JPS60262055A - 核酸のハイブリツド形成分析法およびそれに用いる試薬系 - Google Patents

核酸のハイブリツド形成分析法およびそれに用いる試薬系

Info

Publication number
JPS60262055A
JPS60262055A JP60116876A JP11687685A JPS60262055A JP S60262055 A JPS60262055 A JP S60262055A JP 60116876 A JP60116876 A JP 60116876A JP 11687685 A JP11687685 A JP 11687685A JP S60262055 A JPS60262055 A JP S60262055A
Authority
JP
Japan
Prior art keywords
rna
probe
sequence
dna
immobilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP60116876A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0531109B2 (cg-RX-API-DMAC7.html
Inventor
ロバート・ジエイ・キヤリコ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of JPS60262055A publication Critical patent/JPS60262055A/ja
Publication of JPH0531109B2 publication Critical patent/JPH0531109B2/ja
Granted legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP60116876A 1984-06-01 1985-05-31 核酸のハイブリツド形成分析法およびそれに用いる試薬系 Granted JPS60262055A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61613284A 1984-06-01 1984-06-01
US616132 1984-06-01
US707420 1985-03-01

Publications (2)

Publication Number Publication Date
JPS60262055A true JPS60262055A (ja) 1985-12-25
JPH0531109B2 JPH0531109B2 (cg-RX-API-DMAC7.html) 1993-05-11

Family

ID=24468178

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60116876A Granted JPS60262055A (ja) 1984-06-01 1985-05-31 核酸のハイブリツド形成分析法およびそれに用いる試薬系

Country Status (3)

Country Link
US (1) US4833084A (cg-RX-API-DMAC7.html)
JP (1) JPS60262055A (cg-RX-API-DMAC7.html)
ZA (1) ZA853756B (cg-RX-API-DMAC7.html)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2069948A1 (en) * 1989-12-01 1991-06-02 Brian Wylie Pontius Promotion of high specificity molecular assembly
ES2145750T3 (es) 1991-11-14 2000-07-16 Dgi Inc Kit y dosificado que utiliza la hibridacion no radioactiva.
DK145493D0 (da) * 1993-12-23 1993-12-23 Dako As Antistof
CA2139070C (en) * 1994-12-23 2010-03-30 Burton W. Blais Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction
US5753787A (en) * 1995-04-10 1998-05-19 Yale University Nucleic acids encoding ancylostoma secreted protein
US6187551B1 (en) * 1995-09-19 2001-02-13 Cytochem, Inc. Detection and quantitation of 8-OH-Adenine using monoclonal antibodies
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
GB2324370B (en) * 1997-04-14 1999-03-03 Stuart Harbron Detection of hybrid double-stranded DNA with antibody after enzyme degradation of excess single-standed DNA
US6225053B1 (en) 1997-12-12 2001-05-01 Digene Corporation Detection of hepatitis B virus
AU746061B2 (en) 1997-12-12 2002-04-11 Qiagen Gaithersburg, Inc. Assessment of human papilloma virus-related disease
US5994079A (en) 1998-02-06 1999-11-30 Digene Corporation Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US7399589B2 (en) * 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US6686151B1 (en) 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US6183956B1 (en) * 1998-03-31 2001-02-06 Tularik, Incorporated High throughput in vitro screening assays for transcription modulators
WO1999050460A1 (en) * 1998-03-31 1999-10-07 Tularik, Inc. HIGH THROUGHPUT ASSAY FOR DETECTION OF mRNA IN CELLS
US6232068B1 (en) 1999-01-22 2001-05-15 Rosetta Inpharmatics, Inc. Monitoring of gene expression by detecting hybridization to nucleic acid arrays using anti-heteronucleic acid antibodies
KR100341827B1 (ko) * 2000-01-29 2002-06-26 박호군 아미노-rna에 대한 항체 및 이의 제조방법
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
EP1451356A1 (en) * 2001-11-23 2004-09-01 Simon Fredriksson Method and kit for proximity probing with multivalent proximity probes
CA2542542C (en) 2003-10-21 2015-09-15 Orion Genomics Llc Method of detecting a quantity of methylation at a locus
CN102203293A (zh) * 2008-10-27 2011-09-28 奇亚根盖瑟斯堡股份有限公司 快速结果杂交捕获测定和系统
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
US9797000B2 (en) 2009-05-01 2017-10-24 Qiagen Gaithersburg Inc. Non-target amplification method for detection of RNA splice-forms in a sample
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
CN102822189B (zh) * 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
US20130059736A1 (en) 2010-02-05 2013-03-07 Institute For Systems Biology Methods and compositions for profiling rna molecules
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー. 核酸の配列特異的な精製及び多重分析のための方法及び組成物
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4732847A (en) * 1981-06-09 1988-03-22 University Of Hawaii Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
US4743535A (en) * 1984-11-07 1988-05-10 Miles Inc. Hybridization assay employing labeled probe and anti-hybrid

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIOCHEM.BIOPHYS.RES.COMMUN=1969 *
BIOCHEMISTRY=1978 *
BIOCHEMISTRY=1979 *
NUCLEIC ACID RESEACH=1982 *
PROC.NATL.ACAD.SCI.USA=1982 *

Also Published As

Publication number Publication date
ZA853756B (en) 1986-01-29
JPH0531109B2 (cg-RX-API-DMAC7.html) 1993-05-11
US4833084A (en) 1989-05-23

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