KR101376013B1 - 기관 및 조직의 세포제거 및 재세포화 - Google Patents
기관 및 조직의 세포제거 및 재세포화 Download PDFInfo
- Publication number
- KR101376013B1 KR101376013B1 KR1020087007151A KR20087007151A KR101376013B1 KR 101376013 B1 KR101376013 B1 KR 101376013B1 KR 1020087007151 A KR1020087007151 A KR 1020087007151A KR 20087007151 A KR20087007151 A KR 20087007151A KR 101376013 B1 KR101376013 B1 KR 101376013B1
- Authority
- KR
- South Korea
- Prior art keywords
- organ
- cells
- heart
- cell
- decellularized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000056 organ Anatomy 0.000 title claims abstract description 211
- 210000004027 cell Anatomy 0.000 claims abstract description 242
- 230000010412 perfusion Effects 0.000 claims abstract description 97
- 230000001172 regenerating effect Effects 0.000 claims abstract description 58
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims abstract description 57
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims abstract description 57
- 210000002744 extracellular matrix Anatomy 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 51
- 239000007787 solid Substances 0.000 claims abstract description 35
- 210000002216 heart Anatomy 0.000 claims description 147
- 210000001519 tissue Anatomy 0.000 claims description 141
- 210000000130 stem cell Anatomy 0.000 claims description 41
- 229920002113 octoxynol Polymers 0.000 claims description 26
- 239000003599 detergent Substances 0.000 claims description 25
- 210000004204 blood vessel Anatomy 0.000 claims description 21
- 210000003734 kidney Anatomy 0.000 claims description 19
- 230000002792 vascular Effects 0.000 claims description 19
- 230000000747 cardiac effect Effects 0.000 claims description 16
- 210000004185 liver Anatomy 0.000 claims description 16
- 210000004072 lung Anatomy 0.000 claims description 14
- 241000282414 Homo sapiens Species 0.000 claims description 13
- 210000001147 pulmonary artery Anatomy 0.000 claims description 13
- 241000283690 Bos taurus Species 0.000 claims description 12
- 241001494479 Pecora Species 0.000 claims description 11
- 210000004351 coronary vessel Anatomy 0.000 claims description 11
- 241000282472 Canis lupus familiaris Species 0.000 claims description 10
- 238000003780 insertion Methods 0.000 claims description 10
- 230000037431 insertion Effects 0.000 claims description 10
- 210000005240 left ventricle Anatomy 0.000 claims description 8
- 210000002027 skeletal muscle Anatomy 0.000 claims description 8
- 210000004556 brain Anatomy 0.000 claims description 7
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 7
- 210000005246 left atrium Anatomy 0.000 claims description 7
- 210000000496 pancreas Anatomy 0.000 claims description 7
- 210000005245 right atrium Anatomy 0.000 claims description 7
- 210000000952 spleen Anatomy 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 230000004069 differentiation Effects 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 6
- 210000003492 pulmonary vein Anatomy 0.000 claims description 6
- 210000001765 aortic valve Anatomy 0.000 claims description 5
- 210000000988 bone and bone Anatomy 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 claims description 5
- 210000002403 aortic endothelial cell Anatomy 0.000 claims description 4
- 210000001054 cardiac fibroblast Anatomy 0.000 claims description 4
- 210000002429 large intestine Anatomy 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 210000005241 right ventricle Anatomy 0.000 claims description 4
- 210000000813 small intestine Anatomy 0.000 claims description 4
- 210000002784 stomach Anatomy 0.000 claims description 4
- 210000003932 urinary bladder Anatomy 0.000 claims description 4
- 239000003102 growth factor Substances 0.000 claims description 3
- 210000004115 mitral valve Anatomy 0.000 claims description 3
- 230000035755 proliferation Effects 0.000 claims description 3
- 230000015271 coagulation Effects 0.000 claims description 2
- 238000005345 coagulation Methods 0.000 claims description 2
- 239000002955 immunomodulating agent Substances 0.000 claims description 2
- 229940121354 immunomodulator Drugs 0.000 claims description 2
- 210000000591 tricuspid valve Anatomy 0.000 claims description 2
- 241000282898 Sus scrofa Species 0.000 claims 9
- 210000002798 bone marrow cell Anatomy 0.000 claims 2
- 210000004925 microvascular endothelial cell Anatomy 0.000 claims 2
- 210000004976 peripheral blood cell Anatomy 0.000 claims 2
- 210000003954 umbilical cord Anatomy 0.000 claims 2
- 230000037020 contractile activity Effects 0.000 claims 1
- 230000001434 glomerular Effects 0.000 claims 1
- 230000002584 immunomodulator Effects 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 13
- 238000002054 transplantation Methods 0.000 abstract description 4
- 239000002609 medium Substances 0.000 description 39
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 29
- 229940083575 sodium dodecyl sulfate Drugs 0.000 description 29
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 29
- 241000700159 Rattus Species 0.000 description 25
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 15
- 230000002861 ventricular Effects 0.000 description 15
- 241001465754 Metazoa Species 0.000 description 13
- 210000002469 basement membrane Anatomy 0.000 description 13
- 239000000835 fiber Substances 0.000 description 13
- 239000008367 deionised water Substances 0.000 description 12
- 229910021641 deionized water Inorganic materials 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- 210000000709 aorta Anatomy 0.000 description 10
- 229920001223 polyethylene glycol Polymers 0.000 description 10
- 229960005322 streptomycin Drugs 0.000 description 10
- 210000003462 vein Anatomy 0.000 description 10
- APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 9
- 102000009123 Fibrin Human genes 0.000 description 9
- 108010073385 Fibrin Proteins 0.000 description 9
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 9
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 9
- 229960003942 amphotericin b Drugs 0.000 description 9
- 230000008602 contraction Effects 0.000 description 9
- 210000002889 endothelial cell Anatomy 0.000 description 9
- 229950003499 fibrin Drugs 0.000 description 9
- 210000005003 heart tissue Anatomy 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 210000001185 bone marrow Anatomy 0.000 description 8
- 239000000499 gel Substances 0.000 description 8
- 230000003134 recirculating effect Effects 0.000 description 8
- 230000000638 stimulation Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 7
- 230000003115 biocidal effect Effects 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 210000001736 capillary Anatomy 0.000 description 7
- 229920000669 heparin Polymers 0.000 description 7
- 229960002897 heparin Drugs 0.000 description 7
- 230000033001 locomotion Effects 0.000 description 7
- 230000002107 myocardial effect Effects 0.000 description 7
- 238000010186 staining Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 210000004165 myocardium Anatomy 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 5
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000012981 Hank's balanced salt solution Substances 0.000 description 5
- 229930182555 Penicillin Natural products 0.000 description 5
- 102000004142 Trypsin Human genes 0.000 description 5
- 108090000631 Trypsin Proteins 0.000 description 5
- 210000001015 abdomen Anatomy 0.000 description 5
- 210000002376 aorta thoracic Anatomy 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 5
- 230000017531 blood circulation Effects 0.000 description 5
- 238000003125 immunofluorescent labeling Methods 0.000 description 5
- 210000004940 nucleus Anatomy 0.000 description 5
- 229940049954 penicillin Drugs 0.000 description 5
- 229940056360 penicillin g Drugs 0.000 description 5
- 238000011268 retreatment Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 230000002269 spontaneous effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000012588 trypsin Substances 0.000 description 5
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 230000003187 abdominal effect Effects 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 229960003699 evans blue Drugs 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000008520 organization Effects 0.000 description 4
- 230000000541 pulsatile effect Effects 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 102000013602 Cardiac Myosins Human genes 0.000 description 3
- 108010051609 Cardiac Myosins Proteins 0.000 description 3
- 102000012422 Collagen Type I Human genes 0.000 description 3
- 108010022452 Collagen Type I Proteins 0.000 description 3
- 102000016942 Elastin Human genes 0.000 description 3
- 108010014258 Elastin Proteins 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 229960000583 acetic acid Drugs 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 210000001367 artery Anatomy 0.000 description 3
- 230000000740 bleeding effect Effects 0.000 description 3
- 210000000621 bronchi Anatomy 0.000 description 3
- 230000003833 cell viability Effects 0.000 description 3
- 210000000038 chest Anatomy 0.000 description 3
- 210000003748 coronary sinus Anatomy 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 238000001493 electron microscopy Methods 0.000 description 3
- 230000003511 endothelial effect Effects 0.000 description 3
- 239000012362 glacial acetic acid Substances 0.000 description 3
- 210000002064 heart cell Anatomy 0.000 description 3
- 239000007943 implant Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000001394 metastastic effect Effects 0.000 description 3
- 206010061289 metastatic neoplasm Diseases 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 210000002254 renal artery Anatomy 0.000 description 3
- 238000001878 scanning electron micrograph Methods 0.000 description 3
- 238000004626 scanning electron microscopy Methods 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 210000005166 vasculature Anatomy 0.000 description 3
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 102000016359 Fibronectins Human genes 0.000 description 2
- 108010067306 Fibronectins Proteins 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 229930182816 L-glutamine Natural products 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 229960005305 adenosine Drugs 0.000 description 2
- 239000000908 ammonium hydroxide Substances 0.000 description 2
- 229940043379 ammonium hydroxide Drugs 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000843 anti-fungal effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 229940121375 antifungal agent Drugs 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 210000000702 aorta abdominal Anatomy 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000000013 bile duct Anatomy 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 229910002091 carbon monoxide Inorganic materials 0.000 description 2
- 210000001168 carotid artery common Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 210000002253 embryonic cardiomyocyte Anatomy 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 210000002767 hepatic artery Anatomy 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 210000000231 kidney cortex Anatomy 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000001114 myogenic effect Effects 0.000 description 2
- 210000001087 myotubule Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- -1 one or more DNases Proteins 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 210000003240 portal vein Anatomy 0.000 description 2
- 230000036316 preload Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000009163 spontaneous depolarization Effects 0.000 description 2
- 210000003270 subclavian artery Anatomy 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 108010047303 von Willebrand Factor Proteins 0.000 description 2
- 102100036537 von Willebrand factor Human genes 0.000 description 2
- 229960001134 von willebrand factor Drugs 0.000 description 2
- PCGISRHGYLRXSR-UHFFFAOYSA-N 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)carbamoylamino]naphthalene-2-sulfonic acid Chemical compound OC1=CC(S(O)(=O)=O)=CC2=CC(NC(=O)NC=3C=C4C=C(C=C(C4=CC=3)O)S(O)(=O)=O)=CC=C21 PCGISRHGYLRXSR-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
- 102100030009 Azurocidin Human genes 0.000 description 1
- 101710154607 Azurocidin Proteins 0.000 description 1
- 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 description 1
- 102100028728 Bone morphogenetic protein 1 Human genes 0.000 description 1
- 108090000654 Bone morphogenetic protein 1 Proteins 0.000 description 1
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001631457 Cannula Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102100030074 Dickkopf-related protein 1 Human genes 0.000 description 1
- 101710099518 Dickkopf-related protein 1 Proteins 0.000 description 1
- 206010013012 Dilatation ventricular Diseases 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- 101150021185 FGF gene Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 241000288105 Grus Species 0.000 description 1
- 102100031547 HLA class II histocompatibility antigen, DO alpha chain Human genes 0.000 description 1
- 102100021866 Hepatocyte growth factor Human genes 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000866278 Homo sapiens HLA class II histocompatibility antigen, DO alpha chain Proteins 0.000 description 1
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 description 1
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 1
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 description 1
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 description 1
- 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 description 1
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 1
- 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 description 1
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 101001055320 Myxine glutinosa Insulin-like growth factor Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
- 108010067787 Proteoglycans Proteins 0.000 description 1
- 101000933967 Pseudomonas phage KPP25 Major capsid protein Proteins 0.000 description 1
- 108010081750 Reticulin Proteins 0.000 description 1
- 206010040744 Sinus headache Diseases 0.000 description 1
- 102100021669 Stromal cell-derived factor 1 Human genes 0.000 description 1
- 101710088580 Stromal cell-derived factor 1 Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 102000002938 Thrombospondin Human genes 0.000 description 1
- 108060008245 Thrombospondin Proteins 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 229960001138 acetylsalicylic acid Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 230000002491 angiogenic effect Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000001028 anti-proliverative effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 201000002064 aortic valve insufficiency Diseases 0.000 description 1
- 230000008321 arterial blood flow Effects 0.000 description 1
- 230000004872 arterial blood pressure Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000003143 atherosclerotic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 1
- 210000002665 bowman capsule Anatomy 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000010366 cell biology technique Methods 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000002442 collagenase inhibitor Substances 0.000 description 1
- 210000000555 contractile cell Anatomy 0.000 description 1
- 230000009989 contractile response Effects 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 108010007093 dispase Proteins 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 210000003038 endothelium Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029578 entry into host Effects 0.000 description 1
- 210000003238 esophagus Anatomy 0.000 description 1
- 210000003414 extremity Anatomy 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 210000002468 fat body Anatomy 0.000 description 1
- 210000003191 femoral vein Anatomy 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 210000000604 fetal stem cell Anatomy 0.000 description 1
- 230000002332 fibrillatory effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000005086 glomerual capillary Anatomy 0.000 description 1
- 210000000585 glomerular basement membrane Anatomy 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000037183 heart physiology Effects 0.000 description 1
- 210000002989 hepatic vein Anatomy 0.000 description 1
- 210000004394 hip joint Anatomy 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000010324 immunological assay Methods 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000013383 initial experiment Methods 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 230000037427 ion transport Effects 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 229960002725 isoflurane Drugs 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 238000002350 laparotomy Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 230000036723 left ventricular dilatation Effects 0.000 description 1
- 239000003199 leukotriene receptor blocking agent Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000012120 mounting media Substances 0.000 description 1
- 210000002894 multi-fate stem cell Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000003098 myoblast Anatomy 0.000 description 1
- 210000000107 myocyte Anatomy 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000007826 nucleic acid assay Methods 0.000 description 1
- 229910000489 osmium tetroxide Inorganic materials 0.000 description 1
- 239000012285 osmium tetroxide Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 210000001711 oxyntic cell Anatomy 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000002831 pharmacologic agent Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920002523 polyethylene Glycol 1000 Polymers 0.000 description 1
- 229940113116 polyethylene glycol 1000 Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 210000003102 pulmonary valve Anatomy 0.000 description 1
- 238000000718 qrs complex Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000002336 repolarization Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 210000001533 respiratory mucosa Anatomy 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 210000000323 shoulder joint Anatomy 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 210000004683 skeletal myoblast Anatomy 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
- 238000000352 supercritical drying Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 229940037128 systemic glucocorticoids Drugs 0.000 description 1
- 210000001042 thoracic artery Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 210000001631 vena cava inferior Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M60/00—Blood pumps; Devices for mechanical circulatory actuation; Balloon pumps for circulatory assistance
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0024—Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Sustainable Development (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Cardiology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Heart & Thoracic Surgery (AREA)
- Rheumatology (AREA)
- Neurosurgery (AREA)
- Dermatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Vascular Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Mechanical Engineering (AREA)
- Anesthesiology (AREA)
- Hematology (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71150105P | 2005-08-26 | 2005-08-26 | |
| US60/711,501 | 2005-08-26 | ||
| US81524206P | 2006-06-19 | 2006-06-19 | |
| US60/815,242 | 2006-06-19 | ||
| PCT/US2006/033415 WO2007025233A1 (en) | 2005-08-26 | 2006-08-28 | Decellularization and recellularization of organs and tissues |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020137028378A Division KR101420585B1 (ko) | 2005-08-26 | 2006-08-28 | 기관 및 조직의 세포제거 및 재세포화 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20080065970A KR20080065970A (ko) | 2008-07-15 |
| KR101376013B1 true KR101376013B1 (ko) | 2014-03-19 |
Family
ID=37771950
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020137028378A Active KR101420585B1 (ko) | 2005-08-26 | 2006-08-28 | 기관 및 조직의 세포제거 및 재세포화 |
| KR1020147007674A Ceased KR20140040293A (ko) | 2005-08-26 | 2006-08-28 | 기관 및 조직의 세포제거 및 재세포화 |
| KR1020087007151A Active KR101376013B1 (ko) | 2005-08-26 | 2006-08-28 | 기관 및 조직의 세포제거 및 재세포화 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020137028378A Active KR101420585B1 (ko) | 2005-08-26 | 2006-08-28 | 기관 및 조직의 세포제거 및 재세포화 |
| KR1020147007674A Ceased KR20140040293A (ko) | 2005-08-26 | 2006-08-28 | 기관 및 조직의 세포제거 및 재세포화 |
Country Status (15)
| Country | Link |
|---|---|
| US (5) | US8470520B2 (enExample) |
| EP (3) | EP2431063B1 (enExample) |
| JP (6) | JP2009505752A (enExample) |
| KR (3) | KR101420585B1 (enExample) |
| CN (2) | CN101272815B (enExample) |
| AT (1) | ATE552007T1 (enExample) |
| AU (1) | AU2006282783B2 (enExample) |
| CA (1) | CA2618731C (enExample) |
| ES (2) | ES2384721T3 (enExample) |
| IL (2) | IL189418A (enExample) |
| MX (3) | MX2008002589A (enExample) |
| PL (2) | PL2431063T3 (enExample) |
| RU (2) | RU2463081C2 (enExample) |
| SG (1) | SG170731A1 (enExample) |
| WO (1) | WO2007025233A1 (enExample) |
Families Citing this family (136)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100093066A1 (en) * | 2005-08-26 | 2010-04-15 | Regents Of The University Of Minnesota | Decellularization and recellularization apparatuses and systems containing the same |
| KR101420585B1 (ko) * | 2005-08-26 | 2014-07-17 | 리전츠 오브 더 유니버스티 오브 미네소타 | 기관 및 조직의 세포제거 및 재세포화 |
| JP2010528613A (ja) | 2007-05-29 | 2010-08-26 | クリストファー ビー. リード, | 多能細胞集団を産生する方法およびその使用 |
| US20090180965A1 (en) * | 2008-01-04 | 2009-07-16 | Boston Scientific Scimed, Inc. | Methods for repair and regeneration of bone marrow |
| AU2009266761A1 (en) * | 2008-07-02 | 2010-01-07 | Jichi Medical University | Artificial kidney precursor and process for production thereof |
| AU2009283516B2 (en) * | 2008-08-20 | 2014-02-13 | Organ Technologies Inc. | Method for restoring missing tooth and method for producing restorative material |
| US9192695B2 (en) | 2008-11-20 | 2015-11-24 | Allosource | Allografts combined with tissue derived stem cells for bone healing |
| CA2751133C (en) | 2009-02-04 | 2020-07-21 | Yale University | Tissue engineering of lung |
| WO2010099310A1 (en) * | 2009-02-26 | 2010-09-02 | Wake Forest University Health Sciences | Endothelial scaffolds |
| KR101029887B1 (ko) * | 2009-03-04 | 2011-04-18 | 서울대학교산학협력단 | 탈세포화를 위한 돼지 각막의 가공방법 |
| CA2757457C (en) * | 2009-03-31 | 2018-05-29 | Regents Of The University Of Minnesota | Decellularization and recellularization of organs and tissues |
| KR101753797B1 (ko) * | 2009-06-04 | 2017-07-04 | 더 제너럴 하스피탈 코포레이션 | 생인공 폐 |
| US20130323708A1 (en) | 2009-07-01 | 2013-12-05 | Massachusetts Institute Of Technology | Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same |
| WO2011005306A2 (en) * | 2009-07-07 | 2011-01-13 | The Board Of Regents Of The University Of Texas System | Production of and uses for decellularized lung tissue |
| RU2407459C1 (ru) * | 2009-07-27 | 2010-12-27 | Учреждение Российской академии медицинских наук Научно-исследовательский институт кардиологии Сибирского отделения РАМН | Способ получения соединительно-тканного каркаса магистрального сосуда млекопитающих животных и человека |
| CA2770490C (en) * | 2009-08-11 | 2024-04-16 | The Johns Hopkins University | Compositions and methods for implantation of processed adipose tissue and processed adipose tissue products |
| US20110236949A1 (en) * | 2009-09-22 | 2011-09-29 | Colorado State University Research Foundation | Methods for Processing Biological Tissues |
| US20130177972A1 (en) * | 2009-11-17 | 2013-07-11 | David Green | Bioreactors, systems, and methods for producing and/or analyzing organs |
| WO2011068778A1 (en) * | 2009-12-03 | 2011-06-09 | Lifecell Corporation | Nerve treatment devices and methods |
| US9034386B2 (en) * | 2009-12-17 | 2015-05-19 | Queen's University At Kingston | Decellularized adipose tissue |
| EP2524034A1 (en) * | 2010-01-14 | 2012-11-21 | Organogenesis, Inc. | Bioengineered tissue constructs and methods for producing and using thereof |
| WO2011140241A2 (en) | 2010-05-04 | 2011-11-10 | The General Hospital Corporation | Methods and compositions for preserving tissues and organs |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| CN101850136B (zh) * | 2010-06-11 | 2013-01-23 | 四川大学华西医院 | 一种植入式生物人工肝脏 |
| US20120064537A1 (en) * | 2010-06-30 | 2012-03-15 | Miromatrix Medical Inc. | Use of perfusion decellularized organs for matched recellularization |
| EP2611472B1 (en) * | 2010-09-01 | 2016-02-10 | Regents of the University of Minnesota | Methods of recellularizing a tissue or organ for improved transplantability |
| KR101108983B1 (ko) * | 2010-09-17 | 2012-01-31 | 강원대학교산학협력단 | 다장기 바이오스캐폴드의 제조방법 |
| KR101273489B1 (ko) * | 2011-04-08 | 2013-06-21 | 강원대학교산학협력단 | 심폐 동시 이식용 바이오스캐폴드의 제조방법 |
| KR102068071B1 (ko) | 2011-04-28 | 2020-01-20 | 라이프셀 코포레이션 | 조직 생성물의 효소처리방법 |
| US10207025B2 (en) | 2011-04-28 | 2019-02-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
| US9238793B2 (en) | 2011-04-28 | 2016-01-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
| US8834928B1 (en) | 2011-05-16 | 2014-09-16 | Musculoskeletal Transplant Foundation | Tissue-derived tissugenic implants, and methods of fabricating and using same |
| JP6158175B2 (ja) * | 2011-05-27 | 2017-07-05 | デピュイ・シンセス・プロダクツ・インコーポレイテッド | バイオ人工近位尿細管システム及びその使用方法 |
| US20130143323A1 (en) * | 2011-06-01 | 2013-06-06 | Geoffrey S. FROST | Apparatus and method for decellularizing, recellularizing or treating organs |
| WO2012170490A2 (en) * | 2011-06-06 | 2012-12-13 | Invivosciences, Llc | Engineered cardiac tissues and methods of using them |
| WO2013028968A1 (en) * | 2011-08-25 | 2013-02-28 | Wisconsin Alumni Research Foundation | 3-dimensional cardiac fibroblast derived extracellular matrix |
| WO2013052138A2 (en) * | 2011-10-03 | 2013-04-11 | Fabrico. Inc. | System and method for electrophoretic decellularization |
| KR101360226B1 (ko) * | 2011-11-04 | 2014-02-12 | 강원대학교산학협력단 | 심폐 일체형 탈세포화기법 |
| EP2775957A4 (en) * | 2011-11-09 | 2015-07-22 | Gen Hospital Corp | COMPOUND TISSUE TRANSPLANT, MATERIALS AND METHOD FOR THE PRODUCTION AND USE THEREOF |
| EP3822340A1 (en) * | 2012-01-13 | 2021-05-19 | The General Hospital Corporation | Isolated human lung progenitor cells and uses thereof |
| EP2809152B1 (en) | 2012-02-01 | 2019-11-20 | Nayacure Therapeutics Ltd | Method for inducing immune tolerance to organ transplants |
| PT2782995T (pt) * | 2012-03-16 | 2017-02-14 | Novahep Ab | Vaso sanguíneo alogénico obtido mediante bioengenharia |
| WO2013158283A1 (en) * | 2012-04-18 | 2013-10-24 | The General Hospital Corporation | Bioartificial filtration organ |
| US9877822B2 (en) | 2012-04-24 | 2018-01-30 | Biostage, Inc. | Engineered tissue scaffolds and supports therefor |
| US20130344490A1 (en) * | 2012-04-27 | 2013-12-26 | Min Peter Kim | Neoplastic cells grown on decellularized biomatrix |
| US9290738B2 (en) * | 2012-06-13 | 2016-03-22 | Miromatrix Medical Inc. | Methods of decellularizing bone |
| GB201212771D0 (en) * | 2012-07-18 | 2012-08-29 | Northwick Park Inst For Medcal Res Ltd | Implant and method for producing an implant |
| US9297806B2 (en) * | 2012-08-01 | 2016-03-29 | The Johns Hopkins University | 5-hydroxymethylcytosine in human cancer |
| US10111740B2 (en) | 2012-08-21 | 2018-10-30 | Regents Of The University Of Minnesota | Decellularized biologically-engineered tubular grafts |
| US9232804B2 (en) | 2012-10-11 | 2016-01-12 | Knu University-Industry Cooperation Foundation | Compositions and methods for sterilizing bioscaffolds |
| CA2890661A1 (en) * | 2012-11-07 | 2014-07-10 | James Bennie Gandy | Tissue transplantation involving, and tissues modified by decellularization and recellularization of donor tissues for minimized or obviated rejection reactions |
| US11284987B2 (en) | 2013-01-09 | 2022-03-29 | Biostage, Inc. | Synthetic scaffolds |
| WO2014110586A1 (en) * | 2013-01-14 | 2014-07-17 | Florida State University Research Foundation, Inc. | Extracellular matrix derived from stem cells and methods for production |
| CN103127550A (zh) * | 2013-02-26 | 2013-06-05 | 中国人民解放军第二军医大学 | 骨骼肌全器官脱细胞基质、制备方法及其衍生出的医疗产品 |
| CA2897855A1 (en) | 2013-03-07 | 2014-09-12 | Allosource | Consistent calcium content bone allograft systems and methods |
| KR20230061578A (ko) | 2013-03-15 | 2023-05-08 | 미로매트릭스 메디칼 인크. | 섬 세포 재세포화를 위한 관류 탈세포화된 간의 용도 |
| US9888680B2 (en) * | 2013-04-04 | 2018-02-13 | The Trustees Of Columbia University In The City Of New York | Functional recovery of human lungs for transplantation |
| EP2983718A4 (en) * | 2013-04-11 | 2016-12-07 | Univ Of Vermont And State Agricultural College | Decellularization and recellularization of whole organs |
| CA2909267C (en) * | 2013-04-12 | 2022-10-25 | Saverio LA FRANCESCA | Improving organs for transplantation |
| GB201310773D0 (en) * | 2013-06-17 | 2013-07-31 | Northwick Park Inst For Medcal Res Ltd | Implant and method of producing an implant |
| AU2014296259B2 (en) | 2013-07-30 | 2017-04-27 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| KR101636954B1 (ko) | 2013-11-04 | 2016-07-08 | 대한민국 | 재세포화된 바이오 스캐폴드 및 이의 제조방법 |
| BR112016009178B1 (pt) | 2013-11-04 | 2020-12-15 | Lifecell Corporation | Método de preparação de um produto de tecido e uso de um produto de tecido |
| WO2015081226A1 (en) | 2013-11-27 | 2015-06-04 | The Johns Hopkins University | Engineered cardiac derived compositions and methods of use |
| CN103736150B (zh) * | 2013-12-31 | 2015-11-18 | 中山大学 | 褪黑素协同细胞外基质生物材料在制备促进间充质干细胞成骨分化药物中的应用 |
| CN103750905B (zh) * | 2014-01-20 | 2015-08-12 | 辽宁中医药大学 | 大动物离体工作心双通道灌流实验方法及设备 |
| US11648335B2 (en) | 2014-01-31 | 2023-05-16 | Wake Forest University Health Sciences | Organ/tissue decellularization, framework maintenance and recellularization |
| JP6271298B2 (ja) * | 2014-02-28 | 2018-01-31 | 国立大学法人 東京医科歯科大学 | 脱細胞組織の製造方法 |
| JP6271299B2 (ja) * | 2014-02-28 | 2018-01-31 | 株式会社Adeka | 脱細胞組織の製造方法 |
| WO2015138832A1 (en) | 2014-03-13 | 2015-09-17 | The General Hospital Corporation | Devices and methods to improve and assess viability of human livers |
| CN117694335A (zh) * | 2014-03-14 | 2024-03-15 | 通用医疗公司 | 肺生物反应器 |
| CN104940997A (zh) * | 2014-03-27 | 2015-09-30 | 复旦大学 | 一种组织工程化人类心肌组织 |
| JP6580329B2 (ja) | 2014-03-31 | 2019-09-25 | シスメックス株式会社 | 未分化細胞から分化細胞および/または分化細胞の産生物を取得する方法 |
| WO2015168528A1 (en) * | 2014-05-02 | 2015-11-05 | University Of Houston System | Two stage cellularization strategy for the fabrication of bioartificial hearts |
| WO2015181245A1 (en) * | 2014-05-27 | 2015-12-03 | Novahep Ab | Bioengineered allogeneic valve |
| GB201409858D0 (en) * | 2014-06-03 | 2014-07-16 | Ucl Business Plc | Human liver scaffolds |
| CN104208751A (zh) * | 2014-08-19 | 2014-12-17 | 温州医科大学 | 一种新型肾脏去细胞生物支架的制备方法 |
| CA3217634A1 (en) | 2014-09-02 | 2016-03-10 | United Therapeutics Corporation | Automated bioreactor system, system for automatically implementing protocol for decellularizing organ, and waste decontamination system |
| KR101693831B1 (ko) * | 2014-09-03 | 2017-01-17 | 서울대학교산학협력단 | 스페이스 필러를 이용한 이종이식조직의 제조방법 |
| EP3006557A1 (en) | 2014-10-07 | 2016-04-13 | Heinrich-Heine-Universität Düsseldorf | Bile acids for inducing hepatic differentiation |
| BR112017008855A2 (pt) | 2014-10-31 | 2017-12-19 | The Administrators Of The Tulane Educational Fund | enxerto cirúrgico para enxertar em um paciente, método de fabricação de um enxerto cirúrgico para substituir uma parte de corpo em um paciente, método de enxerto, composição, método de fornecimento de uma cobertura protetora para um ferimento superficial de pele, e método de descelularização substancial da epiderme |
| CN104740684A (zh) * | 2015-03-03 | 2015-07-01 | 上海市闸北区中心医院 | 一种犬子宫全器官脱细胞基质及其制备方法和应用 |
| EP3274007B1 (en) | 2015-03-26 | 2020-09-30 | Miromatrix Medical Inc. | Gas filled decellularized extracellular matrix |
| JP2016185115A (ja) | 2015-03-27 | 2016-10-27 | シスメックス株式会社 | 臓器灌流方法および臓器灌流装置 |
| WO2016187413A1 (en) | 2015-05-21 | 2016-11-24 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
| RU2586952C1 (ru) * | 2015-07-20 | 2016-06-10 | Федеральное государственное бюджетное учреждение "Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова" Министерства здравоохранения Российской Федерации | Способ лечения печеночной недостаточности |
| US10912864B2 (en) | 2015-07-24 | 2021-02-09 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| CN105013014A (zh) * | 2015-07-31 | 2015-11-04 | 武汉蓝普医品有限公司 | 一种无细胞基质生物材料的制备方法和用途 |
| CN105031731B (zh) * | 2015-08-18 | 2018-04-17 | 温州医科大学 | 一种快速去细胞单叶肝脏生物支架的制备方法 |
| US11052175B2 (en) | 2015-08-19 | 2021-07-06 | Musculoskeletal Transplant Foundation | Cartilage-derived implants and methods of making and using same |
| CN108699513B (zh) | 2015-09-11 | 2022-03-18 | 通用医疗公司 | 功能性肺血管床的再生 |
| NZ741377A (en) | 2015-10-09 | 2022-02-25 | Deka Products Lp | Fluid pumping and bioreactor system |
| EP3184128B1 (en) * | 2015-12-21 | 2019-12-11 | Heinrich-Heine-Universität Düsseldorf | Method for producing a support device |
| KR101860798B1 (ko) * | 2015-12-29 | 2018-05-25 | 포항공과대학교 산학협력단 | 뇌 조직 탈세포 방법 및 뇌 조직 배양 방법 |
| KR20180133253A (ko) | 2016-04-05 | 2018-12-13 | 리젠츠 오브 더 유니버시티 오브 미네소타 | 조직에 매립되어 있는 구조적 구성요소를 갖는 공학처리된 조직, 및 제조 및 사용하는 방법 |
| CN109689071B (zh) * | 2016-05-16 | 2023-05-30 | 通用医疗公司 | 肺上皮工程中的人气道干细胞 |
| US10624992B2 (en) | 2016-05-16 | 2020-04-21 | The General Hospital Corporation | Human airway stem cells in lung epithelial engineering |
| CA3027505A1 (en) | 2016-06-15 | 2017-12-21 | The General Hospital Corporation | Metabolic labeling and molecular enhancement of biological materials using bioorthogonal reactions |
| CN106075583B (zh) * | 2016-07-07 | 2020-07-14 | 中国人民解放军第二军医大学 | 一种采用灌注-压差法制备脱细胞基质生物材料的方法 |
| EP3484409A4 (en) | 2016-07-14 | 2020-08-19 | Qidni Labs, Inc. | BIOCOMPATIBLE AND HEMOCOMPATIBLE MATERIAL AND FILTER |
| RU2639404C1 (ru) * | 2016-08-24 | 2017-12-21 | Государственное автономное учреждение здравоохранения Свердловской области "Центр специализированных видов медицинской помощи "Институт медицинских клеточных технологий" | Способ восстановления лимфоидной ткани селезенки лабораторных животных |
| WO2018048899A1 (en) | 2016-09-06 | 2018-03-15 | Micromatrix Medical Inc. | Use of resected liver serum for whole liver engineering |
| FR3055805B1 (fr) | 2016-09-09 | 2020-05-15 | Assistance Publique - Hopitaux De Paris (Ap-Hp) | Biomateriau a usage therapeutique |
| US11299705B2 (en) | 2016-11-07 | 2022-04-12 | Deka Products Limited Partnership | System and method for creating tissue |
| JP6942191B2 (ja) | 2017-01-30 | 2021-09-29 | ライフセル コーポレーションLifeCell Corporation | トランスグルタミナーゼ処理製品 |
| WO2018140854A1 (en) | 2017-01-30 | 2018-08-02 | Lifecell Corporation | Tissue matrix materials and enzymatic adhesives |
| CN107096070B (zh) * | 2017-03-09 | 2020-08-21 | 中国医学科学院阜外医院 | 一种脱细胞肺支架及其制备方法 |
| RU2665962C1 (ru) | 2017-03-17 | 2018-09-05 | Общество с ограниченной ответственностью "Матрифлекс" | Биорезорбируемый биологический матрикс для замещения дефектов костной ткани и способ его получения |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| CN107137769A (zh) * | 2017-06-06 | 2017-09-08 | 中国人民解放军军事医学科学院基础医学研究所 | 一种心脏全器官脱细胞基质的制备方法 |
| US12058996B2 (en) | 2017-06-14 | 2024-08-13 | The General Hospital Corporation | High subzero cryopreservation |
| EP3685661A4 (en) * | 2017-09-19 | 2021-05-19 | Meiji University | PROCESS FOR DEVELOPMENT OF AN ORGAN LACKING A SPECIFIC FUNCTIONAL CELL |
| US12070530B2 (en) | 2017-11-09 | 2024-08-27 | University of Pittsburgh—of the Commonwealth System of Higher Education | Decellularization and functionalization of extracellular matrix biomaterials |
| JP7174984B2 (ja) * | 2018-02-15 | 2022-11-18 | 学校法人自治医科大学 | 脱細胞化担体製造方法、脱細胞化担体保存方法、細胞充填方法、細胞シート作製方法、及び脱細胞化溶液キット |
| GB201807788D0 (en) | 2018-05-14 | 2018-06-27 | Ucl Business Plc | Method |
| US12167729B2 (en) | 2018-05-30 | 2024-12-17 | The General Hospital Corporation | Cryopreservation of tissues and organs |
| US12263275B2 (en) | 2018-06-13 | 2025-04-01 | Miromatrix Medical Inc. | Fistula filler and deployment system |
| TWI712687B (zh) * | 2018-09-11 | 2020-12-11 | 亞果生醫股份有限公司 | 去細胞器官及其製備方法 |
| AU2020241583A1 (en) * | 2019-03-21 | 2021-10-14 | Miromatrix Medical Inc. | Improved decellularization of isolated organs |
| WO2020243298A1 (en) | 2019-05-28 | 2020-12-03 | Regents Of The University Of Minnesota | An engineered valve and method of making |
| CN110694113B (zh) * | 2019-10-15 | 2022-02-01 | 南通大学附属医院 | 一种脑脱细胞支架的制备方法 |
| US20230015009A1 (en) * | 2019-12-04 | 2023-01-19 | Miromatrix Medical Inc. | Methods of Decellularization and Recellularization of Organs and Portions of Organs |
| KR102748928B1 (ko) * | 2020-02-14 | 2025-01-02 | 주식회사 세라트젠 | 심장 오가노이드 배양 및 이식을 위한 탈세포 심장 조직 유래 지지체 및 이의 제조방법 |
| WO2021162533A1 (ko) * | 2020-02-14 | 2021-08-19 | 연세대학교 산학협력단 | 장기 오가노이드 배양 및 이식을 위한 탈세포 장기 조직 유래 지지체 및 이의 제조방법 |
| CN111494718B (zh) * | 2020-04-24 | 2021-05-14 | 四川大学华西医院 | 一种动物去细胞化肺生物支架材料的制备方法 |
| KR102473682B1 (ko) * | 2020-06-30 | 2022-12-02 | 서울대학교 산학협력단 | 개인 맞춤형 경피적 심장 반월판 스텐트 판막의 제조 방법 |
| CN112354014A (zh) * | 2020-10-20 | 2021-02-12 | 上海亚朋生物技术有限公司 | 一种清洗脱细胞试剂sds的方法 |
| CN112274697A (zh) * | 2020-11-04 | 2021-01-29 | 王寅 | 一种组织工程心肌补片制备方法 |
| US11998662B1 (en) | 2021-06-09 | 2024-06-04 | Reprise Biomedical, Inc. | Biologic matrix for a wound site and related methods |
| US12383657B1 (en) | 2021-06-09 | 2025-08-12 | Reprise Biomedical, Inc. | Biologic matrix for a wound site and related methods |
| DE102021211875B3 (de) * | 2021-10-21 | 2023-01-19 | Friedrich-Alexander-Universität Erlangen-Nürnberg, Körperschaft des öffentlichen Rechts | Bioreaktor und Verfahren zum Betrieb eines solchen |
| WO2024006673A1 (en) * | 2022-06-30 | 2024-01-04 | Regenmedix Llc | Stem cell and organ maturation system and method |
| CN115554472B (zh) * | 2022-09-22 | 2023-08-18 | 舩本诚一 | 一种用于移植的生物组织处理方法 |
| EP4619054A1 (en) | 2022-11-18 | 2025-09-24 | Vantive Health GmbH | Extracorporeal blood treatment for organ support |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6416995B1 (en) | 1999-11-22 | 2002-07-09 | Bio Science Consultants, L.L.C. | Bioreactor mediated recellularization of natural and tissue engineered vascular grafts |
| US6479064B1 (en) | 1999-12-29 | 2002-11-12 | Children's Medical Center Corporation | Culturing different cell populations on a decellularized natural biostructure for organ reconstruction |
| US20030087428A1 (en) | 1999-06-07 | 2003-05-08 | Lloyd Wolfinbarger | Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced |
| WO2007025233A1 (en) | 2005-08-26 | 2007-03-01 | Regents Of The University Of Minnesota | Decellularization and recellularization of organs and tissues |
Family Cites Families (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3545221A (en) | 1967-05-22 | 1970-12-08 | Swenko Research & Dev Inc | Apparatus for maintaining organs in vitro in a completely viable state |
| US3639084A (en) | 1970-04-06 | 1972-02-01 | Baxter Laboratories Inc | Mechanism for control pulsatile fluid flow |
| DE2453363B2 (de) | 1974-11-11 | 1976-08-26 | Solco Basel AG, Birsfelden (Schweiz) | Verfahren zur herstellung heterologer arterientransplantate |
| JPS5516016A (en) | 1978-07-18 | 1980-02-04 | Dai Ichi Kogyo Seiyaku Co Ltd | Improved salt-resistant water-absorbing material |
| US4801299A (en) | 1983-06-10 | 1989-01-31 | University Patents, Inc. | Body implants of extracellular matrix and means and methods of making and using such implants |
| US5336616A (en) | 1990-09-12 | 1994-08-09 | Lifecell Corporation | Method for processing and preserving collagen-based tissues for transplantation |
| KR970705951A (ko) | 1994-09-12 | 1997-11-03 | 가일 케이. 노우톤 | 심장 판막 구조에서 3차원 사람 세포 배양물과 이의 용도(three-dimensional human cell cultures on cardiac valve franeworks and their uses) |
| WO1998046165A1 (en) * | 1997-04-11 | 1998-10-22 | Cryolife, Inc. | Tissue decellularization |
| JPH1176400A (ja) | 1997-09-09 | 1999-03-23 | Masahito Nagaki | 人工臓器 |
| JP4222658B2 (ja) | 1998-06-23 | 2009-02-12 | テルモ株式会社 | 細胞支持基材、培養装置および液体処理装置 |
| US6448076B2 (en) | 1998-09-15 | 2002-09-10 | The Regents Of The University Of Michigan | Method for chemically acellularizing a biological tissue sample |
| US20050249816A1 (en) | 1999-12-29 | 2005-11-10 | Wake Forest University Health Services | Tissue engineered liver constructs |
| US6376244B1 (en) | 1999-12-29 | 2002-04-23 | Children's Medical Center Corporation | Methods and compositions for organ decellularization |
| CA2407328A1 (en) * | 2000-04-28 | 2001-11-08 | Emory University | Decellularized vascular prostheses |
| DE10021627B4 (de) | 2000-05-04 | 2009-11-19 | Corlife Gbr (Vertretungsberechtigte Gesellschafter: Prof. Dr. Alex Haverich | Verfahren zur Herstellung eines vaskularisierten bioartifiziellen Gewebes und zugehöriger Versuchsreaktor |
| ATE332964T1 (de) | 2000-08-16 | 2006-08-15 | Univ Duke | Mittels gewebetechnologie hergestellte dezellularisierte gegenstände und gewebe |
| US6749064B1 (en) | 2000-08-25 | 2004-06-15 | Sanita L. Alrey | Bag with article display aperture and support surface |
| WO2002024244A2 (en) | 2000-09-20 | 2002-03-28 | Regeneration Technologies, Inc. | Method of preparing and processing transplant tissue |
| IL139708A0 (en) | 2000-11-15 | 2002-02-10 | Amiel Gilad | Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering |
| DE10064948C1 (de) | 2000-12-20 | 2002-07-11 | Auto Tissue Gmbh | Verfahren zur Dezellularisierung von Fremdmaterial zur Herstellung von Bioprothesen und Vorrichtung zur Durchführung des Verfahrens |
| ES2545899T3 (es) | 2001-02-14 | 2015-09-16 | Anthrogenesis Corporation | Placenta post-parto de mamíferos, su uso y células madre placentarias de la misma |
| EP1387885A1 (en) | 2001-05-01 | 2004-02-11 | Universite Du Quebec A Montreal | Tridimensional biocompatible support structure for bioartificial organs and uses thereof |
| GB2375771A (en) | 2001-05-24 | 2002-11-27 | Univ Leeds | Decellularisation of tissue implant material |
| EP1317934A1 (de) * | 2001-11-08 | 2003-06-11 | Artiss GmbH | Verfahren zur Herstellung einer azellularisierten Gewebematrix und damit erhältliche Gewebematrix |
| EP2292278B1 (en) | 2001-11-16 | 2013-04-24 | Children's Medical Center Corporation | Augmentation of organ function |
| BR0214212A (pt) | 2001-11-16 | 2004-10-26 | Childrens Medical Center | Criação de órgãos reprodutores femininos construìdos por tecido |
| US20030180268A1 (en) | 2002-02-05 | 2003-09-25 | Anthony Atala | Tissue engineered construct for supplementing or replacing a damaged organ |
| US6790475B2 (en) | 2002-04-09 | 2004-09-14 | Wafermasters Inc. | Source gas delivery |
| JP4149897B2 (ja) | 2002-11-07 | 2008-09-17 | 学校法人 聖マリアンナ医科大学 | 気管移植片の調製方法および気管移植片 |
| US20040187877A1 (en) | 2002-12-04 | 2004-09-30 | Badylak Stephen F. | Method for repair of liver tissue |
| BR0317177A (pt) | 2002-12-13 | 2005-10-25 | Cilag Ag | Preparações de liberação controlada compreendendo tramadol e topiramato |
| US20040176855A1 (en) | 2003-03-07 | 2004-09-09 | Acell, Inc. | Decellularized liver for repair of tissue and treatment of organ deficiency |
| JP2006223101A (ja) * | 2003-05-15 | 2006-08-31 | Univ Waseda | 生体組織の保持装置及びこれを用いた生体組織処理装置 |
| WO2005118014A2 (en) * | 2004-06-01 | 2005-12-15 | Medtronic, Inc. | Decellurisation processes for making bioprotheses |
| JP3773943B2 (ja) | 2004-09-24 | 2006-05-10 | 国立大学法人 東京大学 | 把持状態判定システム及び方法 |
| DE102005023599A1 (de) | 2005-05-18 | 2006-11-23 | Corlife Gbr (Vertretungsberechtigte Gesellschafter: Prof. Dr. Alex Haverich | Bioartifizielles Herzgewebetransplantat und Verfahren zu seiner Herstellung |
| WO2006126219A1 (en) | 2005-05-26 | 2006-11-30 | Fresenius Medical Care Deutschland G.M.B.H. | Liver progenitor cells |
| US7824912B2 (en) | 2005-06-23 | 2010-11-02 | Massachusetts Institute Of Technology | Methods for ex vivo propagation of adult hepatic stem cells |
| AU2013224686B2 (en) | 2005-08-26 | 2015-06-11 | Miromatrix Medical Inc. | Decellularization and recellularization of organs and tissues |
| US20100093066A1 (en) | 2005-08-26 | 2010-04-15 | Regents Of The University Of Minnesota | Decellularization and recellularization apparatuses and systems containing the same |
| JP4599315B2 (ja) | 2006-02-23 | 2010-12-15 | クラレメディカル株式会社 | バイオ人工臓器 |
| CN101066477B (zh) | 2007-05-17 | 2010-10-06 | 中国人民解放军第三军医大学 | 能在体捕获内皮祖细胞的生物人工血管 |
| US8124071B2 (en) | 2007-11-30 | 2012-02-28 | New York Medical College | Methods of reducing transplant rejection and cardiac allograft vasculopathy by implanting autologous stem cells |
| CA2757457C (en) | 2009-03-31 | 2018-05-29 | Regents Of The University Of Minnesota | Decellularization and recellularization of organs and tissues |
| US20120064537A1 (en) | 2010-06-30 | 2012-03-15 | Miromatrix Medical Inc. | Use of perfusion decellularized organs for matched recellularization |
| EP2611472B1 (en) | 2010-09-01 | 2016-02-10 | Regents of the University of Minnesota | Methods of recellularizing a tissue or organ for improved transplantability |
| US9290738B2 (en) | 2012-06-13 | 2016-03-22 | Miromatrix Medical Inc. | Methods of decellularizing bone |
| US20160030637A1 (en) | 2013-03-15 | 2016-02-04 | Miromatrix Medical Inc. | Use of microparticles and endothelial cells with decellularized organs and tissues |
| KR20230061578A (ko) | 2013-03-15 | 2023-05-08 | 미로매트릭스 메디칼 인크. | 섬 세포 재세포화를 위한 관류 탈세포화된 간의 용도 |
| JP6340772B2 (ja) | 2013-11-08 | 2018-06-13 | 東ソー株式会社 | パラメータ変更履歴を管理可能な分析装置 |
| EP3274007B1 (en) | 2015-03-26 | 2020-09-30 | Miromatrix Medical Inc. | Gas filled decellularized extracellular matrix |
| WO2018048899A1 (en) | 2016-09-06 | 2018-03-15 | Micromatrix Medical Inc. | Use of resected liver serum for whole liver engineering |
-
2006
- 2006-08-28 KR KR1020137028378A patent/KR101420585B1/ko active Active
- 2006-08-28 MX MX2008002589A patent/MX2008002589A/es active IP Right Grant
- 2006-08-28 MX MX2014006778A patent/MX350338B/es unknown
- 2006-08-28 PL PL11181797T patent/PL2431063T3/pl unknown
- 2006-08-28 CA CA2618731A patent/CA2618731C/en active Active
- 2006-08-28 CN CN2006800309254A patent/CN101272815B/zh active Active
- 2006-08-28 EP EP11181797.9A patent/EP2431063B1/en active Active
- 2006-08-28 SG SG201101898-3A patent/SG170731A1/en unknown
- 2006-08-28 US US12/064,613 patent/US8470520B2/en active Active
- 2006-08-28 PL PL06790024T patent/PL1928519T3/pl unknown
- 2006-08-28 KR KR1020147007674A patent/KR20140040293A/ko not_active Ceased
- 2006-08-28 EP EP15170077.0A patent/EP2965769B1/en active Active
- 2006-08-28 AT AT06790024T patent/ATE552007T1/de active
- 2006-08-28 KR KR1020087007151A patent/KR101376013B1/ko active Active
- 2006-08-28 JP JP2008528231A patent/JP2009505752A/ja active Pending
- 2006-08-28 ES ES06790024T patent/ES2384721T3/es active Active
- 2006-08-28 MX MX2017011107A patent/MX369594B/es unknown
- 2006-08-28 RU RU2008111503/14A patent/RU2463081C2/ru active
- 2006-08-28 EP EP06790024A patent/EP1928519B1/en active Active
- 2006-08-28 ES ES11181797.9T patent/ES2547139T3/es active Active
- 2006-08-28 CN CN201210287455.7A patent/CN102861359B/zh active Active
- 2006-08-28 AU AU2006282783A patent/AU2006282783B2/en active Active
- 2006-08-28 WO PCT/US2006/033415 patent/WO2007025233A1/en not_active Ceased
-
2008
- 2008-02-11 IL IL189418A patent/IL189418A/en active IP Right Grant
-
2012
- 2012-05-28 RU RU2012122055A patent/RU2635478C9/ru active
- 2012-11-12 JP JP2012248398A patent/JP2013056183A/ja not_active Withdrawn
- 2012-12-21 US US13/725,030 patent/US10441609B2/en active Active
-
2013
- 2013-06-10 US US13/913,974 patent/US10220056B2/en active Active
-
2014
- 2014-07-27 IL IL233821A patent/IL233821A/en active IP Right Grant
-
2015
- 2015-05-07 JP JP2015094591A patent/JP6089062B2/ja active Active
-
2016
- 2016-10-13 JP JP2016201559A patent/JP2017038948A/ja not_active Withdrawn
-
2019
- 2019-01-11 US US16/245,435 patent/US20190343877A1/en not_active Abandoned
- 2019-02-25 JP JP2019031120A patent/JP2019088910A/ja not_active Withdrawn
- 2019-08-29 US US16/554,792 patent/US20200222456A1/en active Pending
-
2021
- 2021-05-14 JP JP2021082285A patent/JP7286708B2/ja active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030087428A1 (en) | 1999-06-07 | 2003-05-08 | Lloyd Wolfinbarger | Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced |
| US6416995B1 (en) | 1999-11-22 | 2002-07-09 | Bio Science Consultants, L.L.C. | Bioreactor mediated recellularization of natural and tissue engineered vascular grafts |
| US6479064B1 (en) | 1999-12-29 | 2002-11-12 | Children's Medical Center Corporation | Culturing different cell populations on a decellularized natural biostructure for organ reconstruction |
| WO2007025233A1 (en) | 2005-08-26 | 2007-03-01 | Regents Of The University Of Minnesota | Decellularization and recellularization of organs and tissues |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101376013B1 (ko) | 기관 및 조직의 세포제거 및 재세포화 | |
| JP2022107023A (ja) | 臓器および組織を脱細胞化および再細胞化する方法 | |
| AU2013224686B2 (en) | Decellularization and recellularization of organs and tissues | |
| AU2017272168B2 (en) | Decellularization and recellularization of organs and tissues | |
| HK1220646B (en) | Decellularization and recellularization of organs and tissues | |
| HK1168565B (en) | Decellularization and recellularization of organs and tissues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20080325 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20110826 Comment text: Request for Examination of Application |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20121124 Patent event code: PE09021S01D |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20130627 Patent event code: PE09021S01D |
|
| A107 | Divisional application of patent | ||
| PA0104 | Divisional application for international application |
Comment text: Divisional Application for International Patent Patent event code: PA01041R01D Patent event date: 20131028 |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20131226 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20140312 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20140312 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| FPAY | Annual fee payment |
Payment date: 20170307 Year of fee payment: 4 |
|
| PR1001 | Payment of annual fee |
Payment date: 20170307 Start annual number: 4 End annual number: 4 |
|
| FPAY | Annual fee payment |
Payment date: 20190228 Year of fee payment: 6 |
|
| PR1001 | Payment of annual fee |
Payment date: 20190228 Start annual number: 6 End annual number: 6 |
|
| FPAY | Annual fee payment |
Payment date: 20200227 Year of fee payment: 7 |
|
| PR1001 | Payment of annual fee |
Payment date: 20200227 Start annual number: 7 End annual number: 7 |
|
| PR1001 | Payment of annual fee |
Payment date: 20210226 Start annual number: 8 End annual number: 8 |
|
| PR1001 | Payment of annual fee |
Payment date: 20240228 Start annual number: 11 End annual number: 11 |
|
| PR1001 | Payment of annual fee |
Payment date: 20250305 Start annual number: 12 End annual number: 12 |