JP5648912B2 - 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 - Google Patents

動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 Download PDF

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JP5648912B2
JP5648912B2 JP2010500766A JP2010500766A JP5648912B2 JP 5648912 B2 JP5648912 B2 JP 5648912B2 JP 2010500766 A JP2010500766 A JP 2010500766A JP 2010500766 A JP2010500766 A JP 2010500766A JP 5648912 B2 JP5648912 B2 JP 5648912B2
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gene
expression vector
hmbl
foreign gene
medium
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Japanese (ja)
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JPWO2009107775A1 (ja
Inventor
定彦 鈴木
定彦 鈴木
啓一 山本
啓一 山本
寛 田原
寛 田原
祐介 鈴木
祐介 鈴木
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Hokkaido University NUC
Fuso Pharmaceutical Industries Ltd
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Hokkaido University NUC
Fuso Pharmaceutical Industries Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
    • C12N2840/102—Vectors comprising a special translation-regulating system regulates levels of translation inhibiting translation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010500766A 2008-02-27 2009-02-27 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 Expired - Fee Related JP5648912B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2010500766A JP5648912B2 (ja) 2008-02-27 2009-02-27 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2008046782 2008-02-27
JP2008046782 2008-02-27
JP2010500766A JP5648912B2 (ja) 2008-02-27 2009-02-27 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
PCT/JP2009/053682 WO2009107775A1 (ja) 2008-02-27 2009-02-27 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用

Related Child Applications (1)

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JP2014195515A Division JP6022518B2 (ja) 2008-02-27 2014-09-25 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用

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JPWO2009107775A1 JPWO2009107775A1 (ja) 2011-07-07
JP5648912B2 true JP5648912B2 (ja) 2015-01-07

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JP2010500766A Expired - Fee Related JP5648912B2 (ja) 2008-02-27 2009-02-27 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
JP2014195515A Expired - Fee Related JP6022518B2 (ja) 2008-02-27 2014-09-25 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用

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Country Status (11)

Country Link
US (1) US8653249B2 (https=)
EP (1) EP2258843B1 (https=)
JP (2) JP5648912B2 (https=)
KR (1) KR101604502B1 (https=)
CN (1) CN102016025B (https=)
AU (1) AU2009218069B2 (https=)
CA (1) CA2716766A1 (https=)
DK (1) DK2258843T3 (https=)
ES (1) ES2616021T3 (https=)
PT (1) PT2258843T (https=)
WO (1) WO2009107775A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2380979T3 (da) 2008-12-22 2015-06-22 Univ Hokkaido Nat Univ Corp Proteinstof med trippelhelixstruktur samt fremgangsmåde til dets fremstilling
CN102325881B (zh) * 2008-12-22 2014-08-20 国立大学法人北海道大学 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用
WO2016130583A1 (en) * 2015-02-13 2016-08-18 The Board Of Trustees Of The Leland Stanford Junior University Non-silencing selectable marker genes and methods of use
JP7416355B2 (ja) * 2021-03-15 2024-01-17 株式会社ジー・キューブ 選択マーカー発現カセット
CN113969284B (zh) * 2021-11-23 2022-07-12 江南大学 一种cho细胞基因nw_003614889.1内稳定表达蛋白质的位点及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10179169A (ja) * 1996-08-23 1998-07-07 Immuno Japan:Kk 哺乳動物細胞用発現ベクター
WO2004070030A1 (ja) * 2003-02-03 2004-08-19 Immuno Japan Inc. 動物細胞用高発現ベクター

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU572108B2 (en) 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein
NZ210501A (en) 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
JP2648860B2 (ja) 1986-05-08 1997-09-03 イエダ リサーチ アンド デベロップメント コンパニー リミテッド ヒトインターフエロン−β２Ａ及びヒトインターフエロン−β２Ｂ、該インターフエロンをコードする遺伝子を含むベクター、該インターフエロンを産生するセルライン及び該インターフエロンの医薬品としての用途
DE4142077A1 (de) 1991-12-19 1993-06-24 Boehringer Mannheim Gmbh Verfahren zur expression rekombinanter antikoerper
JPH0630788A (ja) 1992-07-16 1994-02-08 Otsuka Pharmaceut Co Ltd ヒトインターロイキン−１に対する組換え抗体
JP3919830B2 (ja) 1992-11-28 2007-05-30 財団法人化学及血清療法研究所 抗ネコヘルペスウイルス−１組換え抗体および該抗体をコードする遺伝子断片
ES2384391T3 (es) * 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
CN100381573C (zh) * 2005-04-14 2008-04-16 北京天广实生物技术有限公司 快速构建目标基因高表达的哺乳动物细胞株的体系和方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10179169A (ja) * 1996-08-23 1998-07-07 Immuno Japan:Kk 哺乳動物細胞用発現ベクター
WO2004070030A1 (ja) * 2003-02-03 2004-08-19 Immuno Japan Inc. 動物細胞用高発現ベクター

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6013056088; Ｇｅｎｅ，　１９９７，　Ｖｏｌ．１９９，　ｐ．２９３-３０１ *
JPN6013056091; 許南浩，　細胞培養なるほどＱ＆Ａ，　羊土社，　２００４，　ｐ．９５-９６ *

Also Published As

Publication number Publication date
WO2009107775A1 (ja) 2009-09-03
CA2716766A1 (en) 2009-09-03
JPWO2009107775A1 (ja) 2011-07-07
KR101604502B1 (ko) 2016-03-17
EP2258843B1 (en) 2016-11-16
AU2009218069B2 (en) 2014-05-01
KR20100135755A (ko) 2010-12-27
AU2009218069A1 (en) 2009-09-03
EP2258843A4 (en) 2012-03-14
EP2258843A1 (en) 2010-12-08
CN102016025B (zh) 2015-09-16
CN102016025A (zh) 2011-04-13
DK2258843T3 (en) 2017-02-13
US20110123993A1 (en) 2011-05-26
JP6022518B2 (ja) 2016-11-09
ES2616021T3 (es) 2017-06-09
HK1155772A1 (en) 2012-05-25
AU2009218069A2 (en) 2010-11-04
PT2258843T (pt) 2017-02-07
US8653249B2 (en) 2014-02-18
JP2015037412A (ja) 2015-02-26

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