1 (en) 2014-07-23
AU2012307461A1 (en) 2014-03-27
PL2756077T3 (pl) 2017-09-29
BR112014005816A2 (pt) 2020-12-29
US9493752B2 (en) 2016-11-15
EA201490447A1 (ru) 2014-09-30
EA028490B8 (ru) 2018-01-31

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