KR101120130B1 - 피이지화 단백질의 응집물 수준을 감소시키기 위한 공정 - Google Patents

피이지화 단백질의 응집물 수준을 감소시키기 위한 공정 Download PDF

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Publication number
KR101120130B1
KR101120130B1 KR1020057004785A KR20057004785A KR101120130B1 KR 101120130 B1 KR101120130 B1 KR 101120130B1 KR 1020057004785 A KR1020057004785 A KR 1020057004785A KR 20057004785 A KR20057004785 A KR 20057004785A KR 101120130 B1 KR101120130 B1 KR 101120130B1
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KR
South Korea
Prior art keywords
peg
pegylated
protein
growth hormone
isomers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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KR1020057004785A
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English (en)
Korean (ko)
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KR20050046011A (ko
Inventor
데니스 엠 보일
존 제이 버클리
개리 브이 존슨
데이비드 이 스테인미어
마이클 토올
세르다 에이켄트
아누래그 에스 래도르
Original Assignee
파마시아 코포레이션
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Priority claimed from US10/646,798 external-priority patent/US20040048315A1/en
Application filed by 파마시아 코포레이션 filed Critical 파마시아 코포레이션
Publication of KR20050046011A publication Critical patent/KR20050046011A/ko
Application granted granted Critical
Publication of KR101120130B1 publication Critical patent/KR101120130B1/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
KR1020057004785A 2002-09-20 2003-09-22 피이지화 단백질의 응집물 수준을 감소시키기 위한 공정 Expired - Fee Related KR101120130B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US41222702P 2002-09-20 2002-09-20
US60/412,227 2002-09-20
US10/646,798 2003-08-25
US10/646,798 US20040048315A1 (en) 2002-08-28 2003-08-25 Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof
US66288403A 2003-09-16 2003-09-16
US10/662,884 2003-09-16
PCT/US2003/029546 WO2004026251A2 (en) 2002-09-20 2003-09-22 Process for decreasing aggregate levels of pegylated protein

Publications (2)

Publication Number Publication Date
KR20050046011A KR20050046011A (ko) 2005-05-17
KR101120130B1 true KR101120130B1 (ko) 2012-04-18

Family

ID=32034217

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020057004785A Expired - Fee Related KR101120130B1 (ko) 2002-09-20 2003-09-22 피이지화 단백질의 응집물 수준을 감소시키기 위한 공정

Country Status (17)

Country Link
EP (1) EP1545428B1 (enExample)
JP (1) JP4633463B2 (enExample)
KR (1) KR101120130B1 (enExample)
AT (1) ATE453662T1 (enExample)
AU (1) AU2003272585B2 (enExample)
BR (1) BRPI0314120B8 (enExample)
CA (1) CA2498886C (enExample)
DE (1) DE60330787D1 (enExample)
DK (1) DK1545428T3 (enExample)
ES (1) ES2337041T3 (enExample)
HK (1) HK1079798B (enExample)
IL (1) IL167538A (enExample)
MX (1) MXPA05003121A (enExample)
PL (1) PL212726B1 (enExample)
PT (1) PT1545428E (enExample)
SI (1) SI1545428T1 (enExample)
WO (1) WO2004026251A2 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA06007848A (es) * 2004-02-09 2006-09-04 Pharmacia Corp Conjugados de antagonistas del receptor de hormona del crecimiento humana modificados quimicamente.
WO2006073846A2 (en) * 2004-12-22 2006-07-13 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
WO2011018515A1 (en) * 2009-08-14 2011-02-17 Novo Nordisk Health Care Ag Method of purifying pegylated proteins
EP2707383B1 (en) * 2011-05-13 2018-04-18 Biogen MA Inc. Methods of preventing and removing trisulfide bonds
WO2013026803A1 (en) * 2011-08-25 2013-02-28 F. Hoffmann-La Roche Ag Cation and anion exchange chromatography method
WO2019129877A1 (en) * 2017-12-29 2019-07-04 F. Hoffmann-La Roche Ag Process for providing pegylated protein composition
CN112107880B (zh) * 2020-09-16 2022-08-09 珠海中科先进技术研究院有限公司 一种低共熔混合物及利用其萃取鲟鱼鱼籽蛋白质的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153265A (en) 1988-01-20 1992-10-06 Cetus Corporation Conjugation of polymer to colony stimulating factor-1

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3641115A1 (de) * 1986-12-02 1988-06-16 Lentia Gmbh Verfahren zur herstellung eines intravenoes anwendbaren und in fluessiger form stabilen immunglobulins
US4847325A (en) * 1988-01-20 1989-07-11 Cetus Corporation Conjugation of polymer to colony stimulating factor-1
US5969109A (en) * 1990-02-28 1999-10-19 Bona; Constantin Chimeric antibodies comprising antigen binding sites and B and T cell epitopes
DK44593D0 (da) * 1993-04-20 1993-04-20 Novo Nordisk As Fremgangsmaade til fremstilling af et polypeptid
ZA955789B (en) * 1994-07-15 1996-03-11 Novo Nordisk As A method of converting a hydrophobic derivative of a polypeptide into the native form
WO1996040731A1 (en) * 1995-06-07 1996-12-19 Mount Sinai School Of Medicine Of The City University Of New York Pegylated modified proteins
DE69635026T2 (de) * 1995-09-21 2006-05-24 Genentech Inc., San Francisco Varianten des menschlichen wachstumshormons
SE9802454D0 (sv) * 1998-07-08 1998-07-08 Pharmacia & Upjohn Ab Production of peptides
SE9904502D0 (sv) * 1999-12-09 1999-12-09 Pharmacia & Upjohn Ab Production of peptides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153265A (en) 1988-01-20 1992-10-06 Cetus Corporation Conjugation of polymer to colony stimulating factor-1

Also Published As

Publication number Publication date
HK1079798A1 (zh) 2006-04-13
EP1545428A4 (en) 2006-03-01
JP4633463B2 (ja) 2011-02-16
PL375443A1 (en) 2005-11-28
SI1545428T1 (sl) 2010-03-31
HK1079798B (zh) 2010-11-19
DE60330787D1 (de) 2010-02-11
JP2006516021A (ja) 2006-06-15
WO2004026251A2 (en) 2004-04-01
EP1545428B1 (en) 2009-12-30
ES2337041T3 (es) 2010-04-20
BRPI0314120B8 (pt) 2021-05-25
EP1545428A2 (en) 2005-06-29
BRPI0314120B1 (pt) 2020-12-29
IL167538A (en) 2015-10-29
PL212726B1 (pl) 2012-11-30
MXPA05003121A (es) 2005-06-22
KR20050046011A (ko) 2005-05-17
CA2498886C (en) 2013-06-25
DK1545428T3 (da) 2010-05-03
BR0314120A (pt) 2005-07-12
WO2004026251A3 (en) 2004-09-02
CA2498886A1 (en) 2004-04-01
PT1545428E (pt) 2010-02-25
AU2003272585A1 (en) 2004-04-08
ATE453662T1 (de) 2010-01-15
AU2003272585B2 (en) 2009-12-10

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