JP6979940B2 - 粗さのある表面による核酸の迅速な単離のための方法および試験キット - Google Patents

粗さのある表面による核酸の迅速な単離のための方法および試験キット Download PDF

Info

Publication number
JP6979940B2
JP6979940B2 JP2018506477A JP2018506477A JP6979940B2 JP 6979940 B2 JP6979940 B2 JP 6979940B2 JP 2018506477 A JP2018506477 A JP 2018506477A JP 2018506477 A JP2018506477 A JP 2018506477A JP 6979940 B2 JP6979940 B2 JP 6979940B2
Authority
JP
Japan
Prior art keywords
nucleic acid
roughened
solid phase
aqueous solution
comb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018506477A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018520696A (ja
Inventor
ヒレブラント ティモ
シュトロー トアステン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AJ Innuscreen GmbH
Original Assignee
AJ Innuscreen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE102015211394.9A external-priority patent/DE102015211394B4/de
Priority claimed from DE102015211393.0A external-priority patent/DE102015211393A1/de
Application filed by AJ Innuscreen GmbH filed Critical AJ Innuscreen GmbH
Publication of JP2018520696A publication Critical patent/JP2018520696A/ja
Application granted granted Critical
Publication of JP6979940B2 publication Critical patent/JP6979940B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0854—Double walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2018506477A 2015-04-23 2016-02-26 粗さのある表面による核酸の迅速な単離のための方法および試験キット Active JP6979940B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE102015207481 2015-04-23
DE102015207481.1 2015-04-23
DE102015211393.0 2015-06-19
DE102015211394.9A DE102015211394B4 (de) 2015-06-19 2015-06-19 Vorrichtung und Verfahren zur Extraktion von Nukleinsäuren
DE102015211393.0A DE102015211393A1 (de) 2015-06-19 2015-06-19 Vorrichtung und Verfahren zur automatisierten Extraktion von Nukleinsäuren
DE102015211394.9 2015-06-19
PCT/EP2016/054178 WO2016169677A1 (de) 2015-04-23 2016-02-26 Verfahren und testkit zur schnellen isolierung von nukleinsäuren mittels rauer oberflächen

Publications (2)

Publication Number Publication Date
JP2018520696A JP2018520696A (ja) 2018-08-02
JP6979940B2 true JP6979940B2 (ja) 2021-12-15

Family

ID=55628983

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2018506478A Pending JP2018524017A (ja) 2015-04-23 2016-02-26 核酸を抽出する装置および方法
JP2018506479A Active JP6797185B2 (ja) 2015-04-23 2016-02-26 核酸を自動的に抽出する装置および方法
JP2018506477A Active JP6979940B2 (ja) 2015-04-23 2016-02-26 粗さのある表面による核酸の迅速な単離のための方法および試験キット
JP2022011151A Active JP7481376B2 (ja) 2015-04-23 2022-01-27 核酸を抽出する装置および方法

Family Applications Before (2)

Application Number Title Priority Date Filing Date
JP2018506478A Pending JP2018524017A (ja) 2015-04-23 2016-02-26 核酸を抽出する装置および方法
JP2018506479A Active JP6797185B2 (ja) 2015-04-23 2016-02-26 核酸を自動的に抽出する装置および方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022011151A Active JP7481376B2 (ja) 2015-04-23 2022-01-27 核酸を抽出する装置および方法

Country Status (8)

Country Link
US (3) US10851368B2 (en:Method)
EP (3) EP3286312B1 (en:Method)
JP (4) JP2018524017A (en:Method)
CN (3) CN108064262B (en:Method)
CA (3) CA2983619C (en:Method)
ES (2) ES2928055T3 (en:Method)
PL (2) PL3286312T3 (en:Method)
WO (3) WO2016169677A1 (en:Method)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3286312T3 (pl) 2015-04-23 2022-11-21 Ist Innuscreen Gmbh Urządzenie i sposób do ekstrakcji kwasów nukleinowych
DE102017204267B4 (de) * 2017-03-14 2021-05-27 Aj Innuscreen Gmbh Verfahren zur anreicherung von zellen aus einer probe und der nachfolgenden nukleinsäureisolierung aus diesen zellen
CN113227795B (zh) 2019-01-25 2024-09-03 积水化学工业株式会社 核酸的分离方法、核酸的分离试剂盒和检测芯片
DE102019201966B4 (de) * 2019-02-14 2023-07-27 Axagarius Gmbh & Co. Kg Probentransferwerkzeug
DE102019118332B4 (de) * 2019-07-07 2022-04-07 Ist Innuscreen Gmbh Verfahren und testkit zur bisulfitmodifizierung von dna
DE102020135124A1 (de) 2020-12-30 2022-06-30 Aj Innuscreen Gmbh Verfahren und System zur schnellen Isolierung von Nukleinsäuren direkt aus Vollblutproben
DE102021104319A1 (de) 2021-02-23 2022-08-25 Aj Innuscreen Gmbh Verfahren zur simultanen Isolierung von Nukleinsäuren aus sedimentierbaren und nicht sedimentierbaren Biomolekülen in Wasserproben
CN113546703A (zh) * 2021-07-30 2021-10-26 苏州含光微纳科技有限公司 一种离心式微流控芯片
DE102021130283B4 (de) 2021-11-19 2024-03-21 Ist Innuscreen Gmbh Verfahren und testkit zur preiswerten und ressourcensparenden extraktion von nukleinsäuren
CN113820195B (zh) * 2021-11-25 2022-03-08 成都宜乐芯生物科技有限公司 一种多通道并行前处理装置
DE102022115445B4 (de) 2022-06-21 2024-07-04 Ist Innuscreen Gmbh Verfahren und kit zur manuellen und automatisierten probenvorbereitung von long-read-sequenzierungen
CN115141719A (zh) * 2022-07-13 2022-10-04 安徽百奥秘科生物医药研究院有限公司 一种无阻碍吸入式动态快速提取核酸的方法及装置
DE102023136897B3 (de) * 2023-12-29 2025-05-22 Ist Innuscreen Gmbh Verfahren, mittel und kit zur extraktion hochmolekularer dna

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3717211A1 (de) 1987-05-22 1988-12-01 Diagen Inst Molekularbio Vorrichtung und verfahren zur trennung und reinigung von molekuelen
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5871928A (en) * 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5114858A (en) * 1990-06-26 1992-05-19 E. I. Du Pont De Nemours And Company Cellular component extraction process in a disposable filtration vessel
US5171537A (en) * 1991-05-06 1992-12-15 Richard E. MacDonald Activated immunodiagnostic pipette tips
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
DE4321904B4 (de) 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
JP3761573B2 (ja) 1994-06-14 2006-03-29 インヴィテーク ゲーエムベーハー 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法
JP3575116B2 (ja) * 1995-06-30 2004-10-13 日本製紙株式会社 印刷用紙及びその製造法
GB9517955D0 (en) * 1995-07-25 1995-11-08 Univ Strathclyde Nucleotide sequence detection and analysis
US6269846B1 (en) * 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
GB9810865D0 (en) * 1998-05-20 1998-07-22 Zeneca Ltd Nucleic acid sequence identification
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
WO2001005510A1 (en) 1999-07-19 2001-01-25 Akzo Nobel N.V. Device and method for mixing magnetic particles with a fluid
DE19937607A1 (de) * 1999-08-09 2001-02-15 Bilatec Ges Zur Entwicklung Bi Reagenzienkit zur Isolierung von Nukleinsäuren
DE29919506U1 (de) * 1999-11-05 2000-02-24 CREAVIS Gesellschaft für Technologie und Innovation mbH, 45772 Marl Mikrostruktuierte Pipetten als Dosiersysteme
AU2001238616A1 (en) 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
US6565728B1 (en) * 2000-06-08 2003-05-20 Elchrom Scientific Gel cutting and recovering device
AU2002225942B2 (en) * 2000-11-13 2007-05-10 Promega Corporation Lysate clearance and nucleic acid isolation using silanized silica matrices
JP2002212198A (ja) * 2001-01-15 2002-07-31 Jsr Corp 核酸の分離方法および核酸分離用担体
JP2003204799A (ja) * 2002-01-11 2003-07-22 Jsr Corp 白血球含有試料からの核酸分離方法
DE20207793U1 (de) 2002-05-17 2002-08-22 GL BioTech GmbH, 28359 Bremen Kit zur Durchführung eines Verfahrens zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung
US7722820B2 (en) 2004-11-19 2010-05-25 Phynexus, Inc. Method and device for sample preparation
US20080014610A1 (en) * 2004-06-02 2008-01-17 Arkray,Inc. Container for Nucleic Acid Extraction, Method of Cleaning Solid Matrix and Relevant Cleaning Mechanism, and Method of Purifying Nucleic Acid
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
DE102005053463A1 (de) * 2005-11-06 2007-05-10 Aj Innuscreen Gmbh Vorrichtung und Verfahren zur automatisierten Isolierung und Aufreinigung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
DE102005059217B4 (de) * 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
US20070281303A1 (en) * 2006-06-05 2007-12-06 Dna Security, Inc. Dna storage and display vessel and method
US20090111193A1 (en) 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US20110107855A1 (en) * 2008-03-28 2011-05-12 Pelican Group Holdings, Inc. Sample preparation devices and methods for processing analytes
CN101246178B (zh) * 2008-04-03 2010-05-12 马庆伟 用于吸附、分离、检测超微量靶蛋白的系统及其用途
US8105513B2 (en) * 2008-06-06 2012-01-31 Alexander Bonn Pipette tip containing particle-filled polymer monolith
WO2010024906A1 (en) * 2008-08-26 2010-03-04 Wako Pure Chemical Industries, Ltd. Disposable device for automated biological sample preparation
DE102008044721B4 (de) * 2008-08-28 2012-07-19 Forschungszentrum Jülich GmbH Verfahren zur Isolierung von Nukleinsäuren aus nukleinsäurehaltigen Proben sowie ein dazu geeignetes Testkit
CN102439128A (zh) * 2009-02-14 2012-05-02 第菲尼特基因组学公司 用于纯化生物物质的系统和方法
JP5789799B2 (ja) * 2009-08-21 2015-10-07 国立研究開発法人農業生物資源研究所 多孔質体の製造方法、細胞又は組織供給用支持体の製造方法、及び組織供給体の製造方法
DE102010031401A1 (de) * 2010-07-15 2012-01-19 Aj Innuscreen Gmbh Verfahren zur Anreicherung von Bakterien, Viren sowie Zellen und zur nachfolgenden Nukleinsäureisolierung
GB2502195B (en) * 2010-09-30 2015-02-11 Phynexus Inc Purification of nucleic acids
WO2014012079A1 (en) * 2012-07-13 2014-01-16 President And Fellows Of Harvard College Multifunctional repellent materials
JP6242896B2 (ja) * 2012-08-28 2017-12-06 アコーニ バイオシステムズ インコーポレイテッド 核酸を精製するための方法およびキット
AU2013202778A1 (en) * 2013-03-14 2014-10-02 Gen-Probe Incorporated Systems, methods, and apparatuses for performing automated reagent-based assays
PL3286312T3 (pl) 2015-04-23 2022-11-21 Ist Innuscreen Gmbh Urządzenie i sposób do ekstrakcji kwasów nukleinowych

Also Published As

Publication number Publication date
CA2983626A1 (en) 2016-10-27
CN108076644A (zh) 2018-05-25
US10934540B2 (en) 2021-03-02
EP3286312A1 (de) 2018-02-28
CA2983623A1 (en) 2016-10-27
ES2928505T3 (es) 2022-11-18
JP6797185B2 (ja) 2020-12-09
US10851368B2 (en) 2020-12-01
US20180133710A1 (en) 2018-05-17
CA2983626C (en) 2023-08-29
US10704039B2 (en) 2020-07-07
CN108076644B (zh) 2021-12-07
PL3286312T3 (pl) 2022-11-21
US20180148766A1 (en) 2018-05-31
CA2983619C (en) 2022-08-30
EP3286325B1 (de) 2022-07-13
JP2018520696A (ja) 2018-08-02
ES2928055T3 (es) 2022-11-15
CN108064262B (zh) 2021-08-31
CN108064262A (zh) 2018-05-22
PL3286325T3 (pl) 2023-01-23
CA2983619A1 (en) 2016-10-27
US20180148712A1 (en) 2018-05-31
CN108124454A (zh) 2018-06-05
JP7481376B2 (ja) 2024-05-10
WO2016169679A1 (de) 2016-10-27
JP2022058780A (ja) 2022-04-12
EP3286312B1 (de) 2022-07-20
WO2016169678A1 (de) 2016-10-27
JP2018524017A (ja) 2018-08-30
EP3286325A1 (de) 2018-02-28
EP3286313A1 (de) 2018-02-28
WO2016169677A1 (de) 2016-10-27
JP2018515132A (ja) 2018-06-14
CA2983623C (en) 2024-06-18

Similar Documents

Publication Publication Date Title
JP6979940B2 (ja) 粗さのある表面による核酸の迅速な単離のための方法および試験キット
JP5390772B2 (ja) 核酸を安定化試薬から精製するための組成物および方法
US20210163921A1 (en) Nucleic acid purification
EP2094846B1 (en) Use of tde for the isolation of nucleic acids
JP6440616B2 (ja) 高収量で小型ｒｎａを含むｒｎａを単離するための方法
JP2009131264A (ja) 核酸の単離および精製
EP2137309B1 (en) Methods for the separation of biological molecules using sulfolane
EP2479274B1 (en) Nucleic acid purification
JP2013528786A (ja) 核酸を単離および精製するためのクロマトグラフィーデバイスおよび方法
JP4441262B2 (ja) 改良された核酸の単離方法
EP3344643B1 (en) Methods for co-isolation of nucleic acids and proteins
CN110088282A (zh) 用磁性颗粒分离高纯度核酸的方法
US8067580B2 (en) Isolation of DNA, RNA and protein from a single sample
US10457931B2 (en) Purification of nucleic acid from a sample containing nucleic acid and endotoxin
US20080146789A1 (en) Methods for the separation of biological molecules using dioxolane
US8685742B2 (en) Apparatus and method for the more efficient isolation of nucleic acids
CN117377760A (zh) 用于直接从全血样品中快速分离核酸的方法和系统
KR20250139284A (ko) 핵산의 분리 및/또는 정제 방법

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190225

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200218

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200518

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200709

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200817

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201222

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210319

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210511

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210617

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20211018

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20211116

R150 Certificate of patent or registration of utility model

Ref document number: 6979940

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250