JP2006298824A - Inhibitor for skatole production in oral cavity - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an inhibitor for skatole production in the oral cavity, safely used in the oral cavity and inhibiting production of skatole by microbes in the oral cavity. <P>SOLUTION: The inhibitor for skatole production in the oral cavity contains one or more species of extracts of plants selected from bay, fennel, sebory, thyme, pimento berry, Rubiaceae, estragon, peppermint, black pepper, laurel, orange, cinnamon, turmeric, white pepper, ginger, nutmeg, geranium, spearmint and ylang ylang. The method for inhibiting skatole production in the oral cavity is to apply the same in the oral cavity. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an agent that suppresses production of skatole, which is one of the main causative substances of bad breath, in the oral cavity.

  The malodorous components of bad breath include volatile sulfur compounds (VSC) such as methyl mercaptan, hydrogen sulfide and dimethyl sulfide, amines such as ammonia and trimethylamine, fatty acids such as valeric acid and butyric acid, phenol, It is said that there are phenols such as cresol and nitrogen-containing aromatic compounds such as indole and skatole.

  Among these malodorous components, VSC and amines tend to volatilize and can be easily detected by gas chromatography, sensors, etc., and are therefore often used as a target for malodor control research. For this reason, with regard to VSC and amines, as a main cause component of bad breath, many studies have been made on deodorization with a deodorant and growth inhibition of producing bacteria. In particular, VSC is considered to be highly related to periodontal diseases such as periodontal disease and caries, and many techniques related to growth control of deodorants and production bacteria targeting VSC have been reported.

  For example, in Patent Document 1, iron chlorophyllin sodium is effective for deodorizing trimethylamine and methyl mercaptan, and in Patent Document 2, a combination of polyphenols and polyphenol oxidase is effective for deodorizing VSC, trimethylamine, and ammonia. In fact, Patent Document 3 discloses that VSC is reduced by a combination of a Labiatae plant extract and lactoferrin.

  However, the present inventors paid attention to the fact that halitosis has an odor that is clearly different from VSC, and as a result of analyzing all components of halitosis, trimethylamine, dimethyl sulfide, p-cresol and skatole are the main causes of halitosis. It has been found that skatole is a substance, and in particular, skatole is greatly involved in the intensity and quality of bad breath (Patent Document 4).

  That is, as described above, since there are various substances in malodorous components of bad breath, even if only high volatile components such as VSC and amines are deodorized, low volatile phenols and Nitrogen-containing aromatic compounds remain. For this reason, it is not possible to reduce discomfort in the oral cavity only by deodorization of the above highly volatile components. In particular, unless the deodorization or reduction of skatole is realized, the bad breath and the discomfort in the oral cavity are fundamental. It cannot be improved.

  The present inventors can safely use in the oral cavity and are effective for deodorization of VSC, trimethylamine, etc., but the deodorization effect of skatole in the oral cavity has not been known, iron chlorophyllin sodium (patent document The skatole deodorizing effect of 1) and polyphenols (Patent Document 2) was examined, but it has been found that non-bromination is difficult.

  Because skatole is low in volatility, it is difficult to detect with a gas detector tube, etc., because it takes time to analyze each time with a commonly used instrumental analysis method such as gas chromatography, Since the amount of skatole remaining in the oral cavity is as low as 20 ppb or less, it is difficult to investigate the relationship between oral diseases and oral bacteria and odor components, and it has been attracting attention as a causative component of bad breath until now. There wasn't.

  In addition to the above circumstances, the production mechanism of skatole by oral bacteria has not been elucidated, and a material that is safe for use in the oral cavity and can effectively deodorize skatole has not been found Thus, an effective deodorizing method for skatole or an effective control technique for skatole producing bacteria has not been found so far.

JP-A-8-266604 Japanese Patent Laid-Open No. 10-212221 JP 2004-67530 A Japanese Patent Laid-Open No. 2004-205496

  As mentioned above, skatole is a component that contributes to the quality of bad breath, and in order to reduce bad breath and improve discomfort, the mechanism of skatole production is clarified and can be used safely in the oral cavity. There is a need to find materials that can suppress skatole production.

  Therefore, an object of the present invention is to provide a skatole production inhibitor that can be safely used in the oral cavity and can suppress the production of skatole by bacteria in the oral cavity.

  In order to elucidate the production mechanism of skatole, the present inventors have categorized the oral bacteria Prevotella, Porphyromonas, Fusobacterium, Hemophilus, Neisseria, Capnocytophaga, Bayonella, Actinobacillus. The skatole-producing ability of bacteria belonging to the genera, Actinomyces, Lactobacillus, Streptococcus, and Staphylococcus was examined. As a result, only a slight skatole-producing ability was found in Fusobacterium nucleatum and Prevotella intermedia. However, it was found that when lingual moss collected from a healthy person was added to Fusobacterium nucleatum, 40 to 50 times (400 to 500 times in terms of the number of bacteria) skatole was produced compared to Fusobacterium nucleatum alone.

  Generally, in addition to Fusobacterium (0.7%), obligate anaerobic gram-negative bacilli such as Prevoterra (8.2%) and facultative anaerobic gram-negative bacilli such as Haemophilus (3.2%) There are facultative anaerobic gram negative cocci such as Neisseria (3.4%), Streptococcus such as Streptococcus (38.3%), obligate anaerobic gram negative cocci such as Bayonella (16.0%) It is known that it is formed (Oral bacteriology discourse edition, 5th edition dentistry microbiology, medical and dental publishing, 1998, p.219-220).

  From the above, the present inventors have found that Fusobacterium nucleatum and the above bacteria present on the tongue co-aggregate to produce skatole. As a result of various searches for materials for suppressing skatole production by the mixed bacteria, it was found that a solvent extract of a specific plant (hereinafter referred to as a plant extract) can suppress the production of skatole in the oral cavity.

  The present invention is one or more selected from bay, fennel, savory, thyme, pimento berry, marjoram, estragon, peppermint, black pepper, laurel, orange, cinnamon, turmeric, white pepper, ginger, nutmeg, geranium, spearmint and ylang ylang It is intended to provide an oral skatole production inhibitor comprising the plant extract.

  Furthermore, this invention provides the skatole production suppression method in an oral cavity which applies the said skatole production inhibitor in an oral cavity.

  The skatole production inhibitor of the present invention can be safely used in the oral cavity, and can suppress skatole produced by the mixed bacteria present in the tongue coating and can fundamentally reduce bad breath.

  The skatole production inhibitor of the present invention includes a bay (Plumaceae), a fennel (Plumaceae), a savory (Lamiaceae), a thyme (Lamiaceae), a pimentberry (Pleurotus), a marjoram (Lamiaceae), an estragon (Asteraceae). ), Peppermint (Lamiaceae), Black Pepper (Pepperaceae), Laurel (Pinaceae), Orange (Rutaceae), Cinnamon (Pinaceae), Turmeric (Ginger family), White Pepper (Pepperaceae), Ginger (Ginger family) ), Nutmeg (Nutaceae), geranium (Auroaceae), spearmint (Lamiaceae), or ylang ylang (Ganodermaceae), one or more plant extracts are used, but for oral compositions and foods, etc. Peppermint, orange, gin, and so on when used because of less irritation such as pungent taste and strong smell Catcher over, spearmint, pimento berry is particularly preferred.

  The solvent used when obtaining the extract from the plant is preferably a solvent that can be used for food or oral products, such as water, ethanol, hexane, acetone, propylene glycol, and glycerin. Among these, it is preferable to use a solvent selected from water, ethanol, acetone, propylene glycol and glycerin, or a mixed solvent of two or more thereof. Furthermore, it is preferable to use a mixed solvent of water and ethanol, and the mass ratio is preferably water: ethanol = 90: 10 to 10:90, and particularly preferably water: ethanol = 60: 40 to 40:60.

  The extract by the above various solvents or mixed solvents is generally called oleoresin or extract. The obtained plant extract may be used as it is without distilling off the solvent, but it is preferable to use a product obtained by distilling off the solvent in order to increase the effectiveness.

  The obtained plant extract can be used as an emulsion by pulverization or mixing with a water-soluble component, an oil-soluble component or the like depending on the use. For pulverization, a normal pulverization method can be used. That is, a method of impregnating with an excipient such as dextrin or silica, a method of spray drying after mixing with dextrin, gum arabic, and water can be used.

  When preparing an emulsion by mixing a plant extract with a water-soluble and / or oil-soluble component, emulsifiers such as polyglycerin fatty acid ester and sugar ester can be used.

  Since the skatole production inhibitor of the present invention can be used safely in the oral cavity and is excellent in stability over time when blended into an oral product, an oral composition (mouthwash, spray, dentifrice) , Tablets, etc.), chewing gum, refreshing confectionery in the mouth, beverages, oral hygiene devices (dental floss, cloth, etc.) and the like.

  When mix | blending the skatole production inhibitor of this invention with an oral product, as for the content, 0.0000001-5 mass% is preferable in a product, and in order not to impair use feelings, such as a taste and fragrance of a product, 0.0000002-1 The mass% is more preferable.

Test Example 1 [Measurement of skatole-producing ability of oral bacteria]
According to the following method, oral bacteria were examined for skatole-producing ability.
To 2 mL of human sterilized saliva (sterilized saliva collected from a healthy person), Prevotella, Porphyromonas, Fusobacterium, Hemophilus, Neisseria, Capnocytophaga, Bayonella, Actinobacillus, 0.1 mL of each bacterial solution of the genus Actinomyces, Lactobacillus, Streptococcus, and Staphylococcus was added and cultured at 37 ° C. for 24 hours under anaerobic conditions. 0.5 g of sodium chloride was placed in a 10 mL test tube with a lid, and 1 mL of the culture solution was added thereto, and then 2 mL of a hexane / ether solution was added and mixed by shaking for 15 seconds. This was separated into an aqueous layer and an organic solvent layer with a centrifuge at 3500 rpm for 10 minutes, and the organic solvent layer was separated and subjected to GC-MS analysis.
Table 1 shows the ability of various oral bacteria to produce skatole. Among oral bacteria, Prevotella intermedia, Fusobacterium nucleatum, Fusobacterium periodonticum produced skatole of 0.5ppb or more. Note that the bacteria of the genus Haemophilus and Neisseria did not propagate under the aforementioned culture conditions.

Test Example 2 [Measurement of skatole-producing ability by mixed bacteria]
Saliva collected from healthy people is sterilized (sterilized saliva), and skatole-producing main bacteria Fusobacterium nucleatum (adjusted to an OD value of 1.0) are added. Fusobacterium nucleatum and tongue coating (OD) collected from healthy people Each of which was adjusted to a value of 0.1) was cultured at 37 ° C. for 24 hours under anaerobic conditions. 500 μL of the culture solution was collected, 500 μL of chloroform was added thereto, stirred and centrifuged, and then subjected to fluorescence liquid chromatograph (fluorescence HPLC) analysis.
Table 2 shows the amount of skatole produced when cultured with Fusobacterium nucleatum alone and the amount of skatole produced when cultivated with tongue coating added thereto. Fusobacterium nucleatum, which is a major skatole-producing bacterium, produces only about 1 ppb of skatole, whereas when tongue coating was added, skatole production was as high as 40 to 50 ppb. In addition, even though the OD value when adding tongue coating is 0.1 and 1/10 of the OD value of Fusobacterium nucleatum (1.0), skatole is produced. It was found that the skatole-producing ability when added was 400 to 500 times that of Fusobacterium nucleatum.

Examples 1 to 19 and Comparative Examples 1 to 4 [Measurement of ability of plant extract to inhibit skatole production]
After adding 5 μL of 1% ethanol solutions of various plant extracts to 500 μL of sterilized human saliva plus tongue coating, the cells were cultured at 37 ° C. for 24 hours under anaerobic conditions. Chloroform 500 μL was added to the culture solution, stirred and centrifuged, and then subjected to fluorescence HPLC analysis. A sample containing 5 μL of ethanol containing no plant extract and cultured was compared as a control.
As plant extracts, bay oleoresin (manufactured by Calsec), fennel oleoresin (manufactured by Calsec), savory oleoresin (manufactured by Bush Bork Allen), thyme oleoresin (manufactured by Dragoco), pimentoberry oleoresin (dibodan) ), Marjoram oleoresin (from Calsec), estragon oleoresin (from Dragoco), peppermint oleoresin (from Biolande), black pepper oleoresin (from Calsec), laurel oleoresin (from Dragoco), cinnamon Oleoresin (Biolande), Orange Absolute (SBI), Turmeric Oleoresin (Bushborg Allen), White Pepper Oleoresin (Calsec), Ginger Oleoresin (Calsec), Nutmeg Reorejin (Karusekku Co., Ltd.), (made by SBI, Inc.) geranium absolute, (manufactured by Biorande Co., Ltd.) spearmint oleoresin, with ylang ylang absolute (made by SBI, Inc.).
Table 3 shows the ability of each plant extract to inhibit skatole production. All plant extracts of Examples 1 to 19 inhibited the production of skatole as compared to the control (Comparative Example 1) to which no plant extract was added. Moreover, even if it compared with the celery (comparative example 2), the clove (comparative example 3), and basil (comparative example 4) said to be effective in suppression of bad breath, the ability to suppress skatole production was high.

Claims (4)

  One or more plant extracts selected from bay, fennel, savory, thyme, pimento berry, marjoram, estragon, peppermint, black pepper, laurel, orange, cinnamon, turmeric, white pepper, ginger, nutmeg, geranium, spearmint and ylang ylang An oral skatole production inhibitor comprising:   The skatole production inhibitor according to claim 1, wherein the plant extract is extracted using one or more solvents selected from water, ethanol, acetone, propylene glycol and glycerin.   The skatole production inhibitor according to claim 1 or 2, which suppresses the production of skatole by mixed bacteria present in tongue coating.   A method for inhibiting skatole production in the oral cavity, wherein the skatole production inhibitor according to any one of claims 1 to 3 is applied to the oral cavity.