JPH09502102A - テロメラーゼ活性アッセイ - Google Patents
テロメラーゼ活性アッセイInfo
- Publication number
- JPH09502102A JPH09502102A JP51399795A JP51399795A JPH09502102A JP H09502102 A JPH09502102 A JP H09502102A JP 51399795 A JP51399795 A JP 51399795A JP 51399795 A JP51399795 A JP 51399795A JP H09502102 A JPH09502102 A JP H09502102A
- Authority
- JP
- Japan
- Prior art keywords
- primer
- telomerase
- reaction mixture
- cell
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.細胞試料がテロメラーゼ活性を含有するかの判定方法であって、 (a)細胞試料から細胞抽出物を調製し; (b)テロメア繰返し配列を欠いているテロメラーゼ基質、およびテロメア繰 返し配列の付加によってテロメラーゼがテロメラーゼ基質の伸長を触媒すること ができる緩衝液からなる反応混合物中に細胞抽出物のアリコットを入れ、 (c)伸長テロメラーゼ基質が反応混合物中に存在する場合にテロメア繰返し と充分に相補的な配列からなるプライマーが該伸長テロメラーゼ基質にハイブリ ダイズし、伸長して、伸長テロメラーゼ基質の相補的コピーを形成するような条 件下、該反応混合物に該プライマーを添加して、それに特異的にハイブリダイズ させ、次いで、 (d)伸長プライマーと結合した伸長テロメラーゼ基質からなる二本鎖DNA 分子が存在する細胞試料におけるテロメラーゼ活性の存在と、二本鎖DNA分子 が存在しない細胞試料におけるテロメラーゼ活性の不在との相互関係を示すこと からなることを特徴とする、細胞試料がテロメラーゼ活性を有するかの判定方法 。 2.工程(c)が、さらに、 (1)反応混合物を加熱して、二本鎖DNA分子を変性させ;次いで、 (2)相補的核酸がハイブリダイズすることができ、かつ、伸長テロメラーゼ 基質が存在する場合にプライマーが伸長することができる温度に該反応混合物を 冷却する工程を含む請求項1記載の方法。 3.加熱および冷却工程が少なくとも5回繰り返され、該プライマーが各冷却 工程の間に伸長プライマーの形成のために充分な量で存在する請求項2記載の方 法。 4.鋳型依存性DNAポリメラーゼが反応混合物中に存在し、プライマーが、 DNAポリメラーゼにより、ヌクレオチドのプライマーへの添加によって伸長さ れる請求項2記載の方法。 5.鋳型依存性DNAリガーゼが反応混合物中に存在し、プライマーが、DN Aリガーゼにより、オリゴヌクレオチドリゴマーのプライマーへの結合によって 伸長される請求項2記載の方法。 6.熱安定性鋳型依存性DNAポリメラーゼが反応混合物中に存在し、プライ マーが、DNAポリメラーゼにより、ヌクレオチドのプライマーへの添加によっ て伸長される請求項3記載の方法。 7.熱安定性鋳型依存性DNAリガーゼが反応混合物中に存在し、プライマー が、DNAリガーゼにより、オリゴヌクレオチドリゴマーのプライマーへの結合 によって伸長される請求項3記載の方法。 8.細胞抽出物が非イオンまたは両性イオン洗浄剤からなる緩衝液中で細胞試 料中の細胞を溶解させることによって調製される請求項3記載の方法。 9.細胞試料がヒトの細胞試料である請求項3記載の方法。 10.プライマーが、最初に、ワックスバリヤーによって細胞抽出物と分離さ れており、次いで、反応混合物が加熱されて、該ワックスバリヤーが溶融され、 該プライマーが反応混合物に添加される請求項3記載の方法。 11.反応混合物が標識テロメラーゼ基質を含有する請求項3記載の方法。 12.反応混合物が標識プライマーを含有する請求項3記載の方法。 13.反応混合物が標識ヌクレオチド三リン酸を含有する請求項3記載の方法 。 14.テロメラーゼ基質およびプライマーが、プライマー伸長工程の間に該基 質と該プライマーとの二量体を形成するような結合を実質的に行わない配列を有 する請求項6記載の方法。 15.プライマーが該プライマーの5’−末端で非テロメア繰返し配列を有す る請求項6記載の方法。 16.プライマーが少なくとも2個のテロメア繰返し配列を有する請求項6記 載の方法。 17.プライマーおよびリゴマーが、最初に、ワックスバリヤーによって細胞 抽出物と分離されており、次いで、反応混合物が加熱されて、該ワックスバリヤ ーが溶融され、該プライマーが反応混合物に添加される請求項7記載の方法。 18.テロメア繰返し配列を欠いているテロメラーゼ基質がオリゴヌクレオチ ドTSである請求項9記載の方法。 19.プライマーがCXまたはACTである請求項9記載の方法。 20.標識が放射性原子、蛍光性原子、リン光性原子、レセプターに対するリ ガンド、ビオチン、およびアビジンからなる群から選択される請求項12記載の 方法。
Applications Claiming Priority (12)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/151,477 US5830644A (en) | 1992-05-13 | 1993-11-12 | Method for screening for agents which increase telomerase activity in a cell |
US08/153,051 | 1993-11-12 | ||
US08/151,477 | 1993-11-12 | ||
US08/153,051 US5645986A (en) | 1992-05-13 | 1993-11-12 | Therapy and diagnosis of conditions related to telomere length and/or telomerase activity |
US08/255,774 US5989807A (en) | 1992-05-13 | 1994-06-07 | Detecting cancerous conditions by assaying for telomerase activity |
US255,774 | 1994-09-28 | ||
US315,214 | 1994-09-28 | ||
US08/315,214 US5629154A (en) | 1993-11-12 | 1994-09-28 | Telomerase activity assays |
US153,051 | 1994-09-28 | ||
US08/315,214 | 1994-09-28 | ||
US08/255,774 | 1994-09-28 | ||
US151,477 | 1994-09-28 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10273689A Division JPH11243998A (ja) | 1993-11-12 | 1998-09-28 | テロメラーゼ活性アッセイ |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH09502102A true JPH09502102A (ja) | 1997-03-04 |
JP2875394B2 JP2875394B2 (ja) | 1999-03-31 |
Family
ID=27495974
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51399795A Expired - Lifetime JP2875394B2 (ja) | 1993-11-12 | 1994-11-10 | テロメラーゼ活性アッセイ |
JP10273689A Pending JPH11243998A (ja) | 1993-11-12 | 1998-09-28 | テロメラーゼ活性アッセイ |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10273689A Pending JPH11243998A (ja) | 1993-11-12 | 1998-09-28 | テロメラーゼ活性アッセイ |
Country Status (13)
Country | Link |
---|---|
US (1) | US5629154A (ja) |
EP (1) | EP0728207B1 (ja) |
JP (2) | JP2875394B2 (ja) |
AT (1) | ATE193554T1 (ja) |
AU (1) | AU682082B2 (ja) |
CA (1) | CA2173872C (ja) |
DE (1) | DE69424797T2 (ja) |
DK (1) | DK0728207T3 (ja) |
ES (1) | ES2147602T3 (ja) |
GR (1) | GR3034249T3 (ja) |
HK (1) | HK1011384A1 (ja) |
PT (1) | PT728207E (ja) |
WO (1) | WO1995013381A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014197008A (ja) * | 2005-04-01 | 2014-10-16 | メドベット サイエンス ピーティーワイ. リミティッド | 診断法および治療法ならびにそれに有用な薬剤 |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5693474A (en) * | 1992-05-13 | 1997-12-02 | Board Of Regents, University Of Texas System | Methods for cancer diagnosis and prognosis |
US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
US5863726A (en) * | 1993-11-12 | 1999-01-26 | Geron Corporation | Telomerase activity assays |
US5804380A (en) * | 1993-11-12 | 1998-09-08 | Geron Corporation | Telomerase activity assays |
US5876979A (en) * | 1994-07-07 | 1999-03-02 | Cold Spring Harbor Laboratory | RNA component of mouse, rat, Chinese hamster and bovine telomerase |
US6013468A (en) * | 1994-07-07 | 2000-01-11 | Cold Spring Harbor Laboratory | RNA component of telomerase |
US5698686A (en) * | 1994-10-20 | 1997-12-16 | Arch Development Corporation | Yeast telomerase compositions |
US5840490A (en) * | 1995-06-07 | 1998-11-24 | Mcmaster University | Telomerase activity associated with hematological and colorectal malignancies |
US5741677A (en) * | 1995-06-07 | 1998-04-21 | Geron Corporation | Methods for measuring telomere length |
WO1997015687A1 (en) * | 1995-06-07 | 1997-05-01 | Geron Corporation | Telomerase activity assays |
US6004939A (en) * | 1995-07-06 | 1999-12-21 | Ctrc Research Foundation Board Of Regents | Methods for modulation and inhibition of telomerase |
WO1997002279A1 (en) * | 1995-07-06 | 1997-01-23 | Ctrc Research Foundation | Methods and compositions for modulation and inhibition of telomerase |
US5856096A (en) * | 1995-09-20 | 1999-01-05 | Ctrc Research Foundation | Rapid and sensitive assays for detecting and distinguishing between processive and non-processive telomerase activities |
DE19644302A1 (de) * | 1995-11-28 | 1997-06-05 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis von Telomerase-Aktivität |
JPH09262100A (ja) * | 1996-03-27 | 1997-10-07 | Mount Sinai School Medicine City Univ New York | テロメラーゼ活性の存在を測定することによる尿試料中のヒト膀胱癌細胞の検出方法 |
JPH1052300A (ja) * | 1996-06-03 | 1998-02-24 | Kyowa Hakko Kogyo Co Ltd | 細胞内テロメラーゼ活性の測定法 |
US6489097B2 (en) | 1996-06-28 | 2002-12-03 | Chugai Seiyaku Kabushiki Kaisha | Method for the detection of telomerase activity |
JPH1028600A (ja) * | 1996-07-17 | 1998-02-03 | Kyowa Hakko Kogyo Co Ltd | テロメラーゼ活性の測定法 |
BR9711844A (pt) | 1996-10-01 | 2000-01-18 | Geron Corp | Preparação de proteìna isolada, substancialmente pura ou recombinante, proteìna de trt isolada, substancialmente pura ou recombinante protéina de trt isolada, substancialmente pura ou recombinante, protéina de trt isolada ou uma sua variante, ou um seu fragmento, polinucleotìdeo, sintético, substancialmente puro ou recombinante, ácido nucléico isolado, codificando uma proteìna de trt, vetor de expressão, célula transfectada, animal não-humano, ou sua progênie, animal não-humano transgênico, anticorpo ou seu fragmento de ligação, anticorpo, especificamente imunorreativo sob condições imunologicamente reativas, para uma proteìna de trt ou seu fragmento imunogênico, processos de determinar se um composto ou tratamento é um modulador de uma atividade ou expressão de trt, de determinar se um composto de teste é um modulador de uma atividade de trt, de preparar telomerase recombinante, de detectar um produto genético de trt em uma amostra, de detectar a presença de pelo menos uma célula humana positiva de telomerase em uma amostra biológica compreendendo células humanas, de diagnosticar uma condição relacionada com a telomerase em um mamìfero, para aumentar a capacidade proliferativa de uma célula de vertebrado in vitro, para detectar a presença de yuma sequência de polinucleotìdeo codificando pelo menos uma parte de uma trt em uma amostra bilógica, para produzir um polinucleotìdeo, para detectar a expressão ou presença de uma trt em uma amostra biológica, usos de ummpolinucleotìdeo, de um agente que aumenta a expressão ou atividade de uma trt, de um inibidor da expressão ou atividade de telomerase de uma proteìna, variante ou fragmento, composição farmacêutica, polipeptìdeo substancialmente purificado, sequência de polinucleotìdeo isolado, complexo de telomerase, composição, telomerase substancialmente pura, polipeptìdeo de trt isolado, ácido nucléico isolado. |
EP1164203B1 (en) * | 1996-11-06 | 2007-10-10 | Sequenom, Inc. | DNA Diagnostics based on mass spectrometry |
US7390891B1 (en) | 1996-11-15 | 2008-06-24 | Amgen Inc. | Polynucleotides encoding a telomerase component TP2 |
CA2274586A1 (en) | 1996-12-20 | 1998-07-02 | Geron Corporation | Methods for detecting and inhibiting the rna component of telomerase |
US5846723A (en) * | 1996-12-20 | 1998-12-08 | Geron Corporation | Methods for detecting the RNA component of telomerase |
DE19705071A1 (de) * | 1997-02-11 | 1998-08-13 | Guido Dr Krupp | Verfahren zur "Echtzeit-Analyse" von Telomeraseaktivität |
US6210915B1 (en) | 1997-12-31 | 2001-04-03 | University Of Texas Systems | Telomerase extraction method |
DE19804372A1 (de) * | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits |
EP1068296B1 (en) | 1998-03-31 | 2011-08-10 | Geron Corporation | Compositions for eliciting an immune response to a telomerase antigen |
US6017709A (en) * | 1998-04-29 | 2000-01-25 | University Of Houston | DNA replication templates stabilized by guanine quartets |
JP4013333B2 (ja) * | 1998-06-05 | 2007-11-28 | 株式会社B&Cラボラトリーズ | 皮膚の老化度の測定方法および測定用キット |
US6664046B1 (en) | 1999-12-16 | 2003-12-16 | Roche Molecular Systems, Inc. | Quantitation of hTERT mRNA expression |
EP1170378A1 (en) * | 2000-06-26 | 2002-01-09 | Boehringer Ingelheim International GmbH | Method for monitoring the effect of cancer therapies |
US20020045219A1 (en) * | 2000-08-02 | 2002-04-18 | Whitehead Institute For Biomedical Research | Production of human monoclonal antibodies |
US6428962B1 (en) | 2001-02-12 | 2002-08-06 | Dna Analysis, Inc. | Nucleic acid collection barrier method and apparatus |
EP1321768A1 (en) * | 2001-12-18 | 2003-06-25 | Roche Diagnostics GmbH | Diagnosis of endometriosis from menstrual blood |
AU2003208580A1 (en) * | 2002-02-05 | 2003-09-02 | Technion Research And Development Foundation Ltd | Lineage committed stem cells selected for telomerase promoter activity |
US20030232409A1 (en) * | 2002-03-11 | 2003-12-18 | Regents Of The University Of Minnesota | Immortal porcine cells |
AU2003290722A1 (en) * | 2002-11-12 | 2004-06-03 | Allied Biotech, Inc. | Methods and compositions for detecting telomerase activity |
ES2716528T3 (es) * | 2003-06-23 | 2019-06-13 | Telomerase Activation Sciences Inc | Composiciones para aumentar la actividad telomerasa |
WO2005000248A2 (en) | 2003-06-25 | 2005-01-06 | Geron Corporation | Compositions and methods for skin conditioning |
US20070122501A1 (en) * | 2003-06-27 | 2007-05-31 | Hong Kong University Of Science And Technology | Formulations containing astragalus extracts and uses thereof |
MXPA06002660A (es) | 2003-09-09 | 2006-09-04 | Geron Corp | Oligonucleotidos modificados para inhibicion de telomerasa. |
WO2006113470A2 (en) | 2005-04-15 | 2006-10-26 | Geron Corporation | Cancer treatment by combined inhibition of proteasome and telomerase activities |
DK2101789T3 (en) | 2006-10-30 | 2015-03-23 | Geron Corp | Combined telomerase inhibitor and gemcitabine for the treatment of cancer |
KR101560844B1 (ko) | 2007-06-04 | 2015-10-15 | 벤-구리온 유니버시티 오브 더 네게브 리서치 앤드 디벨럽먼트 어쏘러티 | 트라이-아릴계 화합물 및 이를 포함하는 조성물 |
US8374838B2 (en) * | 2007-10-22 | 2013-02-12 | The Wistar Institute | Method for identifying a compound that modulates telomerase activity |
SI2437606T1 (sl) | 2009-05-18 | 2016-10-28 | Telomerase Activation Sciences, Inc. | Sestavki in postopki za povečanje telomerazne aktivnosti |
US20120070842A1 (en) * | 2009-06-01 | 2012-03-22 | Harley Calvin B | Assay for Telomerase Activity Using Microfluidic Device |
WO2011106671A1 (en) * | 2010-02-26 | 2011-09-01 | Geron Corporation | Assay for telomerase activity |
CN103328652B (zh) † | 2010-04-16 | 2016-12-28 | 宙斯科学有限公司 | 测量用于测定未纯化的样品中细胞生活力的酶活性的方法 |
JP6113185B2 (ja) | 2011-12-22 | 2017-04-12 | ジェロン・コーポレーションGeron Corporation | テロメラーゼ基質およびテロメア長作用因子としてのグアニンアナログ |
WO2015048558A1 (en) | 2013-09-30 | 2015-04-02 | Geron Corporation | Phosphorodiamidate backbone linkage for oligonucleotides |
JOP20200257A1 (ar) | 2014-05-01 | 2017-06-16 | Geron Corp | تركيبات أوليجو نوكليوتيد وطرق لتحضيرها |
WO2018146689A1 (en) | 2017-02-12 | 2018-08-16 | Ben-Gurion University Of The Negev Research And Development Authority | Telomerase activating compounds for use in fertility and related applications |
IT201800002511A1 (it) | 2018-02-08 | 2019-08-08 | Univ Degli Studi Cagliari | Metodo per la misurazione dell’attività della telomerasi |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3942482A (en) * | 1974-10-09 | 1976-03-09 | Foster Wheeler Energy Corporation | Bayonet tube steam generator |
US4424279A (en) * | 1982-08-12 | 1984-01-03 | Quidel | Rapid plunger immunoassay method and apparatus |
CH663475A5 (de) * | 1983-09-02 | 1987-12-15 | Zellweger Uster Ag | Verfahren und vorrichtung zum programmieren von messgeraeten und reglern. |
JPS60135753A (ja) * | 1983-12-23 | 1985-07-19 | Koa Sangyo Kk | 非接触水分検知装置 |
JPS60161554A (ja) * | 1984-02-01 | 1985-08-23 | Furuno Electric Co Ltd | 塩分濃度計 |
US5310652A (en) * | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
US5188963A (en) * | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
US5334499A (en) * | 1989-04-17 | 1994-08-02 | Eastman Kodak Company | Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction |
US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
US5413924A (en) * | 1992-02-13 | 1995-05-09 | Kosak; Kenneth M. | Preparation of wax beads containing a reagent for release by heating |
US5489508A (en) * | 1992-05-13 | 1996-02-06 | University Of Texas System Board Of Regents | Therapy and diagnosis of conditions related to telomere length and/or telomerase activity |
US5523389A (en) * | 1992-09-29 | 1996-06-04 | Isis Pharmaceuticals, Inc. | Inhibitors of human immunodeficiency virus |
-
1994
- 1994-09-28 US US08/315,214 patent/US5629154A/en not_active Expired - Lifetime
- 1994-11-10 WO PCT/US1994/012951 patent/WO1995013381A1/en active IP Right Grant
- 1994-11-10 AT AT95903110T patent/ATE193554T1/de active
- 1994-11-10 DK DK95903110T patent/DK0728207T3/da active
- 1994-11-10 DE DE1994624797 patent/DE69424797T2/de not_active Expired - Lifetime
- 1994-11-10 JP JP51399795A patent/JP2875394B2/ja not_active Expired - Lifetime
- 1994-11-10 CA CA 2173872 patent/CA2173872C/en not_active Expired - Lifetime
- 1994-11-10 ES ES95903110T patent/ES2147602T3/es not_active Expired - Lifetime
- 1994-11-10 EP EP19950903110 patent/EP0728207B1/en not_active Expired - Lifetime
- 1994-11-10 PT PT95903110T patent/PT728207E/pt unknown
- 1994-11-10 AU AU12090/95A patent/AU682082B2/en not_active Expired
-
1998
- 1998-09-28 JP JP10273689A patent/JPH11243998A/ja active Pending
- 1998-11-30 HK HK98112561A patent/HK1011384A1/xx unknown
-
2000
- 2000-08-23 GR GR20000401936T patent/GR3034249T3/el unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014197008A (ja) * | 2005-04-01 | 2014-10-16 | メドベット サイエンス ピーティーワイ. リミティッド | 診断法および治療法ならびにそれに有用な薬剤 |
Also Published As
Publication number | Publication date |
---|---|
HK1011384A1 (en) | 1999-07-09 |
PT728207E (pt) | 2000-11-30 |
CA2173872A1 (en) | 1995-05-18 |
DE69424797D1 (de) | 2000-07-06 |
WO1995013381A1 (en) | 1995-05-18 |
AU1209095A (en) | 1995-05-29 |
DK0728207T3 (da) | 2000-10-02 |
EP0728207B1 (en) | 2000-05-31 |
ATE193554T1 (de) | 2000-06-15 |
CA2173872C (en) | 2001-08-28 |
JPH11243998A (ja) | 1999-09-14 |
US5629154A (en) | 1997-05-13 |
GR3034249T3 (en) | 2000-12-29 |
EP0728207A1 (en) | 1996-08-28 |
ES2147602T3 (es) | 2000-09-16 |
JP2875394B2 (ja) | 1999-03-31 |
DE69424797T2 (de) | 2000-12-28 |
AU682082B2 (en) | 1997-09-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPH09502102A (ja) | テロメラーゼ活性アッセイ | |
US5863726A (en) | Telomerase activity assays | |
US5837453A (en) | Telomerase activity assays | |
US5648215A (en) | Telomerase diagnostic methods | |
US5804380A (en) | Telomerase activity assays | |
Chadeneau et al. | Telomerase activity associated with acquisition of malignancy in human colorectal cancer | |
Van Steensel et al. | TRF2 protects human telomeres from end-to-end fusions | |
Ford et al. | Heterogeneous nuclear ribonucleoproteins C1 and C2 associate with the RNA component of human telomerase | |
US6514745B1 (en) | Oncoprotein protein kinase | |
Holt et al. | Comparison of the telomeric repeat amplification protocol (TRAP) to the new TRAP-eze telomerase detection kit | |
EP2094850B1 (en) | Cancer-related protein kinases | |
JP4068861B2 (ja) | 哺乳動物のテロメラーゼ | |
WO1995003323A9 (en) | Oncoprotein protein kinase | |
US7067283B2 (en) | Producing telomerase activators and inhibitors by screening with purified telomerase | |
US6706509B2 (en) | Oncoprotein protein kinase | |
Harada et al. | Telomerase activity in primary and secondary glioblastomas multiforme as a novel molecular tumor marker | |
Yoo et al. | Expression of telomerase activity, human telomerase RNA, and telomerase reverse transcriptase in gastric adenocarcinomas | |
JPH09500272A (ja) | 無限増殖能または腫瘍形成能を有するヒト細胞および動物細胞を同定する方法 | |
JPH11507839A (ja) | テロメラーゼ活性検定 | |
JP2004535157A (ja) | ケモカイン受容体と疾患 | |
Cheng et al. | Polymerase chain reaction‐based enzyme immunoassay for quantitation of telomerase activity: Application to colorectal cancers | |
AU723767B2 (en) | Telomerase activity assays | |
Maláska et al. | Telomerase activity and expression and telomere analysis in situ in the course of treatment of childhood leukemias | |
JP5312024B2 (ja) | ヒトがん細胞の不死化に関わる遺伝子およびその利用 | |
US6210915B1 (en) | Telomerase extraction method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080114 Year of fee payment: 9 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090114 Year of fee payment: 10 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090114 Year of fee payment: 10 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100114 Year of fee payment: 11 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110114 Year of fee payment: 12 |
|
S802 | Written request for registration of partial abandonment of right |
Free format text: JAPANESE INTERMEDIATE CODE: R311802 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110114 Year of fee payment: 12 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110114 Year of fee payment: 12 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120114 Year of fee payment: 13 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130114 Year of fee payment: 14 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130114 Year of fee payment: 14 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
EXPY | Cancellation because of completion of term |