KR101604502B1 - 동물세포를 사용해서 외래유전자 유래 단백질을 대량으로 생산하기 위한 발현 벡터, 및 그의 이용 - Google Patents

동물세포를 사용해서 외래유전자 유래 단백질을 대량으로 생산하기 위한 발현 벡터, 및 그의 이용 Download PDF

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KR101604502B1
KR101604502B1 KR1020107021057A KR20107021057A KR101604502B1 KR 101604502 B1 KR101604502 B1 KR 101604502B1 KR 1020107021057 A KR1020107021057 A KR 1020107021057A KR 20107021057 A KR20107021057 A KR 20107021057A KR 101604502 B1 KR101604502 B1 KR 101604502B1
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gene
expression vector
promoter
foreign gene
transformant
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KR20100135755A (ko
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야스히코 스즈키
게이이치 야마모토
히로시 다하라
유스케 스즈키
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국립대학법인 홋가이도 다이가쿠
후소 야쿠힝 고교 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
    • C12N2840/102—Vectors comprising a special translation-regulating system regulates levels of translation inhibiting translation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020107021057A 2008-02-27 2009-02-27 동물세포를 사용해서 외래유전자 유래 단백질을 대량으로 생산하기 위한 발현 벡터, 및 그의 이용 Expired - Fee Related KR101604502B1 (ko)

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JPJP-P-2008-046782 2008-02-27
JP2008046782 2008-02-27

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KR20100135755A KR20100135755A (ko) 2010-12-27
KR101604502B1 true KR101604502B1 (ko) 2016-03-17

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KR1020107021057A Expired - Fee Related KR101604502B1 (ko) 2008-02-27 2009-02-27 동물세포를 사용해서 외래유전자 유래 단백질을 대량으로 생산하기 위한 발현 벡터, 및 그의 이용

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US (1) US8653249B2 (https=)
EP (1) EP2258843B1 (https=)
JP (2) JP5648912B2 (https=)
KR (1) KR101604502B1 (https=)
CN (1) CN102016025B (https=)
AU (1) AU2009218069B2 (https=)
CA (1) CA2716766A1 (https=)
DK (1) DK2258843T3 (https=)
ES (1) ES2616021T3 (https=)
PT (1) PT2258843T (https=)
WO (1) WO2009107775A1 (https=)

Families Citing this family (5)

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DK2380979T3 (da) 2008-12-22 2015-06-22 Univ Hokkaido Nat Univ Corp Proteinstof med trippelhelixstruktur samt fremgangsmåde til dets fremstilling
CN102325881B (zh) * 2008-12-22 2014-08-20 国立大学法人北海道大学 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用
WO2016130583A1 (en) * 2015-02-13 2016-08-18 The Board Of Trustees Of The Leland Stanford Junior University Non-silencing selectable marker genes and methods of use
JP7416355B2 (ja) * 2021-03-15 2024-01-17 株式会社ジー・キューブ 選択マーカー発現カセット
CN113969284B (zh) * 2021-11-23 2022-07-12 江南大学 一种cho细胞基因nw_003614889.1内稳定表达蛋白质的位点及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004070030A1 (ja) 2003-02-03 2004-08-19 Immuno Japan Inc. 動物細胞用高発現ベクター

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU572108B2 (en) 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein
NZ210501A (en) 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
JP2648860B2 (ja) 1986-05-08 1997-09-03 イエダ リサーチ アンド デベロップメント コンパニー リミテッド ヒトインターフエロン−β２Ａ及びヒトインターフエロン−β２Ｂ、該インターフエロンをコードする遺伝子を含むベクター、該インターフエロンを産生するセルライン及び該インターフエロンの医薬品としての用途
DE4142077A1 (de) 1991-12-19 1993-06-24 Boehringer Mannheim Gmbh Verfahren zur expression rekombinanter antikoerper
JPH0630788A (ja) 1992-07-16 1994-02-08 Otsuka Pharmaceut Co Ltd ヒトインターロイキン−１に対する組換え抗体
JP3919830B2 (ja) 1992-11-28 2007-05-30 財団法人化学及血清療法研究所 抗ネコヘルペスウイルス−１組換え抗体および該抗体をコードする遺伝子断片
JP3582965B2 (ja) 1996-08-23 2004-10-27 株式会社イムノ・ジャパン 哺乳動物細胞用発現ベクター
ES2384391T3 (es) * 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
CN100381573C (zh) * 2005-04-14 2008-04-16 北京天广实生物技术有限公司 快速构建目标基因高表达的哺乳动物细胞株的体系和方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004070030A1 (ja) 2003-02-03 2004-08-19 Immuno Japan Inc. 動物細胞用高発現ベクター

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WO2009107775A1 (ja) 2009-09-03
CA2716766A1 (en) 2009-09-03
JPWO2009107775A1 (ja) 2011-07-07
EP2258843B1 (en) 2016-11-16
AU2009218069B2 (en) 2014-05-01
KR20100135755A (ko) 2010-12-27
AU2009218069A1 (en) 2009-09-03
EP2258843A4 (en) 2012-03-14
EP2258843A1 (en) 2010-12-08
CN102016025B (zh) 2015-09-16
CN102016025A (zh) 2011-04-13
DK2258843T3 (en) 2017-02-13
JP5648912B2 (ja) 2015-01-07
US20110123993A1 (en) 2011-05-26
JP6022518B2 (ja) 2016-11-09
ES2616021T3 (es) 2017-06-09
HK1155772A1 (en) 2012-05-25
AU2009218069A2 (en) 2010-11-04
PT2258843T (pt) 2017-02-07
US8653249B2 (en) 2014-02-18
JP2015037412A (ja) 2015-02-26

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