JPS6258329B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention is an oral composition that suppresses the formation of dental plaque and prevents dental caries and periodontal disease by incorporating an organic solvent extract (crude extract) of a plant selected from Physcomitrella japonica, Rhubarb, Rhubarb, and Prunus elegans. Regarding. Dental plaque that adheres to the tooth surface is made up of about 70% bacteria, about 20% polysaccharide formed by bacteria, and about 10% food residue, and is firmly stuck to the tooth surface. The acid stored inside demineralizes the enamel, and the bacteria and toxins produced are said to cause gingivitis, periodontitis, and even pyorrhea, two major oral diseases. It is attracting attention as a cause of caries and alveolar pyorrhea. This dental plaque is formed by oral bacteria, mainly Streptococcus miutans, using sucrose present in the oral cavity. That is, Streptococcus miutans produces GTF (glucosyltransferase, dextran synthase), which synthesizes sticky polysaccharides such as dextran and mutan from sucrose. This synthesized polysaccharide then attracts Streptococcus miutans and other bacteria (pathogens), forming dental plaque with a certain bacterial flora. Furthermore, bacteria such as Streptococcus miutans produce acids using various sugars, and this acid demineralizes the enamel surface by staying in the walls of polysaccharides and bacteria. Furthermore, dead bacteria and various substances produced,
Toxins have a negative effect on the gums and act as a cause of pyorrhea. Therefore, suppressing and preventing the formation of dental plaque is
This is extremely effective in preventing caries and periodontal disease. Taking these points into consideration, the present inventors have investigated caries, caries,
As a result of intensive research into oral compositions suitable for the prevention of periodontal diseases and screening of various plants as natural additives, we found that organic solvent extracts of Streptococcus miutans, Rhubarb, Rhubarb, and Gennoshyoko were derived from Streptococcus miutans. of
It has been found that the organic solvent extract suppresses the formation of dental plaque due to GTF, and is therefore suitable for preventing caries and periodontal disease as an active ingredient of an oral composition,
The present invention has been accomplished. The present invention will be explained in detail below. The oral composition according to the present invention includes toothpaste, toothpaste,
It is used as toothpaste such as water toothpaste, mouthwash, troche, pasta, liniments, chewing gum, gargling tablets, etc., and the active ingredient is one of the organic solvent extracts of Oriental japonica, Rheum rhubarb, Red rhubarb, Rhubarbium japonica, Gennosyoko, etc. It is made by blending two or more types. As the organic solvent used for extracting the plants, lower alcohols such as methanol and ethanol, polar solvents such as acetone, ethyl ether, and ethyl acetate, and nonpolar solvents such as benzene, hexane, and petroleum ether can be used. Mixed solvents such as aqueous ethanol may also be used. The organic solvent extract used as an active ingredient in the present invention is obtained by extracting the above-mentioned plants with one type or a mixture of two or more of these organic solvents, or a mixture of these organic solvents and water, and removing the extraction residue. can be produced by distilling off the solvent from the extract, if necessary. Note that a known method can be used as the extraction method, for example, a method such as digesting the dried powder of the plant in an organic solvent can be used. The amount of the organic solvent extract incorporated into the oral composition is 0.001 to 5% (wt%, same hereinafter) of the total,
In particular, it is preferably 0.005 to 2%. As other components of the present invention, appropriate components are used depending on the type of oral composition. For example, in the case of toothpaste, dibasic calcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, amorphous silica, crystalline silica, aluminosilicate, aluminum oxide, aluminum hydroxide, abrasives such as resin, carboxylic Methylcellulose, hydroxyethylcellulose, alginate, carrageenan, gum arabic,
Binder such as polyvinyl alcohol, thickening agent such as polyethylene glycol, sorbitol, glycerin, propylene glycol, sodium lauryl sulfate, sodium dodecylbenzenesulfonate,
Hydrogenated Coconut Fatty Acid Monoglyceride Sodium Monosulfate, Sodium Lauryl Sulfoacetate,
Foaming agents such as sodium N-lauroyl sarcosinate, N-acylglutamate, and sucrose fatty acid esters, essential oils such as peppermint and spearmint, fragrance materials such as l-menthol, carvone, eugenol, and anethole, and saccharin. It is produced by mixing ingredients such as sodium, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perillartin, sweeteners such as p-methoxycinnamic aldehyde, preservatives, etc. with water and according to a conventional method. Furthermore, in mouthwashes and other mouthwashes, ingredients are appropriately blended depending on the properties of the product. In the present invention, in addition to the above-mentioned organic solvent extract of plants, lysozyme chloride, dextranase, lytic enzyme, mutanase, chlorhexidine, sorbic acid, alexidine, hinokitiol, cetylpyridinium chloride, alkylglycine, alkyldiaminoethylglycine are used. Salt, allantoin, ε-aminocaproic acid, tranexamic acid, azulene, vitamin E, sodium monofluorophosphate, sodium fluoride, fluoride 1
Active ingredients such as tin, water-soluble primary or secondary phosphates, quaternary ammonium compounds, sodium chloride, etc. can also be incorporated. The oral composition according to the present invention contains one or more organic solvent extracts of plants selected from Streptococcus miutans, Rhubarb, Rhubarb, and Gennoshiwa. This effectively inhibits the formation of caries and periodontal disease. Next, experimental examples will be shown to specifically explain the effects of the present invention. [Experimental example] 300 ml of methanol was added to 100 g of dry rhubarb powder, and the mixture was refluxed for 1 hour on a water bath at 70 to 80°C with a cooling tube attached. Next, the methanol fraction was separated, fresh methanol was added to the residue, and the same reflux was repeated twice, for a total of three extraction operations. The methanol extract was concentrated under reduced pressure to form a brown paste.
37.0g was obtained. The other plants shown in Table 1 were operated in the same manner as above to obtain methanol extracts of each plant. Next, the above extract was used as a test sample, and its degree of inhibition of plaque formation was evaluated by the reaction adhesion method described below. Reactive deposition method 1% sucrose, 0.01% fungicide (NaN ₃ ) and heat sterilized Streptococcus miutans 6715
Add 0.1% of the above test sample to 0.05M phosphate buffer (PH7.0) containing the bacterial cells of the strain, and add the extracellular enzyme containing the GTF of Streptococcus miutans strain 6715 prepared in advance. plus 37
℃ for 16 hours. After the reaction, wash twice with water, then add the same amount of water as the reaction system, perform ultrasonic treatment for about 20 seconds to uniformly suspend the plaque, and then use a photometer to disperse the plaque at 550 nm. The absorbance (turbidity) was measured to determine the amount of attached plaque. The results are shown in Table 1. The results are expressed as a percentage, with the amount of plaque attached to the control to which no test sample was added as 100%.

[Table] From the above results, it was found that the methanol extracts of each plant shown in Table 1 effectively suppress the formation of dental plaque. Examples are shown below. Note that all % indicates weight %. Furthermore, in the following examples, methanol crude extracts of plants were used. [Example 1] Toothpaste dicalcium phosphate dihydrate 50.0% Glycerin 20.0 Carboxymethyl cellulose 1.0 Sodium lauryl sulfate 1.0 Fragrance 1.0 Herbs 0.1 Rhubarb 0.1 Satucalin 0.1 Sodium fluoride 0.1 Water Remaining 100.0% [Example 2] Toothpaste dicalcium phosphate dihydrate 50.0% Sorbit 10.0 Glycerin 10.0 Carboxymethyl cellulose 1.0 Sodium lauryl sulfate 2.0% Fragrance 1.0 Satucalin 0.1 Red-eye wrinkle 0.1 Sodium monofluorophosphate 0.3 Ethanol 2.0 Mutanase 0.1 Water Remaining 100.0% [Example 3] Toothpaste Calcium carbonate 50.0% Glycerin 20.0 Carrageenan 0.5 Carboxymethyl cellulose 1.0 Lauryl diethanolamide 1.0 Sucrose monolaurate 2.0 Fragrance 1.0 Red-breasted wrinkles 0.1 Gennosyoko 0.05 Satucalin 0.1 Chlorhexidine 0.005% Dextranase 0. 01 Water remaining 100.0% [Example 4] Toothpaste dicalcium phosphate dihydrate 50.0% Glycerin 20.0 Carboxymethyl cellulose 2.0 Sodium lauryl sulfate 2.0 Fragrance 1.0 Satukarin 0.1 Iobaihi 0.1 Gennosyouko 0.05 Chlorhexidine 0.01 Water Remaining 100.0% [Example 5] No toothpaste Hydrosilicic acid 30.0% Glycerin 30.0 Sorbit 20.0 Carboxymethyl cellulose 1.0% Sodium lauryl sulfate 2.0 Fragrance 1.0 Satucalin 0.1 Rhubarb 0.1 Rhubarb 0.1 Ethanol 2.0 Water Remaining 100.0% [Example 6] Toothpaste dicalcium phosphate dihydrate 50.0% Calcium carbonate 30.0 Glycerin 10.0 α-olefin sulfonate 1.0 Fragrance 1.0 Saccharin 0.1 Rhubarb 0.2 Sodium monofluorophosphate 0.1 Dextran 0.5 Water Remaining 100.0% [Example 7] Liquid toothpaste Sodium polyacrylate 50.0% Glycerin 30.0 Fragrance 0.9 Saccharin 0.1 Chlorine Kisidine 0.01 Iobaihi 0.05 Gennosyoko 0.05 Ethanol 3.0 Linoleic acid 0.05 Water Remaining 100.0% [Example 8] Mouthwash ethanol 20.0% Fragrance 1.0 Satucalin 0.05 Sodium monofluorophosphate 0.1 Chlorhexidine 0.01 Lauryl diethanolamide 0.3 Mouthwash 0.1 % Red mega wrinkle 0.05 water remaining 100.0% [ Example 9] Gargle tablet for sodium hydrogen carbonate 54.0 % 2.0 polyethylene glycol 3.0 oleic acid sodium (absolute) 13.6 fragrance 2.0 oleic acid 0.0 oleic acid 0.1 sodium oleic acid 0.1 chlorhexidine 0.05 Mega Shiwa 0.1 Gennoshiyoko 0.05 100.0 % [Example 10] Gum pine surge cream white petrolatum 8.0% Propylene glycol 4.0 Stearyl alcohol 8.0% Polyethylene glycol 4000 25 〃 400 37 Sucrose stearate 0.5 Rhubarb 0.1 Rhubarb 0.2 Dextranase 1.0 Water Remaining 100.0% [ Example 11 Chuyu Ingham Gum Base 43.8% Calcium Carbonate 2.0 Water Candy 15.0 Powdered Sugar 30.0 Sucrose Palmitate 1.0 Fructose 4.0 Maltose 3.0 Rhubarb 0.1 Red Root 0.1 Flavor 1.0 100.0%

Claims (1)

[Claims] 1. An oral composition characterized by containing one or more organic solvent extracts of plants selected from Physcomitrella japonica, Rhubarb, Rhubarb, and Prunus elegans.