JPH03255031A - Periodontosis preventing composition - Google Patents

Abstract

PURPOSE:To obtain a periodontosis preventing composition having extremely additional and synergistic effects on various kinds of periodontosis, comprising a thyme extract, a purified oil and/or oleo-resin. CONSTITUTION:A periodontosis preventing composition comprising (A) a thyme extract, (B) one or more of purified oils, preferably rosemary oil, cinnamon leaf oil, bay oil, an oil of Commiphora abyssinica Engler or cardamon oil and oleo-resins, preferably nutmeg oleo-resin, sage oleo-resin and hop oleo-resin in a ratio of 0.001-2wt.% component A and 0.0005-2wt.% component B, preferably 0.01-0.2wt.% and 0.005-0.2wt.% component B.

Description

[Detailed Description of the Invention] <Industrial Application Field> The present invention is directed to the use of thyme extract and one or more essential oils/oleoresins selected from the group of essential oils and oleoresins, which are natural flavor materials. More specifically, the combination of the thyme extract, essential oil, and oleoresin can be used to prevent periodontal disease, which has significant antibacterial additive, synergistic, and effective effects against various periodontal disease bacteria. The present invention relates to an oral cavity composition that is a periodontal disease preventive composition that is safe and has excellent antibacterial effects and can be widely applied to foods.

<Background of the invention and prior art> The prevalence of periodontal diseases such as gingivitis and pyorrhea was determined by the Ministry of Health and Welfare in 1932.
According to a 2020 survey, 64% of the population suffers from tooth loss, and after the age of 20, the number of people who lose their teeth increases, and after the age of 40, the number of people who lose their teeth increases, and by the age of 70, they have lost 20 teeth. from now on,
As the population continues to age, prevention of periodontal disease is an urgent issue. Furthermore, periodontal disease is also a cause of unpleasant bad breath, and it also poses a modern problem from the perspective of preventing bad breath.

Gingivitis 7 The main causes of periodontal disease represented by pyorrhea are:
It is an anaerobic bacteria found in plaque and tartar in periodontal pockets, and is thought to be a mixed infection.

In healthy periodontal pockets, Durham-positive bacteria usually make up the majority, but as periodontal disease progresses, Durham-negative bacteria increase in number.

In the early stage of gingivitis, Gram-positive bacteria such as Actinomyces viscosus and Actinomyces naeslundii form a flora in the plaque and gingivitis area. As the symptoms progress further and bleeding symptoms occur locally, Durham-negative obligate anaerobes such as Bacteroides gingivalis, Bacteroides melaninogenicus, and Fusibacterium nucleatum proliferate and form the flora.

In general, rapidly progressive periodontitis involves Bacteroides gingivalis, Hubbacterium nucleatum, and adult periodontitis involves Actipacillus actinomycetemcomitans, Capnocytophaga subtigena, etc. It is thought that the causative bacteria differ depending on the disease type, such as the involvement of

In some of these pathogenic bacteria, the endotoxins present on the cell surface activate lymphocytes and macrophages, promote the secretion of collagenase, and increase the production of prostaglandin, which has a bone-resorbing effect, leading to the loss of tissue collagen. , which causes alveolar bone resorption, leading to periodontal tissue collapse and tooth loss.

This series of symptoms progresses chronically and is considered to be one of the diseases that is difficult to cure.

Conventionally, drugs that have anti-inflammatory, anti-allergic, bactericidal, astringent, and cell activating effects have been used as preventive and therapeutic agents for periodontal disease, but these drugs are not sufficiently effective. It's hard to say. In particular, antibiotics are mentioned as drugs with bactericidal action, and the novel antibacterial substance PKI (JP-A-61-109728) prepared from the bacterial cells of Streptococcus faecium and the cultivation of Bacillus sp. TS-107 strain Filtrate (Unexamined Japanese Patent Publication) -17
2326), culture filtrate of Bacillus subis TS-114 strain (Japanese Unexamined Patent Publication No. 1999-72327), and the like. In addition, methylbiperazine derivatives (JP-A-64-83018), pyrrolidine derivatives (JP-A-1I00119), piperidine derivatives and morpholine derivatives (JP-A-1999-00121), etc. have been disclosed as new chemically synthesized fungicides. has been done.

<Problems to be Solved by the Invention> However, the use of antibiotics causes the appearance of resistant bacteria and disturbance of intestinal flora, and there are concerns about the impact on intestinal bacteria. In addition, new chemically synthesized products have safety issues, making it difficult to apply them to foods. There is a need for natural compositions with anti-periodontal bacteria activity that are safer and can be consumed.

Therefore, the present invention aims to solve the above-mentioned problems, and has an excellent antibacterial action and effect.
It is a composition that has a safer ability to prevent dental diseases than conventional antibiotics, and can be used not only in oral hygiene products such as toothpaste, mouthwash, and troches, but also in other products such as chewing gum, candy, etc. The object of the present invention is to provide a natural composition that can also be applied to foods.

As a result of research, the inventors of this application discovered that thyme extract, which is disclosed as a deodorant in a known invention (Japanese Unexamined Patent Publication No. 63-281658), is a deodorant of methyl mercaptan, which is an indicator of bad breath. In addition to its activity, it was discovered that the thyme extract has antibacterial activity against periodontal disease, which was previously unanticipated. However, the antibacterial activity of the thyme extract against periodontitis is weak when used alone, so research has been carried out to further strengthen the antibacterial activity. 1 type or 2 or more types,
We have intensively investigated the additive and synergistic antibacterial effects against periodontal bacteria when used in combination with the above-mentioned thyme extract. the result,
By using both of these in combination, we succeeded in obtaining a composition with strong anti-periodontal bacteria activity, leading to the completion of this invention.

Technical Means for Solving the Problems The present invention solves the problems mentioned above and achieves the objects of the invention described above. ■ Thyme extract, essential oil and one or more of the oleoresins A periodontal disease preventive composition characterized by containing two or more types.

■ The essential oil and oleoresin may be rosemary oil,
cinnamon leaf oil, hay oil, mill oil, cardamom oil,
The periodontal disease preventive composition according to claim 1, which is nutmeg oleoresin, sage oleoresin, or pop oleoresin.

It was decided to consist of the following.

The thyme extract in this invention can be obtained by a known method. That is, Japanese Patent Publication No. 63-2816
As disclosed in No. 58, specifically, thyme (Lamiaceae,
Japanese name: Tymus vu1 Scientific name: Tymus vu1
garis + L, + Tymus service
llum) whole plant or leaves and stems are extracted with a paraffinic solvent, the residue is extracted with an alkyl ether, the solvent is distilled off, and the residue after the solvent is distilled off is further extracted with a ketone solvent,
A green powder obtained by purifying the active ingredient obtained after solvent distillation using silica gel column chromatography,
Melting point 187-200°C1 Ultraviolet absorption spectrum 228
It shows maximum absorption at 293 nm (EtOHsol), and the red light absorption spectrum suggests the presence of hydroxyl groups (O++ groups) and carbonyl groups (>Co groups), and the optical rotation (α] is −137±5. It can be obtained as an active ingredient having the following properties.

The essential oils that can be used in this invention are aroma oils that are often used as fragrance materials and are mainly obtained by steam distillation from plants, and among them, rosemary oil, cinnamon leaf oil, hay oil, mill oil, and cardamom oil. is particularly suitable.

In addition, oleoresin that can be used in this invention,
It is often used as a flavoring material, and is an extract obtained mainly by solvent extraction from plants and distillation of the solvent.In addition to aroma oil, it contains a non-volatile flavor component, a pigment, etc. Of the oleoresins that can be used in this invention, nutmeg oleoresin sage oleoresin 1 hop oleoresin is particularly suitable.

By the way, the Japanese name for the rosemary oil is ``Mannenro''.
, Scientific name: Rosmarims official
It belongs to the Lamiaceae family and is obtained by steam distillation of its leaves.

The scientific name of the ginnamon leaf oil is rC4nnamom.
It is represented by uszeylanicum J, belongs to the Lauraceae family, and is obtained by steam distilling its leaves and twigs.

The mill oil has the scientific name Comm1phora ab.
The hay oil, represented by Pimenta yssinica Engler J, belongs to the family Cilanaceae and is obtained by steam distillation of the resin exuded from its bark, and has the scientific name Pimenta.
It is represented by racemosa J, belongs to the camphor tree, and is obtained by steam distilling its leaves.

The above-mentioned cardamom oil has a Japanese name “Shozuku” and a scientific name “rE”.
lettaria cardamomum Mat
It belongs to the Zingiberaceae family and is obtained by steam distilling the length of the plant, which is plucked after ripening and before splitting.

The nutmeg oleoresin has the scientific name [Myristi
ca fragrance Houffuyn
It belongs to the family Chrysanthemum and is obtained by extracting the seeds in the shell of the fruit with a solvent and distilling off the solvent.

The sage oleoresin has the scientific name 'Salνere'.
It belongs to the Lamiaceae family, and is represented by 'Hicinalis L.', and the pop oleoresin obtained by solvent extraction and solvent distillation from its leaves has the scientific name 'tlumul.
It is represented by usIupulus L and J, belongs to the mulberry family, and is obtained by solvent extraction from the female panicles and distillation of the solvent.

A composition for preventing periodontal disease according to the present invention, that is, a thyme extract and one of the essential oils and oleoresins that are natural flavoring materials.
In order to use a composition containing one or more species as a composition for preventing periodontal disease, the usage form thereof is a liquid formulation,
It may be either a solid agent or a semi-solid agent. Especially,
Preferred formulations include chewing gum, candy, lozenges, mouthwashes, dentifrices, and the like. In addition, the periodontal disease preventive composition according to the present invention can be used not only alone, but also in combination with known antibacterial agents and preservatives in oral compositions, drugs, cosmetics, etc. It can also be applied to other mucous membranes.

0 The amount of the composition according to the present invention to be added to the final product varies depending on the form of the composition, the type of product to be mixed with, the application method and number of applications, and may vary widely depending on the severity of the symptoms. In principle, an effective amount may be added without any particular limitation. For example, when blending into oral hygiene products or foods as a periodontal disease prevention composition, the general amount of thyme extract in the final product is 0.001% to 2% by weight.
% by weight (hereinafter referred to as ``ft%''), preferably 0.01 wt% to 0.2 L%, and the amount of a mixture of one or more essential oils and oleoresins to 0.01 wt% to 0.2 L%. 0005
wt% to 2 wt%, preferably 0.005 wt% to 0
．． 2 Fort L% is suitable.

Furthermore, it is particularly preferable that the composition according to the present invention be blended in the form of a fragrance suitable for the blended product.

<Effect> The periodontal disease preventive composition according to the present invention has antibacterial effects on periodontal disease by composing a composition containing thyme extract, essential oil, and one or more of the oleoresin group. The effects are additive and synergistic, resulting in extremely significant anti-periodontal bacteria activity.

<Example> The periodontal disease preventive composition of the present invention showed excellent results in an antibacterial test against periodontal bacteria.

Below, examples of actual test results will be explained in detail.

) Example of antibacterial test.

(a) Test sample: Thyme extract, various essential oils, and oleoresin were each dissolved in 95% ethanol at a concentration of 5 wt%, and used as test samples.

The thyme extract prepared by the above-mentioned known method was used.

As test essential oils, rosemary oil, cinnamon leaf oil 1 bay oil, mill oil, and cardamom oil were used.

As the test oleoresins, jujube oleoresin, sage oleoresin, and hosippu oleoresin were used.

(b) Test bacterial strains: Seven bacterial strains described in the following (■) to (■) were used as test bacterial strains. In addition, in the text and tables below, each strain is indicated by an abbreviation as necessary.

No, Abbreviation Test strain name ■B, g,; Bacteroides gin
Bacteroides inter
medius ATCC-33563 Bacteroides intermezuus) ■B, m,; Bacteroi
des MeIan: nogenjcus ATCC
5845 (Bacteroides melaninogenicus) ■F, n,;
Fusobacterium nucleatum
ATCC-25586 (Fucibacterium nucleatum) ■To, a,; Actinobacillus
actinomycetemcomitans ATCC
-33384 (Actinomycetemcomitans) ■C, s,; Capnocytophaga sp
Actino
myces viscosus ATCC-1
5987 (Actinomyces viscosus) (C) Test method In the individual tests of various essential oils and oleoresin, the final concentration of each was set to 0.02 wt%, and in the combined test of thyme extract and essential oil/oleoresin. is thyme extract 0.05wt%, various essential oils/oleoresin 0.02wt
CAM broth "Ninosui" liquid medium (1%)
15"C (15 min)) and precultured under carbon dioxide gas aeration (37°C/24 hr).
．． The cells were inoculated at 5% and cultured anaerobically at 37°C for 24 hours.

Among the test bacterial strains, (1) A, v, (Actinomyces viscosus/Actinomyces 34 vtscosus ATC (nee 15987)) were similarly tested using plain heart infusion liquid medium.

The liquid medium contains approximately 3 wt% ethanol, which is a solvent for dissolving the added thyme extract essential oil and oleoresin.
Although present at a high concentration, no effect on the growth of each of the test bacterial strains was observed.

After culturing, the degree of growth of each plot was measured by absorbance at 600 nm,
The control (Tie 1, the growth rate of each plot when cultured in a medium without the addition of extract, essential oil, or oleoresin) was expressed as 100. Tables 1 and 2 both show the results of this antibacterial test example. Table 1 shows examples of the results of antibacterial activity against each test bacterial strain in individual combinations of various essential oils and various oleoresins. Also,
Table 2 shows examples of antibacterial activity results against each test bacterial strain in combination formulations of thyme extract and various essential oils or various oleoresins.

Furthermore, among the seven types of test bacterial strains, test bacterial strains B, g,
(Bacteroides gingivalis), 8, a, (Actinohacillus actinomycetemcomitans), A,
In a combined test when 0.05 wt% of thyme extract and 0 and 1 wt% of various essential oils and oleoresin were added to three strains of (Actinomyces viscosus), minimal growth inhibition of various essential oils and oleoresin was observed. Concentration (MIC=formerly nimum Inhibitory Concent
ration) was calculated. The results are shown in Table 3.

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Table 1 essential oil Example of test results for oleoresin alone.

(Unit: %) Table 2: Examples of combined test results of essential oils, oleoresin, and thyme extract.

[Additional concentration of thyme extract: 0.05wt%] (Unit: %)
) Note) However, when evaluating the results, if it was less than 1% compared to the control (no additives), it was considered 0%.

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7 8 Table 3: Test strain CB, g Examples of test results.

Minimum inhibitory concentration (MI
C) (Unit: wt%) [Thyme extract concentration: 0.05 wt% and 0 wt%
MICI during concomitant use Note) MTC: Minimum inhibitory concentration was recognized as MIC when it was less than 1% compared to the control.

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From Tables 1 and 2, nutmeg oleoresin showed a broad antibacterial spectrum even when used alone, but when used in combination with thyme extract, it showed an additive and synergistic effect on antibacterial properties.
The growth of all strains was significantly inhibited.

Rosemary oil alone does not have as strong an antibacterial property as nutmeg oleoresin, but when used in combination with thyme extract, it exhibited an additive and synergistic effect on antibacterial properties, significantly inhibiting the growth of all seven tested bacterial strains.

Sage oleoresin, bay oil 2 Cinnamon leaf oil alone does not have as strong antibacterial properties as nutmeg oleoresin, but when used in combination with thyme extract, it is effective against other strains except A, V, and (Actinomyces viscosus) strains. It showed remarkable additive and synergistic effects on antibacterial properties against the six tested bacterial strains.

Hop oleoresin and cardamom oil alone do not have as strong antibacterial properties as jujube oleoresin, but when used in combination with thyme extract, they are effective against A, v, (Actinomyces viscosus) and A, a, (Actipasillus actinomycetem). It showed remarkable additive and synergistic effects in terms of antibacterial properties against the other 5 tested strains, excluding 2 strains of A. comitans).

Mill oil alone shows the broadest antibacterial spectrum next to nutmeg oleoresin, and when combined with thyme extract, B, m,
(Hachte 1-1 Ides melaninogenicus) and F
, m, (Fucibacterium nucleatum) showed a remarkable additive synergistic effect on the other five tested bacterial strains except for two strains.

ii) Prescription example using the periodontal disease preventive composition according to the present invention.

Next, examples of combinations of combination compositions of the thyme extract of the present invention and fragrances containing one or more of essential oils and oleoresins, which are natural fragrance materials, will be shown, but the combinations are limited to the following examples. It is not something that will be done. Incidentally, the compounded fragrances for various products listed below include fragrances in which one or more of the essential oils and oleoresins according to the present invention are appropriately blended, and which are arranged to suit the use of each product. It is.

■ Chewing gum wt%/Gum base---1-,------------35/Calcium carbonate-1------------
−−−2・Powdered sugar−−−−−−−−−−−−
-----1-40 Glucose---1---2
0. Thyme extract --11--0. Fragrance for togum −−−−−−−−−−−−−−−−−−−
---1 (The fragrance contains approximately 10 to 20% of one or more of essential oils and oleoresins)・Water ---
----------1 Remaining amount (total amount is 100) ■Candy, Granulated sugar syrup, Citric acid thyme extract wt% 0 5 0.05 1 2 ・Flavor for candy ----------- ---11---
----0,2 (The fragrance contains about 10-20% of one or more of essential oils and oleoresins) Water evaporation (Total amount: 100) Production method> First, mix granulated sugar and starch syrup. and about 150"C
After heating and boiling down to a moisture concentration of 2 to 3%, about 12
Cool to 0°C, then add the other ingredients, stir well to make it uniform, and then finish the product.

■ Mouthwash, 95% ethanol Polyoxyethylene Hydrogenated castor oil, Glycerin, Sodium saccharin, Sodium benzoate Thyme extract wt% 5 Flavor for mouthwash ---1=----0.05 (in the fragrance) , contains about 10 to 20% of one or more of essential oils and oleoresins), pigment (Blue No. 1 0.1% solution')---0,1, purified water -m---Remaining amount (The total amount is 100) ■ Toothpaste %・Dibasic calcium phosphate dihydrate = 50・Glycerin − −−−−−20 Sour lauryl sulfate・Rium−−−−−−Carrageenan−−− ---1----1 allantoin -0,
Totime Extract・－－１－－－－－－－－－－－－～
0.1 Flavoring for toothpaste---
1 (The fragrance contains approximately 10-20% of one or more of essential oils and oleoresins) - Purified water --- ---
Remaining amount (total amount: 100) <Effect of the invention> The periodontal disease preventive composition according to the present invention constitutes a composition containing a thyme extract and one or more of essential oils and oleoresin group. By doing so, the antibacterial effects against periodontal bacteria are added and synergized, and the anti-periodontal activity effect is much more pronounced than the effects based on thyme extract, essential oil, and oleoresin alone. Moreover, since the constituent raw materials of the composition for preventing periodontal disease according to the present invention are all derived from natural substances, it is extremely safe, and the scope of application can be expanded not only to oral hygiene products but also to foods. It has a remarkable effect of achieving the purpose of the invention.

Claims (2)

[Claims] (1) Thyme extract, essential oil and oleoresin group 1
1. A periodontal disease preventive composition comprising one or more species. (2) Claim 1, wherein the essential oil and oleoresin are rosemary oil, cinnamon leaf oil, bay oil, mill oil, cardamom oil, nutmeg oleoresin, sage oleoresin, or hop oleoresin. Periodontal disease prevention composition.