JPH0253717A - Dentifrice or mouth wash - Google Patents

Abstract

PURPOSE:To obtain a mouth wash having inhibiting effect against the activity of hyaluronidase called as phlogogenic enzyme participating in inflammation and useful as a preventive and remedy for periodontal diseases by using extract and/or powder of a plant such as JIYU (root of Sanguisorba officinalis), EIJITSU (fruit of Rosa multiflora), black tea, green tea and camellia as active component. CONSTITUTION:The objective dentifrice contains extract and/or powder of one or more kinds of plants selected from JIYU, EIJITSU, black tea, green tea, camellia and KINMA (Peper betle). The active component is compounded with an abrasive, humectant, surfactant and preservative to obtain the dentifrice, etc. The amount of the active component in the mouthwash or dentifrice is preferably about >=0.003% in terms of the original plant before extraction. JIYU is rhizome of burnet, a plant of family Rosaceae and EIJITSU is fruit of briar, a plant of family Rosaceae. KINMA is dried fruit cluster of Peper betle, a plant of family Piperaceae.

Description

Detailed Description of the Invention [Industrial Application Field] The present invention provides plant extracts and plant extracts that have the effect of inhibiting the activity of hyaluronidase (hereinafter abbreviated as Hase), which is also called a inflammatory enzyme and is involved in the development of inflammation. The present invention relates to a toothpaste or mouthwash that is effective in preventing and treating periodontal diseases and is characterized by containing powders of these plants.

[Prior art and problems to be solved by the invention] The mechanism by which dental caries occurs is that bacteria in the oral cavity, especially Streptococcus mutans, convert food-derived m-saccharide into a highly adhesive polysaccharide glucan, which is then transferred to the tooth surface. It adheres to the teeth and forms plaque. Bacteria then produce organic acids such as lactic acid within this plaque, which is believed to demineralize tooth enamel.

Based on this process of dental caries development, bactericidal agents are used to prevent Streptococcus minitans, antienzyme agents are used to prevent glucosyltransferase, an enzyme involved in glucan production, and enzyme dextrin is used to inhibit glucan production or prevent adhesion to tooth surfaces. Stranase has been applied.

On the other hand, so-called periodontal diseases such as alveolar pyorrhea and gingivitis have become such an important problem that the Ministry of Health and Welfare recently announced that measures will be taken to prevent them as an adult disease. Currently, the cause of dental caries has not been investigated.

The present inventors focused on the following with the intention of developing preventive and therapeutic agents for periodontal diseases from a new perspective. That is,
■Recently, it has been revealed that the onset of allergies is triggered by an antigen-antibody reaction, which activates Iase in mast cells and releases excessive histamine, which is a mesoieator of inflammation. , the creation of anti-inflammatory agents and anti-allergic agents is progressing from the perspective of Hase inhibitors; ■ Extracellular enzyme Ba5e of the bacterial group contained in dental plaque increases the permeability of the gum epithelium, leading to the development of gingivitis. and ■ Hyaluronic acid contained in cement, the substance that adheres the cells of the gum epithelium, is decomposed by Ba5e and the ζ cell gap expands - a process stimulated by bacterial proliferation and plaque formation, leading to gingivitis. We focused on the occurrence of

Based on the above viewpoint, the present inventors conducted repeated research in search of Hase inhibitors in a new field, and as a result, they discovered that there are substances that inhibit Hase in plant extracts that have been used for other purposes. Heading The invention has been completed.

That is, an object of the present invention is to provide a toothpaste or mouthwash containing a plant extract having the effect of inhibiting periodontal diseases, thereby contributing to the prevention and treatment of periodontal diseases.

In addition, it has been found that among the plant extracts of the present invention, the extracts of the plant extract have an astringent effect, and the extract of the extract of the present invention has an antibacterial effect, and in this respect, they are suitable for the prevention and treatment of periodontal diseases. .

[Means for Solving the Problems] The plant extract and/or plant powder used in the present invention is selected from 1f! ! Or it is based on two or more types. These can be used in combination as appropriate.

In addition, unless otherwise specified, the above-mentioned plant extract means a plant extract obtained by the method described below, a diluted solution thereof, a concentrated extract thereof, and a dried powder, but in the present invention, these original plants are dried and powdered. A converted bulk powder can also be used. The reason for this is that the toothpaste or mouthrinse targeted by the present invention contains water, alcohol, and moisturizing agents such as 1,3-butylene glycol, polyethylene glycol, and glycerin, so it is difficult to use during the manufacturing process, storage, or use. The active ingredients are extracted from saliva.

For the extraction of the plant extract used in the present invention, water, lower-hydric alcohols such as methyl alcohol, ethyl alcohol, and isopropyl alcohol that can be mixed with water, propylene glycol, 1,3-butylene glycol, hexylene glycol, and polyethylene glycol are used. 1f of glycols such as, organic solvents such as glycerin! Alternatively, two or more types can be used in appropriate combination. Further, the extraction temperature is not particularly limited, and may be high temperature, room temperature, or low temperature.

Both jiyu and jitsu have been used as herbal medicines since ancient times, and betel nut has been used as a spice.
Black tea and green tea have also been served for drinking since ancient times.
Therefore, its safety for the human body is extremely high.

Jyu is a ground elm, and it is more preferable to use the roots or rhizomes of Porphyra and its varieties, which belong to the Rosaceae family.

It is a commercial fruit, and it is preferable to use false fruits or fruits of Rosaceae or its related plants belonging to the Rosaceae family.

Black tea and green tea are both based on tea of the Camellia family, and the former is fermented and the latter is fermented, but it is preferable to use young leaves of each. Additionally, camellia leaves can also be used in the same manner. In addition, fresh leaves of tea and camellia can also be used.

It is preferable to use betel by drying the hook part of the betel, which belongs to the Betelinaceae family.

In the present invention, extracts or powders of these plants can be used alone or in a mixture of two or more, or can be used after being appropriately formulated.

If you use these in your toothpaste or mouthwash,
It becomes possible to prevent or cure periodontal disease by inactivating e.

The abrasives, humectants, binders, surfactants, preservatives, medicinal ingredients, etc. used in manufacturing the toothpaste or mouthwash of the present invention are those used in ordinary toothpastes or mouthwashes. It may be appropriately selected depending on the dosage form and is not particularly limited.

The amount of the plant extract or powder in the toothpaste or mouthwash of the present invention varies depending on the dosage form, but it is usually desirable that it be about 0.003 or more when converted to the original plant before extraction.

[Example] Hereinafter, the present invention will be specifically explained with reference to Examples.

(Example 1 Preparation of Plant Extract) - Extract of 100 g of roots of C. chinensis with 800 m of 50% ethyl alcohol, cooled, and filtered. The filtrate was evaporated to dryness under reduced pressure, and the residue was dissolved in 8001 nm of water. Dissolve by heating.

After that, the insoluble matter is filtered off, and the filtrate is concentrated under reduced pressure. 5 residues
It was dissolved in 0λ ethyl alcohol and the total amount was adjusted to 300m.

・Add 100 g of fruit powder of Eizu extract to 500 mff1 of 5096-ethyl alcohol, heat extract, cool, and filter. The filtrate was concentrated under reduced pressure, and the residue was dissolved in 50% ethyl alcohol to adjust the total volume to 200 ml.

- Black tea extract Slightly dried Arasamcha leaves are thoroughly rubbed and fermented, then dried at 85-90°C. 50 for 10g of this strip
Add 100 ml of zero ethyl alcohol and extract at room temperature for 5 days, then cool and filter. 60 zeros on this night.
50 ml of 3-butylene glycol was added and filtered again.

・Green tea extract Steam young tea leaves with steam, rub well and dry. The strips were treated with a solvent according to the method for producing black tea extract described above.

- Camellia extract Fresh camellia leaf strips were treated with a solvent according to the method for producing black tea extract described above.

・Betel extract 10g of dried betel fructose strips 50% 1.3-
The mixture was added to 40 g of butylene glycol, extracted at room temperature for 2 days, and then filtered.

(Example 2 Ha se inactivation effect of plant extract) Ha
The inactivation effect of se is based on the phenomenon that the viscosity of a hot solution of HA decreases due to enzymatic decomposition. It was tested by measuring over time using

(Test method) ■ In the system to which the extract obtained in Example 1 was added, a mixed solution consisting of the following substrate solution (7 mu), enzyme solution (1 company), and extract solution (1 company) was heated at 37°C. The viscosity change was measured over time. On the other hand, ■ In the system without the addition of extract (control), o, 1 molar phosphate buffer (inININ) was added instead of the extract solution.
) was added to the 3-component mixture, and the viscosity change was measured in the same manner as in (2) above.

Substrate solution: Sodium hyaluronate (manufactured by Kewpie Co., Ltd., molecular weight 1,000,000 to 1,200,000) was dissolved in 0.1 molar phosphate buffer (pH
6,0) to prepare a 0.496 solution.

・Enzyme solution: Ushigomaru hyaluronidase (manufactured by Sigma, type is) 0.1 molar phosphate buffer (pH 6.0) to prepare a 0.001% solution.

・Extract solution: 0 of the plant extract produced in Example 1 above.
．． A solution diluted with 1 molar phosphate M solution (pHe, o), diluted 30 times for juniper, 10 times for age, 100 times for black and green tea, and 5 times for camellia and betel.

The relative viscosity of each solution at the start of measurement was set as 1, and the ratio of relative viscosity at each reaction time was calculated. The results are shown in the table. The numbers in parentheses in the table are )(stabilization rate of A, i.e.
It represents the effect of controlling and stabilizing the decomposition of l(A) by 1(ase), and is a number calculated using the following formula based on the relative viscosity at each reaction time.

Above, test results using several types of plant extracts have been illustrated.

(Example 3 toothpaste) A toothpaste was prepared with the following composition.

Dibasic calcium phosphate 45.0 zero glycerin
20.0' Carboxymethyl cellulose 1.0 Sodium lauryl sulfate 1.8 Sacchulin 0.1 Ethyl paraoxybenzoate 0.1 Shu extract (Example 1) 5.0 Black tea powder
2.0 Flavor 1.5 Silk Ice 23.5 (Example 4 Junsei Dentifrice) A toothpaste was manufactured with the following composition.

Secondary calcium phosphate (dihydrate) Secondary calcium phosphate (anhydrous salt) Saccharin flavor Propylene glycol Sodium lauryl sulfate Carrageenan Purification Water camellia extract (Example 1) Betel extract (Example 1) Total amount 100.0 k (Example 1) Example 5 Mouthwash) A mouthwash was manufactured with the following composition.

Ethyl alcohol 20.0 *Sorvit (aO96 liquid) 15.0 Polyoxyethylene Hydrogenated castor oil 0.5 Ajitsu extract (example)
3.0 Green Tea Extract (Example 1) Saccharin 0.1 Flavor 1.0 0 Ruhexidine Dicurconate 0.005
Ice making 57.395
Total amount 100.09
6 The toothpaste of Example 3, the moist toothpaste of Example 4, and the mouthwash of Example 5 were administered to 20 people with mild periodontal disease at 1 dose each.
After using the test three times a day for 30 days and conducting an interview survey, it was found that 1 out of 20 people found that toothpaste and Junsei toothpaste
An improvement trend was seen in 8 people, and in 13 out of 20 people who used mouthwash.

[Effects of the Invention] As detailed above, the present invention provides an extract and/or a powder of betel nut, black tea, green tea, camellia, and betel nut, which has an inhibitory effect on the inflammatory enzyme hyaluronidase. The present invention provides a toothpaste or mouthwash that is characterized by containing two or more of the above, and provides a toothpaste or mouthwash that is useful for the prevention and treatment of periodontitis, and its effectiveness. It is a big thing.

Procedural Amendment September 27, 1988 1, Indication of Case 1986 Patent Application No. 2゜53': 2 Title (Name) ノ [1: Large/1 Shigo Red No. 1 No. 1, No. 1jζ Hari ))μ′)
) Shigesu Building 330, 2-6-2 Marunouchi, Chiyoda-ku, Tokyo Target of amendment The following matters in the specification of this application will be amended.

Record 1, page 9, line 8 It says "extracts and additions" Correct to "Add extract."

2. On page 13, line 15 It says "toothpaste" I corrected it to ``Junsei Toothpaste.''

3. In the 6th line of page 14, the phrase "Ajitsu extract (Example)" is corrected to "Ajitsu extract (Example 1)."

4, page 14, line 7 "Green tea extract (Example 1) J" "Green tea extract (Example 1) 3. Corrected as OJ.

Claims (1)

[Claims] 1. A toothpaste or mouthwash characterized by containing extracts and/or powders of one or more plants selected from Japanese radish, black tea, green tea, camellia, and betel nut.