KR102547873B1 - Oral composition - Google Patents

Abstract

The present invention relates to a composition for oral cavity and a method for preparing the same, and according to the present invention, the anti-inflammatory effect in the oral cavity is maximized, or the effect of improving periodontal disease is maximized.

Description

Oral composition {Oral composition}

It relates to a composition for oral cavity including a toothpaste rinsing liquid and the like, and a method for preparing the same.

Tooth tissue is composed of enamel, cementum and dentin. The outside of the tooth exposed to the outside is covered with enamel, the outside of the tooth root portion located in the alveolar bone is covered with cementum, and dentin exists inside them. In dentin, fine tubes called dentinal tubules are distributed throughout the dentin.

Tooth ache symptoms are sensitive when the exposed dentin part of the tooth comes into contact with cold air or irritating food. Toothache symptoms can range from mild symptoms to intense and persistent pain. In addition, since teeth do not regenerate by nature, taking painkillers or anti-inflammatory drugs is not a fundamental solution to the ache, so various treatments have been developed to alleviate the symptoms of ache.

There are two main treatments for syringitis: dentinal tubule sealing and pulpal nerve desensitization. Dentinal tubule sealing method is a method to restore enamel lost by filling exposed dentinal tubules with resin or applying fluoride, etc., and pulp nerve desensitization method is that neurotransmission is controlled by an appropriate ratio of sodium ions and potassium ions. It is a treatment that prevents the nerves from feeling the pain by artificially increasing the content of potassium ions in consideration of However, these professional treatment procedures are cumbersome to go to the dentist, so toothpastes for cold teeth that can treat or prevent cold teeth in everyday life are sold.

These dentifrices contain active ingredients useful for the above two treatment methods. However, among these, toothpaste containing dibasic potassium phosphate component to exhibit pulpal nerve desensitization effect takes as long as 15 days, so toothpaste with efficacy by dentinal tubule sealing method, which shows efficacy relatively quickly, is preferred. there is.

However, in the case of a toothpaste specializing in toothpaste that utilizes the conventional dentinal tubule sealing effect, it contains fine powder of active ingredients to seal the dentinal tubules. There was a problem that the customs could not be sealed and exposed again.

On the other hand, the causes of halitosis are largely classified into oral, extraoral, and psychogenic causes. Among them, oral causes account for about 80 to 90% of the causes of bad breath. decrease in the amount and activity of oral microbes and oral diseases such as tongue plaque attached to the dorsal surface of the tongue and untreated dental caries and periodontal disease.

On the other hand, among oral diseases, dental caries and periodontal disease cause various clinical symptoms such as pain, masticatory dysfunction, destruction of periodontal tissue and bad breath, and are known to be the main factors that cause tooth loss, and changes in dietary life are the cause of dental caries. elements are on the rise.

It is a method of inhibiting the growth of oral pathogens that live in dental bacterial film and cause dental caries, periodontal disease, and bad breath. It has been developed as an inhibitor and treatment agent for apical infection.

However, since antibiotics can cause the appearance of resistant bacteria in the oral cavity and mycosis, along with systemic side effects on our body, it is difficult to use them for a long time, and there is a disadvantage in that they can be used only as a therapeutic agent. In addition, in the case of fluoride, which is an antibacterial agent, the effect of preventing caries is clear, but no effect on periodontal, dental pulp, or apical diseases has been reported yet. In addition, antibacterial agents used in toothpaste, mouthwash, or other mouthwashes include sanguinarine, listerine, peroxide, or chlorohexidine. However, Sanguinarine has a disadvantage that its effect on bacteria in the oral cavity is unclear and its effect on periodontal disease treatment is not only unclear, but also its price is high. Although it has an action, it has the disadvantage that harmful effects may appear on tissues when used for a long time. Peroxide, which has recently been added for whitening effect, has a toxic effect on bacteria, but at the same time shows a toxic effect on human tissues, so there is only a safety problem. In addition, bacteria resistant to peroxides sometimes appear in bacteria. In addition, chlorhexidine is known to be the best among the agents known to date as a preventive and therapeutic agent for periodontal disease as well as to inhibit plaque formation, but causes irritation to tissues, discoloration and denaturation of tissues, and particularly strong irritating taste and strong odor. In addition to this, there are also disadvantages that cannot be used for a long period of time for treatment or prevention, such as causing mycosis and carcinogenicity, limiting its use in pregnant women (Contemporary periodontics [ed. R.J. Genco et al.], p161-169, p368-369, p433, C.V. Mosby Company, St. Louis, 1990).

Accordingly, efforts are being made to develop natural materials excellent in the prevention, improvement, or treatment of disorders or diseases occurring in the oral cavity, and to improve the efficacy of the developed natural materials, without any concerns about the above-mentioned burial use. there is.

The problem to be solved by the present invention is to provide an oral composition and a method for preparing the same, which are excellent in improving the effects of disorders, diseases or various harmful symptoms occurring in the oral cavity, and in particular, have a maximized anti-inflammatory effect.

In order to solve the above problems,

The present invention provides a composition for oral cavity containing a natural material having excellent anti-inflammatory effect in the oral cavity as an active ingredient and a method for preparing the same.

More specifically, the present invention provides an anti-inflammatory composition for oral use containing Centella asiatica extract, corn unsaponifiable extract and silver leaf extract as active ingredients and a method for preparing the same.

The present inventors have confirmed that Centella asiatica, corn unsaponifiable extract, and rhubarb extract have anti-inflammatory and antibacterial effects while undergoing a screening process for a number of natural materials that can be used in the human body. However, inflammatory diseases in the oral cavity, including periodontal disease, are conditions that are caused and aggravated by complex causes, and the use of Centella asiatica, corn unsaponifiable extract, or silver leaf melon alone has antibacterial action against the causative bacteria of inflammation, inflammation-inducing factors It was confirmed that it is difficult to exhibit complex and wide-ranging effects ranging from suppression of activation, and suppression and regeneration of connective tissue disintegration and alveolar bone disintegration, and therefore does not exhibit an effective effect as an anti-inflammatory composition for oral use. However, it was confirmed that when Centella asiatica extract, corn unsaponifiable extract, and silver leaf extract were used together at a specific weight ratio, a synergistic action was generated between them, resulting in maximized anti-inflammatory activity in the oral cavity. When a water-soluble plant tincture was added, it was confirmed that the effect was further improved and the present invention was completed.

[extract]

In the present invention, the term "extract" refers to an extract obtained by extraction treatment, a diluted solution or pesticide solution of the extract, a dried product obtained by drying the extract, a purified product or a purified product of the extract, or a mixture thereof, etc. and extracts of all formulations that can be formed using the extract solution. The dried product can be obtained through a conventional process known in the art, such as vacuum drying, freeze drying or spray drying. The purified product undergoes a conventional purification process, for example, membrane separation using a filtration membrane having a cut-off value at a constant molecular weight, separation by various chromatography for separation according to size, charge, hydrophobicity or affinity. can be obtained through

The extract describes each plant described later, and may include its leaves, stems, aerial parts, bark, roots, flowers or flower buds, fruits, seeds, sap, fruits, and whole plants in addition to the specified parts, and may be obtained from plant tissue culture. can also be extracted.

To prepare the extract, one skilled in the art can use any suitable method known in the art. For example, it may be by a solvent extraction method. A solvent extract can be obtained by crushing the whole plant or any part thereof (eg, in a blender) and then treating the extraction solvent. It may be dried after grinding before grinding. In addition, the solvent extract can be prepared in a powder state by additional processes such as distillation under reduced pressure and lyophilization or spray drying. The type of extraction solvent used is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; or mixtures thereof may be used. A solvent extract may be prepared by extracting at least once using the solvent, and a dried extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying may be prepared. The amount of the extraction solvent may vary depending on the type of extraction solvent used, but may be used, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art, such as maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous-alcoholic extraction , countercurrent extraction, microwave-assisted extraction, ultrasonic extraction, supercritical fluid extraction, phytonic extract (eg, hydro-fluoro-carbon solvent), etc.) may be selected and used, and these may be performed alone or Two or more methods may be used in combination.

Centella asiatica extract, corn unsaponifiable extract, and silver leaf extract used as active ingredients of the oral anti-inflammatory composition of the present invention can be used as a water-insoluble anti-inflammatory agent to significantly improve the anti-inflammatory effect of the composition.

Centella asiatica belongs to the family Umbelliferae (Apiaceae), especially the subfamily Hydrocotyle. The active compounds contained in Centella asiatica are, for example, in the form of triterpene genins: asiatic acid and madecassic acid; or pentacyclic triterpenes in the triterpene heteroside form: asiaticoside, madecassoside and terminoloside; and the like. Its origin is Korea, and it is a perennial plant mainly distributed in the islands of the southern region of Korea.

Centella asiatica extract according to the present invention means the result of various formulations obtained through extraction treatment of Centella asiatica. The Centella asiatica extract can be extracted from various organs of natural, hybrid or mutant plants of Centella asiatica, for example, seeds (seeds), roots, aerial parts, stems, leaves, flowers, fruit bodies, It can be extracted not only from the skin of the fruit but also from plant tissue culture, but it can be preferably extracted using the stem or leaf.

The Centella asiatica extract according to the present invention is not particularly limited, but may be preferably performed by a solvent extraction method. A solvent extract can be obtained by treating Centella asiatica with an extraction solvent, and the type of extraction solvent used is not particularly limited, and any solvent known in the art can be used, for example, water, C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol, or mixtures thereof may be used.

The Centella asiatica extract according to the present invention may include various active compounds derived from Centella asiatica, but preferably includes asiaticoside and genin, and the total dry weight of the Centella asiatica extract contains 36 to 36 asiaticoside 44% by weight and 54-66% by weight of total genin (madecassic acid and asiatic acid).

The Centella asiatica extract according to the present invention is insoluble in water, for example, soluble in pyridine, soluble in ethanol, acetic acid, propylene glycol and dioxane, but insoluble in benzene and water. Therefore, in formulations containing excessive amounts of water, for example, in a paste state such as toothpaste, it exists in a suspended state.

Corn unsaponifiable extract (Titrated extract of theunsaponifiable fraction of Zea Mays L.) means a process of quantitatively extracting the unsaponifiable fraction remaining after saponification of corn ( Zea Mays L.). The unsaponifiables are lipid components that are insoluble in water and do not react with strong alkalis to form soluble soap, and include, for example, hydrocarbons, fat-soluble pigments, and fat-soluble vitamins.

The corn used to obtain the unsaponifiable corn extract according to the present invention may be a natural, hybrid or mutant plant, and is not limited to a specific place of origin or distribution.

The corn unsaponifiable extract according to the present invention may include various active compounds derived from corn unsaponifiables, for example, phytosterol, squalene, carotene, topoferol, etc., and the phytosterols include beta-sitosterol and alpha-sitosterol. , or campesterol and the like, preferably beta-sitosterol.

Corn unsaponifiable extract according to the present invention can be obtained through various methods known in the art, and commercially distributed ones can also be used. For example, corn oil collected from corn (eg, corn germ, etc.) is subjected to a continuous saponification process using a caustic soda solution and an organic solvent (eg, n-hexane) to extract unsaponifiables. can be manufactured.

The corn unsaponifiable extract according to the present invention is insoluble in water, and exists in a suspended state in a formulation containing an excessive amount of water, for example, in a paste state such as toothpaste.

Magnolia Bark is Japanese Magnolia ( Magnolia ovobata Thunberg), Magnolia officinalis Rehder et Wilson or Magnolia officinalis Rehder et Wilson var. biloba Rehder et Wilson (Magnolia family Magnoliaceae) ) is the stem bark.

The pumpkin extract according to the present invention means the result of various formulations obtained through extraction treatment of the pumpkin. The zucchini extract can be extracted from various organs of natural, hybrid or mutant plants of zucchini, for example, various organs or parts, i.e., leaves, flowers, fruits, stems, roots, branches and bark, as well as plant tissue culture. Extraction from water is possible, but it can be preferably extracted using the skin.

The pumpkin extract according to the present invention is not particularly limited, but may be preferably performed by a supercritical fluid extraction method. Supercritical fluid extraction is a method of extracting and separating substances using the solubility of a supercritical fluid solvent. When a sample and a supercritical fluid solvent come into close contact with each other, the soluble component in the sample dissolves into the supercritical fluid due to the difference in solubility, The supercritical fluid containing the solute from the extraction step is separated from the solute in the separation step by temperature and/or pressure. The solvent that can be used in the supercritical fluid extraction method according to the present invention is preferably supercritical carbon dioxide, and extraction can be performed under pressure conditions of preferably 30 to 60° C. and/or 10 to 30 mpa. For supercritical fluid extraction, RESS (Rapid Expansion of Supercritical Solutions), GAS (Gas Anti-Solvent), SAS (Supercritical fluid Anti-Solvent), ASES (Aerosol Solvent Extraction System), SEDS (Solution Enhanced Dispersion by Supercritical Fluids), Particles from Gas Saturated Solutions (PGSS) or Reactive Precipitation in Supercritical Solution (RPSS) methods can be used.

Since the silver leaf extract according to the present invention is insoluble in water, it exists in a suspended state in a formulation containing an excessive amount of water, for example, in a paste state such as toothpaste.

The composition according to the present invention contains all three of Centella asiatica extract, corn unsaponifiable extract and silver leaf extract as active ingredients.

In the present specification, "active ingredient" means a component that exhibits the desired activity alone or can exhibit activity in combination with a carrier having no activity itself.

In the present invention, the anti-inflammatory oral composition may include Centella asiatica extract, corn unsaponifiable extract, and silver leaf extract in an amount of 0.003 to 0.5% by weight, more preferably 0.005 to 0.3% by weight, based on the total weight of the composition. can be included with

In the present invention, the Centella asiatica extract may be included in 0.001 to 0.2% by weight, preferably 0.005 to 0.1% by weight, based on the total weight of the composition. In addition, the corn unsaponifiable extract may be included in an amount of 0.001 to 0.2% by weight, preferably 0.005 to 0.1% by weight. In addition, the silver leaf extract may be included in an amount of 0.001 to 0.2% by weight, preferably 0.005 to 0.1% by weight.

In the present invention, the anti-inflammatory oral composition may include at least two of Centella asiatica extract, corn unsaponifiable extract and silver leaf extract as an active ingredient. In the case where the active ingredient is Centella asiatica extract and Sulfur leaf extract, a weight ratio of 1: 1 to 20, preferably 1: 2 to 10, and more preferably 1: 3 to 6 (Centella asiatica extract: Sulfur extract) may be there is. In addition, when the active ingredient is Centella asiatica extract and corn unsaponifiable extract, the weight ratio of 1: 0.5 to 10, preferably 1: 1 to 7, more preferably 1: 2 to 5 (Centella asiatica extract: corn unsaponifiable extract). In addition, when the active ingredient is corn unsaponifiable extract and silver leaf extract, a weight ratio of 1: 0.5 to 20, preferably 1: 1 to 10, more preferably 1: 2 to 5 (corn unsaponifiable extract: silver leaf extract) may be there is.

In the present invention, when the active ingredient is composed of Centella asiatica extract, corn unsaponifiable extract, and silver leaf extract, the weight ratio of Centella asiatica extract, corn unsaponifiable extract, and silver leaf extract is 1: 0.5 to 10: 1 to 20. , preferably 1: 1 to 7: 2 to 10, more preferably 1: 2 to 5: 3 to 6, most preferably 1: 2: 5 (Centella asiatica extract: corn unsaponifiable extract: Sulfur extract) can

Preferably, the anti-inflammatory oral composition of the present invention can exhibit the most excellent anti-inflammatory activity when the active ingredient is included in the above content or weight ratio range.

[Plant tincture agent]

In addition to the three extracts described above, the composition according to the present invention may further include a water-soluble plant tincture.

The plant tincture refers to a medicinal preparation made by leaching plants with a mixed solution of ethanol and purified water.

As the water-soluble plant tincture according to the present invention, various water-soluble plant tinctures commonly used in the art may be used within the scope of not impairing the effects of the present invention, but are not limited thereto, preferably myrrh tinc, ratania tinc, and camo. Milletink and mixtures of two or more thereof may be used.

The myrrh tinc is a tincture agent using myrrh, and the myrrh is a bitter, fragrant, yellowish reddish-brown oleoresin rubber, from a small, thorny flowering tree of the genus Commiphora or It is obtained from plants of the Burseraceae family, such as Commiphora myrrha and Commiphora abyssinica, but is a substance distinct from the mentioned plants (Commiphora myrrha). The ratania tincture is a tincture agent using ratania (rhatany), and the ratania is a plant belonging to the Krameria caseae family and is native to Ecuador, Peru, and Bolivia. It grows wild on the western slopes of the Andes Mountains at an altitude of 1,000m to 3,000m above sea level. Ratania is usually used dry roots. The chamomile tincture is a tincture agent using chamomile, and the chamomile (chamomilla) is also referred to as bundae chrysanthemum, and is native to Europe. The stem is branched a lot at the bottom, is 50cm to 100cm high, has an apple scent, and has angular lines. In Europe, it was taken internally for neuralgia, toothache, dysmenorrhea, and used for stomatitis, conjunctivitis, and suppuration in the private sector. Chamomile is used as an anti-inflammatory, anticonvulsant, carminative, and antiseptic, and dried flower buds are used. The main ingredients are Chamazulene, α-bisabolol oxide A, α-bisabolol oxide B, and bisabolone oxide A.

The plant tincture may use a commercially distributed product or may be prepared according to various methods known in the art, for example, a maceration method or a percolation method may be used. The cold soaking method is a method of leaching soluble components by immersing plants in a leachate at room temperature, and the percolation method is a method of leaching soluble components by passing a leachate through a plant layer filled in a percolator.

In the composition according to the present invention, the water-soluble plant tincture may be included in 0.005 to 3% by weight, preferably 0.01 to 2% by weight, more preferably 0.02 to 1.5% by weight based on the total weight of the composition. When the water-soluble plant tincture is included in the range of 0.005 to 3% by weight, a particularly excellent effect can be exhibited in combination with the three kinds of extracts.

In the composition according to the present invention, the weight ratio of the water-soluble plant tincture and the three extracts may be 1: 0.01 to 100, preferably 1: 0.1 to 50 (plant tincture: extract), and 1: 0.5 to 30 In the range, a particularly excellent effect can be exerted.

[Additional Ingredients]

In the composition according to the present invention, within the range of not impairing the effect of the present invention, known additives that can be used in oral compositions are blended for proper formulation and formulation stability, enhancement of desired effects, enhancement of preference for use, etc. can do. Examples of such additive components include abrasives, wetting agents, binders, foaming agents, sweeteners, pH adjusters, caries preventive agents, fragrances, solvents, and desensitizing agents. Although specific examples of the additive component have been described below, the components that can be incorporated into the oral composition of the present invention are not limited thereto.

Examples of the abrasive include silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate, and aluminum silicate. There is, but is not limited thereto, preferably silica may be used. The abrasive may be included in an amount of 1 to 20% by weight based on the total weight.

As the wetting agent, for example, concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycols, propylene glycol, etc. may be used, but is not limited thereto.

Examples of the binder include sodium carboxymethylcellulose, carrageenan, xanthan gum, hydroxyethylcellulose, hydroxypropylmethylcellulose, hydroxypropylcellulose, guar gum, gellan gum, carbomer, pectin, polyvinylpyrrolidone , carboxyvinyl poly, and at least one selected from the group consisting of sodium alginate and laponite, preferably sodium carboxymethylcellulose or xanthan gum, etc. may be used, but is not limited thereto.

Examples of the foaming agent include anionic and nonionic surfactants such as sodium alkyl sulfate, sodium lauryl sulfate, sucrose fatty acid ester, and sorbitan fatty acid ester, but are not limited thereto.

The sweetener may be, for example, sodium saccharin, sucralose, maltitol, aspartame, erythritol, xylitol, and licorice acid, but is not limited thereto.

As the preservative, benzoic acid, methyl paraben, propyl paraben, paraoxybenzoic acid ester, and sodium benzoate may be used, but are not limited thereto.

Sodium fluoride, sodium monofluorophosphate, tin fluoride, chlorohexidine, allantoin chlorohydroxyaluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopherol acetate, enzymes, etc. may be used as the anti-cavity agent. Not limited.

As the fragrance, peppermint oil, spearmint oil, menthol, and the like may be used alone or in combination, but are not limited thereto.

As the pH adjusting agent, sodium phosphate, disodium phosphate, citric acid, triethanolamine, etc. may be used, but is not limited thereto.

Examples of the desensitizing agent include fluorides such as sodium fluoride, potassium fluoride, ammonium fluoride, tin fluoride, sodium monofluorophosphate, enzymes such as amylase, protease, lysozyme, and dextranase, vitamins B, C, E, etc. Of vitamins, potassium nitrate, aluminum lactate, etc. may be used, but is not limited thereto. The desensitizing agent may contain 0.01 to 5% by weight based on the total weight.

Moreover, as said solvent, water, a C1-C4 lower alcohol, etc. are mentioned, for example. When the composition for oral cavity contains water as a solvent, its content may be included as 20 to 95% by weight relative to the total weight of the composition for oral cavity. When a composition for oral cavity contains a lower alcohol as a solvent, the content may be 1 to 30 weight% with respect to the whole composition for oral cavity.

In addition to the above-mentioned additive components, natural materials known in the art may be further included within a range that does not impair the effects of the present invention. For example, cnidium extract, Rehmannia Root extract, Angelica gigas extract, or a mixture thereof may be included, but is not limited thereto. The content may be 0.001 to 1% by weight of each extract relative to the total weight of the oral composition.

[composition]

The dosage form and shape of the composition for oral cavity according to the present invention are not particularly limited, and examples thereof include liquid (solution, emulsion, suspension, etc.), semi-solid (gel, cream, paste, etc.), solid (tablet, granular agent, capsule agent). , film agent, kneaded product, molten solid, waxy solid, elastic solid, etc.).

In addition, the prepared preparations, for example, dentifrices (soft skirts, liquid skirts, liquid skirts, powdered skirts, etc.), mouthwashes, liniments, patches, oral fresheners, foods (eg, chewing gum, chewing gum, and confectionery products) , candy, gummies, films, troches, etc.), but is not limited to the above in the range of oral use.

[Efficacy/Effect]

The oral composition according to the present invention exhibits excellent anti-inflammatory activity.

The combination of Centella asiatica extract, corn unsaponifiable extract, and silver leaf extract exhibits a synergistic effect that exceeds the sum of the effects of the plants as starting materials of each extract, their intermediates, and their final materials.

The anti-inflammatory oral composition according to the present invention has excellent antibacterial activity as well as anti-inflammatory properties.

The oral composition according to the present invention has an effect of improving various diseases, disorders or harmful symptoms that may occur in the oral cavity based on its excellent anti-inflammatory or antibacterial activity.

In the present specification, "improvement" means treatment, prevention, and alleviation, and the "prevention" may be understood as a comprehensive concept of preventing a disease or the like before it occurs. The "relief" means that a disease or the like is reduced or mitigated, and can be understood as a broad concept including improvement, prevention, treatment, and the like. "Treatment" can be understood as a broad concept meaning restoring a disease or the like to be close to an original state in which it did not occur.

The oral composition according to the present invention is particularly effective in improving periodontal disease.

The periodontal disease is divided into gingivitis and periodontitis according to the severity of the disease. Gingivitis is a relatively light and fast-recovery form of periodontal disease that has lesions limited to soft tissues such as the gums. When the inflammation caused by gingivitis spreads to the gums and surrounding bone, it is called periodontitis. This periodontal disease progresses as proteins present in saliva in the oral cavity form a thin film on the surface of the teeth, and bacteria adhere to and proliferate along with food, exfoliated epidermal cells, and white blood cells. Bacteria deposit together with inorganic substances to create plaque and release toxins such as LPS (Lipopolysaccharide), nuclease, leukotoxin, and hydrogen sulfide, causing an inflammatory reaction in the surrounding tissues to form periodontal pockets between teeth and gums. Various types of cytokines are secreted from host immune cells, and MMP (Matrix metalloproteinase) is secreted by host immune cells and bacteria. As the inflammatory reaction intensifies, the depth of the periodontal pocket deepens, causing gingival recession and bone loss. periodontal disease progresses. The main anaerobic bacteria causing the periodontal disease include Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Treponema denticola, and Privotella intermedia (Prevotella intermedia), etc.

[Manufacturing method]

The composition according to the present invention can be prepared according to a conventional method known in the art. Specifically, powder components such as sodium carboxymethyl cellulose and saccharin are dispersed in sorbitol as a wet component, purified water is added, and mixed first in a mixer, and then an abrasive such as precipitated silica and a medicinal agent are added and mixed. After, finally, a composition for oral cavity may be prepared by adding a flavoring component and mixing under vacuum.

According to the present invention, there is an excellent effect that the anti-inflammatory effect in the oral cavity is maximized, and furthermore, the effect of improving periodontal disease is maximized.

Hereinafter, examples and the like will be described in detail to aid understanding of the present invention. However, the embodiments according to the present invention can be modified in many different forms, and the scope of the present invention should not be construed as being limited to the following examples. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example : Preparation of oral composition

Toothpaste compositions of Examples 1 to 4 and Comparative Examples 1 to 4 were prepared with the ingredients and composition ratios shown in Table 1 below. Specifically, powder components such as sodium carboxymethyl cellulose and saccharin were dispersed in sorbitol as a wet component, purified water was added, and mixed first in a mixer, then abrasives such as precipitated silica and medicinal agents were added and mixed. . Finally, a toothpaste composition was prepared with the ingredients and composition ratios shown in Table 1 below by adding the perfume ingredients and mixing under vacuum.

division Example comparative example One 2 3 4 One 2 3 4 dental type silica 14.000 14.000 14.000 14.000 14.000 14.000 14.000 14.000 Sorbitol solution (70%) 50.000 50.000 50.000 50.000 50.000 50.000 50.000 50.000 sodium saccharin 0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100 carboxymethylcellulose 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 Spices 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 sodium lauryl sulfate 2.000 2.000 2.000 2.000 2.000 2.000 2.000 2.000 Purified water Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Zucchini Extract 0.001 0.050 0.100 0.200 - 0.001 - - Centella asiatica extract 0.001 0.010 0.020 0.050 - - 0.001 - Corn Unsaponifiable Quantitative Extract 0.001 0.020 0.040 0.050 - - - 0.001

Toothpaste compositions of Examples 5 to 19 were prepared with the ingredients and composition ratios shown in Table 2 below.

Experimental example : water insoluble Composition ratio of anti-inflammatory agent (vegetable extract) and Ting Gingival bleeding inhibitory effect according to the content

<Experiment method>

The criteria for the tooth and tooth surface, which are the subject of the study, were applied in the same way as for the gingivitis index evaluation. In the gingivitis examination, 1 point was scored if there was bleeding within 10 to 30 seconds after probing, and 0 points if there was no bleeding (Bleeding on probing, BOP), and the incidence of bleeding on the entire test surface was calculated.

BOP = sum of total scores / total number of teeth inspected X 100

<Experiment result>

The experimental results are shown in Table 3 below. As shown in Examples 1 to 4, it was confirmed that the gingival bleeding index decreased according to the content and ratio of the water-insoluble anti-inflammatory agent. In particular, when the weight ratio of silver leaf extract, Centella asiatica extract, and corn unsaponifiable extract is 5:1:2 (Silver leaf extract: Centella asiatica extract: corn unsaponifiable extract) compared to 1:1:1, gingival bleeding It can be seen that the effect of reducing the index is the highest, and a synergistic effect on the anti-inflammatory effect appears at a specific ratio. In Examples 5 to 19, the gingival bleeding index tended to decrease depending on the content and type of tincture, and when the three types were used in combination, the reduction in the gingival bleeding index was more marked than in the group used alone. Confirmed. Therefore, it was confirmed that the reduction effect of the bleeding index by the oral hygiene agent can be further improved by jointly using the water-insoluble anti-inflammatory agent and the tincture agent.

toothpaste group n Baseline 4 weeks later P-value average Standard Deviation average Standard Deviation Example 1 30 8.82 2.22 6.82 1.82 Initial contrast<0.001 Example 2 30 8.81 1.89 6.31 1.77 Example 3 31 8.86 1.85 5.86 2.10 Example 4 29 8.88 1.71 5.48 1.52 Example 5 29 8.81 1.73 5.31 1.64 Example 6 30 8.87 1.64 4.87 1.82 Example 7 30 8.86 1.73 4.56 1.54 Example 8 31 8.83 1.84 4.23 1.93 Example 9 31 8.86 1.68 5.36 1.90 Example 10 30 8.86 1.67 4.86 2.15 Example 11 30 8.85 1.95 4.55 1.54 Example 12 30 8.81 1.69 4.21 2.25 Example 13 29 8.80 1.92 5.30 1.95 Example 14 31 8.86 1.93 4.86 1.73 Example 15 31 8.83 1.51 4.53 1.80 Example 16 30 8.85 1.51 4.25 1.55 Example 17 30 8.83 1.51 4.63 1.51 Example 18 30 8.81 1.92 4.61 1.60 Example 19 29 8.84 1.52 4.34 1.90 Comparative Example 1 29 8.83 2.00 8.53 1.61 Comparative Example 2 31 8.88 1.85 8.38 2.11 Comparative Example 3 29 8.81 2.09 8.31 1.72 Comparative Example 4 30 8.82 1.82 8.32 2.22

Claims (8)

An oral composition for preventing or treating periodontal disease comprising Centella asiatica extract, corn unsaponifiable extract and silver leaf extract as active ingredients. The oral composition for preventing or treating periodontal disease according to claim 1, wherein the Centella asiatica extract, corn unsaponifiable extract and silver leaf extract are present in an amount of 0.003 to 0.5% by weight based on the total weight of the composition. The method of claim 1, wherein the weight ratio of the Centella asiatica extract, the corn unsaponifiable extract and the Sulfur extract is 1: 0.5 to 10: 1 to 20 (Centella asiatica extract: corn unsaponifiable extract: Sulfur extract). Oral composition for preventing or treating periodontal disease. The oral composition for preventing or treating periodontal disease according to claim 1, wherein the Centella asiatica extract is a solvent extract using water, lower alcohol, or a mixture thereof as a solvent. The oral composition for preventing or treating periodontal disease according to claim 1, wherein the zucchini extract is a carbon dioxide supercritical extract. The oral composition for preventing or treating periodontal disease according to claim 1, wherein the composition further comprises a water-soluble plant tincture. The oral composition for preventing or treating periodontal disease according to claim 6, wherein the water-soluble plant tincture is at least one selected from the group consisting of myrrh tincture, ratania tincture, and chamomile tincture. The oral composition for preventing or treating periodontal disease according to claim 6, wherein the water-soluble plant tincture is 0.005 to 3% by weight based on the total weight of the composition.