JPS6045848B2 - ヒト成長ホルモンの製造方法 - Google Patents
ヒト成長ホルモンの製造方法Info
- Publication number
- JPS6045848B2 JPS6045848B2 JP55105273A JP10527380A JPS6045848B2 JP S6045848 B2 JPS6045848 B2 JP S6045848B2 JP 55105273 A JP55105273 A JP 55105273A JP 10527380 A JP10527380 A JP 10527380A JP S6045848 B2 JPS6045848 B2 JP S6045848B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- growth hormone
- human growth
- human
- animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- 108010000521 Human Growth Hormone Proteins 0.000 title claims description 48
- 239000000854 Human Growth Hormone Substances 0.000 title claims description 48
- 238000004519 manufacturing process Methods 0.000 title claims description 17
- 241001465754 Metazoa Species 0.000 claims description 34
- 241000282412 Homo Species 0.000 claims description 7
- 102000018997 Growth Hormone Human genes 0.000 claims description 6
- 108010051696 Growth Hormone Proteins 0.000 claims description 6
- 210000001124 body fluid Anatomy 0.000 claims description 6
- 239000010839 body fluid Substances 0.000 claims description 6
- 239000000122 growth hormone Substances 0.000 claims description 6
- 239000000411 inducer Substances 0.000 claims description 5
- 230000002062 proliferating effect Effects 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 78
- 238000000034 method Methods 0.000 description 16
- 239000000725 suspension Substances 0.000 description 13
- 206010028980 Neoplasm Diseases 0.000 description 9
- 238000009792 diffusion process Methods 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 241000699800 Cricetinae Species 0.000 description 5
- 238000011580 nude mouse model Methods 0.000 description 5
- 230000035755 proliferation Effects 0.000 description 5
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- 241000700159 Rattus Species 0.000 description 4
- 230000007910 cell fusion Effects 0.000 description 4
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- 230000028993 immune response Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
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- 239000003814 drug Substances 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
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- 241000287828 Gallus gallus Species 0.000 description 2
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 2
- 241000711408 Murine respirovirus Species 0.000 description 2
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- 235000013330 chicken meat Nutrition 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
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- 229940088597 hormone Drugs 0.000 description 2
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- 101100083507 Caenorhabditis elegans acl-2 gene Proteins 0.000 description 1
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- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000005310 acidophil adenoma Diseases 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000009697 arginine Nutrition 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 229960003444 immunosuppressant agent Drugs 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 208000020788 pituitary gland acidophil adenoma Diseases 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55105273A JPS6045848B2 (ja) | 1980-07-31 | 1980-07-31 | ヒト成長ホルモンの製造方法 |
| IT48956/81A IT1142590B (it) | 1980-07-31 | 1981-07-22 | Procedimento per la produzione di ormone umano della crescita |
| KR1019810002669A KR860000895B1 (ko) | 1980-07-31 | 1981-07-23 | 인체 성장호르몬의 제조방법 |
| GB8122891A GB2083824B (en) | 1980-07-31 | 1981-07-24 | Process for the production of human growth hormone |
| CH4866/81A CH650802A5 (fr) | 1980-07-31 | 1981-07-27 | Production d'hormone de croissance humaine. |
| FR8114524A FR2487852A1 (fr) | 1980-07-31 | 1981-07-27 | Production d'hormone de croissance humaine |
| US06/584,017 US4621053A (en) | 1980-07-30 | 1984-02-27 | Process for the production of human peptide hormones |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55105273A JPS6045848B2 (ja) | 1980-07-31 | 1980-07-31 | ヒト成長ホルモンの製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5731622A JPS5731622A (en) | 1982-02-20 |
| JPS6045848B2 true JPS6045848B2 (ja) | 1985-10-12 |
Family
ID=14403051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55105273A Expired JPS6045848B2 (ja) | 1980-07-30 | 1980-07-31 | ヒト成長ホルモンの製造方法 |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS6045848B2 (enExample) |
| KR (1) | KR860000895B1 (enExample) |
| CH (1) | CH650802A5 (enExample) |
| FR (1) | FR2487852A1 (enExample) |
| GB (1) | GB2083824B (enExample) |
| IT (1) | IT1142590B (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58138395A (ja) * | 1982-02-12 | 1983-08-17 | Hayashibara Biochem Lab Inc | ヒト免疫応答抑制因子の製造方法 |
| FR2523155B1 (fr) * | 1982-03-11 | 1987-10-16 | Hayashibara Biochem Lab | Preparation de facteur de croissance des cellules t humaines |
| BG49718A3 (bg) * | 1983-07-15 | 1992-01-15 | Bio- Technology General Corp | Метод за получаване на полипептид със супероксиддисмутазна активност |
| FR2565995B1 (fr) * | 1984-03-07 | 1987-10-23 | Centre Nat Rech Scient | Cellules hybrides productrices d'un polypeptide determine et obtenu a partir de cellules primaires naturellement capables d'exprimer ce polypeptide ou un polypeptide equivalent, procede pour l'obtention de ces cellules hybrides et application de celles-ci a la production dudit polypeptide |
| FR2581393B2 (fr) * | 1985-05-02 | 1989-07-21 | Grp Genie Genetique | Cellules hybrides productrices d'un antigene caracteristique du virus de l'hepatite b obtenu a partir d'hepatocytes et de cellules etablies de singe, procede pour l'obtention de ces cellules hybrides et application de celles-ci a la production de l'antigene susdit |
| FR2596414B1 (fr) * | 1986-03-28 | 1989-10-06 | Pasteur Institut | Hybridomes obtenus a partir de lymphocytes d'animaux transgeniques portant un gene exprimant une proteine donnee et procede de preparation d'une telle proteine a partir de tels hybridomes |
| USD516320S1 (en) | 2004-07-15 | 2006-03-07 | The Procter & Gamble Company | Surface pattern of a paper product |
| USD510665S1 (en) | 2004-09-29 | 2005-10-18 | The Procter & Gamble Company | Surface pattern for an embossed paper product |
| JP4855803B2 (ja) * | 2006-02-28 | 2012-01-18 | 株式会社フクダ産業 | 呼吸機能検査装置 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4124448A (en) * | 1976-04-09 | 1978-11-07 | The Regents Of The University Of Minnesota | Process for the large scale production of human growth hormone by serial secondary suspension culture |
| GB2016015B (en) * | 1978-01-22 | 1982-05-06 | Hayashibara Co | Method of preparing interferon and preparations containing interferon |
-
1980
- 1980-07-31 JP JP55105273A patent/JPS6045848B2/ja not_active Expired
-
1981
- 1981-07-22 IT IT48956/81A patent/IT1142590B/it active
- 1981-07-23 KR KR1019810002669A patent/KR860000895B1/ko not_active Expired
- 1981-07-24 GB GB8122891A patent/GB2083824B/en not_active Expired
- 1981-07-27 CH CH4866/81A patent/CH650802A5/fr not_active IP Right Cessation
- 1981-07-27 FR FR8114524A patent/FR2487852A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| CH650802A5 (fr) | 1985-08-15 |
| KR830005868A (ko) | 1983-09-14 |
| JPS5731622A (en) | 1982-02-20 |
| GB2083824A (en) | 1982-03-31 |
| GB2083824B (en) | 1984-01-11 |
| FR2487852A1 (fr) | 1982-02-05 |
| IT8148956A0 (it) | 1981-07-22 |
| FR2487852B1 (enExample) | 1984-12-14 |
| IT1142590B (it) | 1986-10-08 |
| KR860000895B1 (ko) | 1986-07-16 |
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