JPH0421634A - Multiplication suppressant of microorganism of periodontal disease - Google Patents
Multiplication suppressant of microorganism of periodontal diseaseInfo
- Publication number
- JPH0421634A JPH0421634A JP2126331A JP12633190A JPH0421634A JP H0421634 A JPH0421634 A JP H0421634A JP 2126331 A JP2126331 A JP 2126331A JP 12633190 A JP12633190 A JP 12633190A JP H0421634 A JPH0421634 A JP H0421634A
- Authority
- JP
- Japan
- Prior art keywords
- oil
- suppressant
- periodontal disease
- application example
- sandalwood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000028169 periodontal disease Diseases 0.000 title claims abstract description 24
- 244000005700 microbiome Species 0.000 title 1
- 244000037364 Cinnamomum aromaticum Species 0.000 claims abstract description 8
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims abstract description 8
- 240000002505 Pogostemon cablin Species 0.000 claims abstract description 5
- 235000011751 Pogostemon cablin Nutrition 0.000 claims abstract description 5
- 235000008632 Santalum album Nutrition 0.000 claims abstract description 5
- 240000000513 Santalum album Species 0.000 claims abstract 3
- 239000003966 growth inhibitor Substances 0.000 claims description 10
- 239000000341 volatile oil Substances 0.000 claims description 8
- 241000147041 Guaiacum officinale Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 235000017803 cinnamon Nutrition 0.000 claims description 5
- 229940091561 guaiac Drugs 0.000 claims description 5
- 244000223760 Cinnamomum zeylanicum Species 0.000 claims 1
- 235000009508 confectionery Nutrition 0.000 abstract description 19
- 241000894006 Bacteria Species 0.000 abstract description 17
- 235000015218 chewing gum Nutrition 0.000 abstract description 12
- 229940112822 chewing gum Drugs 0.000 abstract description 12
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 10
- 235000013305 food Nutrition 0.000 abstract description 9
- 208000007565 gingivitis Diseases 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- 210000000214 mouth Anatomy 0.000 abstract description 4
- 241000935112 Bulnesia Species 0.000 abstract description 2
- 235000021511 Cinnamomum cassia Nutrition 0.000 abstract description 2
- 241000605072 Cinnamomum verum Species 0.000 abstract 1
- 235000004310 Cinnamomum zeylanicum Nutrition 0.000 abstract 1
- 101100113998 Mus musculus Cnbd2 gene Proteins 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 20
- 239000010671 sandalwood oil Substances 0.000 description 15
- 239000003921 oil Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 11
- 235000000346 sugar Nutrition 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000001738 pogostemon cablin oil Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000723347 Cinnamomum Species 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 235000021552 granulated sugar Nutrition 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241000605862 Porphyromonas gingivalis Species 0.000 description 5
- 241001135221 Prevotella intermedia Species 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- 235000019645 odor Nutrition 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 239000006188 syrup Substances 0.000 description 4
- 235000020357 syrup Nutrition 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 239000001927 guaiacum sanctum l. gum oil Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000001256 steam distillation Methods 0.000 description 3
- 241000606750 Actinobacillus Species 0.000 description 2
- 241000606125 Bacteroides Species 0.000 description 2
- 241000605986 Fusobacterium nucleatum Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000207923 Lamiaceae Species 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 241000221035 Santalaceae Species 0.000 description 2
- 208000008312 Tooth Loss Diseases 0.000 description 2
- FUWUEFKEXZQKKA-UHFFFAOYSA-N beta-thujaplicin Chemical compound CC(C)C=1C=CC=C(O)C(=O)C=1 FUWUEFKEXZQKKA-UHFFFAOYSA-N 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000008601 oleoresin Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229940098465 tincture Drugs 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- QIVUCLWGARAQIO-OLIXTKCUSA-N (3s)-n-[(3s,5s,6r)-6-methyl-2-oxo-1-(2,2,2-trifluoroethyl)-5-(2,3,6-trifluorophenyl)piperidin-3-yl]-2-oxospiro[1h-pyrrolo[2,3-b]pyridine-3,6'-5,7-dihydrocyclopenta[b]pyridine]-3'-carboxamide Chemical compound C1([C@H]2[C@H](N(C(=O)[C@@H](NC(=O)C=3C=C4C[C@]5(CC4=NC=3)C3=CC=CN=C3NC5=O)C2)CC(F)(F)F)C)=C(F)C=CC(F)=C1F QIVUCLWGARAQIO-OLIXTKCUSA-N 0.000 description 1
- FFTVPQUHLQBXQZ-KVUCHLLUSA-N (4s,4as,5ar,12ar)-4,7-bis(dimethylamino)-1,10,11,12a-tetrahydroxy-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide Chemical compound C1C2=C(N(C)C)C=CC(O)=C2C(O)=C2[C@@H]1C[C@H]1[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]1(O)C2=O FFTVPQUHLQBXQZ-KVUCHLLUSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 235000007866 Chamaemelum nobile Nutrition 0.000 description 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000111489 Gardenia augusta Species 0.000 description 1
- 241000331120 Krameria cistoidea Species 0.000 description 1
- 241000218195 Lauraceae Species 0.000 description 1
- 244000042664 Matricaria chamomilla Species 0.000 description 1
- 235000007232 Matricaria chamomilla Nutrition 0.000 description 1
- -1 Patchouli oil Chemical class 0.000 description 1
- 241001135223 Prevotella melaninogenica Species 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 235000008631 Santalum Nutrition 0.000 description 1
- 241001496113 Santalum Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- TUFYVOCKVJOUIR-UHFFFAOYSA-N alpha-Thujaplicin Natural products CC(C)C=1C=CC=CC(=O)C=1O TUFYVOCKVJOUIR-UHFFFAOYSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229960003260 chlorhexidine Drugs 0.000 description 1
- 239000010630 cinnamon oil Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 229960004023 minocycline Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002324 mouth wash Substances 0.000 description 1
- AYOOGWWGECJQPI-NSHDSACASA-N n-[(1s)-1-(5-fluoropyrimidin-2-yl)ethyl]-3-(3-propan-2-yloxy-1h-pyrazol-5-yl)imidazo[4,5-b]pyridin-5-amine Chemical compound N1C(OC(C)C)=CC(N2C3=NC(N[C@@H](C)C=4N=CC(F)=CN=4)=CC=C3N=C2)=N1 AYOOGWWGECJQPI-NSHDSACASA-N 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- XULSCZPZVQIMFM-IPZQJPLYSA-N odevixibat Chemical compound C12=CC(SC)=C(OCC(=O)N[C@@H](C(=O)N[C@@H](CC)C(O)=O)C=3C=CC(O)=CC=3)C=C2S(=O)(=O)NC(CCCC)(CCCC)CN1C1=CC=CC=C1 XULSCZPZVQIMFM-IPZQJPLYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 235000019565 spicy aroma Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- 229930007845 β-thujaplicin Natural products 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Fats And Perfumes (AREA)
- Confectionery (AREA)
- Cosmetics (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は歯周病及び歯肉炎の発症と進行に深く関与して
いるバクテロイデス・ジンジバリス（Ｂａｃｔｅｒｏｉ
ｄｅｓ ｇｉｎｇｉｖａｌｉｓ）、バクテロイデス・イ
ンターメディウス（Ｂ、 ｉｎｔｅｒｍｅｄｉｕｓ）、
バクテロイデス・メラニノジエニカス（Ｂ、 ｍｅｌａ
ｎｉｎｏｇｅｎｉｃｕｓ）、アクチッパシラス・アクチ
ノマイセテムコミタンス（Ａｃｔｉｎｏｂａｃｉｌｌｕ
ｓ ａｃｔｉｎｏｍｙｃｅｔｅｍｃｏｍｉｔａｎｓ）、
フシバクテリウム・ヌクレアタム（Ｆｕｓｏｂａｃｔｅ
ｒｉｕｍ ｎｕｃｌｅａｔｕｍ）等に代表される各種口
腔内細菌に対して優れた抗菌作用を示す、歯周病菌増殖
抑制剤に関するものである。Detailed Description of the Invention (Field of Industrial Application) The present invention is directed to the treatment of Bacteroides gingivalis, which is deeply involved in the onset and progression of periodontal disease and gingivitis.
B. gingivalis), B. intermedius (B. intermedius),
Bacteroides melaninogienicus (B, mela)
ninogenicus), Actipacillus actinomycetemcomitans (Actinobacillus)
s actinomycetemcomitans),
Fusibacterium nucleatum
The present invention relates to a periodontal disease bacterial growth inhibitor that exhibits excellent antibacterial activity against various oral bacteria such as P. rium nucleatum.
（従来の技術）
歯周病は歯肉炎か進行して起こるものであり、歯を失う
原因の５０９４５か歯周病である。２０代後半から歯周
病の罹患率は高くなり、中高年にかけては、約５０％の
人か（歯肉炎をいれると８０％）罹患している。−船釣
に歯周病の発症と進行に歯肉溝内プラーク中のプラム陰
性嫌気性桿菌か深・（かかわっているとされている。と
りわけバクテロイデス・ジンジバリス（Ｂ、 ｇｉＢｉ
νａｌｉｓ）、バクテロイデス・インターメディウス（
Ｂ、 ｉｎｔｅｒｍｅｄｉｕｓ）、 バクテロイデス・
メラニノジエニカス（Ｂ、 ｍｅｌａｎｉｎ。(Prior Art) Periodontal disease is caused by the progression of gingivitis, and is the cause of tooth loss. The prevalence of periodontal disease increases from the late twenties onwards, and in middle-aged and older adults, approximately 50% (80% if gingivitis is included). - Plum-negative anaerobic bacilli in gingival crevicular plaque are believed to be involved in the onset and progression of periodontal disease during boat fishing. In particular, Bacteroides gingivalis (B, giBi)
νalis), Bacteroides intermedius (
B, intermedius), Bacteroides
B. melanin.
ｇｅｎｉｃｕｓ）、アクチッパシラス・アクチノマイセ
テムコミタンス（Ａｃｔｉｎｏｂａｃｉｌｌｕｓ ａｃ
ｔｉｎｏｍｙｃｅｊｅ＋ｎｃ。Genicus), Actinobacillus ac
tinomyceje+nc.
ｍ１ｔａｎｓ）、フシバクテリウム・ヌクレアタム（Ｆ
ｕｓ。m1tans), Fucibacterium nucleatum (F
us.
ｂａｃｔｅｒｉｕｍ ｎｕｃｌｅａｔｕｍ）等か、種々
のタイプの歯周病の発症、進行に関連している可能性か
示唆されている（中高光−ら 歯周病の抗生物質治療
臼歯周誌、２９巻２号４６３−４７１．１９８７）。It has been suggested that bacteria such as Bacterium nucleatum may be related to the onset and progression of various types of periodontal disease (Hikaru Nakataka et al. Antibiotic Treatment of Periodontal Disease)
Journal of Molar Periodontics, Vol. 29, No. 2, 463-471.1987).
それ故、歯周病の発症と進行にかかわる細菌を制御する
ことは歯周病の治屓において有効な手段てある。歯周病
、歯肉炎の発症に大きく関与している各種口腔内細菌の
生育を阻害する薬剤としてはクロルヘキシジン、トラネ
キサム酸や、テトラサイクリン、ミノサイクリン等の抗
生物質の使用が知られている外、天然物としてはカミツ
レチンキ。Therefore, controlling bacteria involved in the onset and progression of periodontal disease is an effective means for curing periodontal disease. Antibiotics such as chlorhexidine, tranexamic acid, tetracycline, and minocycline are known to be used as drugs that inhibit the growth of various oral bacteria that are significantly involved in the onset of periodontal disease and gingivitis, as well as natural products. Chamomile tincture.
ラタニアチンキ、ヒノキチオール等が利用されている。Ratania tincture, hinokitiol, etc. are used.
（発明か解決しようとする課題）
しかしなから、これら公知の口腔内細菌の生育阻害剤は
口の中で使用する場合には安全性に問題があったり、投
与した際不快臭かする等の欠点かあった。(Problem to be solved by the invention) However, these known oral bacteria growth inhibitors may have safety problems when used in the mouth, or may emit an unpleasant odor when administered. There were some drawbacks.
本発明の目的は食品として身近に利用されているものか
ら、歯周病菌に対し抗菌性をもち、しかも不快臭のない
ものを選択し、それより成る歯周病菌増殖抑制剤を提供
することにある。The purpose of the present invention is to select from those commonly used as foods that have antibacterial properties against periodontal disease bacteria and have no unpleasant odor, and to provide a periodontal disease bacterial growth inhibitor made of them. be.
（課題を解決するための手段） 本発明は、ビヤクダン、カッシャ、シンナモン。(Means for solving problems) The present invention uses sandalwood, cassia, and cinnamon.
グアヤツク、パチュリから選択される精油成分から成る
歯周病菌の増殖抑制剤である。This is a growth inhibitor for periodontal disease bacteria that consists of essential oil components selected from guaiac and patchouli.
本発明で用いられるビヤクダンオイルは公知の化合物で
あり、例えばビヤクダン科Ｓａｎｔａｌｕｍ ａｌｂｕ
ｍ Ｌ、の根及び心材を水蒸気蒸留することによって得
られる、やや粘ちょうな黄色の液体で、ソフトな甘いウ
ツデイ調の香気を持つ。The sandalwood oil used in the present invention is a known compound, for example Santalum albu
It is a slightly viscous yellow liquid obtained by steam distillation of the roots and heartwood of M.L., and has a soft, sweet, mushy aroma.
本発明で用いられるカッシャオイルは公知の化合物であ
り、例えばクスノキ科Ｃ１口ｎａｍｏｍｕｍ ｃａｓｓ
ｉａの樹皮乾燥物を水蒸気蒸留して得られる黄色〜褐色
の油状液体であり、甘くスパイシーな香りを持ち、コー
ラ等の食品のフレーバーとして一般に用いられている。The cassia oil used in the present invention is a known compound, for example, from Lauraceae C1 namomum cassia
It is a yellow to brown oily liquid obtained by steam distilling the dried bark of A. ia. It has a sweet and spicy aroma and is commonly used as a flavor for foods such as cola.
本発明で用いられるシンナモンの精油は公知の化合物で
あり、例えばクスノキ科Ｃｉｎｎａｍｏｍｕｍ ｚｅｙ
ｌａｎｉｃｕｍ等の樹皮乾燥物を有機溶媒（アルコール
等）で抽出した後、減圧濃縮して得られる（シンナモン
オレオレジン）。せ美な芳香と刺激味を持ち、コーラ系
飲料、チューインガム、キャンデイ−等の調味料として
用いられている。The essential oil of cinnamon used in the present invention is a known compound, for example, Cinnamomum zey
Cinnamon oleoresin is obtained by extracting the dried bark of C. lanicum etc. with an organic solvent (alcohol etc.) and then concentrating it under reduced pressure. It has a beautiful aroma and pungent taste, and is used as a seasoning for cola drinks, chewing gum, candy, etc.
本発明で用いられるグアヤツクオイルは公知の化合物で
あり、例えば南米で生産されるハマビシ科Ｂｕｌｎｅｓ
ｉａ ｓｕｒｍｉｅｎｔｉ Ｌｏｒ、の心材を乾燥し、
オガクズ状にしたものを水蒸気蒸留して得られる（グア
ヤックウソトオイル）。室温では黄白色の固塊状物質で
あるか、４０〜５０°Ｃに加温することによって粘稠な
黄色の液体となる。甘いローズ様の香気を有し、フレー
バーとして用いられている。The guaiac oil used in the present invention is a known compound, for example, guaiac oil produced in South America.
The heartwood of ia surmienti Lor is dried,
Obtained by steam distillation of sawdust (guaiac oil). At room temperature, it is a yellow-white solid mass, or when heated to 40-50°C, it becomes a viscous yellow liquid. It has a sweet rose-like aroma and is used as a flavor.
本発明で用いられるパチュリオイルは公知の化合物であ
り、例えばシソ科の多年生草木であるＰａｔｃｈｏｕｌ
ｙ （パチュリ）の葉の乾燥物を粉砕し、水蒸気蒸留し
て得られる精油であり、コーラタイプの飲料のフレーバ
ーとして一般に用いられている。Patchouli oil used in the present invention is a known compound, such as Patchouli oil, a perennial plant of the Lamiaceae family.
It is an essential oil obtained by crushing dried leaves of Patchouli and steam distilling it, and is commonly used as a flavor for cola-type drinks.
本発明の歯周病菌の増殖抑制剤の投与形態としては口腔
中で比較的滞留時間の長いチューインガム、キャンデイ
−等の食品に配合させることによりその効果か期待てき
る。その配合量は食品の形態２種類等によって必ずしも
一様ではないかその食品中０．００３〜５重量％、好ま
しくは０．１−１重量％配合するのか一般的である。ま
たそれらは単独で用いても混合で用いてもよい。As for the dosage form of the growth inhibitor of periodontal disease bacteria of the present invention, its effects can be expected by incorporating it into foods such as chewing gum and candy, which have a relatively long residence time in the oral cavity. The amount of the compound may not necessarily be the same depending on the type of food, but it is generally 0.003 to 5% by weight, preferably 0.1 to 1% by weight of the food. Moreover, they may be used alone or in combination.
また食品に限らず、歯磨、洗口液等にも適用か可能であ
り、歯周病の治療への応用か期待てきる。Furthermore, it can be applied not only to foods but also to toothpastes, mouth rinses, etc., and is expected to be applied to the treatment of periodontal disease.
以下に実施例を挙げて、本発明を更に詳細に説明する。The present invention will be explained in more detail with reference to Examples below.
（実施例）
実施例に先立って歯周病菌に対する抗菌活性の試験方法
について説明する。(Example) Prior to the Examples, a method for testing antibacterial activity against periodontal disease bacteria will be explained.
抗菌活性試験方法
２倍段階希釈により０．０２〜４ ｍ ｇ／ｍ ｐに調
製した本発明の歯周病菌増殖抑制剤（以下「試料Ｊとす
る）各々を含む変法ＣＡＭ寒天培地（日永製薬製）に、
予めＣＡＭブイヨン培地（日永製薬製）にて３７°Ｃ１
２４時間嫌気培養した被検菌を１白金耳接種し、３７°
Ｃて５日間嫌気培養した後、菌の生育の有無を肉眼で判
定した。Antibacterial activity test method Modified CAM agar medium (Hinaga) containing each of the periodontal disease bacterial growth inhibitors of the present invention (hereinafter referred to as "sample J") prepared to 0.02 to 4 mg/mp by two-fold serial dilution. Pharmaceutical products),
Preliminary temperature at 37°C in CAM broth medium (manufactured by Hinaga Pharmaceutical Co., Ltd.)
One platinum loop of test bacteria cultured anaerobically for 24 hours was inoculated, and the plate was incubated at 37°
After culturing anaerobically for 5 days at C, the presence or absence of bacterial growth was visually determined.
完全に生育か阻止されている平板に含まれている試料の
最低濃度をもって、最小生育阻止濃度（ｍ ｇ ／ ｍ
ｌ ）とした。The minimum concentration of the sample contained in the plate where growth is completely inhibited is the minimum growth inhibitory concentration (mg/m
l).
尚、被検菌としては、バクテロイデス・ジンジバリス（
Ｂａｃｔｅｒｏｉｄｅｓ ｇｉｎｇｉｖａｌｉｓ）Ａ
Ｔ ＣＣ３３２７７株、バクテロイデス・インターメデ
ィウス（Ｂ、 ｉｎｔｅｒｍｅｄｉｕｓ） Ａ Ｔ Ｃ
Ｃ１５０３２株、バクテロイデス・メラニノジエニカス
（Ｂ、 ｍｅ ｔａｎ ｉｎｏ−ｇｅｎｉｃｕｓ）Ａ
Ｔ ＣＣ２５８４５株、アクチッパシラス・アクチノマ
イセテムコミタンス（Ａｃｔｉｎｏｂａｃｉｆｌｕｓ
ａｃｔｉｎｏｍｙｃｅｔｅｍｃｏｍｉｔａｎｓ） Ａ
Ｔ ＣＣ２９５２２株、フシバクテリウム・ヌクレアタ
ム（Ｆｕｓｏｂａｃｔｅｒｉｕｍ ｎｕｃｌｅａｔｕｍ
） Ａ Ｔ ＣＣ１０９５３株を使用した。The test bacterium is Bacteroides gingivalis (
Bacteroides gingivalis)A
T CC33277 strain, Bacteroides intermedius (B, intermedius) A T C
C15032 strain, Bacteroides melaninogenicus (B, metan ino-genicus) A
T CC25845 strain, Actipacillus actinomycetemcomitans (Actinobaciflus
actinomycetemcomitans) A
T CC29522 strain, Fusobacterium nucleatum
) A T CC10953 strain was used.
実施例１ ビヤクダンオイル
ビヤクダンの材部及び根部の細断物２００ｇをＩｆの水
と共に約５０時間水蒸気蒸留し、精油を約１０ｇ得た。Example 1 Sandalwood oil 200 g of shredded sandalwood wood and roots were steam-distilled with If water for about 50 hours to obtain about 10 g of essential oil.
これを試料として、前述の方法にて抗菌活性試験を行い
、結果を第１表に示した。Using this as a sample, an antibacterial activity test was conducted using the method described above, and the results are shown in Table 1.
実施例２ カッシャオイル
クスノキ科Ｃｉｎｎａｍｏｍｕｍ ｃａｓｓｉａの樹皮
乾燥物２００ｇを粉砕し、ｉＩ！の水と共に約８時間水
蒸気蒸留し、精油を約２ｇ得た。Example 2 200 g of dried bark of Cassia oil, Cinnamomum cassia, was crushed and iI! About 2 g of essential oil was obtained by steam distillation with water for about 8 hours.
これを試料として、前述の方法にて抗菌活性試験を行い
、結果を第１表に示した。Using this as a sample, an antibacterial activity test was conducted using the method described above, and the results are shown in Table 1.
実施例３ シンナモンオレオレンン
クスノキ１４ Ｃｉｎｎａｍｏｍｕｍ ｚｅｙｌａｎｉ
ｃｕｍの樹皮乾燥物２００ｇを粉砕し、１１の水／エタ
ノール（Ｉ／１）溶剤で、室温にて１週間抽出し、抽出
液を濾過器にかけ濾液を減圧濃縮し、ペースト状の才し
オレンジを約３ｇ得た。Example 3 Cinnamomum zeylani 14
cum 200g of dried bark was crushed and extracted with 11 water/ethanol (I/1) solvent at room temperature for one week.The extract was filtered and the filtrate was concentrated under reduced pressure to obtain a paste-like old orange. About 3g was obtained.
これを試料として、前述の方法にて抗菌活性試験を行い
、結果を第１表に示した。Using this as a sample, an antibacterial activity test was conducted using the method described above, and the results are shown in Table 1.
実施例４ グアヤックウッドオイル
ハマビシ科Ｂｕｌｎｅｓｉａ ｓｕｒｍｉｅｎｔｉ Ｌ
ｏｒ、の心材を乾燥したちの２００ｇを粉砕し、１ｊ２
の水と共に約５０時間水蒸気蒸留し、精油を約８ｇ得た
。Example 4 Guayac wood oil Bulnesia surmienti L
Crush 200g of dried heartwood of or, 1j2
It was steam-distilled with water for about 50 hours to obtain about 8 g of essential oil.
これを試料として、前述の方法にて抗菌活性試験を行い
、結果を第１表に示した。Using this as a sample, an antibacterial activity test was conducted using the method described above, and the results are shown in Table 1.
実施例５ パチュリオイル
シソ科パチュリの葉の乾燥物２００ｇを細断し、ｌｊ’
の水と共に約８時間水蒸気蒸留し、精油を約４ｇ得た。Example 5 Patchouli oil 200g of dried patchouli leaves of the Lamiaceae family were shredded and lj'
It was steam-distilled with water for about 8 hours to obtain about 4 g of essential oil.
これを試料として、前述の方法にて抗菌活性試第１表の
結果から各種香料は菌の増殖抑制に優れた効果を示すこ
とは明らかである。Using this as a sample, antibacterial activity was tested using the method described above. From the results shown in Table 1, it is clear that various fragrances exhibit excellent effects in inhibiting the growth of bacteria.
以下に、本発明の歯周病菌の増殖抑制組成物の食品への
応用例を示す。Examples of the application of the composition for inhibiting the growth of periodontal disease bacteria of the present invention to foods are shown below.
応用例−１（チューインガムの製造）
チューインガムベース
粉糖
ブドウ糖
水飴
２０．０
５１．９
１０．０
１８．０
製法
４０℃に保温した全量のチューインガムベース及び全量
の水飴をニーダ−に投入して１０分間混練し粉糖の１／
３量及び全量のブドウ糖を投入して５分間、次いて粉糖
の１／３量を投入して５分間混練した。次に、増殖抑制
剤であるビヤクダンオイルを残りの１／３量の粉糖に混
合してから投入し５分間混練してガムミックスを得た。Application example-1 (Manufacturing of chewing gum) Chewing gum base Powder sugar glucose starch syrup 20.0 51.9 10.0 18.0 Manufacturing method The whole amount of chewing gum base and the whole amount of starch syrup kept at 40°C were put into a kneader and kneaded for 10 minutes. 1/2 of kneaded powdered sugar
Three amounts and the entire amount of glucose were added and kneaded for 5 minutes, then 1/3 amount of powdered sugar was added and kneaded for 5 minutes. Next, sandalwood oil, which is a growth inhibitor, was mixed with the remaining 1/3 amount of powdered sugar, and then added and kneaded for 5 minutes to obtain a gum mix.
応用例−２（チューインガムの製造）
製法
応用例−１のビヤクダンオイルの代わりに力・ノンヤオ
イルを用いた他は応用例−１と同様行ないガムミックス
を得た。Application Example 2 (Manufacture of Chewing Gum) A gum mix was obtained in the same manner as Application Example 1 except that chikara nonya oil was used instead of sandalwood oil.
応用例−３（チューインガムの製造）
製法
応用例＝１のビヤクダンオイルの代わりにシンナモンオ
イルを用いた他は応用例−１と同様行ないガムミックス
を得た。Application Example 3 (Manufacture of Chewing Gum) A gum mix was obtained in the same manner as Application Example 1 except that cinnamon oil was used instead of sandalwood oil in Production Method Application Example 1.
応用例−４（チューインガムの製造）
製法
応用例−１のビヤクダンオイルの代わりにグアヤツクウ
ッドオイル０．２重量％を用い、粉糖を５１．８重量％
にした他は応用例−１と同様行ないガムミックスを得た
。Application example-4 (Manufacturing of chewing gum) Using 0.2% by weight of guaiac wood oil instead of sandalwood oil in manufacturing method application example-1, and 51.8% by weight of powdered sugar.
A gum mix was obtained in the same manner as in Application Example 1, except for the following.
応用例−５（チューインガムの製造）
製法
応用例−１のビヤクダンオイルの代わりにパチュリオイ
ル０．２重量％を用い、粉糖を５１．８重量％にした他
は応用例−１と同様行ないガムミックスを得た。Application example-5 (manufacture of chewing gum) Production method The same procedure as application example-1 was used except that 0.2% by weight of patchouli oil was used instead of sandalwood oil and powdered sugar was changed to 51.8% by weight. Got the gum mix.
応用例−６（チューインガムの製造）
製法
応用例−１のビヤクダンオイルの代わりにビヤクダンオ
イル、カッツヤオイル、ノンナモンオし才しジン、グア
ヤソクウソトオイル、パチュリオイルを各々０．１重量
％混合したちの０．５重量％を用い、粉糖を５１，５重
Ｉ％にした他は応用例−１と同様行ないガムミックスを
得た。Application example - 6 (manufacturing of chewing gum) Instead of sandalwood oil in production method application example - 1, 0.1% by weight each of sandalwood oil, katsuya oil, Nonnamon's gifted gin, guaya sokusoto oil, and patchouli oil are mixed. A gum mix was obtained in the same manner as in Application Example 1, except that 0.5% by weight of Shitachi was used and powdered sugar was changed to 51.5% by weight.
応用例−７（キャンデイ−の製造）
グラニユー糖
水飴（水分３０％）
クエン酸
クチナン色素
６０．３
５６．０
０．３
０．１
製法
予ｉ溶解釜に、グラニュー糖、水飴及び少量の水を投入
し、−度沸騰させてグラニユー糖を完全に溶解し、この
混合物を真空クツカーにポンプて送り込み、真空度４６
０ｍｍＨｇ、温度１３０°Ｃて煮詰めた後、取出釜に取
り混合釜に移した。次いて増殖抑制組成物であるヒヤク
ダンオイル、クエン酸及びクチナソ色素を投入して十分
混合後、冷却盤上に広げキャンデイ−マスを得た。Application example 7 (manufacturing of candy) Granulated sugar starch syrup (30% moisture) Cutinane citrate pigment 60.3 56.0 0.3 0.1 Preparation method In a dissolving pot, granulated sugar, starch syrup, and a small amount of water are added. The granulated sugar is completely dissolved by boiling at -46°C, and the mixture is pumped into a vacuum cleaner at a vacuum level of 46°C.
After boiling down at 0 mmHg and a temperature of 130°C, the mixture was taken into a takeout pot and transferred to a mixing pot. Next, hyakudan oil, citric acid, and gardenia pigment, which are growth-inhibiting compositions, were added, thoroughly mixed, and then spread on a cooling plate to obtain a candy mass.
応用例−８（キャンデイ−の製造）
製法
応用例−７のビヤクダンオイルの代わりにカッツヤオイ
ルを用いた他は応用例−７と同様行ないキャンデイ−マ
スを得た。Application Example 8 (manufacture of candy) Candy mass was obtained in the same manner as Application Example 7, except that katsuya oil was used instead of sandalwood oil.
応用例−９（キャンデイ−の製造）
製法
応用例−７のビヤクダンオイルの代わりにンンナモンオ
しオレジンを用いた他は応用例−７と同様行ないキャン
デイ−マスを得た。Application Example 9 (Manufacture of Candy) Candy mass was obtained in the same manner as Application Example 7 except that nannamon oil resin was used instead of sandalwood oil.
応用例−１Ｏ（キャンデイ−の製造）
製法
応用例−７のビヤクダンオイルの代わりにグアヤツクウ
ッドオイルを０．２重量部を用い、グラニユー糖を６０
．２重量部用いた他は応用例−７と同様行ないキャンデ
イ−マスを得た。Application example - 1O (manufacture of candy) Manufacturing method Application example - 0.2 parts by weight of guaiac wood oil was used instead of sandalwood oil, and 60 parts by weight of granulated sugar was used.
．． A candy mass was obtained in the same manner as in Application Example 7 except that 2 parts by weight was used.
応用例−１１（キャンデイ−の製造）
製法
応用例−７のビヤクダンオイルの代わりにパチュリオイ
ルを０．２重量部用い、グラニユー糖を６０．２重量部
用いた他は応用例−７と同様行ないキャンデイ−マスを
得た。Application example 11 (manufacture of candy) Same as application example 7 except that 0.2 parts by weight of patchouli oil and 60.2 parts by weight of granulated sugar were used instead of sandalwood oil in manufacturing method application example 7. I did so and got some candy trout.
応用例−１２（キャンデイ−の製造）
製法
応用例−７のビヤクダンオイルの代わりにビヤクダンオ
イル、カッシャオイル、シンナモンオレオレジン、グア
ヤツクウッドオイル、パチュリオイルを各々０．１重量
部混合したもの０，５重量部を用い、グラニユー糖を５
９．９重量部用いた他は応用例−７と同様行ないキャン
デイ−マスを得た。Application example 12 (manufacturing of candy) Sandalwood oil, cassia oil, cinnamon oleoresin, guaiac wood oil, and patchouli oil were mixed in an amount of 0.1 part by weight in place of the sandalwood oil in production method application example 7. Using 0.5 parts by weight of sugar, 5 parts by weight of granulated sugar
A candy mass was obtained in the same manner as in Application Example 7 except that 9.9 parts by weight was used.
これらのチューインガム・キャンデイ−には不快臭はな
かった。These chewing gum candies had no unpleasant odor.
（発明の効果）
本発明の歯周病菌の増殖抑制剤は、歯を失う原因となる
歯周病菌の増殖を効果的に阻止する。また従来知られて
いる開園の増殖抑制物質に共通してみうけられる不快臭
か全くなく、食品その他の口腔用組成物に利用するのに
適している。(Effects of the Invention) The growth inhibitor of periodontal disease bacteria of the present invention effectively inhibits the growth of periodontal disease bacteria that cause tooth loss. Furthermore, it has no unpleasant odor commonly found in conventional growth inhibitors, and is suitable for use in foods and other oral compositions.
Claims (1)
ュリから選択される精油成分から成る歯周病菌の増殖抑
制剤。A periodontal disease bacterial growth inhibitor consisting of essential oil components selected from sandalwood, cassia, cinnamon, guaiac, and patchouli.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2126331A JP2582736B2 (en) | 1990-05-15 | 1990-05-15 | Periodontal disease growth inhibitor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2126331A JP2582736B2 (en) | 1990-05-15 | 1990-05-15 | Periodontal disease growth inhibitor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0421634A true JPH0421634A (en) | 1992-01-24 |
| JP2582736B2 JP2582736B2 (en) | 1997-02-19 |
Family
ID=14932543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2126331A Expired - Fee Related JP2582736B2 (en) | 1990-05-15 | 1990-05-15 | Periodontal disease growth inhibitor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2582736B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0429933A (en) * | 1990-05-24 | 1992-01-31 | Lotte Co Ltd | Anti-periodontal disease drug |
| EP1181866A1 (en) * | 2000-08-14 | 2002-02-27 | Givaudan SA | Antibacterial composition comprising Sandela |
| EP1184030A1 (en) * | 2000-08-14 | 2002-03-06 | Givaudan SA | Antibacterial composition comprising trimethylnorbornanylcyclohexanol derivatives |
| KR100611248B1 (en) * | 2004-01-05 | 2006-08-10 | 주식회사 내츄로바이오텍 | Antibacterial and antifungal composition containing plant oil |
| JP2006298824A (en) * | 2005-04-21 | 2006-11-02 | Kao Corp | Inhibitor for skatole production in oral cavity |
| JP2006347947A (en) * | 2005-06-15 | 2006-12-28 | Seiko Awane | Antibacterial agent containing extract of prunus mume flesh |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59175410A (en) * | 1983-03-26 | 1984-10-04 | Kanebo Shokuhin Kk | Cariostatic agent |
| JPH03255031A (en) * | 1990-03-02 | 1991-11-13 | Taiyo Koryo Kk | Periodontosis preventing composition |
-
1990
- 1990-05-15 JP JP2126331A patent/JP2582736B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59175410A (en) * | 1983-03-26 | 1984-10-04 | Kanebo Shokuhin Kk | Cariostatic agent |
| JPH03255031A (en) * | 1990-03-02 | 1991-11-13 | Taiyo Koryo Kk | Periodontosis preventing composition |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0429933A (en) * | 1990-05-24 | 1992-01-31 | Lotte Co Ltd | Anti-periodontal disease drug |
| JPH0645549B2 (en) * | 1990-05-24 | 1994-06-15 | 株式会社ロッテ | Anti periodontal drug |
| EP1181866A1 (en) * | 2000-08-14 | 2002-02-27 | Givaudan SA | Antibacterial composition comprising Sandela |
| EP1184030A1 (en) * | 2000-08-14 | 2002-03-06 | Givaudan SA | Antibacterial composition comprising trimethylnorbornanylcyclohexanol derivatives |
| KR100611248B1 (en) * | 2004-01-05 | 2006-08-10 | 주식회사 내츄로바이오텍 | Antibacterial and antifungal composition containing plant oil |
| JP2006298824A (en) * | 2005-04-21 | 2006-11-02 | Kao Corp | Inhibitor for skatole production in oral cavity |
| JP2006347947A (en) * | 2005-06-15 | 2006-12-28 | Seiko Awane | Antibacterial agent containing extract of prunus mume flesh |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2582736B2 (en) | 1997-02-19 |
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