KR940006065B1 - Dentifrice compositions for using carious tooth tooth root disease - Google Patents

Abstract

An oral composition for the prevention of dental caries and gingival diseases contains 0.01-3 wt.% of plant extracts from myrrha (or myrrh oil), mori cortex, cimicifuga and green tea. The composition has inhibitory activity against streptococcus mutans, Actinomyces viscous and Bacteroides gingvalis and also inhibitory effect of glucosyl transferase. The extraction solvent is H2O, methanol, ethanol or aceton. Pref. formulation of the oral composition is tooth paste, oral cleaning agent, candy or chewing gum.

Description

[Name of invention]

Toothpaste composition effective for caries and periodontal disease

Detailed description of the invention

The present invention relates to a toothpaste composition comprising a extract from myrrh oil (or Myrrh Oil), lettuce, horse riding and green tea, and more specifically, the causative agent of Streptococcus mutans (ATCC 27607, ATCC 27351, ATCC 25175, NRRL 10449), Streptococcus sanguis, Streptococcus salivarius> and actinomycetes viscous, Actinomyces naeslundi, Bacteroides gingvalis, Bacteroides forsythus, Bacteroides intermedius, and antibacterial glucosyl glucose glucosyltransase (Glucose gluase) It relates to a composition for oral cavity, in particular toothpaste composition for preventing tooth decay and periodontal disease caused by plaque formation by inhibiting.

In general, tooth decay is caused by various kinds of oral bacteria to produce cohesive polysaccharides, so that each bacteria easily adheres to the tooth surface, and bacteria form the plaque, Bacteria break down sugars to produce acids, which demineralize the tooth's enamel surface to advance tooth decay. As plaque further progresses to calculus, it causes gingivitis, gingivitis, and periodontal disease of alveolar pylori.

It is reported that there are various side effects when using antibiotics, fungicides, etc. as a method for preventing such oral diseases. Dent. Res., 25, 441 (1946), J. Dent. Res., 31, 421 (1966), J. Oral Ther. Pharmacol., 3, 157 (1966), J. Am. Dent. Ass., 87 1006 (1973)>.

Therefore, the present inventors have investigated the antibacterial and plaque formation inhibitory effects of various plant extracts. As a result, the mixed plant extracts of myrrh, baekryepi, horseback riding, and green tea are known to be used as the conventional plant extracts against caries and periodontal disease. 134729 (A) (Liquorice, Morus bark), JP 88-253019 (A) (Pepper Sprouts), JP 83-39614 (A) (Salvia, Rosemary)> showed superior antibacterial effects and played an important role in plaque formation. Existing Plant Extracts for the Extracellular Enzyme GTase of S. mutans <JP Hei 1-90624 (A) (Green Tea, Oolong Tea, Black Tea), JP 87-48616 (A) (Mint, Rosemary), JP 87- It was confirmed that 58331 (B ₂ ) (Senega, hazel) has a superior GTase inhibitory effect. When the mixed plant extracts are formulated in various oral compositions, they show antimicrobial and GTase inhibitory effects on caries and periodontal disease-causing bacteria, thereby finding that oral compositions for the purpose of preventing caries and periodontal disease can be obtained. Was completed.

Myrrh is Commphora molmol Engl. It is a rubber resin obtained from wounds of the bark of the same plant (Oliveaceae Burseraceae). It has been used for thrush, gingivitis and oral detergent due to its analgesic and antiseptic effect, and the cedar is the root of Morus alba L. or the same plant (Moraceae). The shell has a blood pressure drop, anti-inflammatory, and tooth pain. Horseback riding is a root stem of Cimicifuga heracleifolia komarov or other similar plants (Muniaceae, Ranunculaceae). It has been widely used in prescription of Chinese medicine for toothache and oral inflammation due to analgesic and antipyretic effects. Green tea has been used as a favorite product since ancient times as a leaf of Camellia sinensis and the same plant. Professor Masao of the Tokyo University School of Dentistry has taught that 300 children of the national school had children who had tea after feeding, compared to children who did not drink tea. A report suggesting the relationship between car and tooth decay has been published, demonstrating that tea has antimicrobial activity against caries (Fragrance J., p48 (1990)), and tea also has the effect of eliminating bad breath in the mouth. Fragrance J., p 37 (1990).

Hereinafter, the present invention will be described in detail.

The present invention relates to the combination of extracts derived from myrrh oil (or Myrrh Oil), baekryepi, horse riding, green tea in various oral compositions such as toothpaste, mouthwash, candy and chewing gum, and particularly effective in toothpaste compositions effective against caries and periodontal disease. It is about. The solvent used for the extraction of the plant was extracted by adding the extraction solvent 2-10 times with respect to the plant material with a polar solvent such as water, methanol, ethanol, acetone, etc., and the filtrate was concentrated to dryness to obtain a concentrate. And the suspension which melt | dissolved this concentrate with the suitable solvent can also be used. The mixed plant extracts thus obtained are applied to various types of products and applied in the oral cavity to prevent tooth decay and periodontal disease. It is preferable that the compounding amount of the plant extract solution to the oral composition is 0.001-10% (w / w), especially 0.01-3% (w / w), of the total, and various toothpastes are applicable as an oral composition that can be applied. , Mouthwashes, candies and chewing gum.

Various oral compositions for carrying out the present invention should be prepared using suitable ingredients according to the type thereof, for example, in the case of toothpaste, dicalcium phosphate, calcium carbonate, calcium pyrophosphate, calcium metaphosphate, silicas, Abrasives (0.4-10%) such as aluminum silicate, aluminum hydroxide, carboxymethyl cellulose, hydroxymethyl cellulose, alginate, carrageenan, gum gum, polyvinyl alcohol, etc. Humectants such as sorbitol, glycerin polyvinyl alcohol, sodium lauryl sulfate, sodium lauryl sarcosinate, monoglyceride sulfate, sucrose fatty acid ester, polyoxyethylene hardened castor oil, polyoxyethylene polyoxypropylene copolymer, polyoxyethylene In addition to foaming agents such as sorbitan fatty acid esters, water, ingredients such as flavoring agents, saccharin, stevioside, sweeteners, and preservatives Mixture will be prepared according to conventional toothpaste preparation. Toothpaste prepared by the addition of plant extracts according to the present invention include sodium fluoride, sodium monofluorophosphate, salt, allantoin, pyrophosphate, aminocaroroic acid, vitamins, doncrorophyll sodium, tranesamic acid, zinc chloride, triclosan Also, various active ingredients commonly used in toothpaste, such as cetylpyridium chloride and vinyl methyl ether / maleic acid copolymer, can be added. In addition, products such as mouthwashes, candies and chewing gum can be prepared by appropriately blending various ingredients according to the manufacturing method.

Representative examples of extract preparation of the present invention are as follows.

Extract Preparation Example

To 50 g of the dried herbal medicine (green tea) was added 500 ml of 70% ethanol, which was immersed at room temperature for 10 days, and filtered. The filtrate was concentrated under reduced pressure to give 20.02 g of a concentrate. Other dry herbal medicines (myrrh, zinnia, horseback) were extracted according to the method described above to give a concentrate of 6.1 g, 5.2 g and 4.8 g, respectively. Then, 70% ethanol was added to these concentrates to obtain an extract having a concentration of 50% (w / w).

Example 1.

[Antibacterial Activity Measurement in Toothpaste Base]

1) Preparation of the Composition

Toothpaste of the following formulation was prepared according to the conventional method of formulating the herbal extract prepared according to the method of extract preparation example.

2) Experiment Method

10 g of each toothpaste prepared according to the prescription was added to 10 ml of Brain Hert Infusion Broth (BHI) containing 2% sugar, completely dispersed, diluted with BHI broth to 0.06-0.18% of toothpaste concentration, S. mutans (ATCC 25175) and periodontal disease-causing bacteria (Actinomyces vicosus); ATCC 15987) was inoculated and incubated for 24 hours in a 37 ° C. incubator. After incubation, the antimicrobial activity of the two types of bacteria according to the toothpaste concentration and the herbal extract concentration was obtained as shown in Table 1, 2.

TABLE 1

Antimicrobial Effects of S. mutans (ATCC 25175) by Plant Extracts in Toothpaste Base

(+: Growth of bacteria,-: non-growth of bacteria)

TABLE 2

Antimicrobial Effect of Periodontal Disease-causing Bacteria (Actinomyces viscosus; ATCC 1578) by Plant Extracts in Toothpaste Base

(+: Growth of bacteria,-: non-growth of bacteria)

Sodium lauryl sulfate is a foaming agent in toothpaste, 1-2% of the total content in the toothpaste, which itself is known to have some antimicrobial activity. As shown in Tables 1 and 2, when the mixed extract of myrrh, baekryepi, horse riding, and green tea was blended in the toothpaste composition, the antimicrobial activity was much higher than that of sodium lauryl sulfate alone.

Example 2.

[Measurement of GTase Inhibitory Effect in Toothpaste Base]

1) Preparation of the Composition

The composition was prepared in the same manner as in Experimental Example 1, and used in the experiment.

2) Experiment Method

① GTase Separation and Purification

Streptococcus mutans ATCC 25175 was inoculated in 3 liters of BHI broth and incubated for 18 hours in a 37 ° C incubator, followed by centrifugation (3000 rpm for 20 minutes) to remove the cells, and the supernatant was saturated with ammonium sulfate 60-70%. After making it stand, it was left to stand at 4 degreeC for 18 hours. The precipitate thus produced was centrifuged (3000 rpm, 20 minutes), dissolved in 200 ml of potassium phosphate buffer (0.05 M, pH 6.5), and dialyzed with the same buffer for 24 hours. The dialysis solution was centrifuged again to remove insoluble matters, and the obtained coenzyme solution was injected into a column filled with hydroxyapatite, and the same buffer solution having different ionic strengths was eluted step by step to purify purified Gtase. Got it.

② Measurement of GTase Inhibition Activity

4 g of 0.05 M potassium phosphate buffer solution (pH 6.5) containing 50 to 100 μg of GTF prepared by the above method, 70 μg of sugar and 0.02% NaN ₃ , each of 10 g of the toothpaste prepared according to the above formulation was used as distilled water. After diluting to%, centrifugation (3000 rpm, 20 minutes) was carried out to obtain a supernatant. The solution was rediluted to a toothpaste concentration of 0.67 and 1.5%, then added to a buffer solution and allowed to react with an incubator at 37 ° C. for 18 hours. The amount of glucan produced was measured as absorbance (660 nm) in a spectrophotometer. The decrease in absorbance at this wavelength is proportional to the inhibition of glucan production, ie GTase inhibition. The GTase inhibitory activity was determined by the following equation. The absorbance of the control solution was that of the reaction solution except the plant extract.

The result shown in Table 3 was obtained by the said method.

TABLE 3

GTase Inhibitory Effect of Plant Extracts on Toothpaste Base

Sodium lauryl sulfate in toothpaste has a slight GTase inhibitory effect, but it is not satisfactory to prevent tooth decay, so it is necessary to add another GTase inhibitor. As shown in Table 3, when toothpaste was formulated with plant extracts such as myrrh, baekryepi, horse riding, and green tea on the prescription, it showed a much better effect than when sodium lauryl sulfate was contained. The inhibitory effect is expected to have caries prevention effect.

As shown in the results of Table 1-3, when the mixed tooth extracts of myrrh, lettuce, horseback riding and green tea were mixed in the toothpaste prescription, they were much better than the toothpaste and periodontal pathogens compared to the toothpaste without the mixed plant extracts. It showed antimicrobial and GTase inhibitory effects. Therefore, the mixed plant extracts according to the present invention can expect excellent caries and periodontal disease prevention effect.

Specific embodiments of the present invention are as follows.

Example 1

Example 2

Example 3

Example 4

Example 5

Example 6

Example 7

Example 8

Example 9

Claims (1)

Toothpaste composition characterized in that it contains a mixture of extracts obtained by extracting myrrh, baekryepi, horse riding and green tea with water, alcohol or acetone in an amount of 0.001-10% (w / w).