JP6974180B2 - 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法 - Google Patents

中脳ドーパミン(mDA)ニューロンのin vitro分化の方法 Download PDF

Info

Publication number
JP6974180B2
JP6974180B2 JP2017562028A JP2017562028A JP6974180B2 JP 6974180 B2 JP6974180 B2 JP 6974180B2 JP 2017562028 A JP2017562028 A JP 2017562028A JP 2017562028 A JP2017562028 A JP 2017562028A JP 6974180 B2 JP6974180 B2 JP 6974180B2
Authority
JP
Japan
Prior art keywords
cells
activator
signaling
days
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017562028A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018522538A (ja
JP2018522538A5 (https=
Inventor
ローレンツ ステューダー,
ステファン イリオン,
マーク トミシマ,
ソニア カークス,
Original Assignee
メモリアル スローン ケタリング キャンサー センター
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by メモリアル スローン ケタリング キャンサー センター filed Critical メモリアル スローン ケタリング キャンサー センター
Publication of JP2018522538A publication Critical patent/JP2018522538A/ja
Publication of JP2018522538A5 publication Critical patent/JP2018522538A5/ja
Priority to JP2020102332A priority Critical patent/JP7737787B2/ja
Application granted granted Critical
Publication of JP6974180B2 publication Critical patent/JP6974180B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0619Neurons
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48Reproductive organs
    • A61K35/54Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/14Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16Anti-Parkinson drugs
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702Regulators; Modulating activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/13Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/41Hedgehog proteins; Cyclopamine (inhibitor)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases [EC 2.]
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Neurosurgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Reproductive Health (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Engineering & Computer Science (AREA)
  • Psychology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017562028A 2015-06-01 2016-06-01 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法 Active JP6974180B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2020102332A JP7737787B2 (ja) 2015-06-01 2020-06-12 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562169444P 2015-06-01 2015-06-01
US201562169379P 2015-06-01 2015-06-01
US62/169,444 2015-06-01
US62/169,379 2015-06-01
PCT/US2016/035312 WO2016196661A1 (en) 2015-06-01 2016-06-01 Methods of in vitro differentiation of midbrain dopamine (mda) neurons

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020102332A Division JP7737787B2 (ja) 2015-06-01 2020-06-12 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法

Publications (3)

Publication Number Publication Date
JP2018522538A JP2018522538A (ja) 2018-08-16
JP2018522538A5 JP2018522538A5 (https=) 2019-07-04
JP6974180B2 true JP6974180B2 (ja) 2021-12-01

Family

ID=57441792

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2017562028A Active JP6974180B2 (ja) 2015-06-01 2016-06-01 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2020102332A Active JP7737787B2 (ja) 2015-06-01 2020-06-12 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2021205897A Withdrawn JP2022031961A (ja) 2015-06-01 2021-12-20 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2023188483A Withdrawn JP2023181494A (ja) 2015-06-01 2023-11-02 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2025197316A Pending JP2026015509A (ja) 2015-06-01 2025-11-18 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法

Family Applications After (4)

Application Number Title Priority Date Filing Date
JP2020102332A Active JP7737787B2 (ja) 2015-06-01 2020-06-12 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2021205897A Withdrawn JP2022031961A (ja) 2015-06-01 2021-12-20 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2023188483A Withdrawn JP2023181494A (ja) 2015-06-01 2023-11-02 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP2025197316A Pending JP2026015509A (ja) 2015-06-01 2025-11-18 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法

Country Status (18)

Country Link
US (3) US10858625B2 (https=)
EP (3) EP4070803B1 (https=)
JP (5) JP6974180B2 (https=)
KR (4) KR102358878B1 (https=)
AU (3) AU2016270793B2 (https=)
CY (1) CY1125423T1 (https=)
DK (2) DK4070803T3 (https=)
ES (2) ES2910032T3 (https=)
HR (1) HRP20220482T1 (https=)
HU (1) HUE058258T2 (https=)
IL (4) IL291532B2 (https=)
LT (1) LT3303564T (https=)
PL (1) PL3303564T3 (https=)
PT (2) PT3303564T (https=)
RS (1) RS63157B1 (https=)
SI (1) SI3303564T1 (https=)
SM (1) SMT202200263T1 (https=)
WO (1) WO2016196661A1 (https=)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6998772B2 (ja) * 2015-04-09 2022-02-04 ビオラミナ アーベー 神経変性疾患の処置における使用のための幹細胞由来のドパミン作用性細胞を生成するための方法および組成物
AU2016270793B2 (en) * 2015-06-01 2022-06-09 Memorial Sloan-Kettering Cancer Center Methods of in vitro differentiation of midbrain dopamine (MDA) neurons
KR20180105236A (ko) * 2016-02-05 2018-09-27 메모리얼 슬로안-케터링 캔서 센터 줄기 세포-유래 외배엽 계통 전구체의 분화 방법
KR102625865B1 (ko) * 2016-08-16 2024-01-16 후지필름 셀룰러 다이내믹스, 인코포레이티드 만능 세포를 분화시키는 방법
KR20190141218A (ko) * 2017-04-26 2019-12-23 메모리얼 슬로안 케터링 캔서 센터 사용 준비된 냉동보존 세포
WO2019016113A1 (en) 2017-07-17 2019-01-24 Miltenyi Biotec Gmbh METHOD FOR PROFILING EXPRESSION OF UNICELLULAR PROTEIN FROM DOPAMINERGIC PROGENITOR CELLS OF MESENCEPHALIC FLOOR
WO2019032680A1 (en) * 2017-08-08 2019-02-14 Regents Of The University Of Minnesota METHODS OF GENERATING AND USING ORGANOIDS AND ASSOCIATED CELLS
JP2022514298A (ja) 2018-12-19 2022-02-10 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ オルガノイドおよびその組織を作製および使用するための方法
WO2020237104A1 (en) * 2019-05-23 2020-11-26 The Mclean Hospital Corporation Autologous cell replacement therapy for parkinson's disease
JP7810357B2 (ja) 2019-07-25 2026-02-03 ザ スクリプス リサーチ インスティテュート ドーパミン作動性ニューロン及び前駆細胞を特定する方法
EP4021477A4 (en) * 2019-08-29 2023-12-13 Memorial Sloan Kettering Cancer Center METHOD FOR GENERATING AND ISOLATING MID-DOPAMINE NEURONS
EP4032974A4 (en) * 2019-09-20 2023-12-06 Korea Research Institute of Bioscience and Biotechnology Production method for induced dopaminergic neuronal progenitors, using direct reprogramming
KR20220158046A (ko) 2020-03-25 2022-11-29 사나 바이오테크놀로지, 인크. 신경계 장애 및 병태의 치료를 위한 면역저하성 신경 세포
WO2021216623A1 (en) 2020-04-21 2021-10-28 Aspen Neuroscience, Inc. Gene editing of lrrk2 in stem cells and method of use of cells differentiated therefrom
US20250263752A1 (en) 2020-04-21 2025-08-21 Aspen Neuroscience, Inc. Gene editing of gba1 in stem cells and method of use of cells differentiated therefrom
US12104170B2 (en) * 2020-06-26 2024-10-01 Minerva Biotechnologies Corporation Methods for deriving dopaminergic neurons from pluripotent stem cells
CN116635047A (zh) * 2020-06-26 2023-08-22 米纳瓦生物技术公司 用于从多能干细胞衍生多巴胺能神经元的方法
CN114292814A (zh) * 2020-09-22 2022-04-08 上海跃赛生物科技有限公司 一种多能干细胞分化为中脑黒质多巴胺能神经细胞的方法
WO2022216911A1 (en) * 2021-04-07 2022-10-13 FUJIFILM Cellular Dynamics, Inc. Dopaminergic precursor cells and methods of use
WO2023004370A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Aav-based modulation of gba1 and related compositions and uses thereof
WO2023004366A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Transposon-based modulation of gba1 and related compositions and uses thereof
WO2023086894A1 (en) 2021-11-12 2023-05-19 Axent Biosciences Inc. Methods for production of functional neurons
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
US12565639B2 (en) * 2023-06-13 2026-03-03 Aspen Neuroscience, Inc. Methods for differentiating dopaminergic neurons from stem cells
CN117448271B (zh) * 2023-12-22 2024-07-16 上海元戊医学技术有限公司 一种诱导多能干细胞分化为中脑多巴胺能神经前体细胞的方法
WO2025166063A1 (en) 2024-01-31 2025-08-07 Bluerock Therapeutics Lp Igfbp2 in cell culture
WO2025252180A1 (zh) * 2024-06-05 2025-12-11 中国科学院脑科学与智能技术卓越创新中心 中脑多巴胺能祖细胞群的体外制备方法
WO2026006245A1 (en) 2024-06-25 2026-01-02 Bluerock Therapeutics Lp Three-dimensional culturing of pluripotent stem cells for improved differentiability

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000068431A2 (en) 1999-05-06 2000-11-16 Mount Sinai School Of Medicine Of New York University Dna-based steganography
WO2001088104A2 (en) * 2000-05-17 2001-11-22 Geron Corporation Neural progenitor cell populations
EP1791949A2 (en) * 2004-09-02 2007-06-06 Neuro Therapeutics AB Methods and materials relating to enhanced production of dopamine neurons
WO2006071778A2 (en) * 2004-12-23 2006-07-06 Ethicon Incorporated Treatment of parkinson's disease and related disorders using postpartum derived cells
JP5649786B2 (ja) 2006-03-07 2015-01-07 ギータ シュロフ ヒト胚性幹細胞およびそれらの誘導体を含む組成物、使用方法、ならびに調製方法
WO2008008266A2 (en) 2006-07-07 2008-01-17 University Of Pittsburgh- Of The Commonwealth System Of Higher Education Biohybrid elastomeric scaffolds and methods of use thereof
WO2010096496A2 (en) 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
DK3578988T3 (da) 2010-05-25 2025-02-24 Memorial Sloan Kettering Cancer Center Fremgangsmåde til nociceptordifferentiering af humane embryonale stamceller og anvendelse heraf
US20110296542A1 (en) 2010-05-28 2011-12-01 Kevin Ka-Wang Wang Exogenous matrix-supported topical application of stem cells to organ surface
EP3693455A1 (en) * 2011-11-04 2020-08-12 Memorial Sloan-Kettering Cancer Center Midbrain dopamine (da) neurons for engraftment
WO2013188744A1 (en) 2012-06-15 2013-12-19 Baylor College Of Medicine Perineurium derived adult stem cells and methods of use
EP3498824A1 (en) 2013-04-26 2019-06-19 Memorial Sloan-Kettering Cancer Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
KR101696874B1 (ko) * 2013-07-31 2017-01-16 한국생명공학연구원 직접 리프로그래밍을 통한 유도 도파민성 전구세포 제조방법
EP3031908B1 (en) * 2013-08-06 2018-12-05 Takeda Pharmaceutical Company Limited Method for producing dopaminergic neurons
AU2014316100B2 (en) * 2013-09-05 2020-11-05 Eisai R&D Management Co., Ltd. New method for inducing dopamine-producing neural precursor cells
AU2014352773B2 (en) 2013-11-21 2021-04-29 Memorial Sloan-Kettering Cancer Center Specification of functional cranial placode derivatives from human pluripotent stem cells
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización
AU2016270793B2 (en) * 2015-06-01 2022-06-09 Memorial Sloan-Kettering Cancer Center Methods of in vitro differentiation of midbrain dopamine (MDA) neurons

Also Published As

Publication number Publication date
JP2018522538A (ja) 2018-08-16
DK3303564T3 (da) 2022-04-04
EP3303564B1 (en) 2022-02-23
KR20240101884A (ko) 2024-07-02
KR102679619B1 (ko) 2024-07-01
IL291532B2 (en) 2024-12-01
JP2023181494A (ja) 2023-12-21
AU2022228216B2 (en) 2026-02-19
JP2020146060A (ja) 2020-09-17
IL291532A (en) 2022-05-01
SI3303564T1 (sl) 2022-07-29
IL313799A (en) 2024-08-01
IL320440A (en) 2025-06-01
PL3303564T3 (pl) 2022-05-09
AU2016270793A1 (en) 2017-12-14
PT4070803T (pt) 2026-01-08
EP4070803A1 (en) 2022-10-12
HRP20220482T1 (hr) 2022-07-08
DK4070803T3 (da) 2026-01-12
AU2026200680A1 (en) 2026-02-19
JP2026015509A (ja) 2026-01-29
CY1125423T1 (el) 2024-12-13
WO2016196661A1 (en) 2016-12-08
EP4070803B1 (en) 2025-10-08
EP4079314A1 (en) 2022-10-26
KR102841407B1 (ko) 2025-08-01
SMT202200263T1 (it) 2022-07-21
US20240400987A1 (en) 2024-12-05
AU2016270793B2 (en) 2022-06-09
IL291532B1 (en) 2024-08-01
PT3303564T (pt) 2022-06-02
ES3057638T3 (en) 2026-03-03
IL255992A (en) 2018-01-31
IL255992B (en) 2022-04-01
KR20250118858A (ko) 2025-08-06
JP7737787B2 (ja) 2025-09-11
IL313799B1 (en) 2025-05-01
AU2022228216A1 (en) 2022-10-06
EP3303564A1 (en) 2018-04-11
HUE058258T2 (hu) 2022-07-28
JP2022031961A (ja) 2022-02-22
US20180094242A1 (en) 2018-04-05
IL313799B2 (en) 2025-09-01
ES2910032T3 (es) 2022-05-11
EP4079314B1 (en) 2025-10-29
KR20220020416A (ko) 2022-02-18
LT3303564T (lt) 2022-05-10
EP3303564A4 (en) 2018-10-31
RS63157B1 (sr) 2022-05-31
CA2987617A1 (en) 2016-12-08
KR102358878B1 (ko) 2022-02-08
KR20180014743A (ko) 2018-02-09
US10858625B2 (en) 2020-12-08
US12084679B2 (en) 2024-09-10
US20210123018A1 (en) 2021-04-29

Similar Documents

Publication Publication Date Title
JP6974180B2 (ja) 中脳ドーパミン(mDA)ニューロンのin vitro分化の方法
JP7759870B2 (ja) 中脳ドーパミンニューロンの生成及び単離方法
CA2987617C (en) Methods of in vitro differentiation of midbrain dopamine (mda) neurons
HK40081864A (en) Methods of in vitro differentiation of midbrain dopamine (mda) neurons
HK40082714A (en) Methods of in vitro differentiation of midbrain dopamine (mda) neurons
WO2026039433A1 (en) Midbrain dopaminergic neuron compositions and methods of use

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190531

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190531

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20200228

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200312

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200612

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201119

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210218

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210416

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210512

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20211012

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20211104

R150 Certificate of patent or registration of utility model

Ref document number: 6974180

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250