KR101653555B1 - 단백질의 분비 생산 방법 - Google Patents

단백질의 분비 생산 방법 Download PDF

Info

Publication number
KR101653555B1
KR101653555B1 KR1020147015040A KR20147015040A KR101653555B1 KR 101653555 B1 KR101653555 B1 KR 101653555B1 KR 1020147015040 A KR1020147015040 A KR 1020147015040A KR 20147015040 A KR20147015040 A KR 20147015040A KR 101653555 B1 KR101653555 B1 KR 101653555B1
Authority
KR
South Korea
Prior art keywords
protein
ala
ser
thr
leu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147015040A
Other languages
English (en)
Korean (ko)
Other versions
KR20140088215A (ko
Inventor
요시히코 마쓰다
히로시 이타야
요시미 기쿠치
하루키 벳푸
유르기스 안타나스 블라도비치 요만타스
에카테리나 알렉산드로브나 쿠투코바
Original Assignee
아지노모토 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from RU2011144497/10A external-priority patent/RU2011144497A/ru
Application filed by 아지노모토 가부시키가이샤 filed Critical 아지노모토 가부시키가이샤
Publication of KR20140088215A publication Critical patent/KR20140088215A/ko
Application granted granted Critical
Publication of KR101653555B1 publication Critical patent/KR101653555B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147015040A 2011-11-02 2012-11-01 단백질의 분비 생산 방법 Active KR101653555B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2011240745 2011-11-02
JPJP-P-2011-240745 2011-11-02
RU2011144497 2011-11-02
RU2011144497/10A RU2011144497A (ru) 2011-11-02 2011-11-02 Способ секреторного получения белка
PCT/JP2012/078906 WO2013065869A1 (en) 2011-11-02 2012-11-01 Method for secretory production of protein

Publications (2)

Publication Number Publication Date
KR20140088215A KR20140088215A (ko) 2014-07-09
KR101653555B1 true KR101653555B1 (ko) 2016-09-02

Family

ID=47222244

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147015040A Active KR101653555B1 (ko) 2011-11-02 2012-11-01 단백질의 분비 생산 방법

Country Status (8)

Country Link
US (1) US9187554B2 (enExample)
EP (1) EP2748340B1 (enExample)
JP (1) JP6020581B2 (enExample)
KR (1) KR101653555B1 (enExample)
CN (1) CN103946371B (enExample)
AU (1) AU2012333451B2 (enExample)
IN (1) IN2014CN04039A (enExample)
WO (1) WO2013065869A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2014CN04039A (enExample) 2011-11-02 2015-10-23 Ajinomoto Kk
EP2774982B1 (en) 2011-11-02 2017-06-21 Ajinomoto Co., Inc. Method for secreting and producing proteins
RU2013119826A (ru) 2013-04-29 2014-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Коринеформная бактерия и способ получения гетерологичных гибридных белков
EP3061828B1 (en) 2013-10-23 2024-08-28 Ajinomoto Co., Inc. Method for producing target substance
US10344093B2 (en) 2014-05-02 2019-07-09 Shinshu University Antibody gene expression-secretion system
RU2017105849A (ru) * 2014-07-25 2018-08-29 Мемориал Слоан Кеттеринг Кэнсер Сентер Биспецифические молекулы, связывающие her2 и cd3
CN107532163A (zh) 2015-04-24 2018-01-02 味之素株式会社 蛋白质的分泌生产方法
KR102335301B1 (ko) 2016-10-21 2021-12-06 아지노모토 가부시키가이샤 단백질의 분비 생산법
KR102337932B1 (ko) * 2016-10-21 2021-12-13 아지노모토 가부시키가이샤 단백질의 분비 생산법
JP7613833B2 (ja) 2017-03-28 2025-01-15 味の素株式会社 Ｒｎａの製造方法
WO2019163827A1 (ja) 2018-02-20 2019-08-29 味の素株式会社 Ｒｎａサイレンシングを誘導する方法
JP7439749B2 (ja) 2018-04-20 2024-02-28 味の素株式会社 タンパク質の分泌生産法
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
AU2019364829B2 (en) 2018-10-25 2025-11-20 Ajinomoto Co., Inc. Method for secretory production of protein
CN111909879B (zh) * 2020-08-24 2023-04-28 江南大学 一种高效表达外源蛋白的谷氨酸棒杆菌诱变菌株及应用
US12180521B2 (en) 2021-02-19 2024-12-31 Theripion, Inc. Paraoxonase fusion polypeptides and related compositions and methods
AU2022306337A1 (en) 2021-07-07 2024-02-01 Ajinomoto Co., Inc. Method for secretory production of unnatural-amino-acid-containing protein
EP4509613A1 (en) 2022-04-15 2025-02-19 Ajinomoto Co., Inc. Method for producing cultured meat
AU2024221604A1 (en) 2023-02-16 2025-09-11 Ajinomoto Co., Inc. Method for secretory production of proteins

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57134500A (en) 1981-02-12 1982-08-19 Kyowa Hakko Kogyo Co Ltd Plasmid pcg1
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
JPS58192900A (ja) 1982-05-04 1983-11-10 Ajinomoto Co Inc 複合プラスミド
US4965197A (en) 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
JPH01191686A (ja) 1988-01-26 1989-08-01 Mitsubishi Petrochem Co Ltd 複合プラスミド
US5185262A (en) 1988-07-27 1993-02-09 Mitsubishi Petrochemical Co., Ltd. DNA fragment containing gene which encodes the function of stabilizing plasmid in host microorganism
JP2678995B2 (ja) 1988-09-08 1997-11-19 三菱化学株式会社 トリプトフアンシンターゼの製造法
JP2973446B2 (ja) 1990-01-11 1999-11-08 三菱化学株式会社 新規プラスミドベクター
JPH07108228B2 (ja) 1990-10-15 1995-11-22 味の素株式会社 温度感受性プラスミド
JP2925776B2 (ja) 1991-04-22 1999-07-28 三洋電機株式会社 吸収式冷凍機
EP0551506A1 (fr) 1991-07-30 1993-07-21 Orsan Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries
JP3720363B2 (ja) 1994-08-26 2005-11-24 ノボザイムス アクティーゼルスカブ 微生物のトランスグルタミナーゼ、それらの産生及び使用
ATE248219T1 (de) 1995-01-19 2003-09-15 Novozymes As Transglutaminasen aus oomyzeten
JPH0970291A (ja) 1995-06-30 1997-03-18 Ajinomoto Co Inc 人工トランスポゾンを用いた遺伝子増幅方法
JP4035855B2 (ja) 1996-06-05 2008-01-23 味の素株式会社 Ｌ−リジンの製造法
JP3711658B2 (ja) 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
JP4168463B2 (ja) 1996-12-05 2008-10-22 味の素株式会社 Ｌ−リジンの製造法
JPH11169182A (ja) 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
US7033802B1 (en) * 1998-03-06 2006-04-25 Ajinomoto Co., Inc. Penicillin binding protein gene and process for producing L-glutamic acid
JP2000262288A (ja) 1999-03-16 2000-09-26 Ajinomoto Co Inc コリネ型細菌の温度感受性プラスミド
US20050244935A1 (en) 1999-06-25 2005-11-03 Basf Ag Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
DE60042552D1 (de) 1999-08-02 2009-08-27 Archer Daniels Midland Co Metabolische manipulation der produktion von aminosäuren
KR100565030B1 (ko) 1999-09-30 2006-03-30 아지노모토 가부시키가이샤 트랜스글루타미나제의 제조방법
HU229834B1 (en) 2000-01-21 2014-09-29 Ajinomoto Kk Process for producing l-lysine
BR0208136B1 (pt) 2001-03-30 2014-10-21 Ajinomoto Kk Método para produzir uma proteína heteróloga
EP1548116A4 (en) * 2002-09-30 2006-04-05 Ajinomoto Kk PROCESS FOR PRODUCING A PROTEIN MARKED BY A STABLE ISOTOPE
CA2518049C (en) 2003-03-07 2013-05-07 Ajinomoto Co., Inc. Method of producing microbial transglutaminase
JP4705026B2 (ja) 2004-03-19 2011-06-22 ジェノミディア株式会社 血管内皮増殖促進遺伝子
KR101089559B1 (ko) 2004-03-31 2011-12-06 아지노모토 가부시키가이샤 바실러스 또는 에스케리키아 속에 속하는 세균을 사용하여 발효에 의해 퓨린 뉴클레오사이드 및 뉴클레오타이드를 제조하는 방법
EP1748077B1 (en) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Method of producing protein
JP5023701B2 (ja) 2004-05-20 2012-09-12 味の素株式会社 コハク酸生産菌及びコハク酸の製造方法
JP4595506B2 (ja) 2004-11-25 2010-12-08 味の素株式会社 Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造方法
AU2006219823A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US7723097B2 (en) 2005-03-11 2010-05-25 Archer-Daniels-Midland Company Escherichia coli strains that over-produce L-threonine and processes for their production
RU2365622C2 (ru) 2006-12-22 2009-08-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПРОДУКЦИИ ПУРИНОВЫХ НУКЛЕОЗИДОВ И НУКЛЕОТИДОВ МЕТОДОМ ФЕРМЕНТАЦИИ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ, ПРИНАДЛЕЖАЩИХ К РОДУ Escherichia ИЛИ Bacillus
CN102089437B (zh) 2008-07-09 2014-11-05 味之素株式会社 生产氨基羟基苯甲酸盐类化合物的方法
IN2014CN04039A (enExample) 2011-11-02 2015-10-23 Ajinomoto Kk
EP2774982B1 (en) 2011-11-02 2017-06-21 Ajinomoto Co., Inc. Method for secreting and producing proteins

Also Published As

Publication number Publication date
EP2748340A1 (en) 2014-07-02
KR20140088215A (ko) 2014-07-09
JP6020581B2 (ja) 2016-11-02
RU2014122201A (ru) 2015-12-10
CN103946371A (zh) 2014-07-23
CN103946371B (zh) 2016-07-06
WO2013065869A1 (en) 2013-05-10
JP2014532424A (ja) 2014-12-08
AU2012333451A1 (en) 2014-04-03
US20140255996A1 (en) 2014-09-11
IN2014CN04039A (enExample) 2015-10-23
EP2748340B1 (en) 2016-06-01
AU2012333451B2 (en) 2015-05-21
US9187554B2 (en) 2015-11-17

Similar Documents

Publication Publication Date Title
KR101653555B1 (ko) 단백질의 분비 생산 방법
EP2774982B1 (en) Method for secreting and producing proteins
US9376701B2 (en) Method for secretory production of protein
KR102337932B1 (ko) 단백질의 분비 생산법
KR102335301B1 (ko) 단백질의 분비 생산법
JP2023184699A (ja) タンパク質の分泌生産法
EP3872096A1 (en) Method for secretory production of protein
US10077447B2 (en) Coryneform bacterium and method for producing heterologous fusion proteins
RU2588439C2 (ru) Способ секреторной продукции белка

Legal Events

Date Code Title Description
A201 Request for examination
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 9

R18-X000 Changes to party contact information recorded

St.27 status event code: A-5-5-R10-R18-oth-X000

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 10