DK2258843T3 - Expression vector with mass production of foreign gene-derived protein by animal cells and use thereof - Google Patents
Expression vector with mass production of foreign gene-derived protein by animal cells and use thereof Download PDFInfo
- Publication number
- DK2258843T3 DK2258843T3 DK09715053.6T DK09715053T DK2258843T3 DK 2258843 T3 DK2258843 T3 DK 2258843T3 DK 09715053 T DK09715053 T DK 09715053T DK 2258843 T3 DK2258843 T3 DK 2258843T3
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- DK
- Denmark
- Prior art keywords
- gene
- expression vector
- hmbl
- foreign gene
- cells
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
- C12N2840/102—Vectors comprising a special translation-regulating system regulates levels of translation inhibiting translation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008046782 | 2008-02-27 | ||
| PCT/JP2009/053682 WO2009107775A1 (ja) | 2008-02-27 | 2009-02-27 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2258843T3 true DK2258843T3 (en) | 2017-02-13 |
Family
ID=41016160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09715053.6T DK2258843T3 (en) | 2008-02-27 | 2009-02-27 | Expression vector with mass production of foreign gene-derived protein by animal cells and use thereof |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8653249B2 (https=) |
| EP (1) | EP2258843B1 (https=) |
| JP (2) | JP5648912B2 (https=) |
| KR (1) | KR101604502B1 (https=) |
| CN (1) | CN102016025B (https=) |
| AU (1) | AU2009218069B2 (https=) |
| CA (1) | CA2716766A1 (https=) |
| DK (1) | DK2258843T3 (https=) |
| ES (1) | ES2616021T3 (https=) |
| PT (1) | PT2258843T (https=) |
| WO (1) | WO2009107775A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2380979T3 (da) | 2008-12-22 | 2015-06-22 | Univ Hokkaido Nat Univ Corp | Proteinstof med trippelhelixstruktur samt fremgangsmåde til dets fremstilling |
| CN102325881B (zh) * | 2008-12-22 | 2014-08-20 | 国立大学法人北海道大学 | 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用 |
| WO2016130583A1 (en) * | 2015-02-13 | 2016-08-18 | The Board Of Trustees Of The Leland Stanford Junior University | Non-silencing selectable marker genes and methods of use |
| JP7416355B2 (ja) * | 2021-03-15 | 2024-01-17 | 株式会社ジー・キューブ | 選択マーカー発現カセット |
| CN113969284B (zh) * | 2021-11-23 | 2022-07-12 | 江南大学 | 一种cho细胞基因nw_003614889.1内稳定表达蛋白质的位点及其应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU572108B2 (en) | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
| NZ210501A (en) | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| JP2648860B2 (ja) | 1986-05-08 | 1997-09-03 | イエダ リサーチ アンド デベロップメント コンパニー リミテッド | ヒトインターフエロン−β２Ａ及びヒトインターフエロン−β２Ｂ、該インターフエロンをコードする遺伝子を含むベクター、該インターフエロンを産生するセルライン及び該インターフエロンの医薬品としての用途 |
| DE4142077A1 (de) | 1991-12-19 | 1993-06-24 | Boehringer Mannheim Gmbh | Verfahren zur expression rekombinanter antikoerper |
| JPH0630788A (ja) | 1992-07-16 | 1994-02-08 | Otsuka Pharmaceut Co Ltd | ヒトインターロイキン−１に対する組換え抗体 |
| JP3919830B2 (ja) | 1992-11-28 | 2007-05-30 | 財団法人化学及血清療法研究所 | 抗ネコヘルペスウイルス−１組換え抗体および該抗体をコードする遺伝子断片 |
| JP3582965B2 (ja) | 1996-08-23 | 2004-10-27 | 株式会社イムノ・ジャパン | 哺乳動物細胞用発現ベクター |
| WO2004070030A1 (ja) | 2003-02-03 | 2004-08-19 | Immuno Japan Inc. | 動物細胞用高発現ベクター |
| ES2384391T3 (es) * | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
| KR101271884B1 (ko) | 2004-11-08 | 2013-06-05 | 크로마제닉스 비.브이. | 고수준으로 단백질을 발현하는 숙주세포의 선택 |
| CN100381573C (zh) * | 2005-04-14 | 2008-04-16 | 北京天广实生物技术有限公司 | 快速构建目标基因高表达的哺乳动物细胞株的体系和方法 |
-
2009
- 2009-02-27 EP EP09715053.6A patent/EP2258843B1/en active Active
- 2009-02-27 US US12/918,776 patent/US8653249B2/en not_active Expired - Fee Related
- 2009-02-27 CN CN200980114565.XA patent/CN102016025B/zh not_active Expired - Fee Related
- 2009-02-27 ES ES09715053.6T patent/ES2616021T3/es active Active
- 2009-02-27 KR KR1020107021057A patent/KR101604502B1/ko not_active Expired - Fee Related
- 2009-02-27 JP JP2010500766A patent/JP5648912B2/ja not_active Expired - Fee Related
- 2009-02-27 WO PCT/JP2009/053682 patent/WO2009107775A1/ja not_active Ceased
- 2009-02-27 DK DK09715053.6T patent/DK2258843T3/en active
- 2009-02-27 AU AU2009218069A patent/AU2009218069B2/en not_active Ceased
- 2009-02-27 PT PT97150536T patent/PT2258843T/pt unknown
- 2009-02-27 CA CA2716766A patent/CA2716766A1/en not_active Abandoned
-
2014
- 2014-09-25 JP JP2014195515A patent/JP6022518B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009107775A1 (ja) | 2009-09-03 |
| CA2716766A1 (en) | 2009-09-03 |
| JPWO2009107775A1 (ja) | 2011-07-07 |
| KR101604502B1 (ko) | 2016-03-17 |
| EP2258843B1 (en) | 2016-11-16 |
| AU2009218069B2 (en) | 2014-05-01 |
| KR20100135755A (ko) | 2010-12-27 |
| AU2009218069A1 (en) | 2009-09-03 |
| EP2258843A4 (en) | 2012-03-14 |
| EP2258843A1 (en) | 2010-12-08 |
| CN102016025B (zh) | 2015-09-16 |
| CN102016025A (zh) | 2011-04-13 |
| JP5648912B2 (ja) | 2015-01-07 |
| US20110123993A1 (en) | 2011-05-26 |
| JP6022518B2 (ja) | 2016-11-09 |
| ES2616021T3 (es) | 2017-06-09 |
| HK1155772A1 (en) | 2012-05-25 |
| AU2009218069A2 (en) | 2010-11-04 |
| PT2258843T (pt) | 2017-02-07 |
| US8653249B2 (en) | 2014-02-18 |
| JP2015037412A (ja) | 2015-02-26 |
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