JPS5929620A - Preventing agent for carious tooth - Google Patents

Abstract

PURPOSE:A preventing agent for carious teeth containing at least one of an extract of a plant source material, e.g. ginger, cumin or celery seed, with an organic solvent or steam distillation product as an active constitutent. CONSTITUTION:A preventing agent for carious teeth, obtained by incorporating 0.001-5wt%, preferably 0.1-2wt%, at least one of an extract obtained by extracting a plant source material shown in the table with an organic solvent or a steam distillation product with a composition for the oral cavity. A dentifrice, e.g. toothpaste, troche, mouthwash, solid mouth refrigerant, liquid mouth refrigerant, chewing gum or embrocation, etc. may be cited as the composition for the oral cavity suitable for the incorporation. A dentine reinforcing agent, e.g. sodium monofluorophosphate, a germicide, e.g. chlorohexidine, an anti-inflammatory agent, e.g. dihydrocholesterol, a tissue activating agent, e.g. hinokitiol, and lysozyme or common salt, etc. may be incorporated with the composition.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention is directed to the treatment of Streptococcus mutans (Streptococcus mutans), which is significantly involved in the formation of dental caries (caries).
The present invention relates to an anti-caries agent which exhibits an excellent inhibiting effect on the growth of oral microorganisms such as 0 coccus mutans, is capable of preventing dental plaque formation involving the oral microorganisms, and is useful for preventing dental caries. The anti-caries agent is used in toothpastes,
Lozenges, mouthwashes, chewing gums,
It is a natural plant-based anti-caries agent that can be used by incorporating it into oral compositions such as mouth fresheners.

More specifically, the present invention includes ginger, cumin, celery seed, tarabon, lovage, valerian, origanum, hop, ampulette seed, dill seed, benny royal, puthuly 7, tansy, wineries, ephedra, mountain) An anti-caries agent characterized by containing as an active ingredient at least one type of organic solvent extract or steam distillate of a plant source material selected from the group consisting of Hosakashi, Bakumonfu, Hireshiko, and Gokapi. Regarding.

It is said that dental caries is caused by oral microorganisms, dental plaque, ingredients in food, especially sugar, and tooth structure. Children are more likely to develop dental caries from a young age, and it is said that 3-year-old children already have caries in their baby teeth as high as 902%.

Dental plaque is formed on the tooth surface by oral bacteria, food residue, salivary organic matter, etc., but Streptofuccus mutans, one of the oral bacteria, synthesizes dextran-like polysaccharides from sucrose and stains the teeth. It is known that it promotes the formation of plaque, and that it also produces acids from sucrose and other sugars, boils them into the plaque, and causes tooth demineralization, leading to dental caries. There is.

Therefore, suppressing the growth of oral bacteria such as Streptococcus mutans and blocking the formation of dental plaque are effective means for preventing the occurrence of dental caries.

Methods for preventing the formation of such dental plaque include adding a bactericide such as chlorhexidine to oral preparations to suppress the growth of oral bacteria, or adding polysaccharide-degrading enzymes such as dextranase to oral preparations. There are known methods for preventing the formation of dental plaque.

3- Furthermore, several proposals are known regarding oral compositions that utilize organic solvent extracts of natural plant source materials. For example, in Japanese Patent Application Laid-open No. 57-564.15, prevention of bad breath,
For the prevention of periodontal disease, Aristolochia, Aristolochia, Aristolochia, Ganoyutsu, Fuubushi, Fukanfu, and Seimonko are used as ingredients that exhibit antibacterial activity against the oral bacteria 7 Zobacterium nucleatum, which is said to cause bad breath and periodontal disease. Oral compositions have been proposed containing organic solvent extracts of plants selected from A. chinensis and Sanna.

Also, JP-A-57-58610, JP-A-57-5861
No. 1 and Japanese Patent Application Laid-open No. 57-58612 contain the following ingredients: ryokyo, ginseng, kashu, and kuromoji as ingredients that inhibit the formation of dental plaque by oral microorganisms such as Streptococcus mutans, which are involved in the formation of cavities. ,
Nantenjitsu, hawthorn, button skin, fuju, onji, eyounin, perilla, shiso, arnica, cardamom,
Zenko, Korean ginseng, Youkat, Youbaihi,
4- An oral composition containing an organic solvent extract of a plant selected from Guiou, Akamegashiwa, and Gennoshoko has been proposed.

Furthermore, Japanese Patent Application Laid-open No. 57-58613 discloses an organic solvent extraction of plants selected from melilot, Kouka, Kinginjiro, and Shicon as an oral composition suitable for the prevention and treatment of periodontal diseases such as gingivitis. Oral compositions containing substances have been proposed.

The present inventors have been conducting research on methods for inhibiting the proliferation of Streptococcus mutans, a typical oral bacterium that is largely involved in the formation of dental plaque that causes dental caries.

As a result, plant-sourced materials that were previously not known to contain substances that inhibit oral microorganisms involved in tooth decay, such as Streptococcus mutans, have been used, especially ginger, cumin, celery seed, tarabon, and lovage. , valerian, origanum, hops, ampulette seed, dill seed, benny royal, puthu leaf, 5-tansy, wineries, ephedra, mountain pile, spicy, barley winter,
A steam distillate or an organic solvent extract of a plant-source material selected from the group consisting of Streptococcus mutans and Streptococcus mutans, which has a significant role in caries formation and plaque formation, can be used to It was discovered that it exhibits an excellent inhibitory effect against microorganisms and has flavor preference at the concentration used.

Furthermore, it has been found that by appropriately selecting or selectively combining the above-mentioned plant source materials, it is possible to provide an excellent anti-caries agent that effectively prevents dental caries and leaves a pleasant scent in the oral cavity.

Accordingly, it is an object of the present invention to provide an excellent anti-caries agent derived from natural plant source materials.

The above objects and further objects and effects of the present invention are as follows:
It will become clearer from the explanation below.

Oral compositions suitable for incorporating organic solvent extracts or steam distillates of plant-source materials according to the invention, such as toothpastes, powdered toothpastes, troches, mouthwashes, solid mouth fresheners, liquid mouth freshener,
Examples include chewing gum and liniments.

The present invention provides ginger in the oral composition as exemplified in -.
, cumin, celery seed, tarabone, lobein, valerian, origanum, hops, ampulette seed, dill seed 1 benny royal, puthuly 7, tansy, wineries, ephedra, mountain pile, spicy chili, barley mondo, joe ko and goka. The present invention relates to an anti-caries agent which effectively prevents the occurrence of dental caries by incorporating at least one steam distillate, organic solvent extract, or a mixture thereof selected from the group consisting of skin into an oral composition.

The organic solvent extract from the above plants in the present invention can be obtained by extracting the plants with an organic solvent. Examples of organic solvents used in such extraction processing include, for example,
Polar solvents such as methanol, ethanol, n-propyl alcohol, isopropyl alcohol, 7-aceane, propylene glycol, glycerin, ethyl acetate, or e.g.
- Non-polar solvents such as hexane, cyclohexane, petroleum ether, ligroin, carbon tetrachloride, methylene chloride, ethylene dichloride, benzene, toluene, or
A mixture of these can be exemplified. Further, the polar solvent may be used in the form of a water-containing solvent, for example, a water-containing solvent having a water content of about 5% to about 90%.

Specific examples of plant source materials used for extraction include roots or rhizomes such as eba, ginger, lovage, valerian, hosara, barley, barley, and cumin, celery, and ambrette. , dill, wild pine, and seeds of cypress, as well as tarabon, origanum, benny royal,
In the case of buchu, tansy, and ephedra, examples include leaves, stems, or whole plants; in the case of hops, female panicles; and in the case of lovage, seeds, leaves, stems, and other whole plants can be exemplified.

In addition to fresh products, semi-dry products and dried products can be used as the source material for grains as described in Section 8-2 above, and dried products are particularly preferred.

Another example is the wineries obtained as the residue left over when wine is distilled to produce brandy.

Furthermore, in the present invention, commercially available extracts and commercially available essential oils obtained from the above-mentioned foreign plant source materials can be utilized in exactly the same manner.

Extraction and drying operations and extraction conditions vary depending on the selection, but for example, about 0.5 to about 50 times the weight of the organic solvent is added to the plant source material, and the mixture is heated at room temperature to the boiling point temperature of the solvent used, for example, about 5 times the organic solvent.
This can be carried out by standing still or stirring for a minute to about 24 hours. After performing the extraction operation in this manner, an extract of the plant can be obtained by removing residual insoluble particles by, for example, centrifugation, microfiltration, squeezing, or other solid separation means.

Furthermore, if necessary, an organic solvent can be further added to the insoluble solid content extraction residue and the same operation can be repeated for extraction.

In the present invention, it is also possible to use a concentrate obtained by removing the solvent from the extract obtained from the plant source material by the above-mentioned method.

Furthermore, the extract obtained by the above method can be decolorized and concentrated using an adsorbent such as activated carbon, activated alumina, silica gel, hecytonite, acid clay, or diatomaceous earth.

To obtain a steam distillate from the above-mentioned plant source material, use the plant or its dried or pulverized material as a raw material, charge the raw material into a distillation kettle or other suitable container, and add steam under normal pressure or reduced pressure. It can be obtained by cooling the aromatic components distilled out together with the blown-in steam and then removing the separated moisture. Alternatively, it can be obtained by extracting the distillate from steam distillation using an organic solvent and removing the organic solvent from the extract by means such as distillation = 1
0- I can do it.

In the present invention, the blending ratio of the anti-caries aromatic composition comprising the organic solvent extract or steam distillate is about 0.001 to about 5% by weight based on the oral composition.
A particularly preferred amount is about 0.1 to about 2% by weight. The organic solvent extract or steam distillate of the plant source material according to the present invention can be blended without any restrictions with other raw materials that are blended into ordinary oral compositions, and may be mixed as appropriate depending on the type of oral composition. You can choose. For example, in the case of toothpaste, abrasives such as dicalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, silicic anhydride, magnesium carbonate, and aluminum hydroxide; sodium lauryl sulfate;
Foaming agents such as sodium lauroyl sarcosinate, sodium coconut 7glyceride sal 7ate, sodium coconut monoglyceride sulfonate and sucrose fatty acid ester; humectants such as glycerin, sorbitol, propylene glycol; gum tragacanth, gum karaya, iritus moss, Binder such as methylcellulose, carboxymethylcellulose; peppermint oil, spearmint oil, menthol, anethole, wintergreen, cinnamon, clove, vanilla, cassia and other spices, and flavorings such as flora flavor, saccharin, glycyrrhizin, stevioside, neohesperinone. Examples include sweeteners such as hydrochalcone; and preservatives. In the present invention, a toothpaste can be obtained by a conventional method by kneading the steam distillate or organic solvent extract of the plant source material with the ingredients as exemplified above with water.

In the case of troches, mouthwashes, chewing gums, mouth fresheners, etc., the compounding materials can be appropriately selected depending on the properties required for each product.

In the present invention, in addition to the steam distillate or organic solvent extract of the plant source material, active ingredients of the oral composition include, for example, Vium monofluorophosphate, stannous heptadide, and F fluoride. Bactericidal agents such as chlorhexinone; Anti-inflammatory agents such as dihydrofesterin, shrimp dihydrocholesterin, allantoin and its derivatives, epsilonami/caproic acid and tranexamic acid; hinokitiol and sodium copper chlorophyllin, etc. A tissue excipient; lysozyme and salt can also be blended.

The anti-caries agent according to the present invention includes ginger, cumin, celery seed, tarabon, lovage, valerian, origanum, hops, ambrette seed, dill seed, benny royal, futury 7, tannu, wineries, ephedra, elm, It consists of a steam distillate or an organic solvent extract of at least one material selected from the plant group consisting of Hosara, Bakumonto Jheiko, and Gokapi, and the steam distillate or extract is administered orally. By incorporating it into the composition,
It prevents tooth decay by inhibiting the proliferation of Streptococcus mutans13- and suppressing the formation of dental plaque.

Hereinafter, several embodiments of the present invention will be explained in more detail with reference to Examples.

Example 150g of dry powder of renshiko and 300g of 60% ethanol
was added, stirred at 75° to 80°C for 1 hour to carry out the extraction process, and the solvent was removed by filtration.
Extraction treatment was carried out in the same manner using 200 g of ethanol. The alcoholic extracts were combined and concentrated under reduced pressure to obtain 45 g of a brown citrus extract concentrate.

Example 1 Peptone 1%, yeast extract 0.5%, sucrose 2%, pH
A 7.0 agar medium was prepared, sterilized and cooled, and Streptococcus mutans (S.
treptcoccus mutans) I I D
Add 2% culture solution of strain 973 (E49) to the above agar medium, and immediately place 121 cells of each strain in a sterile Petri dish with a diameter of 80 mm.
It was dispensed into 4-ml volumes.

Next, a paper disk with a diameter of 8 mm was impregnated with the palm extract obtained in Experimental Example 1 and the commercially available Benny Royal essential oil, and placed on the center of the agar medium of the Petri dish, and heated for 30 minutes.
The cells were cultured at ℃ for 3 days. The agar medium is transparent before culturing, but becomes cloudy due to the proliferation of Streptococcus mutans. Since the area around the paper disk where bacterial growth was inhibited still remained transparent, this clear zone (hereinafter referred to as closed circle diameter) was measured to evaluate antibacterial properties. The results are shown in Table 1.

Table 1 As a result, both moth palm extract and orinum essential oil,
A remarkable antiproliferative effect on Streptococcus mutans was observed.

Example 2 Growth of Streptofuccus mutans was carried out in the same manner as in Example 1 using extracts obtained from pond plant materials, commercially available extracts, and essential oils. The blocking effect was investigated. Table 2 shows those that had a remarkable effect of inhibiting the growth of the bacteria.

Table 2 Example 2 Effect of inhibiting the growth of S. mutans using the paper disc method 2 Essential oils of Tarabon, Origanum and Benny royale, and Coconut and Goka bark in a heart-in-7 Eudyonbu 17-Yon liquid medium supplemented with 2% sucrose 0.5% extract of
Streftococcus, which had been added and pre-cultured to this.
The culture solution of S. mutans lID973' (E49) was reduced to 0.
．． 5% was added and cultured anaerobically at 37°C for 24 hours. Next, the culture solution was treated with a mixer to disperse the precipitate, and then the absorbance (turbidity) at 550 nm was measured to determine the amount of dental plaque formed by Streptococcus mutans. The results are expressed as a percentage when the amount of plaque formation in the control (no sample added) is set as 100, and is shown in Table 3. In addition, the pH of the culture solution was measured, and the presence or absence of acid production was also examined.

18- Table 3 Amount of plaque formation As is clear from the results in Table 3, the samples listed in Table 2 had a remarkable anti-proliferation effect on Streptococcus mutans even in liquid culture.

19-

Claims (1)

[Claims] 1. Nonjar, cumin, celery seed, tarabon, lovage, valerian, origanum, hop, ampulette seed, dill seed, benny royal, puthuly 7, tansy, wineries, ephedra, mountain rut, spicy, barley, winter. An anti-caries agent characterized in that it contains as an active ingredient at least one type of organic solvent extract or steam distillate of a plant source material selected from the group consisting of Saccharomyces cerevisiae and Gokapi.